reproduction of the amphiprion ocellaris clownfish vs amphiprion clarkii clownfish
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Reproduction of the Amphiprion ocellaris clownfish vs Amphiprion
clarkii clownfish
By Amelia Coletti and Kelly Peterson
Focus of Research
• Reproduction cycle of the Amphiprion ocellaris clownfish vs. Amphiprion clarkii clownfish
Tank 90 mated pairT
Tank C2 mated pair
IntroductionBackground of the
anemonefishes-Clownfishes by Joyce
Wilkerson, 2001 -Clownfishes by
Richard Stratton, 2000
-Scott Michael, Clownfish Breeding, 2012 Tank 90 mated pair
Tank C2 mated pair
Introduction
Research Questions1. Does the type of lighting affect the reproduction of clownfish? (moonlights encourage
spawning.)
2. Are the clownfish more likely to lay their eggs on a surface covered in algae, like the rock in the aquarium, or a clean surface like a tile?
3. Do the Amphiprion Clarkii breed less or more than the Amphiprion ocellaris?
4. Do environmental factors like water temperatures, water currents, pH, and lighting have an affect on the hatching of the clownfish eggs? (which would take place in a separate tank)
5. Does the Amphiprion ocellaris have different reproductive behaviors then the Amphiprion Clarkii? ex( male cleaning the rock, tattered fins on the female from the male nipping at her)
Introduction
Hypothesis:The Amphiprion ocellaris clownfish breeds
more often than the Amphiprion clarkii clownfish due to the effects of the addition of a flat and clean surface to spawn on, and the day and lunar lighting in tank 90 than in tank C2.
Methods- tank 90
1. Amphiprion ocellaris clownfish
2. Tile for the clownfish to lay eggs on
3. Moonlighting / lunar lighting4. Hydrometer, thermometer,
pH test kit5. Movement of Powerheads6. Mirror type tool to view the
back of the rock
TIle addition
Lunar lighting
Methods- Tank C21. Amphiprion clarkii 2. Fake anemone3. Mirror type tool to view
the back of the rock4.Hydrometer, thermometer, pH
test kit
Artificial anemone
Xenia and Clarkii
Methods- Data Collection
-Mon, Tue, Wed, Thur, and Fri : Daily observations in tank 90.
-Tuesday, and Thursday: Observations were taken on the C2 tank.
Data collection in tank 90
-Behavior changes-Location of the male and the female -Conditions within the tank -Algae Growth-Reproduction behavior before the addition of
lunar lighting / after
Data collection in Tank C2
-Effects of artificial anemone -Behavior of male and female clarkii -Location of mated pair within the tank -Algae growth -Used mirror to see behind the rocks
Results
Research Questions1. Does the type of lighting affect the reproduction of A.clarkii and A.ocellaris?
(moonlights encourage spawning.)
2. Are the A.ocellaris and the A.clarkii more likely to lay their eggs on a surface covered in algae, like the rock in the aquarium, or a clean surface like a tile?
3. Do environmental factors like water temperatures, water currents, pH, and lighting have an affect on the hatching of the clownfish eggs? (which would take place in a separate tank)
4. Do the Amphiprion clarkii breed less or more than the Amphiprion ocellaris?
5. Does the Amphiprion ocellaris have different reproductive behaviors then the Amphiprion Clarkii?
Result Tank 90
1st stage of larvae reproduction
2nd stage of larvae reproduction
3rd part of larvae reproduction
4th stage of larvae reproductionThe Scar
Result Tank 90
- Anemone
Anemone protecting eggs
Results tank 90
Representation of data
Month Number of Days since last laid eggs
12/20 zero (that we know of)
2/21 63 days
3/22 29 days
4/17 26 days
Note: Turned on lunar lighting on 2/28
Reproduction Rate:
Data Chart for tank 90
Data for tank 90
-4 days of being orange
-6 days dark gray but turning silver
-1 day- completely silver- hatch that night
March videos: March videos
Results in tank C2 -Artificial anemone effects:-Xenia-No signs of reproduction
Algae bloom in tank Xenia reacts to artificial anemone
Discussion: Reflection of Hypothesis
Support or Reject?The Amphiprion ocellaris clownfish breeds
more often than the Amphiprion clarkii clownfish due to the effects of the addition of a flat and clean surface to spawn on, and the day and lunar lighting in tank 90 than in tank C2.
Challenges to research project
Tank90-Could not observe tank 24/7-Presence of numerous organisms-Could not save any eggs
Tank C2-Hardly any changes in behavior -Set up of tank-Lack of organisms -Algae blooms -No lunar lighting in tank
How can we make this research more complete? /Future directions
-Be present at night -Have the eggs easy to remove-Put mated pair in a secluded tank, to decrease
threat-Put lunar lighting system on C2 rack-Put an anemone in tank c2
Application/ Value of results
-Lunar lighting has a major impact in the reproduction cycle
-The presence of an anemone provides protection for eggs and mated pair, increasing reproduction
Bibliography
1. "Aquarium Fish: Reproduction and rearing of ocellaris clownfish (Amphiprion
ocellaris) in captivity." advanced aquarist. N.p., n.d. Web. 2 Oct. 2012.
<__http://www.advancedaquarist.com/2007/2/fish>.__
2. Breeding Clownfish. N.p., n.d. Web. 21 Oct. 2012.
<__http://www.breedclownfish.com/>.__
3. Clownfishes and Sea Anemones. N.p.: Barron's, 1998. Print.
Hoff, Frank H. Conditioning, Spawning, and Rearing of Fish with Emphasis on Marine Clownfish. 3rd ed. Dade City: Florida Aqua Farms, 2009. Print.
4 Kurtz, Jeff. Clownfishes and Other Damselfishes. Neptune City: TFH, 2010. Print.
5.Michael, Scott W., ed. Clownfish Breeding. N.p., n.d. Web. 21 Oct. 2012.
<http://www.fishchannel.com/saltwater-aquariums/species-info/clownfish/
breeding-clownfish-2.aspx>.
6. "Ocellaris Clownfish." The Home Aquarium. N.p., n.d. Web. 21 Oct. 2012.
<tp://www.thehomeaquarium.com/ocellaris-clownfish/>.
7. Stratton, Richard F. Clownfishes N.p: 2000 Print
8. Wilkerson, Joyce D. Clownfishes. Neptune City: T.F.H., 2001. Print.
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