modular pumps - waters corporation...waters quaternary gradient modules (qgm) are low-pressure...
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FLEXIBLE AND SCALABLE
The new series of Waters® innovative preparative pump technologies provide
extraordinary performance and convenience for benchtop purification of
microgram to multigram sample loads. As your lab’s productivity demands
increase, so do your expectations for reliability, performance, and flexibility of
your solvent delivery system. The full range of Binary and Quaternary Gradient
Modules have been designed to meet all of your purification needs.
The modules can be easily configured to perform a variety of purification protocols covering a wide range of sample matrices including
organic synthetics, natural products, peptides, and proteins. Exceptional flow rates provide you with the precision and accuracy you require,
delivering the chromatographic reproducibility that you need to fully automate all your laboratory’s processes. With exceptional solvent
blending, automated solvent management, and pulse-free delivery, Waters solvent delivery systems can meet even your most sensitive
application challenges.
Modular PumpsIncreased capability and versatility for preparative chromatography
Display Table of Retention Time % RSD
Injection # Peak 1 Peak 2 Peak 3 Peak 4
1 1.121 1.693 2.154 3.1212 1.124 1.697 2.159 3.122
3 1.118 1.689 2.150 3.1124 1.122 1.694 2.156 3.1225 1.121 1.693 2.153 3.1156 1.122 1.696 2.158 3.122Std Dev 0.002 0.003 0.003 0.004% RSD 0.17 0.17 0.16 0.14
QUAT ERNARY GRADIENT MODULES
Waters Quaternary Gradient Modules (QGM) are
low-pressure mixing quaternary gradient pumps
that serve as a solvent delivery device for a
variety of purification systems. Available in
three flow rate ranges: 0.5 to 50.0 mL/min,
0.5 to 150.0 mL/min, and 4.0 to 300.0 mL/min;
the modules simplify mobile phase delivery
management by accurately and precisely
mixing user-programmed eluent compositions.
Choose from three software options: MassLynx,®
Empower,® or stand-alone console software,
to conform to what is already being used in
your laboratory.
0.08
0.06
0.04
0.02
0.00
0.00 0.50 1.00 1.50 2.00 2.50 3.00Minutes
3.50 4.00 4.50 5.00 5.50 6.00
AU
Overlay of consecutive injections performed on the 2535 QGM, at 1 mL/min demonstrates the reproducibility that ensures accurate purification of consecutive samples and time based collections.
Isocratic separations with online blending of methanol and water illustrate the outstanding reproducibility of the 2535 QGM.
Empower-based purification system with the 2707 Autosampler, 2535 Quaternary Gradient Module and the 2489 UV/Visible Detector.
4
BINARY GRADIENT MODULES
Waters Binary Gradient Modules (BGM) incorporate extremely efficient preparative flow rates with greatly reduced system volumes,
capable of providing equivalent performance in both analytical and preparative modes.
Time
Time
Time
Time
-0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40 5.60 5.80 6.00 6.20 6.40 6.60 6.80 7.00 7.20 7.40 7.60 7.80 8.00
AU
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-0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40 5.60 5.80 6.00 6.20 6.40 6.60 6.80 7.00 7.20 7.40 7.60 7.80 8.00
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AU
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-0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40 5.60 5.80 6.00 6.20 6.40 6.60 6.80 7.00 7.20 7.40 7.60 7.80 8.00
AU
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5.0 4.6 mm column at 1.5 mL/min
10 mm column at 7.1 mL/min
19 mm column at 25.6 mL/min
30 mm column at 63.8 mL/min
0.43
0.28
0.11
0.73 2.221.58 3.34
4.264.83 6.07
3.84
3.82
0.523.242.09
4.244.46 6.124.84 5.68
3.85
0.48
3.292.130.65
4.24
4.43 6.104.83 5.62
3.870.43
0.28
0.11
0.73 2.221.58 3.34
4.264.83 6.07
* For more information, refer to Waters application note Analytical HPLC to Preparative HPLC: Scale-up Techniques Using a Natural Product Extract (720003120EN).
2545 BGM
The 2545 BGM is a high-pressure mixing binary gradient pump
that serves as the primary solvent delivery device for the
Waters AutoPurification™ System. The 2545 BGM provides
consistently smooth, pulse-free solvent flow for both analytical
and preparative scale flow rates under MassLynx Software
control with the FractionLynx™ Application Manager.
■■ Built on Waters proven high-pressure gradient
mixing technology
■■ Maintained pumping performance and flow rate range
■■ 0.5 to 150.0 mL/min
■■ Self priming from solvent container on floor
■■ Optional leak sensor
The broad flow rate range available with the 2545 BGM enables method development optimization on an analytical scale followed by preparative scale purifications, with no system modifications.* Scaled flow rates and injection volumes were calculated using the Waters OBD™ Calculator.
1525EF HPLC Pump
The 1525EF HPLC Pump is a high-pressure mixing binary
gradient pump that is utilized in many semi-preparative
configurations. Its exceptional flow rate provides you with
precision and accuracy, delivering the chromatographic
reproducibility your lab requires.
■■ 225 µL pump heads deliver up to 22.5 mL/min
■■ Optional FlexInject Manual Dual Injector Module for both
analytical and semi-preparative injections
■■ For semi-preparative applications: 10 to 30 mm I.D.
column separations
■■ Maximum operating pressure: 6000 psi up to 22.5 mL/min
1
2535 QGM ■■ Flow rate range from 0.5 to 50.0 mL/min, with separate
flow paths for analytical and preparative capability
■■ Programmable four-solvent selection
■■ Self priming from solvent container on floor
■■ Inlet manifold valve for large volume sample loading
■■ Maximum operating pressure: 6000 psi
2545 QGM■■ Flow rate range from 0.5 to 150.0 mL/min
■■ Programmable four-solvent selection
■■ Self priming from solvent container on floor
■■ Inlet manifold valve for large volume sample loading
■■ Maximum operating pressure: 6000 psi up to
100 mL/min, and 5000 psi at 150 mL/min
2555 QGM ■■ Flow rate range from 4 to 300 mL/min
■■ Programmable four-solvent selection
■■ Self priming from solvent container on floor
■■ Inlet manifold valve for large volume sample loading
■■ Maximum operating pressure: 3000 psi up to
200 mL/min, up to 2500 psi at 300 mL/min
AU
2.06
3.52
Time
This preparative separation of a pharmaceutical sample demonstrates the ability of the 2555 QGM to quickly and accurately resolve a large volume sample load.
Total load 500 mg Column: 50 x 50 mm SunFire™ C18, 10 µm
Conditions:Gradient from 10% A to 25% B over seven minutesSolvent A: 0.1% Formic acid in waterSolvent B: Acetonitrile
Flow rate: 250 mL/minSample: 0.2 g/mL Acetylsalicylic acid with ~0.02g/mL salicylic acidInjection vol: 2.5 mL (20 mL Loop)
The 2545 QGM with a manual inlet valve for loading large
volume samples.
Versatile injection capability
Solvent delivery systems can provide access to both analytical and
preparative scale injectors, with the Waters FlexInject Manual Dual
Injector Module mounted on the side of the unit, or the standalone
prep chromatography rack which can be located beside the pump.
To maximize the performance of the combination pump and dual
mode injector for both analytical and preparative flow rates,
the optical flow cells of the Waters 2489 TUV Detector and the
2998 PDA Detector are available in different formats. A dual
inlet, analytical and preparative flow cell, and a semi-prep flow
cell are available for both detectors. A variable pathlength flow
cell is available for the 2489 TUV Detector for high flow, large
concentration sample purifications.
For sample volumes that are too large for conventional injectors, the
QGMs include a manual, large volume inlet manifold. In addition,
automated, repetitive injections of sample solutions may be
introduced to the column through one of the four solvent lines, a
function that can be programmed through the instrument method.
*For more information, refer to Waters application note: A Modular Preparative HPLC System for the Isolation of Puerarin from Kudzu Root Extracts (720003797EN).
The separation of kudzu root extract using the 2707 Sample Manager (A). Very large injection volumes can be achieved by delivering sample through
a solvent line from the 2545 QGM (B) defined by the instrument method.*
Minutes
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AU
500 µL injection
Minutes
AU
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1.80
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2 mL of sample loaded
A
B
AUTOMAT ED SOLV ENT MANAGEMENT
Auto•Blend™ Technology enables you to generate
gradients automatically without having to manually
alter the buffer concentration, giving you the freedom
of using up to four solvents simultaneously. Auto•Blend
programming technology improves uptime, since no
premixing of mobile phases is required. You can build
both column cleanup and system flushing into your
methods. In addition to method development, Auto•Blend
simplifies maintenance by offering straightforward eluent
management, increasing the flexibility and efficiency of
your laboratory.
5%
90%
5%
0%
A
B
C
D
Water
Acetonitrile
100 mMAmmonium
Formate
100 mMAmmoniumBicarbonate
Initial Conditions Final Conditions
Initial Final
90%
5%
5%
0%
5 mM
buffer
5 mM
buffer
SOF T WARE CONT ROL
Integrated system monitoring and diagnostic software provided
with the instrument enables operators to monitor several key
parameters including solvent usage, system pressure, and
gradient composition. Interactive control capabilities provide
unique diagnostic tools with visual indicators allowing for easy
troubleshooting and visualization of both the pump and the
UV detectors’ current status.
Console control of the solvent delivery system and the detector for a simplified solution with user diagnostics and independent instrument control.
Channel A and organic composition change are monitored, and the information collected, which can then be reported in a simple JPEG or BMP format.
With several data channels to choose from, users can easily
configure the system to monitor the data channels they require;
such as gradient composition, flow rate, and detector absorbance.
Fully integrated control of Waters Quaternary Gradient Modules
(QGMs) with autosamplers, detectors, and fraction collectors
is available through Empower Software, our industry-leading
chromatography data software (CDS) platform, or MassLynx
Software.
The Waters FlexInject Manual Dual Injector Module is available with any of the Waters preparative pumps, and
provides easy access to both analytical and preparative scale injections.
For CDS independent control of both the QGM and the 2489 TUV
Detector, the instrument console provides complete access to
method creation and a sample list format for system use with
software independent injectors and collectors.
The instrument console provides an interactive diagnostic tool for easy visualization of the overall pump status.
Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com
Waters, T he Science of What’s Possible, MassLynx, and Empower are registered trademarks of Waters Corporation. FractionLynx, SunFire, OBD, Auto•Blend, and AutoPurification are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.
©2011 Waters Corporation. Printed in the U.S.A. July 2016 720003897EN LM-SIG
ORDERING INFORMATION
Quaternary Gradient Modules2535 QGM – Flow rate range 0.5–50.0 mL/min 1760017392545 QGM – Flow rate range 0.5–150.0 mL/min 1760017362555 QGM – Flow rate range 4.0–300.0 mL/min 176001741
Prep Chromatography Rack 205000619
Binary Gradient Modules1525 Binary Gradient Pump 0–10 mL/min 1860015251525 EF Upgrade Kit, for flow rate to 22.5 mL/min 2050003242545 BGM – Flow rate range 0.5–150.0 mL/min 186025450
BUILD A P REPARAT IV E CHROMATOGRAPHY SYST EM TO MATCH YOUR NEEDS, WORKLOAD, AND BUDGET
Choose from a variety of high performance components to configure a preparative chromatography system that’s right for your lab.
Whether your application requires low or high pressure mixing, low or high flow rates, manual or automated injection and collection,
you can choose the exact level of functionality and capacity that your application requires. And since Waters’ purification systems are
upgradable, you don’t have to worry about your investment as your workload increases.
Sample Load Column I.D. Fluid Handling Unit Max Flow Rate
µg – mg 3.9 – 7.8 mm 1525 Binary HPLC Pump* 10 mL/min
µg –10s mg 3.9 – 19 mm 1525 Binary HPLC Pump* and EF Kit 22.5 mL/min
mg –10s mg 3.9 – 50 mm 2535 Quaternary Gradient Module 50 mL/min
m – g 4.6 – 50 mm 2545 Binary Gradient Module* 150 mL/min
m – g 4.6 – 50 mm 2545 Quaternary Gradient Module 150 mL/min
mg – 10s g 7.8 – 75 mm 2555 Quaternary Gradient Module 300 mL/min
* High pressure mixing
Choose a solvent delivery system based on the amount of material you will be loading on the column and the size of the column.
2545 BGM.
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