innate immune signaling - biolegend...aim2 asc foreign dsdna jak1 jak1 tyk2 tyk2 stat1 stat2 trf9...

* Mouse only

TRAF6TRAF6

TRAF6

EndocytosedTLR4

TLR7/8

TLR13

TLR9

ssRNA

Unmethylated CpG DNA

Unmethylated CpG DNA

dsRNA

TLR3

TRIFTRIF

TRAM RIP1

RIP1

23srRNA

TIRAP

Pro�lin

TLR12Homodimer

TLR11/12Heterodimer TRAF3

TRAF3

TRAF3

IKKεTBK-1

ProteasomalDegradation

Ubiquitin

Ubiquitin

RICK/RIP2

RICK/RIP2

Procaspase-1

Procaspase-1

Caspase-1

Pro IL-1βPro IL-18

IL-1β IL-18 In�ammationPyroptosis

NLRP3

TXNIP

TXNIP

Active State

TRX

TRX TXNIPTRX

ROS

Cathepsins

Crystalline Structures

Pannexin-1

P2X7

PAMPs NOX Enzymes

NOD1

NOD2

Ubiquitin

IKKβ

IKKβ

NF-κB

NF-κB

NF-κB

NF-κBActivation

MAPKActivation

NF-κB

NF-κB

NF-κB

IKKα

IKKβ

IKKγ/NEMO

IKKα

IKKα

AP-1 Activation

TBK1

TBK1

pol IIIViral DNA

Viral RNA

STING

IntracellularBacteria

Proin�ammatoryFactors

Cyclic Dinucleotides

NEMO

IRAK1 IRAK2

IRAK4

LPS

TIRAPMyD88

MyD88

MyD88

?

Lipopeptides

Flagella

TLR5

TLR10TLR2

TLR2

TLR1/6

CD14

CD14

TLR4/MD2

TRAF6

IRAK1 IRAK2

IRAK4IRAK1 IRAK2

IRAK4

TAB2/3

TAB2/3MuramylDipeptide

Endocytosed BacteriaDAMPsPore-forming toxinsCalcium In�ux?

γ-D-glutamyl-mesodiaminopimelic acid

TAK1

TAK1

TAB1

TAB1

TAB2/3

IRF7

ASC

AIM2

ASC

Foreign dsDNA

JAK1

JAK1

Tyk2

Tyk2

STAT1

STAT2

TRF9

ISGF3

IFNStimulatedGenes

Type 1 IFNs

IFNAR1IFNAR2

MAVS Aggregation

Dimerization

TRIM25

Riplet RNF135

K63 Polyubiquitination

Heterotetramer Formation

RIG-I

IRF7

Type 1IFNs

IRF3

IRF3

IRF3

IL-15RANTESCXCL10

IL-1βIL-6IL-10IL-16iNOSTNF-αGM-CSFMIP-1αMIP-1βMCP-1RANTES

TAK1

TAB1

c-JunJNK

p50p60

RelA

c-Fas AP-1

AP-1

AP-1

CREB

RAS

Dectin-1

DC-SIGN

Dectin-2

Mincle

β-glycan

SYK

FcγR

FcγR

α-Mannose,Cord Factor

High Mannose,α-Mannans

High Mannose,Fucose

SYK

SYK

RAF-1

NLRP3 Activation

CARD9

ROS

MALT1Bcl-10

ptd Ins (3, 4, 5) P3

ptd Ins (4, 5) P2

PKC

BTK

RAC

DAGRHO

p38

PKC

InsP3

PLCγ

PLCγ

PI3K

SYKLyn

Antigen

ITAM

IgG Antibodies

FcγRs

α subunit

γ2subunit

SOS

RAS

CGAS

Mda5

IPS-1

ERKJNK

ADCCPhagocytosisCytokine ReleaseOxidative Burst

Ca2+

Ca2+Export

CalcineurinNFAT

NFAT

CREB

p38/MAPK

ParasitesParasites

BacteriaBacteria

BacteriaBacteria

FungiFungi

BacteriaBacteria

VirusesViruses

VirusesViruses

NucleusNucleus

In�ammasomeFormationIn�ammasomeFormation

LysosomeLysosome

MitochondriaMitochondria

EndoplasmicReticulumEndoplasmicReticulumEndoplasmicReticulumReticulumEndoplasmicReticulumEndoplasmicReticulumEndoplasmicReticulumEndoplasmicReticulumEndoplasmicReticulumEndoplasmicReticulumEndoplasmicReticulum

©BioLegend, Inc. 2016

Innate Immune Signaling

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EndosomesEndosomes

Toll - Like Receptor LigandsToll - Like Receptor Ligands

TLR1 - Triacetylated lipopeptidesTLR2 - Glycolipids, lipopeptides, lipoproteinsTLR3 - Double-stranded RNATLR4 - Lipopolysaccharide, heat shock proteins, �brinogenTLR5 - FlagellinTLR6 - Diacyl lipopeptidesTLR7 - Single-stranded RNATLR8 - Single-stranded RNA (Human) Imidazoquinoline, poly T oligonucleotides (Mouse)

TLR9 - Unmethylated CpG DNA, haemozoinTLR10 - ? TLR11* - Flagellin, pro�linTLR12* - Pro�linTLR13* - 23s rRNA

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Interactive Poster: biolegend.com/innate_signaling_posterWe would like to thank Dr. Glen N. Barber of the University of Miami Miller School of Medicine

for his contributions to this poster.