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Lonza Agarose
SeaKem®, NuSieve®, MetaPhor®
Agarose Selection and Preparation
Lonza Agarose brings consistency and reliability to your most fundamental research techniques. Selecting the best agarose for your application will minimize errors, optimize results and even reduce costs. Lonza offers a range of agarose types designed by fragment size, sample type and application. Our agarose is engineered for high performance in resolution, blotting and recovery, while providing the best gel strength for easy handling
The selection tools below will help you select and prepare the best agarose gels for your application. For more information, visit www.lonza.com/sourcebook to view our on-line Sourcebook for Electrophoresis.
Agarose and compatible techniques
Recovery MethodSeaKem®
LESeaKem®
GTG®SeaPlaque® SeaPlaque®
GTG®NuSieve®
3:1NuSieve®
GTG®MetaPhor® SeaKem®
GoldSeaPrep® InCert® I.D. NA®
In-Gel Reactions ■ ■
ß-Agarase ■ ■ ■
Phenol/Chloroform ■ ■ ■
Recovery Columns ■ ■ ■ ■ ■ ■ ■ ■ ■
Electroelution ■ ■ ■ ■ ■ ■ ■ ■ ■
Freeze/Squeeze ■ ■ ■ ■ ■ ■ ■ ■ ■
BlottingSouthern <1 kb ■
Southern >1 kb ■
Northern <1 kb ■
Northern >1 kb ■
Specialty Applications
Viral Plaque Assays ■ ■
Preparation of Megabase Samples ■
PFGE ■ ■
Cell Culture ■ ■ ■
Encapsulation & Embedding of Cells ■
DNA Identity Testing ■
Comet Assays ■ ■
Low MeltingTemperature
Low MeltingTemperature
Low MeltingTemperature
Low MeltingTemperature
0 400 bp 1,000 bp 25,000 bp
Performance Certi�edGenetic Technology Grade™High Gel Strength
Low MeltingTemperature
10,000,000 bp
20 bp - 800 bp
50 bp - 1 kb
50 bp - 1 kb
200 bp - 25 kb
100 bp - 23 kb
100 bp - 23 kb
200 bp - 25 kb
1 kb - 10 Mb
200 bp 600 bp 800 bp
Agar
ose
and
Appl
icat
ions
Fragment Size Range
SeaKem® Gold Agarose
SeaPlaque® GTG® Agarose
SeaKem® GTG® Agarose
NuSieve® GTG® Agarose
NuSieve® 3:1 Agarose
MetaPhor® Agarose
SeaKem® LE Agarose
SeaPlaque® Agarose
GTG
GTG
GTG
GTG
GTG
Choose the Agarose that is right for you
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Agarose Selection and Preparation
3
SeaKem® GTG®
A B
SeaPlaque® GTG®
A B
2,500 bp –
400 bp –
– 23,130 bp– 4,361 bp
Separation of DNA markers in 1% SeaKem® GTG® and SeaPlaque® GTG® Agarose gels in 1X TBE Buffer (Prepared from AccuGENE® 10X TBE Buffer). Lane A: Lonza's 50 - 2,500 bp DNA Marker (~25 ng/band)Lane B: New England BioLabs Hind III digest of lambda DNA (0.125 mg/lane)
Performance of Lonza Agarose for DNA >1kb
Final Agarose Concentration % (w/v)
Size Range (Base Pairs) 1X TAE Buffer 1X TBE BufferSeaKem® LE and SeaKem® GTG® Agarose1,000 – 23,000 0.60 0.50800 – 10,000 0.80 0.70400 – 8,000 1.00 0.85300 – 7,000 1.20 1.00200 – 4,000 1.50 1.25100 – 3,000 2.00 1.75NuSieve® 3:1 Agarose500 – 1,000 3.0 2.0100 – 500 4.0 3.010 – 100 6.0 5.0MetaPhor® Agarose150 – 800 2.0 1.8100 – 600 3.0 2.050 – 250 4.0 3.020 – 130 5.0 4.0<80 — 5.0SeaPlaque® and SeaPlaque® GTG® Agarose500 – 25,000 0.75 0.70300 – 20,000 1.00 0.85200 – 12,000 1.25 1.00150 – 6,000 1.50 1.25100 – 3,000 1.75 1.5050 – 2,000 2.00 1.75NuSieve® GTG® Agarose500 – 1,000 2.5 2.0150 – 700 3.0 2.5100 – 450 3.5 3.070 – 300 4.0 3.510 – 100 4.5 4.08 – 50 5.0 4.5SeaKem® Gold Agarose†
5,000 – 50,000 0.3 —1,000 – 20,000 0.5 —800 – 10,000 0.8 —400 – 8,000 1.0 —
† TBE Buffer is not recommended for separation of DNA >12,000 bp.
Buffer Suggested Uses and CommentsTAE Buffer Use when DNA is to be recovered
Use for electrophoresis of large (>12 kb) DNALow ionic strengthLow buffering capacity – recirculation may be necessary for extended electrophoretic times
TBE Buffer Use for electrophoresis of small (<1 kb) DNADecreased DNA mobilityHigh ionic strength High buffering capacity – no recirculation required for extended run times
Lonza types are listed as FDA Class 1 Medical Devices (#1219614)Our agarose is GUARANTEED DNase/RNase Free
– 622 bp
– 238 bp– 242 bp
NuSieve® 3:1 A B
NuSieve® GTG® A B
MetaPhor® A B
1,000 bp –
Separation of DNA markers in 3% NuSieve® 3:1, NuSieve® GTG® and MetaPhor® Agarose gels in 1X TBE (Prepared from AccuGENE® 10X TBE Buffer).Lane A: Lonza’s 50 - 1,000 bp DNA Marker (~25 ng/band)Lane B: New England BioLabs Msp I digest of pBR322 (0.125 mg/lane)
Performance of Lonza Agarose for DNA <1 kb
Resolution properties of Lonza Agarose
Suggested agarose concentrations and buffer
3.00 A B
4.00 A B
1X TBE Buffer
Separation of DNA markers in 0.75% to 1.25% SeaKem® GTG® Agarose gels in 1X TAE Buffer. 1 kb DNA ladder; 1 µg/lane.
Separation of DNA fragments in 2%, 3%, and 4% NuSieve® 3:1 Agarose gel in 1X TBE Buffer.Lane A: Lonza’s 100 bp Extended Range DNA Ladder (4 - 7.5 ng/band) Lane B: Invitrogen, Inc.’s Hae III digest of φX174 (0.25 ng/band)
12 kb –
3 kb –
1 kb –
0.5 kb –
1X TAE Buffer % 0.75 1.00 1.25
Impact of buffer on resolution
% 2.00 A B
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Routine Separation and Blotting–Small Fragments
STRENGTH
HIGH GELNuSieve® 3:1 Agarose Fine resolution of small fragments
■ Fine resolution of small DNA fragments ■ Resolves a 4% - 6% difference in fragments 50 bp to 1 kb■ High gel strength for blotting■ Ideal for RT-PCR and genotyping
Ordering Information
Cat. No. Size
50091 25 g
50090 125 g
50094 500 g
50095 1 kg
Recommended standardsCat. No.50330 20 – 500 bp DNA Ladder50461 50 – 1,000 bp DNA Marker50334 100 – 1,000 bp DNA QuantLadder
MetaPhor® Agarose Ultra fine resolution of small fragments
■ Utra fine resolution–detects 2% size difference in fragments 20 bp to 800 bp■ Rivals the resolution of polyacrylamide■ Ideal for small PCR and STR analysis
Ordering Information
Recommended standardsCat. No.50330 20 – 500 bp DNA Ladder50320 20 – 1,000 bp Extended Range DNA Ladder50461 50 – 1,000 bp DNA Marker
Cat. No. Size
50181 25 g
50180 125 g
50184 500 g
50185 1 kg
A 550 bp sequence from lambda DNA was amplified (25 cycles) using primers and Taq DNA polymerase supplied in the GeneAmp® Kit (Roche Molecular Systems). PCR products and controls were electro phoresed on a 4% NuSieve® 3:1 Agarose gel in TAE Buffer at 5 V/cm for 3 hours. Lane 1, Msp I digest of pBR322 DNA (1.5 µg); lane 2, Hae III digest of øX174 DNA (1.5 µg); lane 3, no DNA control; lanes 4 – 9, PCR products resulting from different reaction conditions (7 µl of 100 µl reaction mixture); and lane 10, a positive control where kit template was added.
1 2 3 4 5 6 7 8 9 10
PCR Product
Primer-Dimer
Primer
1,353 bp –
622 bp –
404 bp –
242 bp –
238 bp –
67 bp –
34 bp –
NuSieve® 3:1 Agarose gel for PCR products
8% Polyacrylamide vertical format 8 bp Msp I 4 bp Ladder Digest Ladder
DNA ladders with 4 bp or 8 bp step sizes were prepared by ligation of Bgl II linkers. Aliquots of 0.8 µg of the ladders were separated on a 3.5% MetaPhor® Agarose gel in a horizontal format and compared to an 8% polyacrylamide gel run in a vertical format in TBE Buffer. The horizontal gel (15 cm × 20 cm and 3.0 mm thick) was run at 6.7 V/cm for 4 hours at 15°C. The vertical gel (10 cm × 20 cm and 1.0 mm thick) was run at 8 V/cm for 2 hours.
– 67 bp
– 34 bp
3.5% MetaPhor® Gel horizontal format 8 bp Msp I 4 bp Ladder Digest Ladder
622 bp –
242 bp –
238 bp –
64 bp –56 bp –
32 bp –
High resolution of short DNA ladders in MetaPhor® Agarose
0 400 bp 1,000 bp 25,000 bp 10,000,000 bp200 bp 600 bp 800 bp
0 400 bp 1,000 bp200 bp 600 bp 800 bp
0 400 bp 1,000 bp 25,000 bp200 bp 600 bp 800 bp
Fragment size range
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Routine Separation and Blotting–Large Fragments
STRENGTH
HIGH GELSeaKem® LE Agarose Routine analysis and blotting
■ Broad range, multipurpose for fragments 100 bp to 23 kb■ Optimized for PCR fragments greater than 1 kb■ High gel strength for Southern and Northern blotting■ Easy to dissolve
1 2 3 4
– 3,000 bp
– 1,000 bp
1% SeaKem® LE Agarose gel
Recommended standardsCat. No.50321 100 – 1,000 bp DNA Ladder 50631 50 – 2,500 bp DNA Marker50323 500 – 8,000 bp DNA Ladder
Ordering Information
Cat. No. Size
50001 25 g
50002 100 g
50000 125 g
50004 500 g
50005 1 kg
Various Lonza markers separated in 0.3% and 0.5% SeaKem® Gold Agarose gels in 1X TAE Buffer. Lanes 1&3: 1 kb ladders. Lanes 2&4 markers (8.3, 8.6, 10.1, 12.2, 15.0, 17.0, 19.4, 22.6, 24.8, 29. 9, 33.5, 38.4, 48.5 kb). Gels were run at 1 V/cm for 16 hours.
48 kb –
19 kb –
12 kb –
6 kb –
4 kb –
2 kb –
1 kb –0.5 kb –
0.3% 0.3% 0.5% 0.5%
Resolution performance of SeaKem® Gold Agarose
1 2 3 4
Lambda DNA Ladders and S.cerevisiae DNA Standards were run on the Bio-Rad® CHEF-DR® III System.
Lambda Ladders
Ordering Information
Cat. No. Size
SeaKem® Gold® Agarose50152 25 g
50150 125 g
50121 1 g
50123 5 g
InCert® Agarose
STRENGTH
HIGH GEL
CERTIFIED
PERFORMANCE GTGSeaKem® Gold Agarose Megabase DNA and PFGE
■ Rapid separation of large DNA 30 kb to 50 kb by horizontal electrophoresis ■ Separation of DNA 50 kb to 10 Mb by pulsed field gel electrophoresis■ Good multipurpose separation of fragments greater than 1 kb■ Very strong, easy to handle gels at low concentrations■ Use InCert® Agarose for preparation of chromosomal DNA prior to PFGE
Recommended standardsLambda DNA Ladder50 Kb to 873 Kb Cat. No. Size Pg DNA/plug50401 5 plugs 10 ± 2
Saccharomyces cerevisiae DNA Standard220 kb≈1Mb Chromosomal DNA50411 5 plugs 5 ± 2
STRENGTH
HIGH GEL
CERTIFIED
PERFORMANCE GTGSeaKem® GTG® Agarose Performance certified multipurpose
■ Multipurpose electrophoresis and cloning of fragments 100 bp to 23 kb■ Ideal for recovery by electroelution■ High gel strength for Southern and Northern blotting■ Performance tested and certified for digestion and ligation
Ordering Information
Cat. No. Size
50071 25 g
50070 125 g
50074 500 g
Recommended standardsCat. No.50461 50 – 1,000 bp DNA Marker50321 100 – 1,000 bp DNA Ladder50334 100 – 1,000 bp DNA QuantLadder
0 400 bp 1,000 bp 25,000 bp 10,000,000 bp200 bp 600 bp 800 bp
0 400 bp 1,000 bp200 bp 600 bp 800 bp
0 400 bp 1,000 bp 25,000 bp200 bp 600 bp 800 bp
Fragment size range
Ladders (Lonza)1. 1 – 10 kb Marker2. 50 – 2,500 bp Marker3. 500 bp Ladder4. 100 bp Extended Range DNA Ladder
5
S. cerevisiae
8
0 400 bp 1,000 bp 25,000 bp 10,000,000 bp200 bp 600 bp 800 bp
0 400 bp 1,000 bp200 bp 600 bp 800 bp
0 400 bp 1,000 bp 25,000 bp200 bp 600 bp 800 bp
Fragment size range
Recovery and In-Gel Reactions–Small Fragments
Ordering Information
Recommended standardsCat. No.50461 50 – 1,000 bp DNA Marker50334 100 – 1,000 bp DNA QuantLadder50335 100 – 1,000 bp Reverse QuantLadder
Cat. No. Size
NuSieve® GTG® Agarose50081 25 g
50080 125 g
50084
50085
500 g
1 kg
b-Agarase58001 100 units
58005 500 units
DNA fragments were separated in 2%, 3%, and 4% NuSieve® GTG® Agarose gels in 1X TBE Buffer. Lane A: Hae III digest of øX174 DNA, 0.5 µg/lane. Lane B: Msp I digest of pBR322 DNA, 0.5 µg/lane.
3% A B
4% A B
– 622 bp
– 242 + 238 bp
– 160 bp– 147 bp
– 90 bp
– 67 bp
– 34 bp
2% A B
1,353 bp –
603 bp –
281 + 271 bp –
194 bp –
118 bp –
72 bp –
Fine resolution of low molecular weight DNA fragments in NuSieve® GTG® Agarose
NuSieve® GTG® Agarose Performance certified for small fragment recovery and in-gel reactions
■ High resolving low melting temperature agarose ■ Resolves a 4% difference in fragments 50 bp to 1 kb■ Performance tested and certified for digestion and ligation ■ Use β-Agarase for quantitative recovery of DNA and RNA
MELTING
LOw
CERTIFIED
PERFORMANCE GTG
Low melt and Genetic Technology GradeTM Agarose Agarose is one of the most important factors in DNA recovery. Lonza offers low melting temperature agarose specifically engineered for a range of recovery methods.
For recovery without purification, use an agarose designed and tested for in-gel reactions (nucleic acid reactions in the presence of re-melted agarose, such as in-gel cloning, in-gel PCR and in-gel sequencing).
Genetic Technology GradeTM (GTG®) Agarose is manufactured, tested and certified for performance in downstream enzymatic applications. The additional GTG® testing provides a more reliable index of product quality, and ensures the agarose will yield biologically active DNA. Each lot of Lonza GTG® Certified Agarose is tested for the following:■ DNA binding■ DNase and RNase activity■ DNA resolution and gel background ■ In-gel cloning and restriction digestion (low melt agarose)■ Restriction ligation assay (SeaKem® GTG® Agarose)
Performance Certi�edGenetic Technology Grade™
GTGLonza GTG® Certified Agarose:■ SeaKem® GTG® Agarose■ SeaPlaque® GTG® Agarose ■ NuSieve® GTG® Agarose■ SeaKem® Gold Agarose
Other Lonza Agarose is Molecular Biology Grade and is screened for specific analytical characteristics, as well as DNA binding, DNase and RNase activity, and gel background.
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Endotoxin Testing
0 400 bp 1,000 bp 25,000 bp 10,000,000 bp200 bp 600 bp 800 bp
0 400 bp 1,000 bp200 bp 600 bp 800 bp
0 400 bp 1,000 bp 25,000 bp200 bp 600 bp 800 bp
Fragment size range
Recovery and In-Gel Reactions–Large Fragments
SeaPlaque® Agarose Low melting temperature for recovery
■ Recovery and cloning of fragments 200 bp to 25 kb■ Ideal for cloning tissue culture cells■ Optimized for viral plaque assays■ Use β-Agarase for quantitative recovery of DNA and RNA
MELTING
LOw
SeaPlaque® GTG® Agarose Performance certified for large fragment recovery and in-gel reactions
■ Broad separation and recovery of fragments 200 bp to 25 kb■ Performance tested and certified for digestion and ligation without additional purification■ Use β-Agarase for quantitative recovery of DNA and RNA
MELTING
LOw
CERTIFIED
PERFORMANCE GTG
10 kb –
2 kb –
1 kb –
– 3 kb
– 1.3 kb
– 700 bp
– 400 bp
– 100 bp
1% SeaPlaque® Agarose gel
1 2 3 4
Ladders (Lonza)1. 1 – 10 kb Marker2. 50 – 2,500 bp Marker3. 500 bp Ladder4. 100 bp Extended Range DNA Ladder
Ordering Information
Cat. No. Size
SeaPlaque® GTG® Agarose50111 25 g
50110
50115 1 kg
125 g
b-Agarase58001 100 units
58005 500 units
Recommended standardsCat. No.50323 500 – 8,000 bp DNA Ladder50471 1 – 10 kb DNA Marker50334 100 – 1,000 bp DNA QuantLadder
Ordering Information
Cat. No. Size
SeaPlaque® Agarose50101 25 g
50100 125 g
50105 1 kg
b-Agarase58001 100 units
58005 500 units
Recommended standardsCat. No.50321 100 – 1,000 bp DNA Ladder50323 500 – 8,000 bp DNA Ladder50631 50 – 2,500 bp DNA Marker
7
High efficiency in-gel cloning from SeaPlaque® GTG® Agarose
8.0 x 107 –
7.0 x 107 –
6.0 x 107 –
5.0 x 107 –
4.0 x 107 –
3.0 x 107 –
2.0 x 107 –
1.0 x 107 –
0 –
No. T
rans
form
ants
per
µg
of v
ecto
r
In-Gel Recovered
Two lanes of Bst E II digest of lambda DNA were electrophoresed in a 1% SeaPlaque® GTG® Agarose gel prepared in 1X TAE. The 2.3 kb band was excised from each lane. One sample was ligated directly to pUC 19 in the presence of remelted agarose (In-gel) and the other sample was purified using a chaotrope-based recovery protocol (Recovered). Transformation into DH5αTM Cells was done following standard protocols.
10
Special Applications
Agarose for protein separation Lonza offers a range of agarose types designed for specific, high performance protein applications. Agarose offers distinct advan-tages over polyacrylamide for protein analysis. Agarose effectively separates high molecular weight proteins and protein complexes (>600 kDa), and provides high recovery yields with simple procedures. Agarose is safer and easier to work with.
Agarose Cat. No. Size Typical Application Protein Size Range (kDa) Gel Concentration
IsoGel® Agarose Isoelectric focusing of proteins
■ Highly purified with no measureable EEO■ Versatile for casting in vertical tubes, vertically molded, or horizontally open cast thin gel
Ordering Information
Cat. No. Size
50202 25 g
Routine Protein Separation
Agarose Cat. No. Typical Application Protein Size Range (kDa) Gel Concentration
MetaPhor® Agarose 501815018050184
Protein electrophoresis 20 – 200150 – 300300 – 600
4%3%2%
SeaKem® Gold Agarose 5015250150
600 – 1,0001,000 – 5,000
1.5%1%
SeaPlaque® Agarose 5010150100
600 – 1,0001,000 – 5,000
1.5%1%
Specialty Protein SeparationIsoGel® Agarose 50502 Isoelectric focusing Separation based on
SeaKem® HGT Agarose 5004150040
Counter-Immunoelectrophoresis
SeaKem® HGT(P) Agarose 50050 Factor VIII/von Willenbrand’s
SeaKem® ME Agarose 500115001050014
Serum protein electrophoresis
SeaKem® HEEO Agarose 5003150030
Immunoelectrophoresis of IgG
SeaKem® HE Agarose 5002150020
Serum protein electrophoresis,
Size
25 g125 g500 g
25 g125 g
25 g125 g
25 g
25 g125 g
125 g
25 g125 g500 g
25 g125 g
25 g125 g
SeaKem® ME Agarose Serum protein and IE P analysis
■ Enhanced resolution for serum protein electrophoresis■ High gel clarity and minimal non-specific binding■ Broad range, multipurpose agarose for DNA separation
Ordering Information
Cat. No. Size
50011 25 g
50010 125 g
50014 500 g8
isoelectric point
(CIEP Crossed-IEP)
factor multimer separation
and IgM
IEP, Crossed-IEP, CIEP
Endotoxin TestingSpecial Applications
I.D.na® Agarose High performance identity testing
■ Capable of discerning VNTRs, HVRs, RFLPs and DNA size standards■ Strong, easy to handle gels for trouble-free, high efficiency blotting■ Crisp DNA band separation
Agarose Cat. No. Size Typical Application Protein Size Range (kDa) Gel Concentration
Ordering Information
Cat. No. Size
50170 125 g
SeaPrep® Agarose Agarose for cell culture
■ Ultra-soft agarose for high efficiency hybridoma cloning■ Highly purified and free from inhibitory contaminants■ Soft gel texture, high gel clarity and low gelling temperature ■ Ideal suspension medium for cells
Ordering Information
Cat. No. Size
50302 25 g
Specifications
■ Melting temp: ≤50°C @ 1%■ Gelling temp: 8°C - 17°C @ 0.8%■ Gel Strength: >75 g/cm2 @ 2%
9
www.lonza.com
Lonza DNA Markers and Ladders – Sizing Made Easy
Cat. No.
Standard 50330 50320 50461 50631 50321 50322 NA 50323 50471 50334 50335 50575
SimplyLoad® 50331 50326 NA NA 50327 50328 50333 50329 NA 50336 50337 NALadders
20 bp
500 bp
200 bp
100 bp
20 bp
50 - 1,000 bp
1,000 bp
300 bp
100 bp
50 bp
50 - 2,500 bp
2,500 bp
1,000 bp
400 bp
50 bp
100 bp
1,000 bp
500 bp
100 bp
100 bp Ext
3,000 bp
1,000 bp
500 bp
100 bp
Tandem
10 kb
3,000 bp
1,000 bp
500 bp
500 bp
5,000 bp
2,000 bp
1,000 bp
500 bp
1 - 10 kb
10 kb
5 kb
1 kb
QuantLadder
1,000 bp100 ng
1,000 bp10 ng
500 bp50 ng
500 bp50 ng
100 bp10 ng 100 bp
100 ng
Reverse QuantLadder
RNA Marker
9 kb
6 kb
5 kb
4 kb
3 kb
2.5 kb
2 kb
1.5 kb
1 kb
0.5 kb
20 bp Ext
1,000 bp
500 bp
200 bp
20 bp
100 bp
SimplyLoad® Ladders are supplied in ready-to-load concentrations.
Contact Information
North AmericaCustomer Service: 800 638 8174 (toll free)Scientific Support: 800 521 0390 (toll-free) scientific.support@lonza.comOnline Ordering: www.lonza.com
EuropeCustomer Service: + 32 87 321 611Cell DiscoveryScientific Support: + 49 221 99199 400 scientific.support.eu@lonza.comMolecular Biology, RTS & MediaScientific Support: + 32 87 321 611 scientific.support.eu@lonza.comOnline Ordering: www.lonza.com
InternationalContact your local Lonza DistributorCustomer Service: + 1 301 898 7025, ext. 2322 scientific.support@lonza.com
International OfficesAustralia + 61 3 9550 0883Austria 0800 201 538 (toll free)Belgium + 32 87 321 611Brazil + 55 11 2069 8800Denmark + 45 43 56 74 00France 0800 91 19 81 (toll free)Germany 0800 182 52 87 (toll free)India + 91 22 4342 4000Italy + 39 0363 45710Japan + 81 3 5566 0612Poland + 48 22 833 87 45Singapore + 65 64914214Spain + 34 902 531 366Sweden 020 140 4410 (toll free)Switzerland 0800 83 86 20 (toll free)The Netherlands 0800 022 4525 (toll free)United Kingdom + 44 118 979 5234
Lonza Rockland, Inc. Rockland, ME 04841 DH5α is a registered trademark of Life Technologies, Inc. SeaKem is a registered trademark of FMC Corporation. ¦ Bio-Rad and CHEF-DR are registered trademarks of Bio-Rad Laboratories, Inc. ¦ GeneAmp is a trademark of Roche Molecular Systems Inc.All other trademarks herein are marks of the Lonza Group or its affiliates.
Lonza group or its subsidiaries are owners of the following patents. Products described herein may be covered by one or more of these United States patents, by pending patent application, or in other countries. D510,770: D511,386: D524,449: 6,365,34L
Lonza products are sold either “For Research Use” or “For Laboratory Use”. Please contact a Technical Service Specialist for a complete list of products that are sold “For Laboratory Use”.
The information contained herein is believed to be correct and corresponds to the lates t s tate of scientif ic and technical knowledge. However, no warranty is made, either expressed or implied, regarding its accuracy or the results to be obtained from the use of such information and no warranty is expressed or implied concerning the use of these products. The buyer assumes all risks of use and/or handling. No statement is intended or should be construed as a recommendation to infringe any existing patent.
© Copyright 2009, Lonza Rockland, Inc. All rights reserved. Br-LonzaAgarose 05/09
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