protein / protein interface design - cyrus bio21 protein / protein interface design...
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Protein / Protein Interface DesignExample: Bispecific IgG Antibodies Orthogonal Fab Interface Design
Lewis et al., Nature Biotechnology, 2014
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Correct Assembly Incorrect Assembly Incorrect Assembly
CH11-CH12/CL1-CL2 CH11-CH12/CL1-CL1 CH11-CH12/CL2-CL2
Accuracy of IgG BsAb Assembly2 ml Culture
CH11 (Pertuzumab) CL1 (Pertuzumab)
VHVRD1CH1CRD2 VLVRD1CλCRD2
CH12 (Matuzumab) CL2 (Matuzumab)
VHVRD2CH1WT VLVRD2CκWT
CL
10 ways for 2 unique L+H chains to come together. Only one needed.
CH1
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