medicinal and pharmaceutical applications of dna technology

DIZONMERCADO

ORTIZSACLAUSO

JIMENEZ

IV - Gluon

A little game :3

Three volunteers get a paper each and do what’s written in it in the same order. 1 min,1 act. Same-numbered acts are simultaneous.

The rest, make 2 groups. Volunteers face and act for the right group

and codes on their backs for the left group to decode “disease”.

Compare and check answers.

Lahat mahilig kumain. Si Gab lang ang nagjajapanese at

nangangati. Malaki bag ni Shir. Madalas tumakbo si Shir at Gab. Sumisigaw si Zoe pag galit. Sumasabay si Zoe sa pagsayaw at pagkanta

ni Shir. English ispikining si Gab at Zoe kaya

madalas mag-usap.

Right Side Actor/Actress Left Side

Currently a still experimental and widely-explored technology

Most basic concepts are PCR and the HGP Has multiple uses:

◦ Microbe identification◦ Determination of inheritable diseases◦ Gene therapy◦ Cloning (Experimental)

Specifically microbial diseases Two identification methods

◦ Phenotyping (Traditional Microbial Diagnosis)◦ Genotyping (As stated by word)

Check for the following:◦ Morphology◦ Staining properties◦ Reaction to various stimuli◦ Other phenotyping methods/protocols

Disadvantages◦ Hard to reproduce◦ Hard to make exact identification◦ Technical difficulties in typing

Identification up to nucleic acid level Method:

1. Get sample2. Check for marker.3. Perform PCR.4. Compare with previously identified samples.

Easier identification

Used to predict hereditary diseases even before onset of birth

Three methods for testing:◦ Identification:

Amniocentesis Chorionic villus sampling (CVS)

◦ Specification

Amniocentesis: extract fetal cells from amniotic fluid

CVS: extract placental cells Culture analysis:

◦ Chromosomes◦ Proteins◦ Enzymatic reactions◦ Etc.

Both for determination of what causes the trait on a CHROMOSOMAL LEVEL

Used for determining what PART OF THE GENE (base pair, etc.) caused the expression of the trait.

Process:1. Remove target gene2. Replicate gene3. Compare to previously determined normal gene

Used for treatment of genetic diseases Uses the Human Genome Project as the

ultimate basis Vectors are used as containers of

inoculation◦ Retrovirus◦ Adenovirus◦ Liposome◦ (Or in some cases, none)

1. Take cell from patient2. Change extract DNA’s defective parts into

non-defective (gene splicing)3. Insert transformed DNA into virus4. Virus infects another cell of patient5. Let infected cell multiply6. Insert back to patient

Artificial creation of proteins (usually enzymes) for people who are incapable of producing their own.

E.g. Insulin, anti-venom Method:

1. Get protein-coding gene2. Insert to bacterium3. Make it produce target protein4. Harvest.

In production◦ Stability of replication◦ Viability of samples◦ Effectiveness of treatment

Ethics◦ New fields such as tissue and human cloning

1. Give a use of DNA Medical Technology.2. There are three disadvantages to phenotypic

microbial identification. Name them ALL. (3 pts.)3. Give the general process of one method of pre-

natal prediction.4. Give the general process for gene therapy.5. Give a category of protein that can be artificially

created (not counting insulin).6. Why is gene therapy sometimes ineffective if not

detrimental?7. Why is human cloning currently unethical (given

our current methods)?8. What is the central method behind DNA

amplification?

DECODE THIS :D

What programming language did I use to make the answer key to the UBER-BONUS? (Hint: It’s not Java)

What do you call my [Gab] hairstyle? (Hint: No vanity intended.)

How many slides are there here? (Hint: Lol) Shir’s full name? (w/MIDDLE NAME)

Sinong 2nd Year ang madalas mangulit kay Isko? [Hint: HE SO TALL]