plant physiology archive complete - the plant cell

Plant Physiology is planning a special issue devoted toReactive Oxygen Species in June 2006. All excellent paperscovering reactive oxygen species signaling and metabolism,oxidative stress homeostasis, and redox signaling research inplants will be considered.

Authors interested in contributing to this special issue shouldindicate this in their cover letter when submitting papers online at

http://submit.plantphysiol.org/. Manuscripts to be considered forthe special issue should be submitted no later than January 31, 2006.

For additional information, please contact Editor-in-Chief Donald Ort(d-ort@uiuc.edu) or Special Issue Editors Julia Bailey-Serres

(serres@ucr.edu) and Ron Mittler (ronm@unr.edu).

Plant PhysiologyAnnounces a Reactive Oxygen Species Special Issue

The Open Access movement in scholarly publishing advocates that research content shouldbe freely available to all immediately upon publication. This approach has prompted publish-ers to examine the feasibility of a shift from traditional subscription-based (“user pays”)financial models to an “author-pays” model, in which some or all of the costs of publicationare typically borne by authors.

What does our author community think about Open Access? To gauge the plant sciencecommunity’s interest in this new approach to publishing and to help ASPB determine theviability of “author-pays” publishing models, the Society is conducting an 18-month OpenAccess “experiment.” Beginning with the December 2005 issues of The Plant Cell and PlantPhysiology, authors of articles accepted by the journals will be given the option to pay a sur-charge to make their online article free from the moment of publication to anyone withInternet access. The surcharge, which is in addition to the usual author charges, will be$1,000 (discounted to $500 if the author’s institution subscribes to the journal).

For more information, go to http://www.aspb.org/publications/openaccess.cfm or contactNancy Winchester, ASPB director of publications, at nancyw@aspb.org.

Plant Cell Lysis

www.piercenet.com/pper38j

The P-PER® Reagent Kit † (#89803) extractsmaximum active protein from stem, root, seed andleaves in 10 minutes without liquid nitrogen.

You can trust the experts at Pierce to know how to get max-imum protein extraction from your sample. We have beenthe protein people for over 50 years, providing the bestsolutions to extract, purify and quantify proteins from manytypes of cell lines and tissue.

Is your plant cell lysis leafing you out in the cold?

Fresh leaf tissue and seed were lysed and extracted according to the P-PER® Kit (Product # 89803)protocol, a competitor’s protocol and a literature-based (home brew) protocol. Samples were normalized(weight tissue/volume extract), resolved on a 10% Bis-Tris gel and stained with Imperial™ ProteinStain† (Product # 24615). Samples were also quantified using the BCA™ Protein Assay Kit, ReducingAgent Compatible (Product # 23250).

*The Competitor S kit is not recommended for dried seed.

Tel: 815-968-0747 or 800-874-3723 • Fax: 815-968-7316 • Customer Assistance E-mail: CS@piercenet.comOutside the United States, visit our web site or call 815-968-0747 to locate your local Perbio Science branch office (below) or distributor

© Pierce Biotechnology, Inc., 2005. Pierce products are supplied for laboratory or manufacturing applications only.

BCA™, P-PER® and Imperial™ are trademarks of Pierce Biotechnology, Inc. † U.S. patents pending on P-PER® Technology, Imperial™ Protein Stain and Reducing Agent-compatible BCA™ Technology.

Belgium & Dist.:Tel +32 53 85 7184

euromarketing@perbio.com

China:Tel +86 10 8049 9033support@perbio.com.cn

France:Tel 0800 50 82 15

euromarketing@perbio.com

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de.info@perbio.com

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euromarketing@perbio.com

United Kingdom: Tel 0800 252185

uk.info@perbio.com

Switzerland: Tel 0800 56 31 40

euromarketing@perbio.com

P-PER® Reagent Kit Highlights:

• Convenient – disrupts cells without harsh mechanical methods in 10 minutes

• Compatible – downstream applications include 1-D and 2-D gel electrophoresis,Western blotting, activity assays, and protein affinity purifications

• Quantifiable – use the BCA™ Protein Assay Kit, Reducing Agent Compatible†

• Ready-to-use – extract does not require filtration through cheesecloth or Miracloth

• Provides active proteins – extracted proteins are functional

New! Poppers™ Cell Lysis Solutions HandbookLog on to our web site or call us to requestyour FREE handbook (#1601234). Outsidethe U.S., contact Perbio Science or your local distributor.

200

976643

29

2014

6

3.6

0.02 0.016 0.009

Leaf

MW (kD)

Protein extracted (mg)/ total tissue weight (mg)

Pie

rce

Tobacco

Hom

e B

rew

Com

pet

itor

S

0.02 0.02 0.01

Pie

rce

Hom

e B

rew

Com

pet

itor

S

0.176 0.09

Pie

rce

Hom

e B

rew

0.056 0.046

Pie

rce

Hom

e B

rew

0.09 0.058 0.063

Pie

rce

Hom

e B

rew

Com

pet

itor

S

Corn Leaves Arabidopsis Soybean Corn Kernel

Seed*

G r a s p t h e P r o t e o m e ®

Joint Annual Meeting of the American Society of Plant Biologists

and the Canadian Society of Plant Physiologists

Société Canadienne de Physiologie Végétale

Hynes Convention Center

Boston, MassachusettsAugust 5-9, 2006

For more information: American Society of Plant BiologistsTelephone: 301-251-0560 • Fax: 301-279-2996 • E-Mail: info@aspb.org • Web Site: http://www.aspb.org/meetings/pb-2006

Photo Credit: Greater Boston Convention & Visitors Bureau

SYMPOSIAPlants Mitigating Global Change—Stephen P. Long, University of Illinois

Legumes: Genomes to Biology—Douglas R. Cook, University of California-Davis

Ion Channels and Cellular Signaling—Julian I. Schroeder, University of California-San Diego

Gibbs Medal Symposium: Genome Scale BiologyJoseph R. Ecker, The Salk Institute

President's Symposium: Plant Responses to the EnvironmentMichael F. Thomashow, Michigan State University

Plan

t Bio

logy