peterson immortal life training

Life of the Mind 2011

Facilitator Training Presenter: Cynthia Peterson

Questions to consider…

What is the science of Cell Biology?

What was the breakthrough with HeLa cells?

What milestones in cell biology studies were possible because of HeLa cells?

How does one scientist react to this book?

What about research at the University of Tennessee?

Questions to consider…

What is the science of Cell Biology?

What was the breakthrough with HeLa cells?

What milestones in cell biology studies were possible because of HeLa cells?

How does one scientist react to this book?

What about research at the University of Tennessee?

What is the science of Cell Biology?

Work with cells in laboratory setting

Learn about cell structure, division, organization, normal and disease processes

Microscopy gives an up-close picture of the cells

Important step in drug development

What is the science of Cell Biology?

Work with cells in laboratory setting

Learn about cell structure, division, organization, normal and disease processes

Microscopy gives an up-close picture of the cells

Important step in drug development

What is the science of Cell Biology?

Questions to consider…

What is the science of Cell Biology?

What was the breakthrough with HeLa cells?

What milestones in cell biology studies were possible because of HeLa cells?

How does one scientist react to this book?

What about research at the University of Tennessee?

What was the breakthrough with HeLa Cells?

Human cells cultured for the first time! (Gey had cultured other species and had worked for over 20 years with this aim)

Cancer researchers considered cultured human cells to hold the key to discovering a cure

HeLa cells are a “workhorse” for cell biology: easy to grow, ideal for microscopy, transfection

Provided a model cell line for studying normal cellular processes as well as many diseases

Original Research on Polio Infection

Infected Cells

Uninfected Cells

Questions to consider…

What is the science of Cell Biology?

What was the breakthrough with HeLa cells?

What milestones in cell biology studies were possible because of HeLa cells?

How does one scientist react to this book?

What about research at the University of Tennessee?

What milestones in cell biology were possible because of HeLa cells?

Polio infection process and vaccine development

Advances in microscopy

Research to isolate single cells and eventually to establish clonal cell lines

Methods established for chromosome spreading and karyotyping

Ongoing research on telomerase (Nobel Prize 2009)

New siRNA technologies for gene knockdowns

What milestones in cell biology were possible because of HeLa cells?

Questions to consider…

What is the science of Cell Biology?

What was the breakthrough with HeLa cells?

What milestones in cell biology studies were possible because of HeLa cells?

How does one scientist react to this book?

What about research at the University of Tennessee?

How does one scientist react to this book?

Medicine and Science History of modern medicine

Doctor/Patient Relationship

Ethics, Values, and Civility Research Ethics

Legal Issues

How does one scientist react to this book?

Social, Economic and Political Aspects

Socioeconomic status

Race, class and gender

Research Journalism

Finding a passion

Questions to consider…

What is the science of Cell Biology?

What was the breakthrough with HeLa cells?

What milestones in cell biology studies were possible because of HeLa cells?

How does one scientist react to this book?

What about research at the University of Tennessee?

What about research at the University of Tennessee?

HeLa cells are used by many researchers across campus….. Where is a protein in the cell? What are its binding partners? How does localization change with cell cycle? Use transfection methods to introduce new genes into the

cell Use knockdown technologies to selectively reduce levels of

specific proteins

Examples from my own department Mobility of proteins in membrane Localize proteins within nuclear envelope Finding specific DNA/protein interactions

An example from an undergraduate researcher

Questions???