influence of protease enzyme supplementation on apparent ileal digestibility of amino acids of corn,...

Influence of protease enzyme supplementation on apparent ileal digestibility of amino acids of corn,

wheat and barley grainsL. Cámara1,2, M. V. Kimiaeitalab1, J. D. Berrocoso1, C. E. Grewehr1,3, P. Guzmán1, C. R. Angel4, A. Smith5, and G. G. Mateos1,2

1 Departamento de Producción Agraria, U. P. Madrid, 28040 Madrid, Spain2 CEI Campus Moncloa, UCM-UPM, 28040 Madrid, Spain

3 Current address: Universidade do Estado de Santa Catarina, Lages, Brazil4 Department of Animal and Avian Sciences, University of Maryland, College Park, 20742, Maryland

5 DSM Nutritional Products (UK) Ltd., Heanor Gate Industrial Estate, DE75 7SG Heanor, Derbyshire, UK

2,304 one-d-old chicks

Ross 308

4 blocks

12 treatments24 rep (8 chicks)/trt

Main cereal(corn vs. wheat vs.

barley)

Protease(0 vs. 200 mg/kg)

GLU+XYL(0 vs. 100 mg/kg)

Diet

1 to 18 d of ageCommercial diet

18 to 22 d of ageExperimental diet

92% cereal 8% NFD

AID of the AA

Day 22

MATERIALS AND METHODS

  DM Thr Glu Pro Gly Ala Cys Val Met Leu Lys CP

Cereal                        

  Corn 81.3a 66.6 86.6ab 84.5b 72.8 85.9a 76.4 76.2 85.6a 87.3a 76.5 76.4

  Wheat 72.1ab 69.4 92.5a 90.9a 75.2 74.6b 81.8 76.1 82.2ab 82.3ab 73.1 79.7

  Barley 71.3b 66.7 85.4b 84.3b 70.3 72.0b 76.6 74.5 78.4b 78.2b 73.7 74.1

SEM1  2.252   2.772   1.424  1.534   2.568   1.499   2.325   2.270   1.262   1.395   2.138   2.260

 *   NS   * *   NS   **   NS NS   * *   NS  NS

1 n=32 (a cage with 8 chicks)

RESULTSMain cereal of the diet and the AID of the AA, %

DM Thr Glu Pro Gly Ala Cys Val Met Leu Lys CP60.0

65.0

70.0

75.0

80.0

85.0

90.0

+

+

++

*

NS ++

+ NS

NSNS

0 200

DM Thr Glu Pro Gly Ala Cys Val Met Leu Lys CP60

65

70

75

80

85

90

b

b

bb

b

c c

b

b c

bc

a

a

aa

a

ab ab

a

a ab

aaba

a

a a

a

aa

a

a a

a

a

ab

a

a a

a

bb

a

ab b

abb

-(GLU+XYL) -PRO -(GLU+XYL) +PRO

+(GLU+XYL) -PRO +(GLU+XYL) +PROP < 0.001

We  investigated  the  effects  of  protease (Ronozyme  ProAct,  PRO)  supplementation on  the AID    of  3  cereals  in  the  presence  or not  of  a  multi-enzyme  complex  with  β-glucanase and β-xylanase activity (GLU+XYL). The  AID  of most  of  AA was  higher  for  corn and wheat  than  for  barley.  The  inclusion  of GLU+XYL increased the AID of DM, CP and of all the AA, except Met and Lys. The inclusion of PRO increased the AID of Gly and tended to increase that of most of the other AA. The combination  of  both  enzymes  did  not improve further AA digestibility any.

CONCLUSIONS

ABSTRACT

The supplementation of the 2 enzymes in isolation showed some benefits  on AID of  the AA but  no  further  effect was  seen when these were combined. The  inclusion of XYL  improved the AID of DM and of all AA and CP except Met and Lys .The combination of both  enzymes  however,  did  not  improve  AA  digestibility  any further.  Trends  observed  when  combining  different  enzyme activities  in short  term digestibility  trials need  further study and may  not  reflect  the  response  seen  combining  different  enzyme combinations  on  animal  performance  studies  or  digestibility studies  when  fed  as  part  of  a  complete  diet  from  hatch  to slaughter.

Interaction GLU+XYL x protease (mg/kg) on the AID of AA, %

Effect of protease supplementation (mg/kg) on the AID of AA, %

SEM (n=72): 0.404-1.021

SEM (n=144): 0.285-0.722

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