original cross of blue to wild santa isabel

Submission for new gene

Suggested gene name – Philippine BlueSuggested gene designation – pb/pb and +/pbNew Loci - yesGene type is recessive

Original cross of Blue to Wild Santa Isabel

Female Male

P1 Wild Type Santa Isabel +/+P2 Blue Albino Ghost S/+ - a/a - pb/pb

Expected percentage

F1 Ghost S/+ - +/pb - +/a 50%Silver +/pb - +/a 50%

Breed by Ken Kennedy

No counts and due to the gene being recessive the assumption is that all of the F1 offspring carry a single dose.

F1 mated pair from original cross

Female Ghost(S/+ -+/a - +/pb)

Male Silver(+/a - +/pb)

F1 Ghost S/+ - +/pb - +/aF1 Silver +/pb - +/a

Spawn Count 221

Phenotype GenotypeExpected

percentageExpected

countsActual count

F2 Blue Ghost S/+ - pb/pb 12.50% 28 26Blue Silver pb/pb 12.50% 28 21

0Silver +/pb 25% 55 90Ghost S/+ - +/pb 25% 55 84

0Silver +/+ 12.50% 28Ghost S/+ 12.50% 28

Albino No survivors

Breed by Damon Cartmell

Combined count of heterozygous pb and wild type due to recessive nature

Silver (+/+ -+/pb or +/+)

Heterozygous for blue gene or isn’t a carrier Blue Silver

(+/+ - pb/pb)

Homozygous for blue gene

F2

Silver Ghost(S/+ -+/pb or S/+ - +/+)

Heterozygous for blue gene or isn’t a carrier

Blue Ghost(S/+ - pb/pb)

F2

Blue Silver (+/+ - pb/pb) Silver

(+/+ -+/pb or +/+)

F2

Silver (+/+ -+/pb or +/+)

Heterozygous for blue gene or isn’t a carrier

Blue Silver (+/+ - pb/pb)

Homozygous for blue gene

F2

Blue Silver (+/+ - pb/pb)

F2

A top down view of the fish show how easily you can distinguish homozygous fish from the rest of the population

A light base color vs. tan.

F2

Phenotypic expression on known Genes/Alleles

Blue MarbleM/+ - pb/pb)

Blue ClownS/Z – pb/pb)

Blue ZebraZ/+ - pb/pb)

Platinum Marble(Gm/g – pb/pb)

Blue SmokeySm/+ - pb/pb)

Pinoy SmokeyD/+ - Sm/+ -pb/pb)

Pinoy ParaibaD/+ - S/S – pb/pb)

Pinoy ClownD/+ - S/Z – pb/pb)

Double Dark Pinoy Ghost(D/D – S/+ - pb/pb)

I would like to acknowledge the hard work that Mr. Ken Kennedy did in bringing the true nature of this gene to light

because without his contribution we may not be enjoying this beautiful new mutation.

Based on the above information I believe it is very obvious that this is a new mutation. It is a recessive gene on it’s own

Loci with no known Alleles at this time.

I ask at this time for The Angelfish Society to recognize and approve the name chosen by Mr. Kenneth Kennedy.

Philippine Blue (pb).