production of proteins from cloned genes - ifm · production of proteins from cloned genes terms:...

Production of proteins from clonedgenes

Terms:Expression vectorsFusion proteinsStrong/weak promotersGlycosylation patternCell-free translation

Cloning for protein produktion

Fig 13.1 p226

Production of eukaryotic proteins in E.coli

Fig 13.2 p227

Signals for gene expression

Fig 13.3 p228

Typical Promoter sequences

Fig 13.4 p228

Expression vector for expression of foreign genes in E.coli

Fig 13.5 p229

Strong/weak promoters

Fig 13.6 p230

Gene regulation

Fig 13.7 p230

Promoters in expression vectors

Fig 13.8 p231

Cassette vector

Fig 13.9 p232

Translation of fusion protein

Fig 13.10 p233

Problem with secondary structureat the start of transcription

Fig 13.11 p233

Affinity chromatografy for purification of Glutation-S-transferas

Fig 13.12 p234

Cleavage of a fusion protein

Fig 13.13 p234

Problem with expression of foreignproteins in E.coli

Fig 13.14 p235

Inclusion bodies

Fig 13.15 p236

Promoters for eukaryoticexpression vectors

Fig 13.16 p238

Glycosylation pattern

Fig 13.17 p239

Inclusion bodies in insect cells

Fig 13.18 p240

Cell free translation system

Cell-free translation

Figure 11.18p200