living organisms consist of cells state the resolution and magnification that can be achieved by a...

Living Organisms Consist of Cells

•State the resolution and magnification that can be achieved by a light microscope•Explain the difference between magnification and resolution•Explain the need for staining samples in light microscopy

Resolution and Magnification

Car Headlights from far away Car Headlights Close-Up

Define the Following Terms

• Magnification• Resolution

Light Microscope

• Number of lenses used to produce an image

• Light passes from the bulb (or mirror) under the stage through a condenser lens and then through the specimen

• The light beam is focused through the objective lens then the eyepiece lens

• How do you calculate the magnification power of a light microscope?• Eyepiece lens magnification x Objective lens magnification

• The max magnification of a light microscope is x1500

• The max resolution of a light microscope is 200nm

An Electron Microscope

Chloroplasts Electron micrograph

Leaf cells in moss ; viewed using light Microscope; chloroplasts visible, but not detailed

Optical microscope image of blood cell smear.

SEM image of red blood cells.

TEM image of a thin section of a blood vessel. A white blood cell is entering the tissue between two epithelial cells.