increasing indentation speed
Post on 01-Jan-2016
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increasing indentation speed
AFM indentation
z
2
810
4
6
0.01 0.1 1
2
810
4
6
app
aren
t el
astic
mod
ulus
, E
(kP
a)
contact time (s)
unswollen
shrunken
decreasing indentation speed
A
B
Supplemental figure 2Effective elasticity measured by AFM indentation of immobilized nuclei. Nuclei in the unswollen, swollen, and shrunken state were allowed to adhere to and spread on poly-l-lysine coated glass (A). A sphere-tipped AFM cantilever was then used to indent a given nucleus, yielding force-indentation curves. A height-corrected Hertz sphere model was fit to each curve, giving an apparent elastic modulus for the nucleus (B). Tip indentation speed was increased over 2 orders of magnitude to vary the AFM probe’s contact time with the nuclei (black squares). Tip indentation speed was then reduced to determine the reversibility (i.e. – no plastic deformation) of AFM indentation (gray squares).
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