how to make dirty rivers, lakes and seas clean and fresh

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Our world's rivers, lakes, canals and reservoirs can be so polluted that life is destroyed in many countries. A new, simply brillient method to address this problem is now available using safe technology and best practice. This Invention is cool!

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Nualgi, a revolutionary solution for cleaning polluted water bodies

4/25/2013 1 info@nanolandglobal.com

Dr. Didier Kane didier.kane@nanolandglobal.com Mr Dermott Reilly dermott.reilly@nanolandglobal.com +44 20 3287 9234

Agricultural Agricultural & &

Farm RunoffFarm Runoff

Urban Waste Urban Waste WaterWater

SedimentSediment RefluxReflux

NLG D K

Rain Spill

NLG

Eutrophication

Our lakes and rivers are organically polluted Our lakes and rivers are organically polluted by many sources of human activityby many sources of human activity

4/25/2013 2 info@nanolandglobal.com

Disturbing facts about water pollution

• Water pollution is one of the biggest global killers, affecting over 100 million people. That’s comparable to global diseases like malaria and HIV. • Approximately 46% of the lakes in America are too polluted for fishing, aquatic life, or swimming. • The Mississippi River carries an estimated 1.5 million metric tons of nitrogen and 0.5 million metric tons of phosphorus pollution into the Gulf of Mexico each year, creating a “dead zone” in the Gulf each summer about the size of New Jersey. • Each year 1.2 trillion gallons of sewage, storm water, and industrial waste are dumped into U.S. water. • Every day, 2 million tons of human waste are disposed of in water courses.

Source: EPA, WWAP, BlackSmith Institute 4/25/2013 3 info@nanolandglobal.com

What do pollutants do to our lakes?

Cyanobacteria algae decay

2

1

3 Bacteria

Deplete Oxygen

4 Fish die by

lack of oxygen

5

Dead fish creates more cytobacterial

algal bloom

6

nualgi@nanolandglobal.com nualgi@nanolandglobal.com

4/25/2013 4 info@nanolandglobal.com

The vicious circle of eutrophication Excess nutrients (N, P, etc.) from

wastewater, fertilizers, etc,

Excess nutrients (N, P, etc.) from

wastewater, fertilizers, etc,

Exponential growth of

cyanobacteria

Exponential growth of

cyanobacteria

Short-lived cyanobacteria

decays

Short-lived cyanobacteria

decays

Organic decay consumes

oxygen

Organic decay consumes

oxygen

Oxygen-deprived fish die and

become organic sediments

Oxygen-deprived fish die and

become organic sediments

Decay of organic sediments feeds bacteria growth

Decay of organic sediments feeds bacteria growth

N & P sediment and remain

present in lake

N & P sediment and remain

present in lake

4/25/2013 5 info@nanolandglobal.com

Preventing eutrophication

Excess nutrients (N, P, etc.) from

wastewater, fertilizers, etc,

Excess nutrients (N, P, etc.) from

wastewater, fertilizers, etc,

Exponential growth of

cyanobacteria

Short-lived cyanobacteria

decays

Organic decay consumes

oxygen

Oxygen-deprived fish die and

become organic sediments

Decay of organic sediments feeds bacteria growth

N & P sediment

and remain present in lake

XX BMP - Best Management

Practices

Nutrient removal from sewage

4/25/2013 6 info@nanolandglobal.com

Reversing eutrophication Excess nutrients (N, P, etc.) from

wastewater, fertilizers, etc,

Excess nutrients (N, P, etc.) from

wastewater, fertilizers, etc,

Exponential growth of

cyanobacteria

Short-lived cyanobacteria

decays

Organic decay consumes

oxygen

Oxygen-deprived fish die and

become organic sediments

Decay of organic sediments feeds bacteria growth

N& P sediment

and remain present in lake

Phosphorus Inactivation using Alum

Dilution and Flushing

P and N absorption by diatom algae

XX

4/25/2013 7 info@nanolandglobal.com

Reversing eutrophication

Excess nutrients (N, P, etc.) from

wastewater, fertilizers, etc,

Exponential growth of cyanobacteria

Exponential growth of cyanobacteria

Short-lived cyanobacteria

decays

Organic decay consumes

oxygen

Oxygen-deprived fish die and

become organic sediments

Decay of organic sediments feeds bacteria growth

N & P sediment

and remain present in lake

Diatoms are consumed by fish and do not require harvesting

Algaecides

XX

4/25/2013 8 info@nanolandglobal.com

Algae Harvesting

Reversing eutrophication Excess nutrients (N, P, etc.) from

wastewater, fertilizers, etc,

Exponential growth of

cyanobacteria

Short-lived cyanobacteria

decays

Organic decay consumes oxygen

Organic decay consumes oxygen

Oxygen-deprived fish die and

become organic sediments

Decay of organic sediments feeds bacteria growth

N& P sediment

and remain present in lake

Oxygen release by diatom algae

(photosynthesis)

Hypolimneticoxygenation

Artificial circulation (aeration)

XX

4/25/2013 9 info@nanolandglobal.com

Reversing eutrophication

Excess nutrients (N, P, etc.) from

wastewater, fertilizers, etc,

Exponential growth of

cyanobacteria

Short-lived cyanobacteria

decays

Organic decay consumes

oxygen

Oxygen-deprived fish die and become organic sediments

Oxygen-deprived fish die and become organic sediments

Decay of organic sediments feeds bacteria growth

N & P sediment

and remain present in lake

Populate lake with Hypoxia-tolerant fish

XX

4/25/2013 10 info@nanolandglobal.com

Diatoms help native fish survive

Reversing eutrophication

Excess nutrients (N, P, etc.) from

wastewater, fertilizers, etc,

Exponential growth of

cyanobacteria

Short-lived cyanobacteria

decays

Organic decay consumes

oxygen

Oxygen-deprived fish die and

become organic sediments

Decay of organic sediments feeds bacteria growth

Decay of organic sediments feeds bacteria growth

N & P sediment and remain

present in lake

Dredging

Consumption of organic sediments by

diatom algae

XX

4/25/2013 11 info@nanolandglobal.com

Restoration method

Ease of use

effectiveness Environmental

impact Initial cost

Maintenance cost

Overall score

Bio-acceleration (diatom algae)

Phosphorus Inactivation

(Alum)

Flushing (Nutrients dilution)

Algae harvesting

Algaecides

Hypolimnetic oxygenation

Aeration

Bio-manipulation

Dredging

Restoration methods comparison

4/25/2013 12 info@nanolandglobal.com

Most effective Restoration method: Bio-acceleration

What are diatoms ?

• Diatoms are photosynthesizing algae • They come in many different shapes • They are commonly between 20-200 microns in diameter or length • The cell wall is composed, for the most part, of silica (SiO2)

N + P+

4/25/2013 13 info@nanolandglobal.com

Bio-restoration: the most effective way to clean a lake

Cyanobacteria Algae dies by lack of nutrients

2

1

4 Diatoms

provide oxygen to aerobic bacteria

5

7

nualgi@nanolandglobal.com

Aerobic bacteria

breakdown nutrients and

organic matters

aerobic bacteria transform nutrients into zooplankton

Fish feed on zooplankton

8 3 Brokendown nutrients are consumed by diatoms

6 Zooplankton

consume diatoms

4/25/2013 14 info@nanolandglobal.com

Most effective way to grow diatoms

+ Diatom

Silica

Iron

Manganese

Zinc

Other minerals

+

Necessary nutrients (*)

Nitrogen

Phosphorus

Potassium

Excess nutrients

+

Nature

Sunlight

Water

CO2

= = X + (*) NUALGI provides all these nutrients

4/25/2013 15 info@nanolandglobal.com

How to provide the right amount of nutrients ?

• Nutrient uptake kinetics for photosynthetic algae, including diatoms is a function of numerous variables (e.g., amount of sunlight, concentration in P and N, temperature, etc.) • The cell wall of diatoms is constructed by silica deposition vesicles (SDV) with membranes called silicalemmas. • Without the proper amount, size and geometry of silicate, diatom cells cannot divide, and its capacity to perform energy producing processes (photosynthesis and glycolysis) are diminished • The already very small (micron-scale) diatoms have tiny portules and chloroplasts in their cell walls where micronutrients are absorbed and photosynthesis takes place. • Nutrients have to be available in nanoscale geometries to be easily absorbed through these portules.

4/25/2013 16 info@nanolandglobal.com

A revolutionary nanotechnology-based solution !!

• Scientists at Nualgi Nanobiotech have developed a

revolutionary solution for diatom regeneration : NUALGI • This patented technology, based on the latest nanotechnology manufacturing methods and years of algal micro-nutrition research, provides an optimum blend of micronutrients on a nano-silica base.

• The incredibly effective NUALGI provides 10 micro-nutrients with an optimum concentration ratio and nano-size distribution to accelerate diatom regeneration

4/25/2013 17 info@nanolandglobal.com

An extraordinarily effective solution : NUALGI

Initial state before NUALGI One week after NUALGI application

Invasive cyanobacteria (Blue-Green

Algae or BGA) throughout

water column

Increasing diatom population on lake bottom, providing Oxygen to aerobic bacteria

Cleaned water

throughout water

column

Decreasing BGA

population localized near the surface

4/25/2013 18 info@nanolandglobal.com

Before Nualgi treatment After Nualgi treatment

In 6-8 hours, DO increased to 2- 2.5 mg /l and in 4 days DO shot up to 5-10 mg/l.

Example of Nualgi use: Vengayankere lake in Bangalore, India

4/25/2013 19 info@nanolandglobal.com

Some projects where NUALGI has restored lakes

• Madivala Lake, Koramangala, Bangalore, India • Croton-on-Hudson duck-pond, New York, USA • Ulsoor lake and Puttenahalli lake, India

Some projects where NUALGI is being evaluated

• Lagoon clams farming project in Micronesia • Newport harbor, California, USA

4/25/2013 20 info@nanolandglobal.com

Agricultural Agricultural & &

Farm RunoffFarm Runoff

Urban Waste Urban Waste WaterWater

SedimentSediment RefluxReflux

DK DK

D K

Rain Spill

DK

With NUALGI

Our lakes and rivers are polluted by many Our lakes and rivers are polluted by many sources of human activitysources of human activity

DK

4/25/2013 21 info@nanolandglobal.com

Other advantages of using NUALGI

• The most eco-friendly restoration method

• Low cost (after initial treatment of 1 liter per acre per week for 2 weeks, a half liter per acre per week for 4 to 6 weeks is usually enough

• NUALGI can be used in a broad variety of applications: • Fish farming • Sewage treatment • Lakes and streams restoration • Aquariums • Biodiesel • Agriculture and horticulture (in spray form)

4/25/2013 22 info@nanolandglobal.com

Thanks to nanotechnology combined with algal nutrition science, the future of water

bodies restoration is now.

Please contact us for more information or to setup a trial of NUALGI

info@nanolandglobal.com +44 20 3287 9234

7 Bath Place LONDON EC2A3DR

UK

4/25/2013 23 info@nanolandglobal.com

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