microbiology presentation - rich boehler
Post on 23-Jan-2017
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The Journey of a Scientist
Thank you for the chance to share “science”.
Mr. Boehler
Microbiology: The science of microorganisms (bacteria and fungi)
• Biology – Microbiology– Immunology
• Chemistry• Physics
Summary – What are microbes?
– cell
– Laboratory Testing with technology.
– Microscopic examination
– Statistics
– Sample Science Data
- Lab Safety - Group Exercise – with skittles
Simple to Complex Life
Human Body Biology • Humans• Animals• Plants• Mold• Yeast• Bacteria• Viruses
Understanding how cells function provides insight into solving challenging problems
Bacteria Cells:Help DigestionNatural Balance
Human CellsTissues
Brain, Kidneys, LiverPancreas, Stomach, Heart, Skin, Bones
Technology
Nature: glowing fire-flyLaboratory
Applying the “glowing” mechanismto the microbiology laboratoryto check products for Quality.
Essential Tools for Young Scientists:
Scientific MethodA. Literature Review =
LIBRARY! B. HypothesisC. Method D. Materials
E. ResultsF. DiscussionG. Conclusion
• Think!• Read• Write!
• Publish• Present and Prepare to Be Challenged
• Apply learned science in a practical / helpful way.
– Some Examples = • Environment• Product Safety• Human Health• Animal Health
Environmental MicrobiologyWhere do bacteria, mold –& yeast come from?
Plant Microbes
Soil Microbes
Water Microbes
Air Microbes
Surface Microbes
Petri Dish containing microbes
Soil Microbes
• Bacillus bacteria
Skin Microbes
Bacteria from contaminated water
Gram negative rodsE. coli
• What does this mean?– Although a lot of bacteria out
there are good… there are some that contain harmful parts that can make us sick…
– That is why it is important to always wash hands for an ample amount of time, using soap.
– Cook food well, store food properly and be aware of expiration dates.
StaphE. coli
MoldNegative Control Yeast
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
Microorganism ATP in Lotions, Sunscreens, Gels & Face/Eye
Serums
Mea
sure
d Li
ght (
log)
Sample Data: how to read aGraph with science results..?
• Create Summary Data graph.
• Tool for data analysis process.
Microscope • Why do we need a
microscope?
• Pathogenic vs. non-pathogenic bacteria
Some of the things we see in a microscope
Staph aureus Bacillus cereus
Mold – yuck; is this mold bad?
Yeast
Why are fungi so important?
Penicillin medicine Bread rising• The essentials of any bread dough are
flour, water, and yeast.
• The yeast metabolizes these simple sugars and exudes a liquid that releases carbon dioxide and ethyl alcohol into existing air bubbles in the dough.
Safe Products
Lab Safety
Protective Personal Equipment
• Safety goggles/glasses
• What should this lab technician be wearing?
Laboratory Measurements
Measurements• Graduated Cylinder• Beakers
– Why are accurate measurements important in the lab?
• Formulation of Media
Quantitative Research Experiment
• Candy – count the number of pieces of candy you have.
• Each piece of candy = a colony of bacteria.
• Separate the candy (skittles) into groups based on the color.
• On paper, write the number of candies for each color.
– Graph the data collected.
• Red = Yeast (rhodotorula)• Yellow = skin bacteria (staph)• Orange = bacillus bacteria• Green = Mold
• Y – axis = number of candy pieces (number of microorganisms)
• X – axis = type of microorganism
Collecting and Summarizing DataI. Build a Table
Color Number of pieces
Type of microorganism
Red 10 Yeast
Green 4 Mold
Yellow 3 Staph
Orange 11 Bacillus
Purple 1 E. coli
Blue 6 Listeria
II. Build a Graph
Yeast Mold Staph Bacillus E. coli Listeria0
2
4
6
8
10
12
Type and Frequency of Microorganisms present on school desks
Micr
oorg
anism
Col
onie
s
Conclusion• As manager of the Ambix microbiology lab:
– Test products for market release.– Document and communicate results.
– Summarize results using statistics and the scientific method.
– Maintain an environmental monitoring program.– Maintain a water quality program.
– Interface with FDA.
– Investigate microbiology related challenges.– Validate microbiological methods.– Write and revise Procedures to keep current.
– Research and Development
• Science is an amazing field to be in!– There are many areas of biology.
– I pursued and continue to be challenged in microbiology with a goal to ensure that products are safe.• The goal is attainable and successful
with the application of knowledge and experience – Bachelor of Science Degree – Biology (Stony Brook University; 1999)– Master of Science – Microbiology /
Immunology (Long Island University; 2002)
– Experience – learned from mentors; training; U.S. Navy Reserves and the pursuit of scientific challenges.
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