international conference embryonic and somatic stem … · spanholtz,jan,...
Post on 21-Aug-2018
217 Views
Preview:
TRANSCRIPT
NOVA ACTA LEOPOLDINAAbhandlungen der Deutschen Akademie der Naturforscher Leopoldina
Im Auftrage des Presidiums herausgegeben von
Harald zur Hausen
Vizeprasident der Akademie
NEUEFOLGE NUMMER352 BAND 95
International Conference
Embryonic and Somatic Stem Cells -
Regenerative Systems for Cell and Tissue
Repair
organized by the German Research Foundation
and the German Academy of Sciences Leopoldina
International Congress Center Dresden, Germany
September 24-27, 2006
2nd Biennial Leopoldina Conference and Final Meeting of
the Stem Cell Priority Program 1109 of the German Research
Foundation (DFG)
Editors:
Volker ter Meulen (Wtirzburg - Halle/Saale)President ofthe Academy
Anna M. Wobus (Gatersleben) J!^!"^Member ofthe Academy
' w
With 1 Figure
Deutsche Akademie der Naturforscher Leopoldina, Halle (Saale) 2006
In Kommission bei Wissenschaftliche Verlagsgesellschaft mbH Stuttgart
Inhalt
Welcome 17
Organizing Committees 18
Scientific Program 19
Lecture Abstracts
Keynote Lecture:
Jaenisch, Rudolf: Nuclear Cloning, Embryonic Stem Cells and Cell Therapy: Promise,Problems, Reality 29
Introductory Guest Lecture:
McKay, Ronald: Stem Cells and Regenerative Medicine 31
SESSION 1
Stem Cells and Pluripotency
Wu, Guangming, Gentile, Luca, Do, Jeong Tae, Fuchikami, Takuya, Abe, Kuniya, and
SchOler, Hans R.: Consequences of Failure to Restrict Pluripotency in Mouse
Preimplantation Embryos 32
Lemischka, Ihor: Exploring Cell Fate Regulation in Stem Cells 34
Orkin, Stuart H., Wang, Jianlong, Rao, Sridhar, Chu, Jianlin, Shen, Xiaohua, and Theu-
nissen, Thorold W.: An Interactome for Pluripotency of Embryonic Stem Cells....
35
SPP Member Short Reports:
Quiroga-Neoreira, Angel, and Besser, Daniel: Molecular Mechanisms Involved in the
Maintenance of the Undifferentiated State in Human Embryonic Stem Cells 36
Greber, Boris, Babaie, Yasmin, Herwig, Ralf, Brink, Thore C, Wruck, Wasco, Groth,
Detlef, Lehrach, Hans, Burdon, Tom, and Adjaye, James: RNAi-mediated Sup¬
pression ofOCT4 Function in Human ES Cells Recapitulates Primary Differentia¬
tion in the Blastocyst 38
SESSION 2
Stem Cell Biology
Potten, Chris: Stem Cells in the Intestine, Numbers, Location and Characteristics 39
5
Blau Helen: Epigenetic Reprogramming in Non-dividing Somatic Cell Heterokaryons40
Barde, Yves-Alain: Using Embryonic Stem Cells to Study Neural Development 41
SPP Member Short Reports:
Egger-Adam, Diane, Gryzik, Tanja, and Wodarz, Andreas: Functional Analysis of
Binding Partners of Bazooka/PAR-3 during Asymmetric Division of Neural Stem
Cells in Drosophila 43
Huttner, Wieland B.: The Cell Biology of Neurogenesis 44
Corbeil, Denis: The Stem Cell Marker Prominin-1 (CD133) - A New Tool for Tissue
Engineering 45
Spanholtz, Jan, Levetzow, Gregor von, Caddedu, Ron-Patrick, Beckmann, Julia, Scheit-
za, Sebastian, Wernet, Peter, Punzel, Michael, and Giebel, Bernd: NUMB, a Cell
Fate Determinant Known from Drosophila melcmogaster, Inhibits Maintenance of
Primitive Human Hematopoietic Cell Fates 47
SESSION 3
Embryonic Stem Cells and Differentiation
Pedersen, Roger: Mechanisms of Pluripotency and Epigenesis in Human EmbryonicStem Cells 48
Boheler, Kenneth R.: Identification of a Novel Transcription Factor Implicated in the
Maintenance and Differentiation of Pluripotent Embryonic Stem Cells 49
SPP Member Short Reports:
Brostle, Oliver, Ladewig, Julia, Driehaus, Johanna, Opitz, Thoralf, Couillard-
Despres, Sebastien, Biegler, Andrea, Steinfarz, Barbara, Aigner, Ludwig, Itsko-
witz-Eldor, Josef, and Koch, Philipp: Derivation of Stable Neural Stem Cells from
HumanES Cells 50
Wobus, Anna M.: ES Cell Generation of Insulin-producing Cells and Characterization
of Potential Pancreatic Progenitors 51
SESSION 4
Embryonic Stem Cells and Differentiation (continued)
Soria, Bernat: Cell Therapy of Diabetes mellitus: Insulin Secreting Cells from Stem
Cells 53
Field, Loren J.: Cardiac Repair 54
SPP Member Short Reports:
Sciirickel, Jan Wilko, Roll, Wilhelm, Becher, Ulrich, Hashemi, Toktam, Breitbacii,Martin, Sasse, Philipp, Welz, Armin, Lewalter, Thorsten, and Fleischmann,Bernd: Cellular Cardiomyoplasty Alters Ventricular Vulnerability after MyocardialInfarction
55
6
David, Robert, and Franz, Wolfgang-Michael: Enrichment and Isolation of In vitro
Differentiated ES Cell Derived Cardiomyocytes and Cardiac Precursor Cells for
Transplantation into the Infarcted Myocardium 56
Saric, Tomo: Immunologic Properties of Undifferentiated and Differentiated Murine
ES Cells 58
Special Lecture
Dick, John E.: Cancer Stem Cells: Lessons from Leukemia 59
SESSION 5
Adult Stem Cells and Functional Properties
Fuchs, Elaine: Stem Cells and their Lineages in Skin 60
SPP Member Short Reports:
MOller, Albrecht M., and Vallabhapurapu, Duttu: Transient HDAC-Inhibitor Treat¬
ment Increases Hematopoietic Progenitor/Stem Cell Activities 61
Cross, Michael, and Alt, Riidiger: Hematopoietic Stem Cell Metabolism 62
Zenke, Martin, Hieronymus, Thomas, Ensenat-Waser, Roberto, Jaenti, Piritta, Ju,
Xin-Sheng, Ober-Bloebaum, Julia, and Sere, Kristin: Stem Cell Commitment and
Differentiation in Hematopoiesis 63
SESSION 6
Adult Stem Cells and Functional Properties (continued)
Alison, Malcolm R., Direkze, Natalie, Brittan, Mairi, and Wright, Nick: The Role of
the Bone Marrow in Fibrogenesis and Vasculogenesis 65
SPP Member Short Reports:
Braun, Thomas: Mechanisms of Myocardial Regeneration 67
Koyanagi, Masamichi, Bushoven, Philipp, Iwasaki, Masayoshi, Aicher, Alexandra, Zei-
her, Andreas M., Haendeler, Judith, and Dimmeler, Stefanie: Regulation of En¬
dothelial Progenitor Cells Differentiation by Wnt-dependent Signaling 68
Schroeder, Timm, Schwanbeck, Ralf, Meier-Stieoen, Franziska, Henning, Konstanze,
Erfurth, Marie-Luise, Herring, Johanna, Rieber, Nikolaus, Kohlhof, Hella,
Nishikawa, Satomi, Bornkamm, Georg W., Nishikawa, Shin-Ichi, and Just, Ursu¬
la: Molecular Mechanisms of Lineage Decisions Mediated by Notch Signaling in
Adult and Embryonic Hematopoietic Development 69
SESSION 7
Neural Stem Cells
Frisen, Jonas: Neural Stem Cells and Neurogenesis in the Adult Brain 70
Alvarez-Buylla, Arturo: Regulation ofAdult Neural Stem Cells: Role of PDGF Re¬
ceptors 71
7
SPP Member Short Reports:
Buffo, Annalisa, Rite, Inmaculada, Mori, Tetsuji, Dimou, Leda, Lepier, Alexandra, and
Gotz, Magdalena: Glia Cells Generate Neurons: Molecular Mechanisms ofNeu¬
rogenesis 72
Kempermann, Gerd: Gene Networks in the Control of Adult Hippocampal Neuro¬
genesis 74
SESSION 8
Neural Stem Cells and their Niches
SPP Member Short Reports:
Mazurov, Vladimir, Mchedlishvili, Levan, Echeverri, Karen, Schnapp, Esther, and
Tanaka, Elly M.: The Neural Progenitor Cells for Spinal Cord Regeneration 75
Taylor, Verdon, andBASAK, Onur: Jagged1 Provides a Niche Signal for the Maintenance
of Notch1-dependent Adult Neural Stem Cells in the Subventricular Zone 76
Binder, Ellen, Tsarovina, Nina, Stanke, Matthias, and Rohrer, Hermann: PeripheralNeural Stem Cells: Response to Fate Determining Transcription Factors and Ex¬
trinsic Signals 78
Plate, Karl H.: Common Regulatory Mechanisms of Angiogenesis and Neuro¬
genesis 80
Moritz, Soren, Lehmann, Stefanie, Faissner, Andreas, and Holst, Alexander von: Under¬
standing the Neural Stem Cell Niche: An Induction Gene Trap Approach 81
Ber, Suzan, Mansouri, Ahmed, Hao, Wenlin, Englert, Ulrike, and Neumann, Harald:
Characterization ofAdult Bone Marrow Derived Stem Cells and CNS Therapy Ap¬proach 83
Special Lecture
Oakley, Erin, Liang, Ying, and Van Zant, Gary: Hematopoietic Stem Cell Changes in
Response to Aging 85
SESSION 10
Germ Cells
Engel, Wolfgang: Spermatogonial Stem Cells from Adult Mouse Testis Are Pluri-
potent 86
Kanatsu-Shinohara, Mito, and Shinohara, Takashi: Culture and Genetic Modification
of Mouse Male Germline Stem Cells 87
SPP Member Short Reports:
Hiiragi, Takashi: Mechanism ofFirst Polarity Establishment in the Mouse Embryo 88
Jessberger, Rolf: Towards Understanding Meiosis: New Stem Cell-based Ap¬proaches 90
8
SESSION 11
Stem Cells in Tissue Engineering and Biotechnology
Caplan, Arnold I.: New Tools and Logics for Cell-based Therapies Using Adult Mes¬
enchymal Stem Cells 91
Bang, Anne: From Human ES Cells to Islet: Differentiation of liESC Derived Pancre¬
atic Endocrine Cells Mimics In vivo Development 92
Peerani, Raheem, Purpura, Kelly, Kumacheva, Eugenia, and Zandstra, Peter: Engi¬neering the Embryonic Stem Cell Niche 93
Poster Abstracts (alphabetical order)
100 Anton, Roman, and Kuhl, Michael: Wnt/p-catenin Signaling Supports Sternness
in Murine Embryonic Stem Cells 97
101 Basak, Onur, and Taylor, Verdon: HES5 Mediated NOTCH1 Activity Marks
Neural Stem Cells and/or Radial Glia during Embryonic Development 98
102 Becher, Marc U., Sasse, Philipp, Breitbach, Martin, Garbe, Stefan, Furst, Dieter
0., and Fleischmann, Bernd K.: The Effect of Irradiation on the SubsequentGrowth of Satellite Cells In vitro 99
103 Blyszczuk, Przemyslaw, Kania, Gabriela, Valaperti, Alan, and Eriksson, Urs:
Liver Resident CD45+/CDllb+ Stem/Progenitor Cells Reveal Multilineageand Immunosuppressive Properties 100
104 Boltze, Johannes, Gille, Uwe, Wagner, Daniel, Schmidt, Uwe, Waldmin, Danie-
la, Forschler, Annette, Egger, Dietmar, Schwarz, Johannes, Schwarz, Sigrid,and Emmrich, Frank: Experimental Cell Therapy of Stroke in Small and LargeAnimals 101
105 Bonilla, Sonia, Hall, Anita, Attardo, Alessio, Huttner, Wieland B., and Arenas,
Ernest: Lineage Tracing of Dopaminergic Neurons in the Developing Ventral
Midbrain 103
106 Braun, Jan Matthias, Brulport, Marc, Bauer, Alexander, Hengstler, Jan,
Bussmann, Bianka, Ahnert, Peter, Heinrich, Michael, Faber, Sonya, and
Emmrich, Frank: Successful Induction of Immunological Tolerance of an
Allogeneic and Xenogeneic Cell Therapy Model in Fully ImmunocompetentC57BL6Mice 104
107 Breitbach, Martin, Hashemi, Toktam, Roell, Wilhelm, Xia, Ying, Dewald, Oli¬
ver, Nygren, Jens M., Fries, Jochen W. U., Bohlen, Heribert, Jacobsen, Sten
E. W., Hescheler, Juergen, Welz, Armin, Bloch, Wilhelm, and Fleischmann,
Bernd K.: MSCs Lack Tissue-restricted Differentiation after Transplantation
into the Infarcted Heart 105
108 Buhl, Sandra, Peitz, Michael, Edenhofer, Frank, and Schorle, Hubert: The Re¬
quirement of AP-2y for Trophoblast Stem Cell Determination, Maintenance
and Differentiation 106
9
109 Buhnemann, Claudia, Bernreuther, Christian, Diiine, Marcel, Braun, Holger,
Malik., Christoph Y, Scholz, Andreas, Schachner, Melitta, and Reymann,
Klaus G.: Survival and Differentiation into Neurons ofTransplanted ES Cell-
derived Precursors within Stroke Lesion 107
110 Cappello, Silvia, Schmid, Marie-Theres, and Gotz, Magdalena: The Role ofAd¬
herens Junctions and their Signaling Complexes in the Maintenance of Neural
Stem Cells 108
111 De Pietri Tonelli, Davide, Calegari, Federico, Fei, Ji-Feng, Nomura, Tadashi,
Osumi, Noriko, Heisenberg, Carl-Philipp, and Huttner, Wieland B.: Single-
cell Detection of microRNAs in Developing Vertebrate Embryos after Acute
Administration of a Dual Fluorescence Reporter/Sensor Plasmid 109
112 Dietrich, Jens-Erik, and Hiiragi, Takashi: Stochastic Patterning in the Early
Mouse Embryo 110
113 Dorr, Wolfgang, Gabriel, Peggy, Kuschel, Margret, Pickert, Andrea, and Siege-
mund, Amiett: Mobilization of Bone Marrow Stem Cells Modulates Oral Mu¬
cositis (Mouse) during Fractionated Irradiation Ill
114 Ensenat-Waser, Roberto, Ruau, David, Hacker, Christine, Sciimittwolf, Carolin,
Rolletschek, Alexandra, Hieronymus, Thomas, Wobus, Anna M., MOller,
Albrecht M., and Zenke, Martin: Epigenetic Modification of NeurosphereCells Induces Embryonic and Hematopoietic Stem Cell Genes 112
115 Erturk, Dilek, Pfeifer, Alexander, Mori, Tetsuji, and GOtz, Magdalena: Smad4
Is Required for Progression of Adult Neural Stem Cells towards Neuro¬
genesis 113
116 Falk, Anna, Frisen, Jonas, and Zupicich, Joel: Rapid Fligh-throughput Screen for
Genes Directing Embryonic Stem Cell Lineage Choice 114
117 Finkensieper, Andreas, Hannig, Madeleine, Grabner, Rolf, Habeniciit, Andreas,
Sauer, Heinrich, and Wartenberg, Maria: Potential Role ofthe 5-Lipoxygen-ase (5-LO) Pathway in Mouse Embiyonic Stem Cell (ESC)-derived Embryoid
Body (EB) Differentiation and Angiogenesis 115
118 Flick, Burkhard, Scholz, Patrick, and Klug, Stephan: Identification of Supple¬
ments to Replace Serum in the Culture Medium for the Differentiation of Em¬
bryonic Stem Cells (ESC) into Cardiomyocytes 116
119 Ganz, Julia, Kaslin, Jan, Grandel, Heiner, Kroehne, Volker, Wenzel, Isabell, and
Brand, Michael: Adult Neurogenesis in the Zebrafish Brain 117
120 Giselbrecht, Stefan, TruckenmOller, Roman, Weibezahn, Karl-Friedrich, Welle,
Alexander, and Gottwald, Eric: SMART-processed Scaffolds for Stem Cell-
based Tissue Engineering 118
121 Glauche, Ingmar, Lorenz, Ronny, Loeffler, Markus, and Roeder, Ingo: Quanti¬tative Lineage Specification: Mathematical Modeling and Biological Implica¬tions 120
122 Gocza, Elen, Catunda Lemos, Ana Paula, Hiripi, Laszlo, Bodrogi, Lilla, and Bosze,Zsuzsanna: Characterization ofRabbit Embiyonic Stem Cell Like Cells 121
10
123 Gottwald, Eric', Zimmer, Gerald, and Weibezahn, Karl-Friedrich: Perfused Multi-
well-type Bioreactors for the Organotypic Culture ofCells 122
124 Grandel, Heiner, Wenzel, Isabell, Lohs, Claudia, and Brand, Michael: Proliferat¬
ing Neural Stem Cells in the Vagal Lobe of Juvenile Zebrafish 124
125 Guan, Kaomei, Wagner, Stefan, Maier, Lars S., Nayernia, Karim, Engel, Wolf¬
gang, and Hasenfuss, Gerd P.: Spermatogonia! Stem Cells Differentiate into
Cardiovascular Lineages and Generate Functional Cardiomyocytes 125
126 Hannig, Madeleine, Ruhe, Carola, Vigants, Armands, Finkensieper, Andreas, and
Wartenberg, Maria: Leukocyte Differentiation in Murine Embryoid Bodies
as In vitro Model for Cellular Defence 126
127 Hareng, Lars, Stummann, Tina, Pelizzer, Cristian, Hartung, Thomas, and Bremer,Susanne: Integration ofEmbryonic Stem Cell Based Tests into a Testing Strat¬
egy for Developmental Toxicity Testing 127
128 Heine, Peer, Bumsted O'Brien, Keely, Engelkamp, Dieter, and Schulte, Dorothea:
A Role for the TALE-homeodomain Transcription Factor Meis2 in Embryonicand Adult Neurogenesis 128
129 Hirsch, Cordula, Campano, Louise M., Woi-irle, Simon, and Hecht, Andreas:
Canonical Wnt Signaling Enhances Neurogenesis from Neonatal Neural Pro¬
genitors by Stimulating Proliferation of Committed Precursor Cells 129
130 Horn, Matthias, Loeffler, Markus, and Roeder, Ingo: Mathematical Modeling of
Imatinib Treatment in Patients Suffering from Chronic Myeloid Leukemia....
130
131 Jawerka, Melanie, Zimmermann, Stephan, Gottlicher, Martin, Wurst, Wolfgang,and Gotz, Magdalena: The Role of Histone Deacetylase 2 in Neural Stem
Cells 131
132 Kania, Gabriela, Blyszczuk, Przemyslaw, Valaperti, Alan, Heberer, Michael, and
Eriksson, Urs: Signaling Dependent Differentiation of Heart-derived Immu¬
nosuppressive, Multilineage Stem/Progenitor Cells 132
133 Kaslin, Jan, Grandel, Heiner, Ganz, Julia, Kroehne, Volker, Wenzel, Isabell, and
Brand, Michael: Neural Stem Cells and Neurogenesis in the Adult Zebrafish
Brain: Origin, Proliferation Dynamics, Migration and Cell Fate 133
134 Keil, Marlen, Wulf, Annika, Eckert, Klaus, Uckert, Wolfgang, and Fichtner,
Iduna: Comparison of Gene Transfection Methods in Cord Blood Derived
CD34+ Stem Cells for: In vivo Imaging 134
135 Keller, Catharina, Koch, Philipp, Hack, Michael, GOtz, Magdalena, and Brust-
le, Oliver: Pax6-mediated Neuronal Specification in Embryonic Stem Cell
Cultures 135
136 Kesseler, Daniela, and Edenhofer, Frank: Conditional Loss ofBMPPJA Indicates
Essential Roles for BMP in ES Cell Self-renewal and Early Neural Commit¬
ment 136
137 Khattak, Shahryar, Richter, Tobias, Sobkow, Lidia, Herklotz, Stephan, and Ta-
naka, Elly M.: Transgenesis as a Tool to Study Cell Lineages during Axolotl
Regeneration 137
11
138 Kim, Soya, Moussian, Bernard, Luschnig, Stefan, and Wodarz, Andreas: Analysisof New Mutant Alleles of Atypical Protein Kinase C in Drosophila Neural
Stem Cells 138
139 Klempin, Friederike, Babu, Harish, and Kempermann, Gerd: Serotonin ReceptorStimulation Differentially Regulates Adult Hippocampal Neurogenesis 139
140 Knapp, Dunja, Epperlein, Hans H., Habermann, Bianca, and Tanaka, Elly M.:
Molecular Analysis ofAmphibian Regeneration 140
141 Koch, Carmen, Egger-Adam, Diane, and Wodarz, Andreas: Analysis of a New
Interaction Partner ofthe Bazooka Protein in Drosophila Neurogenesis 141
142 Koch, Philipp, Ladewig, Julia, Biegler, Andrea, Opitz, Thoralf, Driehaus, Johan¬
na, Steinbeck, Julius, and Brustle, Oliver: Fate Restriction and Plasticity of
Human ES Cell-derived Neural Stem Cells 142
143 Koch, Sina, Ryser, Martin, Bornhauser, Martin, Ehninger, Gerhard, and Tihede,Christian: Differences in the Localization of Receptor Tyrosine Kinase FLT3 in
Human Hematopoietic Stem and Progenitor Cells and Leukemic Blasts 143
144 Konig, Susanne, Boiirmann, Johannes, and Sippel, Albrecht E.: Regulation of
Myelopoesis in an In vitro System Based on Mouse Embryonic Stem Cells..
144
145 Kramer, Jan, Steinhoff, Jiirgen, Fricke, Lutz, and Rohwedel, Jtirgen: Modulation
of ES Cell-derived Nephrogenesis 145
146 Kroehne, Volker, Kaslin, Jan, Ganz, Julia, Grandel, Heiner, and Brand,Michael: Proliferation and Cell Fates during Regeneration of the Adult Ze¬
brafish Brain 146
147 Kurth, Ina, Franke, Katja, Pompe, Tilo, Bornhauser, Martin, and Werner,
Carsten: Mimicking the Niche - Adhesion of Hematopoietic Stem Cells onto
Extracellular Matrix Coatings 147
148 Kuschel, Margret, Siegemund, Annett, Noack, Ruth, Gabriel, Peggy, and Dorr,Wolfgang: Transplantation of Bone Marrow Ameliorates Oral Mucositis
(Mouse) after Fractionated Radiotherapy 148
149 Ladhoff, Juliane, Bader, Michael, Doebis, Cornelia, Schmitt-Knosalla, Isabela,
Reiser, Jakob, Volk, Flans-Dieter, and Seifert, Martina: Immunogenicity of
Rat Stem Cells and their Endothelial Derivatives 149
150 Lepperdinger, Giinter, Fehrer, Christine, Laschober, Gehrhard T, and Brunauer,Regina: Mesenchymal Stem Cell Aging 151
151 Lie, Dieter Chichung, Colamarino, Sophia A., Song, Hongjun, Mira, Helena,
Consiglio, Antonella, Jessberger, Sebastian, and Gage, Fred H.: Wnt Signal¬ing in Adult Hippocampal Neurogenesis 152
152 Manns, Martina, Bichler, Zoe, and Heumann, Rolf: Ras-mediated Downregula-tion ofAdult Hippocampal Neurogenesis in Male synRas Mice Is Attenuated
in Females 153
153 Mazurov, Vladimir: Reconstirution of Fully Functional Spinal Cord of Axolotl
with Neural Stem Cells 154
12
154 Mohseni, Paria, Smukler, Simon, Schroeder, Insa, Rolletschek, Alexandra, van
der Kooy, Derek, Wobus, Anna M., and Nagy, Andras: Role of Nestin in Ear¬
ly Steps ofDifferentiation 155
155 Narkilahti, Susanna, Huttunen, Tuomas, Rajala, Kristiina, Piiilajamaki, HanL
Suuronen, Riitta, Hovat ta, Outi, and Skottman, Heli: Continuous Monitoring
System for Human Embryonic Stem Cells Derived Neuronal Cells 156
156 Nikam, Vandana, Ahlbrecht, Katrin, Wfjssmann, Norbert, Koenig, Peter, Loh-
meyer, Jurgen, Seeger, Werner, and Voswinckel, Robert: Characterization of
Circulating Fibrocytes from the Human and Murine Circulation 157
157 Nikolova, Teodora, Wu, Minyao, Brumbarov, Krassimir, Alt, Riidiger, Opitz,
Heike, Boheler, Kenneth R., Cross, Michael, and Wobus, Anna M.: WNT-
conditioned Media Differentially Affect the Proliferation and Differentiation
ofCord Blood-derived CD133+ Cells In vitro 158
158 Nolden, Lars, Edenhofer, Frank, Haupi, Simone, Kocn, Philipp, Wunderlich,
Thomas, Siemen, Flenrike, and Brustle, Oliver: Cre Protein Transduction: A
Tool for Site-specific Recombination in Human Embryonic Stem Cells and
their Neural Progeny 159
159 Overall, Rupert, Jessberger, Sebastian, and Kempermann, Gerd: Involvement of
Cdk5 in the Functional Maturation ofAdult Mouse Hippocampal Neural Stem
Cells 160
160 Peitz, Michael, MOnst, Bemhard, Winnemoller, Dirk, Bosnali, Manal, and Eden¬
hofer, Frank: Stem Cells under Remote Control - Modulating Cellular Func¬
tions by Protein Transduction 161
161 Peter, Roland, Behensky, Christian, Halbwirth, Florian, Landertinger, Elisa¬
beth, and Schurmann, Wolfgang: Stem Cells in Flatworms: Isolation, Charac¬
terization and Cultivation of Neoblasts from Planarians 162
162 Pruszak, Jan, Sonntag, Kai-Christian, van Arensbergen, Joris, Yoshizaki, Taka-
hito, Aung, Moe Hein, Sanchez-Pernaute, Rosario, and Isacson, Ole: Flow
Cytometric Analysis and Purification ofNeural and Neuronal Cell PopulationsDerived from Human Embryonic Stem Cells 163
163 Rieger, Michael A., Hermann, Andrea C, and Schroeder, Timm: Long-term.
Time-lapse Imaging: Tracking ofAdult Hematopoietic Stem Cells at the Sin¬
gle Cell Level 164
164 Rodriguez, Gerardo Ramirez, and Kempermann, Gerd: The Small Heat Shock
Protein b8 (Hspb8) Participates in the Neural Progenitor Cell Division 165
165 Rolletschek, Alexandra, Wiese, Cornelia, Kania, Gabriela, Navarrete-Santos,
Anne, Anisimov, Sergey V, Steinfarz, Barbara, Tarasov, Kirill V, Brugh,
Sheryl A., Zahanich, Ihor, Ruschenschmidt, Christiane, Blyszczuk, Prze-
myslaw, Heubacu, Jurgen F., Ravens, Ursula, St-Onge, Luc, Braun, Thomas,
BrOstle, Oliver, Boheler, Kenneth R., and Wobus, Anna M.: Signals from
Embryonic Fibroblasts Induce Adult Intestinal Epithelial Cells to form Nestin-
positive Cells with Proliferation and Multilineage Differentiation Capacity In
vitro 166
13
166 Rosowski, Mark, Tschirschmann, Miriam, Falb, Melanie, and Lauster, Roland:
Mesenchymal Condensation in Alginate Hollow Spheres 168
167 Salchert, Katrin, Grimmer, Mila, Kurth, Ina, Drichel, Juliane, Pompe, Tilo,
Bornhauser, Martin, and Werner, Carsten: Fibrillar Collagen Matrices to
Maintain Haematopoietic Progenitor Cells in Culture 170
168 Sauerzweig, Steven,Gunther-Kern, Anja,Reymann,KlausG., andBRAUN,Holger:
Generation ofNeurospheres from Adult Rat Bone Marrow Stem Cells 171
169 Schmelter, Maike, Wartenberg, Maria, and Sauer, Heinrich: Stimulation of Car¬
diovascular Differentiation of Embryonic Stem Cells by Mechanical Strain....
173
170 Schmidt, Anke, Sciieibe, Johanna, Stoll, Anja, and POtzer, Brigitte M.: Adult
Neural Stem Cell Specific Peptides Allow Targeted Adenovirus Vector-medi¬
ated Gene Transfer: New Prospects for Direct In Brain-Cell Therapy 174
171 Schneider, Marlon R., Adler, Heiko, Braun, Joachim, Kienzle, Beate, Wolf,
Eckhard, and Kolb, Hans-Jochem: Canine Blastocyst-derived Stem Cells Can
be Induced to Differentiate towards the Hematopoietic Lineage 175
172 Sciireglmann, Nina, Kleber, Susanne, Letellier, Elisabeth, Klussmann, Stefan,
Wiestler, Benedikt, Krammer, Peter H., and Martin-Villalba, Ana: Resist¬
ance ofNeural Stem/Progenitor Cells to CD95L-induced Apoptosis 177
173 Schroeder, Insa S., Rolletschek, Alexandra, Schulz, Herbert, Huebner, Norbert,
and Wobus, Anna M.: Analysis of Pancreatic Differentiation of EmbryonicStem Cells by Transcriptional Profiling, Real Time RT-PCR, and Immunocy-
tochemistry 178
174 Schroter, Aileen, and Thallmair, Michaela: Influence of Inflammation on the
Behavior ofAdult Spinal Cord Progenitor Cells after Spinal Cord Injury 179
175 Schwarz, Florian, David, Robert, and Franz, Wolfgang-Michael: Promoter As¬
sisted Labeling and MACS-purification of Cardiac Progenitor Cells from Em¬
bryonic Stem Cells 180
176 Sel, Saadettin, Schilling, Martin, Friebel, Katrin, Eiiricii, Dirk, Simm, Andreas,
Nakfiai, Hassan, Kalinski, Thomas, Hoppe, Carsten, Duncker, Gemot I. W.,
and Paulsen, Friedrich: Application of CD34+ Stem Cells and Bone Marrow
Cells Promotes the Healing of Corneal Ulcers 182
177 Stary, Martina, and Weitzer, Georg: Paracrine Promotion of Early Cardiomyo-
genesis by Parietal Endoderm Secreted SPARC and S100A4 183
178 Straube, Werner L., Weissert, Philipp M., Drechsel, David N., and Tanaka, EllyM.: Purification of a Serum Factor that Triggers Cell Cycle Re-entry of Dif¬
ferentiated Multinucleated Newt Myotubes 184
179 Thum, Thomas, Fraccarollo, Daniela, Schultfieiss, Maximilian, Froese, Sabrina,Galuppo, Paolo, Frantz, Stefan, Ertl, Georg, and Bauersachs, Johann: En¬
dothelial Nitric Oxide Synthase Uncoupling Results in Impaired Endothelial
Progenitor Cell Mobilization and Function in Diabetes 185
14
180 Tiedemann, Juliane, Kasper, Grit, Tuischer, Jens, Matziolis, Georg, Perka,
Carsten and Duda, Georg N.: Mechanically Regulated MMPs Might Influence
Mesenchymal Stem Cell Behavior 186
181 To, Sarah, Keough, Rebecca A., and Rathjen, Peter D.: The Role of CRTR-1 in
Pluripotent Stem Cells 187
182 Tonack, Sarah, Rolli-tsctick, Alexandra, Wobus, Anna M., Fischer, Bernd, and
Navarrete-Santos, Anne: Expression of Facilitative Glucose Transporter Iso-
fonns in Mouse D3 ES and P19 EC Cells 188
183 Toth, Sara, Hegyesi, Hargita, and Falus, Andras: Up-regulation ofNanog in His-
tamine-deficient Murine Dennatofibrosarcoma Cells 189
184 Urbaniak, Thomas, Stachelscheid, Harald, Gerlach, Jorg, and Zeilinger, Katrin:
Proliferation of Hepatic Progenitors Isolated from Adult Human Liver in Co-
culture with Mouse Embiyonic Fibroblasts 190
185 Vafaizadeh, Vida, Bednorz, Nadja Lydia, Desrivieres, Sylvane, and Groner,
Bemd: The Contribution of Stat5 to the Regenerative Capacity of Mammary
Epithelial Stem Cells 192
186 Vassilieva, Svetlana, Bindloss, Colleen, Knowles, Katrina, Rathjen, Peter, and
Rathjen, Joy: Formation of Human Primitive Ectoderm-like (hEPL) Cells
from hES Cells 193
187 von Elsner, Sophie, Schmidt, Henning, and Schnabel, Ralf: Maintenance of Germ
Line in C. elegans Embryos 194
188 Wagner, Wolfgang, Wein, Frederik, Saffrich, Rainer, Faber, Anne, Krause, Ulf,
Seckinger, Anja, Benes, Vladimir, Eckstein, Volker, Maul, Holger, and Ho,
Anthony D.: Human Mesenchymal Stem Cells as a Surrogate Model for the
Stem Cell Niche 195
189 Weitzer, Georg, and Stary, Martina: Morphogenesis in Embryoid Bodies? 197
190 Wulf, Annika, Eckert, Klaus, and Fichtner, Iduna: Differential Organ Engraft-ment of CD34+ Cord Blood Stem Cells in NOD/SCID Mice 198
191 WOnsch, Annegret, Habermann, Felix A., Klose, Regina, Zakhartchenko, Valeri,
Reichenbach, Horst-Dieter, Sinowatz, Fred, and Wolf, Eckhard: Analysis of
Epigenetic Reprogramming after Somatic Cell Nuclear Transfer in Cattle Us¬
ing an Oct4-EGFP Reporter Gene Construct 199
192 Zeilinger, Katrin, Stachelscheid, Harald, Lubberstedt, Marc, Rossberg, Ingrid,
Urbaniak, Thomas, Knospel, Fanny, Neuhaus, Peter, and Gerlach, Jorg C:
3D Bioreactor Culture Technology for Expansion and Tissue-specific Differ¬
entiation of Stem Cells 201
193 Zietlow, Caroline, Ateghang, Bernadette, Sauer, Heinrich, and Wartenberg,
Maria: Cardiomyocyte Differentiation after Ascorbic Acid Treatment - Inves¬
tigation of Mechanisms ofAction 203
List of Participants
1
top related