rotor-gene® q (qiagen) real time pcr detection system files/science/biology... · rotor-gene® q...
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Rotor-Gene® Q (Qiagen) Real Time PCR Detection System
Laboratory Name: Biochemistry & Molecular Biology Specialized Laboratory Lab Supervisor: Dr. Noura Abou Zeinab Location: Laboratory Building (A3), GF, Lab: G104-105, Debbieh Campus Tel. Ext.: 3363
The Rotor-Gene®Q instrument (SN: R121240)
with its Software (Version 2.3.1.49) is designed to
perform real-time and end-point thermal cycling
using the polymerase chain reaction (PCR) and
high-resolution melting analysis (HRM) in
molecular biology applications.
36-Well Rotor and 72-Well Rotor are available.
with the instrument. 36-Well Rotor Locking Ring
enables the use of 0.2 ml tubes. The 72-Well
Rotor and Locking Ring are used with Strip Tubes
and Caps, 0.1 ml, which can be used for volumes
as low as 20 µl. The caps provide a safe and
reliable seal.
Applications: It is highly suited to precisely
quantitate and analyze changes in gene
expression, genotyping, pathogen detection,
concentration measurement, protein analysis,
enzyme kinetics, and many other areas of
research. The powerful and user-friendly software
provides simplicity for beginners as well as an
open experimental platform for advanced users. The 36-Well Rotor. The 72-Well Rotor.
Applications for all thermal cyclers availabe (PX2, Q-Cycler, BIORAD)
PCR is widely used as a diagnostic and research tool. Its applications are widespread over many disciplines,
including molecular biology, microbiology, genetics, clinical diagnostics, forensic science, environmental science,
hereditary studies and paternity testing.
Copying and amplifying fragments of nucleic acids (DNA).
Analyzing clinical specimens for the presence of infectious agents, e.g. HIV, hepatitis, human papillomavirus
and others.
In forensic science, using PCR-based DNA fingerprinting, fragments of DNA can be isolated from a crime scene
and compared to a database of DNA of convicts or criminals.
DNA fingerprinting is also used in paternity testing.
Analysis of genetic mutations that occurs in cancer and many genetic diseases.
Assisting the DNA sequencing technique where segments of DNA from an area of interest can be easily
amplified to study their sequences.
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C1000TM Thermal Cycler PCR BioRAD
PCR Thermocycler Quanta Biotech Ltd (Q-Cycler)
The C1000 cycler (184-1000; SN: cc008327) cycler.
Dual 48/48 Reaction Module — It’s a Two Thermal
Cyclers in One. It offers a gradient-enabled dual
reaction module allowing to run two independent
experiments at the same time. It is convenient for
protocol optimization, allowing more variables to be
tested simultaneously
The Q-Cycler (7013000; SN: CM5057).
The Q-Cycler II independently controls and displays a real
time data for both itself and each connected satellite.
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Thermo- Px2 Thermal Cycler (Thermo Electron Corporation)
Thermo Electron’s Px2 Thermal Cycler – reliable PCR
performance (SN: Px220328; Numelab Company) Simple to
use, technologically advanced solution for thermal cycling
with: Accurate temperature control for optimal PCR
performance; Gradient capability for rapid protocol
optimization; Outstanding well to well uniformity for
reproducible PCR performance; High definition 1/4 VGA
display for enhanced user-friendliness; Keypad with
functional soft keys for easy software navigation
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Thermo Lbsystems Multiskan® EX - ELISA microplate reader
Thermo Lbsystems Wellwash® - ELISA washer
The Thermo Scientic Multiskan EX
(SN: 355-1-10183) is a basic reader
for endpoint and kinetic assays.
Additionally, the Multisikan EX with
onboard software (Ascent Software)
offers a wide range of data
reductions and report generations.
Absorbance Filters available: 405nm,
450nm, 540nm, and 620nm.
Applications: For reading 96-well
microplates, adapted ELISA and
other colorimetric assays and
immunological reactions.
Other applications include: Protein assays; Growth curve and hormone assays; Endotoxins; Food
diagnostics; HIV assays; Hybridization assays; Minisequencing assays; Cytotoxicity; Cell adhesion; Signal
transduction; Endotoxins; Antioxidants; Food diagnostics.
The Wellwash (SN: 006-1-10144) is a semiautomatic
microplate washer (96-well plates), comprising pump
and washer units. The instrument is supplied with
both 8- and 12-way interchangeable wash heads.
Applications: It can be used in research or routine-
test laboratories to wash microtiter microplates and
strips in 96-well plate format that meet the SBS
standards.
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GelDoc-It®2 & ChemiDoc-It®
2 Imagers
The GelDoc-It2 Imager is a high resolution imager,
capable of capturing, documenting and analyzing
fluorescent gel images, chemiluminescent blot
images, including Western blots. The system is
equipped with a cooled, scientific-grade CCD
camera.
Image incorporate a light-tight darkroom with
VisionWorks® software for image acquisition and
analysis functions. The full package VisionWorks™
L.S. Image acquisition and Analysis Software (SN:
478457-8246UL-34DDT2-4906zz) is included for
use on an external computer. Analysis functions
include: band analysis, histograms, lane profile
graphs, total area density, Western blot
densitometry and data reporting.
The darkroom has a UV-blocking gel viewer
window, built-in overhead epi white lighting, a UV
transilluminator and a four-position emission
filter wheel with an ethidium bromide (EtBr)
emission filter included as standard.
Applications: capturing, documenting and analyzing fluorescent gel images, as well chemiluminescent blot
images, including Western blots.
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HL-2000 HybriLinker Hybridization Oven
Ultracentrifuge (Beckman Coulter; OptimaL-100xP)
Innovative HL-2000 HybriLinkerTM System (SN: 053003-002; CA
91786) is designed with the combination benefits of a
Hybridization Oven and UV Crosslinker (254nm UV) in one self-
contained, space saving unit for crosslinking prior to
hybridization protocols.
The hybridization oven: is a cost-effective, high-performance,
10-bottle hybridization oven with microprocessor temperature
and variable speed controls. The Hybridizer can be set to
operate anywhere from 10 to 15 RPM. A touch-sensitive keypad
and microprocessor provide accurate temperature control from
ambient 10°C to 99.9°C. Two different hybridizer bottle sizes are
available for use within the hybridizer using the standard
carousel provided with the unit.
The crosslinker chamber: has shortwave 254nm, 8watt,
ultraviolet tubes supply uniform overhead UV illumination;
Preset and manual controls for ultraviolet or time exposures;
Preset exposure delivers 120,000 microjoules or five minutes of
exposure; Microprocessor measures and controls UV output.
Applications: Used for crosslinking of nucleic acids (DNA or RNA) to a nitrocellulose membrane, for hybridization
reactions in Southern and northern blotting assays, other UV Crosslinking.
Beckman Coulter; OptimaL-100xP ultracentrifuge
(SN: SERLxpo5G09; CA 94304) is capable of
generating maximum g- force 802,400xg (with a
maximum speeds up to 100,000 rpm0. Beckman
Coulter’s Ultracentrifuge enables researchers to
perform more separations in less time.
Applications: For proteomics, cytomics or
genomics, centrifugation of solutions containing:
RNA, DNA, microsomal p450 extraction and more.
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Cold Centrifuge-Centurion model K2015R – Bench top
Cold centrifuge Hero lab GmbH (UniCen 15DR)
Cold Centrifuge-Centurion model K2015R – Bench
top (SN: 217296-1): It has two rotors: BRK 5324 rotor
(capacity 24 x 2ml; For separation of solutions and
biological samples in a 1.5 ml tubes at 500-15000 rpm
at 40C - 250C) and BRK 5424 rotor (capacity 24 x 15 ml;
For separation of solutions and biological samples in
15 ml tubes at 500-6000 rpm at 40C - 250C).
HeroLab" Universal (Refrigerated) Benchtop Centrifuges
UniCen™ 15DR (SN: 0303715100): It is provided with two
rotors that can separate solutions and biological samples in
1.5-2 ml or 10 ml tubes at 5000 up to15300 rpm / 21,460 xg.
Temperature (Range from -10 to +40℃).
Applications of cold centrifuges: It is used to purify cells, subcellular organelles, viruses, proteins, and nucleic
acids (DNA and RNA).
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Thermo Scientific Heraeus Pico 17 Microcentrifuges
The Heraeus Pico17 (SN: 40651137) for routine laboratory work.
The 24-place rotor can be used for all 1.5 to 2.0 ml tubes, including
mini-preps and spin columns. It has a Max RCF 17,000 x g (Max
Speed 13,300 rpm); Process samples faster with unmatched
acceleration and deceleration rates.
Applications: For separation of solutions and biological samples. It
is used to purify cells, subcellular organelles, viruses, proteins, and
DNA.
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UV Spectrophotometer- UV/VIS (EvolutionTM 60,Thermo Scientific)
Spectro UV-2505 UV-Vis Spectrophotometer (LABOMED Inc)
The Evolution™ 60 (SN:2Q4M239002; CAT 840-189900) is a UV-Visible spectrophotometer with 6-position cell changer. The VISIONlite™ PC software provided offer intuitive method setup, data reporting, and advanced calculation capabilities. Biochemical tests are built into the Local Control software, and PC control analyzer packages are available for enzymatic food analysis, color calculations and materials calculations. The VISIONlite, consists of applications for wavelength scanning, fixed wavelength analysis, quantitative analysis and kinetics. Accessories provided: Rectangular Thermostatted single cell holder (Thermo Block; 840-206700 ;Thomas 1189N83) that is used to control the temperature of one 10 mm pathlength square cuvette within the range of the recirculator.
Applications: To determine the absorbance in solutions with wavelength range: 190 -1100nm in 1.5 ml to a
maximum 4 ml cuvettes; Protein/nucleic acid measurement; Kinetics measurement/analysis; Quantitative protein
analysis program with six different calibration methods.
Spectro UV-2505. Model UV-2505 works
in the ultraviolet and visible range of
195-1050nm and has a 4nm bandwidth.
Applications: Spectrophotometer Model
UV-2505 can be used extensively for
pharmaceutical manufacturing, health,
clinical laboratory, biochemistry,
petrochemistry, environmental
protection, quality control, water
management, food processing,
agriculture, and for a wide range of
businesses and industries.
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WiseTis – Homogenizer Wise Tis HG-15D
BANDELIN SONOPULS ULTRASONIC HD 2070 homogeniser
SONOPULS HD 2070 homogenizers
(SN:599.00045988.005) for use in laboratories
(For volumes from 1 ml to 200 ml). Ultrasonic
homogenizers in 2 sizes with 20 kHz
homogeneous sound and variable duration of
sonication, power output, and pulsing. With
integrated amplitude control 10-100 % and
actual value display, pulsation, AMPLICHRON®-
System, built-in timer, remote controlled.
Ready-to-operate basic equipment for volumes
from 2 ml to 50 ml
Applications: Emulsification; homogenizing of
substances; suspending; cell disruption
(bacteria, virus, tissues); degassing of fluids;
accelerating chemical reactions; sonochemistry;
sample preparation for HPLC; sample
preparation for particle size analysis; waste-
water analysis.
The digital laboratory device WiseTis HG-15D
(SN: HG15D081117001; set with WiseTis HG-15D,
dispersing tool HT1018, stand rodST110 and clamp CL200)
is built especially for exact homogenizing and is equipped
with high quality electronic controller.
Applications: Disruption of cells and tissues.
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SONIC RUPTUR 250 (OMNI INTERNATIONAL; The Homogenizer Compound)
The Omni Ruptor 250 Watt Ultrasonic Cell
Disrupter (SN: Z1876) is designed to handle a
variety of applications. Sample volumes can be
used with a range from 0.25 mL to 1000 mL
with the availability of different size solid
titanium tips. Pulse mode for sensitive samples;
0-15 minute adjustable timer; Auto-tuning for
convenience of use and optimal processing
efficiency; Power-emitted display for accuracy
and repeatability.
Applications: including disruption of cells,
bacteria, spores, emulsification of immiscible
liquids, homogenization, uniform dispersion of
particles in liquids, acceleration of reactions,
depolymerization of large molecules
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MUPID-exU Horizontal Electrophoresis System
Western Blotters (Vertical Gel Electrophoresis Unit)
OmniPAGE TETRAD Mini-Protein Electrophoresis
The MUPID-exU (SN:71295) is a submarine electrophoresis
system and includes a power supply, direct visible gel box with
high and complete UV-transmittancy. Multisample
analysis (support for multipipettors) multichannel compatible
gel loading combs (Allows simultaneous electrophoresis of 104
samples with a single gel), gel pouring stand, and a built in timer
(with alarm function) for set runs or continuous run modes (The
timer can be set within the range of 1-99 minutes). Variation of
input and output voltages: Input voltage supports 100-240V.
Output voltage can be selected from 7 levels.
Applications: For electrophoretic separation of DNA fragments and RNA molecules on agarose gels: Used in forensic medicine, molecular biology, genetics, biochemistry, microscopical biology; and Used in cloning, RFLP, PCR, and other molecular biology assays
OmniPAGE TETRAD Mini-Protein
Electrophoresis Package (SN: VS09072445)
for 1mm self-cast gels, Includes: CVS10DSYS-
CU– 10x10cm mini-vertical unit with casting
base and external casting stand, plus 2x1mm
12-sample combs, 2x plain glass plates with
1mm spacer and 2x notched plates.
Applications: For electrophoretic separation
of protein samples and for blotting proteins
on nitrocellulose, or PVDF membranes in
western blotting assays.
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Tissue Processor - HistoMaster 2052//2L/Z/A analog, MDS
Manual Rotary Microtome - microTec (Cut 4050)
Histomaster 2052/2L/Z/A analog system (SN: RW-
0909060) Download up to 380 cassettes; Easy
operation and maintenance; Possibility of
completing Rotary device for ease of maintenance;
With digital control.
The system possesses 12 stations, three paraffin
baths, and two metallic baskets with a maximum
loading up to 200 cartridges.
Applications: To prepare histological blocks from
different biological samples.
MicroTec CUT 4050 (SN: 500-410) Manual Rotary
Microtome with Oil, Dustcover & Tool Kit,
Including Knife Holder-Basic Unit, Disposable
Blade Holder, Clamping Plate-Low Profile,
Adapter-Orientable, Clamping Lever Left,
Universal Cassette Clamp and Section Waste Tray.
Applications: To cut thin slices of histological
blocks. Cuts range: starting from 1μm - 60μm
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Digital Cryo console Cold Plate Model ES 500–2
Paraffin Wax Dispenser (J.P.SELECTA Dispenser)
Cryo console (SN: CJHS0102ES500-2C-006) is one
component of the integrated embedding center.
The Cryo Console consists an advanced compressor
that can cool the paraffin blocks rapidly. The Teflon
coated surface guarantee a minimum of ice
formation and easy clean. Working temperature:
from 15℃ to -15℃, increments 1℃;Capacity: 70
cassettes
Applications: The Cryo console is used to chill the
paraffin blocks rapidly and to ensure the best
stability cooling performance. it can be used as a
standalone unit near microtome station and
embedding unit. It can be used with 60 cassettes.
Paraffin wax dispenser (SN: 0531589). Internal tank
made of aluminium coated with black indelible PTFE.
Independently heated tap.
Adjustable temperature from ambient +5 TO 100℃.
Stability ±1.5℃.
Applications: To dispense paraffin wax for the
preparation of different histological blocks with
temperatures of 00C -1000C.
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Thermostatic Baths: Paraffin wax floating out bath for histology
samples “Termofin” (J.P.SELECTA, Thermofen)
Forma (-860C)- Ultra-Low Temperature Upright Freezers (Thermo)
Paraffin wax floating out bath (SN:
0531716): it is a Bath and lid made of black
PTFE.
Adjustable temperature from 40°C TO
80°C. Stability ±1 °C.
Applications: Suitable for the fusion and
preservation of paraffin wax in a liquid
state that enables the users to see the
floated sections.To place slices of the tissue
embedded paraffin ribbons in water bath
(00C -1000C).
Thermo Scientific Forma freezers (SN:804603).
Applications: To store specimens, tissues, and reagents at ~
-700C.
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Hot Drying Oven (CONTHERM)
The Contherm Thermotec Designer Series Ovens (CAT 8050-8150, SN: 07561, 50L capacity; and SN: 08306,
100 L capacity).
Applications: To dry samples, glasswares, and to melt wax. Temperature range: 200C -1000C
The Contherm Thermotec Designer Series Ovens
(CAT 8050-8150, SN: 07561, 50L capacity)
The Contherm Thermotec Designer Series Ovens
(CAT 8050-8150, SN: 08306, 100 L capacity)
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Laboratory Name: Central Laboratory Of Biochemistry & Molecular Biology Lab Laboratory Name: Central Laboratory Of Biochemistry & Molecular Tel. Ext.: 3363 Tel. Ext.: 336
Steam Sterilizer
Incubator
Laboratory Name: Specialized Microbiology Laboratory Lab Supervisor: Mrs. Lama Fayad Location: Laboratory Building (A3), GF, Lab: G110, Debbieh Campus Tel. Ext.: 3365
Steam Sterilizer (Raypa, 36615), is used to sterilize buffers, solutions, culture media, glassware, surgical instruments, plastic wares, equipments, medical and pathogenic wastes by subjecting them to high-pressure saturated steam at 121 °C for around 15–20 minutes. Applications Steam Sterilizer is a simple yet very effective decontamination method; it is widely used in microbiology, medicine, dentistry and other fields.
Incubator (Memmert & Wtc binder, E306.0152 & 920378) is a device used to grow and maintain microbiological cultures or cell cultures by maintaining optimal temperature, humidity and gaseous content of the atmosphere inside. Applications Incubators are essential for a lot of experimental work in cell biology, microbiology and molecular biology.
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Shaking Incubator
Cooling Incubator
Shaking Incubator (ZHWY SERIES DOUBLE- LAYER CONSTANT TEMPERATURE) can be used for growth of bacterial and fungal cultures at different rpm (from 30 to 300 rpm) and temperatures (from 4°C to 60°C). Applications It has a wide range of application in research of medicine, biology, pharmacy and environmental research. It is used for cell culturing, cell aeration, solubility studies and ELISA incubations.
Cooling Incubator (STIK, 012080027) is intended for environmental test, thermostat test, low temperature preservation. It is used for applications that require cold temperatures, in the range of room temperature and below down to sub-zero temperatures (-10°C). Applications It is widely used in research, drug testing, agricultural research, and environmental protection fields.
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Centrifuge
Nitrogen Distiller
Centrifuge (CENTURION, 13762-1) is used for routine
laboratory work. It has a Max RCF 13500 g; max speed
12000 rpm; various acceleration and deceleration rates.
Applications
Used for separation of solutions and biological samples.
This apparatus is found in most laboratories of academic
and clinical researches. It is used to purify cells,
subcellular organelles, viruses, proteins and DNA.
Nitrogen Distiller (RAYPA, DNP- 2000) The distillation unit is used to perform nitrogen analysis and protein determination according to Kjeldahl method, in cells, food industry and other applications in environmental and chemical industries after having digested the sample accurately.
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Fermenter
Vacuum Freeze Dryer
Fermenter (BIOTRON, GR-1002F-06) is used for the cultivation of microorganisms that produce hormones, vaccines, antibiotics, vitamins, enzymes, and other metabolites. It provides a controlled environment for optimal biomass/product yields. It is used for large cultures containing 3 liters of growth medium. Applications Fermenters are made for research and production in biotechnology institutes, universities, biochemical industries, food, agricultural, pharmaceutical industries, and life science industries. They maintain optimal conditions for the growth of microorganisms.
Vacuum Freeze Dryer (BOYIKANG, FD-ID-50) is a dehydration technique, which enables liquid or slurry products, which have previously been frozen to be dried under a vacuum. Of the various methods of dehydration, freeze drying is especially suited for substances that are heat sensitive.
Applications
Vacuum Freeze Dryer is used for preservation of temperature sensitive products, such as enzymes, blood plasma, vaccines, etc, and for conversion of suspension and liquid samples to powder at -200°C. It is used to improve storage life and improve marketing of the end product. Also, vacuum Freeze Dryer is an important process in sample preparation and for the preservation and storage of biological, pharmaceuticals and foods, it has been extensively used in the development of pharmaceuticals (e.g., antibiotics) and preservation of biological substances (e.g., proteins, plasma, viruses and cell lines).
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Computerized Spectrophotometer
Natural Convection Oven
Colony Counter
Computerized Spectrophotometer (JASCO
RESEARCH GRADE V-630 UV/VIS, C284961148) works
in the ultraviolet and visible range of 190-1100 nm.
Applications
It can be used extensively for pharmaceutical
manufacturing, health, clinical laboratory,
biochemistry, petro chemistry, environmental
protection, quality control, water management, food
processing, agriculture, and for a wide range of
businesses and industries.
Natural Convection Oven (SHINSAENG SDON-301, TIG22-SDON301-01) is a hydraulic thermostat provides reliable temperature control with a range from 5°C above ambient to 210°C. Applications It is used for sterilization, drying, and thermal storage tasks that do not require high drying rates.
Colony counter (J-2 Colony counter semi automatic digital) is a device used for counting colonies of bacteria growing in a culture and to calculate the bacterial concentration in samples. It is used also to evaluate the quality of food and water.
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