impact of altered cholinergic tones on the neurovascular coupling

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Research Articles: Systems/Circuits

Impact of altered cholinergic tones on the neurovascular coupling response towhisker stimulation

Clotilde Lecrux1,2, Claire H. Sandoe1, Sujaya Neupane2, Pascal Kropf2, Xavier Toussay1, Xin-Kang Tong1, María

Lacalle-Aurioles1, Amir Shmuel2 and Edith Hamel1

1Laboratory of Cerebrovascular Research2Laboratory of Brain Imaging Signals, Montreal Neurological Institute, McGill University, Montréal, QC, Canada

DOI: 10.1523/JNEUROSCI.1784-16.2016

Received: 25 May 2016

Revised: 22 December 2016

Accepted: 30 December 2016

Published: 9 January 2017

Author contributions: C.L., C.S., A.S., and E.H. designed research; C.L., C.S., P.K., X. Toussay, and X. Tongperformed research; C.L., C.S., S.N., P.K., X. Toussay, X. Tong, and M.L.-A. analyzed data; C.L., C.S., S.N.,M.L.-A., A.S., and E.H. wrote the paper; S.N. and M.L.-A. contributed unpublished reagents/analytic tools.

Conflict of Interest: None declared.

Funding: Supported by grants from the Canadian Institute of Health Research (CIHR, MOP-84209 andMOP-142417, EH; MOP-102599, AS), the Heart & Stroke Foundation of Canada (EH), the Human FrontierScience Program (RGY0080/2008, AS), a Jeanne Timmins Costello Postdoctoral Fellowship (CL), and a JeanneTimmins Costello studentship (CHS).

Corresponding author: Dr. Clotilde Lecrux, Laboratory of Cerebrovascular Research, Montreal NeurologicalInstitute, 3801 University Street, Montréal, QC, Canada H3A 2B4, E-mail: clotilde.lecrux@mcgill.ca, Tel: (+1)514 398 8931

Cite as: J. Neurosci 2017; 10.1523/JNEUROSCI.1784-16.2016

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in vivo

Animal preparation

Cortical blood flow measurement

Electrophysiology

Whisker stimulation

Identification of the neuronal network recruited by whisker stimulation

Enhanced ACh tone

Decreased ACh tone

Hemodynamic signals:

Electrophysiological data alignment:

Electrophysiological signals:

LFP/CBF correlations:

Cholinergic denervation:

C-Fos immunostaining in the barrel cortex:

Identification of c-Fos-immunopositive neurons in the activated barrel cortex:

Statistical Analysis:

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r z

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* *** r z

NK post-test

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