micro lab
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12/29/2012
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Sessions: • Gram stain and Bacteriology
• Bacillus, diphteroids,staph,and strept under the microscope
• Medias
• Sensitivity test
• Normal flora bacteria and throat culture
• Urine and stool culture
• Agglutination and precipitation tests
• Acid fast culture and stain, and yeast, candida, and Aspergillus.
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Gram stain and Bacteriology Bacillus, diphteroids, staph, and strept under the
microscope
Streptococcus pneumoniae gram postive ,diplococci, lancet shape
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Diphteroids Gram positive , Chinese letters ( rods)
Bacillus spp. Gram positive , rods, there are spores
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Blood agar for –ve and +ve bacteria
Contains blood cells from an animal (e.g. a sheep); most bacteria will grow on this medium , but it is NOT Suitable for Student Use due to potential for contamination from human contact. Btw….. it so advantageous for distinguishing the pathogenic and the normal bacteria by noticing the Decomposition RBC which indicate to the pathogenic bacteria.
Alpha-type Hemolytic Streptococcus it turns into green.. alpha hemolytic >green beta hemolytic > yellow gamma hemolytic > no hemolysis
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Beta Hemolytic Bacteria it turns to yellow
The picture on the left is group A Beta hemolytic which is sensitive to Bacitracin and The picture on the right is group B Beta hemolytic which is resistanat to Bacitracin.
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MacConky Agar for some -ve
a culture medium designed to grow Gram-negative bacteria so it's a selective media** and we can called it indicator media **, also it is used for distinguishing between lactose fermental bacteria which appears pink in color and lactose none-fermental bacteria that is appears yellow in the same media .
CLED agar a selective media for the gram positive & it is used for differentiations and enumeration of microorganisms in urine and to distinguish between Staphylococcus aureus which produces green colonies on this medium and the staphulococcus intermedius which produces yellow colonies on the same medium .
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Hecton Enteric agar
Hecton enteric agar media it’s selective for salmonella and shegella , its color is yellowish to orange salmonella growth turns the color into blue shigella growth turns its color into green
TCBS
TCBS :Thiosulfate-citrate-bile salts-sucrose agar or TCBS agar is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio spp. TCBS Agar is highly selective for the isolation of Vibrio cholera
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kligler’s iron agar… is red in color, solid put these test tubes in the autoclave to make sure they are sterile after that we should tilt them and till the agar gets solid. SIM agar… we use this colorless media to show three things:H2S production ,, indole +/- and motility
Kigller’s agar Citrate +ve>> blue -ve >>green
Tubidity test (Motility)
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Sensitivity test
A plate containing Alpha hemolysis streptococcus viridans which is resistance to the antibiotic Optochin
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Staphylococcus Aureus culture on CLED agar at the bottom ( yellow) Staphylococcus epidermidis culture on CLED agar at the bottom ( white)
Staphylococcus Aureus culture on CLED agar ( yellow)
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Staphylococcus epidermidis culture on CLED agar ( white)
MSA (MANNITOL SALT AGAR): is a selective media for Gram positive staphylococci & Micrococcaceae The original color of the media is pink/red. - Staphylococcus Aureus (Coagulase +ve) turns the color to yellow -Staphylococcus Epidermidis (Coagulase -ve ) the color remains pink
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Klebsialla on Blood
Klebsialla is non-motile , weak fructose fermenter, and its colonies have a very mucoid appearance and that because this bacteria has a capsule
Klebs on MacConky
Klebsialla
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Stool Culture
Salmonella
a growth of salmonella with a lot of H2S; (black dots inside the colony)
Hikton Enteric agar
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In blood culture , it is highly motile , its make wavy appearance (the wavy appearance on media is called swarming phenomena).It has a bad smell.
TCBS media ,its color is green , if there is growth for vibrio ,the plate color is turned from green to golden(the color is changed
due to sucrose fermentation ).
Vibrio Cholera
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pseudomonas on cled has metalic appearence +silver color
Pseudomonas it has a good smell, its color is silver
Shigella E coli H2S Production
SIM agar The 1st one on the right >> H2S production the 2nd one >> Indole positive
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Agglutination and precipitation tests
counter-immuno electrophoresis(Qualitative method)
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Rocket electrophoresis(both qualitative & quantitative)
Double immune-diffusion technique(Qualitative method)
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Antibody antibiotic reaction
Acid fast culture and stain, and yeast, candida, and Aspergillus.
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