nutrient use by cyanobacteria in subtropical reservoirs
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Nutrient use by cyanobacteria in subtropical reservoirs
Michele Burford
Tim Davis, Priya Muhid, Matt Prentice Australian Rivers Institute
Stratified lakes & reservoirs promote cyanobacteria
Key questions
Should we invest in controlling nutrient inputs?
How do we decide whether nutrient inputs
are a problem? Nutrient concentrations Nutrient ratios Algal measures – chl, algal #, toxins
PN PN
PN
PN
PN PN
PN PN PN
PN 10 days supply
PN PN
1 day supply
Algal bioassays
N, P and N+P additions Photosynthetic yield
response (PHYTOPAM) after 24-48 h
Typically co-limited in
summer
Muhid & Burford, in press Inland Waters
Mesocosm experiments
Experimental design
Treatments Control Nitrate Urea Phosphate Nitrate + phosphate Urea + phosphate
Background nutrients
mg/L DIN + urea DIP DN:DIP
Expt 1 0.032 0.004 19:1 Expt 2 0.019 0.004 12:1
Biovolum
e (x10
7 µm
3 mL-‐1)
Biovolum
e (x10
7 µm
3 mL-‐1)
Ambient Control Nitrate Urea PhosphateNitrate + P Urea + P
Cyanobacteria Diatoms Chlorophytes CryptophytesChrysophytes Dinoflagellates Euglenophytes
Ambient Control Nitrate Urea Phosphate Nitrate + P Urea + P
Experiment 1 Experiment 2
3
2
1
0
3
2
1
0
T. Davis, unpubl. data
Urea + P stimulated phytoplankton
C. raciborskii response
0.0E+00
4.0E+06
8.0E+06
1.2E+07
1.6E+07
0.0E+00
4.0E+06
8.0E+06
1.2E+07
1.6E+07
AmbientT0
Ambient
Contro
l
Nitrate
Urea
Phosp
hate
Nitrate+
P
Urea+P
AmbientT0
Ambient
Control
Nitrate
Urea
Phosp
hate
Nitrate+
P
Urea+P
Cel
l bio
vol.
(€m
3 ml-1
) *
Expt 1
Expt 2
Expt 3
Cylindrospermopsins cell quota
0.00E+00
2.00E-07
4.00E-07
6.00E-07
8.00E-07
AmbientT
0
Ambient
Contro
l
Nitrate
Urea
Phosp
hate
Nitrate+
P
Urea+P
QTCYN
QdeoxyCYNQCYN A
A
A + B
A + B
BB B
Source: Google Earth
1
2
3
4
Upper reservoir zone
Mid reservoir zone
Lower reservoir zone
Source: Badin Gibbes
33P-phosphate uptake studies
Lake Wivenhoe, Queensland
Monthly sampling Four sites
Rapid P uptake
M. Prentice, unpubl. data
Threshold value
Uptake by starved C.raciborskii
0.0
0.5
1.0
1.5
2.0
2.5
3.0
0 5 10 15 20 25 30
Specific DIP up
take rate
[µg DIP µg Chl a
L-‐1hr
-‐1]
Days post T=0
P P
Active uptake
organicP
Slow use
Conclusions
Nitrogen and phosphorus promotes algal growth
Cyanobacteria have an active uptake mode
Measuring nutrient conc is of limited value
Acknowledgements Students & staff
– A.Chuang, S.Faggotter, C. Laurikaunen, C.Polson Collaborators
– Seqwater (P.Orr) – B.Neilan, J.Beardall, K.O’Brien, D. Hamilton Funding sources
Seqwater, Aust. Research Council, Griffith University
Species which grew
Expt 1 Expt 2 Expt 3
Monoraphidium Achnanthidium Synedra C. raciborskii Planktolyngbya
Achnanthidium Aulecoseira Synedra C. raciborskii Peridinium Cryptomonas
Aulecoseira C. raciborskii Planktolyngbya
Reservoirs in subtropical Australia
Tropic of Capricorn
Burford et al. 2012 Aquat Sci Burford et al. 2012 Aquat Sci
0.002
0.006
0.01
0.014
0.018
0
0.02
0.04
0.06
0.08
0.1
0.12
0.002
0.0025
0.003
0.0035
0.004
Jan-04 Jan-05 Jan-06 Jan-07 Jan-08 Jan-09
Ammonium
Nitrate + nitrite
Phosphate
Con
c (m
g L-1
)
Burford et al 2012
N+P increased chlorophyll a
0
20
40
60
80
0
20
40
60
80
Ambient
Contro
l
Contro
l
Nitrate
Urea
Phosp
hate
Nitrate+P
Urea+P
Chl
a (¬ g
L-1
)Expt 1
Expt 2
Background nutrients
mg/L DIN + urea DIP DIN:DIP Post floods 0.138 0.004 76:1 Expt 3 0.044 0.006 16:1
Post inflow event Biovolum
e (x10
7 µm
3 mL-‐1)
Ambient Control Nitrate Urea Phosphate Nitrate + P Urea + P
Cyanobacteria Diatoms Chlorophytes CryptophytesChrysophytes Dinoflagellates Euglenophytes
1.6
1.2
0.8
0.4
0
Experiment 3
Euphotic depth half previous experiments
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