shoot tip culture - tissue culture
Post on 13-Apr-2017
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Shoot Tip
CultureDr. Amit Kumar Dutta,
Ph.D
Shoot Tip Culture may be described as the culture of terminal portion of a shoot comprising the meristem together with primordial and developing leaves and adjacent stem tissue.
Excised shoot tips and meristem can be cultured aseptically.
Grow out directly into a small leafy shoot or multiple shoots.
Young Twigs from a Healthy Plants
Sterilize(Surface)
Transfer each explant to a sterilized petriplate
Procedure
Remove the outer leaves
Apex is exposed
Cut off the apex
Procedure
Transfer to the Medium
Incubation(25OC & 16 Hrs Light)
Growing Single Leafy shoots or multiple shoots
Procedure
Transfer in Hormone free medium
Later on transfer into the pot under green House condition
Procedure
Application in T/C
Shoot tips & Meristem in tissue culture are very varied :-
Virus Eradication
Micro Propagation
Storage of Genetic Resources
Virus Eradication
Many Plants contain systemic viruses which substantially reduce their potential yield and quality.
Important to produce virus free stocks which can be multiplied.
Application in T/C
Micropropagation
A sexual or vegetative of whole plants using tissue culture techniques is referred to as micropropagation.
Shoot tip or meristem culture of many plant species can successfully be used for micropropagation.
Application in T/C
Storage of Genetic Resources
Many plants produce seeds that are highly heterozygous in nature or that is recalcitrant.
Seeds are not accepted for storing genetic resources.
The meristem from such plants can be stored in vitro.
Application in T/C
Shoot Tip or Meristem culture can also utilized in various important fields of plant science
Application in T/C
Shoot Tip or Meristem Culture and Plant Breeding
Propagation of Haploid Plants
Meristem or Shoot Tip Culture & Quarantine
Application in T/C
Thank You
Any Difficulties Contact to Respective
Teacher…….!
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