genetics and crop improvement varietals selection of cip germplasm in bangladesh august, 2013
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Introduction
This experiment is based in 2 statistical designs: RCBD Observation plot
Block 1Block 2 Block 3
Experimental brief: 15 CIP clone & 2 CV Year: 2011-12
Introduction
.
.
Locality Dinajpur
Locality Debigonj
Plot I
Plot II
Plot III
Rep. I
Rep. II
Rep. III
Plot I
Plot II
Plot III
Rep. I
Rep. II
Rep. III
Objetives
The following objectives are sought with this design:
To gather the opinion of the parties involved in the selection of possible new varieties.
To characterize the preferences that exist in the different regions.
To validate a methodology in order to be able to systematically compare results between sites and different years.
Advantages
Some advantages are:
Ensure that the varieties meet the needs of the breeders and stakeholders.
Early acceptance of varieties.
Early access to greater genetic diversity in breeding materials.
Increased ability to manipulate resources in different environments and seasons.
.1. Vegetative development:
• Identification selection criteria• Ranking selection criteria
2. Harvest:
• Evaluation tuber yield• Identification selection criteria• Ranking selection criteria • Organoleptic evaluation
3. Post-harvest:
• Sprouting & weight loss• Identification selection criteria• Ranking selection criteria
Main exercises / measurements during the selection cycle
VegetativeVegetative
Stage of EvaluationStage of
Evaluation
HarvestHarvest Post-HarvestPost-Harvest
Main exercises / measurements during the selection cycle
Phases
This table shows the phases, components, and methods
Phase Component Method
Trial, materials and site information
Minimal - basic data List
Trial information, management and evaluation data
List
Management calendar ListSoil analysis (#) Soil analysis Climate data (#) Weather stationList of materials List
Flowering (vegetative
development)
Gathering and ranking of selection criteria Group Identification & ranking
Ranking of preferred clones by plot Ranking
Harvest
Gathering and ranking of selection criteria Group Identification & ranking
Ranking of preferred clones by plot Ranking
Standard evaluation of yield Direct observation by counting and weighing
Post-Harvest
Organoleptic Evaluation By panel (women / men)Standard Evaluation Direct Observation
Gathering and ranking of selection criteria Group Identification & ranking
Ranking of the preferred clones Ranking
# = optional
DinajpurDinajpur DebigonjDebigonj
1st :Early Bulking: 732nd :Late Blight resistant: 623rd : Strong stem: 50
1st :Early Bulking: 942nd :Late Blight resistant: 643rd : Minimum use of fertilizer: 47
. .
Cone/VarietyRCBD
Trial Score Men (corn)
RCBD Trial Score
Women (beans)
Observation plot
Score Men (corn)
Observation plot
Score Women (beans)
Global ScoreOrder of ranking
CIP- 111 11 0 15 0 26 III
CIP-112 6 10 6 7 29 IICIP- 117 0 0 0 0 0 XIII
CIP-118 2 0 0 0 2 XII
CIP-120 2 0 7 0 9 IX
CIP- 126 13 13 12 11 49 ICIP- 131 2 0 11 0 13 VIII
CIP- 139 4 0 2 3 9 IX
LB-1 5 0 3 1 9 IX
LB-2 6 1 2 0 9 IX
LB-12 1 1 0 0 2 XII
LB-14 4 0 12 3 19 V
CIP- 2 2 0 0 1 3 XI
CIP-10 4 0 0 0 4 X
CIP-14 9 2 6 1 18 VI
Cardinal (CV) 7 1 5 1 14 VII
Diamant (CV) 12 2 9 2 25 IV
Total 90 30 90 30 240 -
Clonal selection at Debigonj
Clone/VarietyRCBD
Trial Score Men (corn)
RCBD Trial Score Women
(beans)
Observation plotScore Men (corn)
Observation plotScore Women
(beans)
Global Score
Order of ranking
CIP- 111 12 0 14 0 26 IIICIP-112 5 0 2 1 8 IXCIP- 117 0 0 0 0 0 XIVCIP-118 3 0 1 0 4 XI
CIP- 126 20 0 20 2 42 ICIP-120 2 0 9 0 11 VIIICIP- 131 3 0 20 0 23 IVCIP- 139 4 0 3 0 7 XLB-1 4 0 3 0 7 XLB-2 12 2 4 0 18 VILB-12 2 0 0 0 2 XIIILB-14 4 0 14 0 18 VICIP- 2 3 0 0 0 3 XIICIP-10 3 0 0 0 3 XIICIP-14 13 1 6 1 21 VCardinal (CV) 6 0 5 1 12 VII
Diamant (CV)
18 3 13 1 35 II
Total 114 6 114 6 240 -
Clonal selection at Dinajpur
DebigonjDebigonj
DinajpurDinajpur
Evaluation at Harvest stageGathering and Ranking of Criteria” (time of harvest)
Criteria Men (n=32)
Order if Importance
Women (n=28)
Order if Importance
Total (n=60)
Order if Importance
Score Score Score
Good yield 89 I 45 I 134 IGood storage 42 II 25 II 67 IIGood taste 20 III 30 III 50 IIIRed color 15 IV 22 V 37 VEarly variety 12 V 37 IV 49 IVGood Shape 7 VI 2 VII 9 VIIMedium size 4 VII 6 VI 10 VIDisease resistant 3 VIII 1 VIII 4 VIIITotal 192 - 168 - 360 -
Dinajpur
Criteria Men (n=33)
Order if Importance
Women (n=27)
Order if Importance
Total (n=60)
Order if Importance
Score Score Score
Good yield 70 I 12 I 82 IGood size (medium) 10 V 54 II 64 IIEarly variety 35 II 22 III 57 IIIDisease resistant 12 IV 42 V 54 IVGood marketing 2 VII 2 IX 4 IXGood storage 35 II 9 V 44 VGood taste 28 III 2 VI 30 VISticky nature 4 VI 14 VII 18 VIIShallow eyes 0 VIII 5 VIII 5 VIIIRed color 2 VII 0 X 2 X
Total 198 - 162 - 360 -
Debigonj
Rank of order
Reasons of selection Name of clones/varietiesRCBD Trial Observation plot
Dinajpur Debigonj Dinajpur Debigonj
I
Good yieldRed in color & uniformGood size & shape Good taste Good storage abilityEarly maturity Market friendlyDisease resistance
CIP-126 CIP-112 CIP-126 LB-12
IIGood yield Good size & shape Good storage ability Like as indigenous varietyRed in colorGood taste
LB-12 CIP-14 CIP-112 CIP-126
IIIGood yield Red in color Good size Like as indigenous varietyGood taste
CIP-112 CIP-111 CIP-139 CIP-112
Ranking of the Best Clones by Farmer (time of harvest)
FactorName of clones
First Second ThirdAppearance CIP-112 CIP-139 CIP117Taste CIP-112 CIP-139 CIP117Texture CIP-112 CIP-139 LB-12
Dinajpur
FactorName of clones/Varieties
First Second Third
Appearance CIP-112 Cardinal CIP-131
Taste CIP-112 LB-2 Cardinal
Texture CIP-112 CIP-139 CIP-131
Debigonj
Clones/Varieties Yield (t/ha)
Dinajpur Debigonj Average CIP- 139 47.03 52.00 49.52CIP- 131 55.43 59.48 57.46CIP- 126 55.93 57.70 56.82CIP-120 53.70 57.45 55.57CIP-118 51.87 57.42 54.65CIP- 117 51.71 55.01 53.36CIP-112 50.15 56.15 53.15LB-1 43.78 48.91 46.35LB-2 41.29 46.81 44.05LB-12 35.20 38.93 37.07LB-14 67.00 69.35 68.17CIP- 2 51.23 58.94 55.08CIP-10 56.61 61.26 58.93CIP-14 49.23 54.76 52.00CIP- 111 47.39 53.72 50.55Cardinal (CV) 43.10 47.00 45.05Diamant (CV) 41.48 41.70 41.59CV (%) 11.11 11.36LSD (0.05) 50.02 10.05
Yiel
d pe
rfor
man
ce
Criteria
Men (n=20)Order
if Importance
Women (n=20) Order
if Importance
Total (n=40) Order if
ImportanceScore Score Score
No color change after storage 19 III 12 V 31 IIINo hollow heart 0 8 VI 8 VII
No shrinkage after storage 1 VI 0 1 IX
Less sprouting 9 IV 16 IV 25 IV
No change to taste after storage 21 II 31 I 52 IILess weight loss 5 V 2 VII 7 VIII
Less rottage 55 I 28 II 83 INo black heart 9 IV 0 9 VI
Tight Skin 1 VI 23 III 24 V
TOTAL 120 - 120 - 240
Gathering and Ranking of Criteria(post-harvest)
DinajpurDinajpur
CriteriaMen
(n=32)Order if
ImportanceWomen (n=28)
Order if Importance
Total (n=60)
Order if Importance
Score Score Score
Not harmful to human health 9 VI 12 IV 21 V
No shrinkage after storage 7 VII 9 V 16 VII
No color change after storage 11 V 7 VIII 18 VI
Not easy to became soft after storage 1 IX 7 VIII 8 IX
Less rottage after storage 41 I 15 III 56 ILess sprouting 14 III 9 V 23 IV
No change to taste after storage 13 IV 36 I 49 IIStorage insect resistance 4 VIII 8 VI 12 VIII
Less storage disease or resistant 20 II 17 II 37 IIITOTAL 120 - 120 240 -
DebigonjDebigonj
Ranking of the Best Clones in Storage(post-harvest) at both locations
Clone/VarietyDinajpur Debigonj Global Score Order of
RankingMale Score
Female Score
Male ScoreFemale Score
CIP- 111 11 14 10 7 42 IVCIP-112 3 1 20 12 36 VICIP- 117 3 8 4 0 15 IXCIP-118 0 7 3 0 10 XIICIP-120 0 0 15 25 40 VCIP- 126 41 39 6 17 103 ICIP- 131 1 7 1 2 11 XICIP- 139 11 5 6 8 30 VIILB-1 6 5 7 2 20 VIIILB-2 4 3 2 0 9 XIIILB-12 26 10 8 13 57 IIILB-14 0 2 1 0 3 XIVCIP- 2 1 0 8 0 9 XIIICIP-10 2 0 4 8 14 XCIP-14 0 2 0 0 2 XVCardinal (CV) 11 16 20 23 70 II
Diamant (CV) 0 1 5 3 9 XIIITotal 90 30 90 30 - -
Standard Evaluation: Number of Sprouts, Tuber Weight and Health
(post-harvest)
Clone/varieties % Weight loss at 90 DAS % Rottage loss 90 DAS % Sprout 90 DAS Debigonj Dinajpur Debigonj Dinajpur Debigonj Dinajpur
CIP-111 1.96 1.04 0.00 3.13 90.00 100.00CIP-112 4.11 5.00 0.00 0.81 80.00 100.00CIP-117 2.17 9.18 0.00 0.00 100.00 100.00CIP-118 2.44 13.27 0.00 4.55 100.00 92.54CIP-120 1.96 3.12 0.00 5.75 60.00 96.59CIP-126 4.76 3.12 0.00 2.63 100.00 96.05CIP-131 3.61 7.14 10.00 6.56 100.00 98.36CIP-139 2.44 3.12 0.00 9.52 100.00 95.24CIP-2 4.00 1.09 0.00 1.49 60.00 100.00CIP-10 3.93 1.06 0.00 0.00 60.00 100.00CIP14 2.91 1.04 0.00 2.53 100.00 100.00LB-1 9.09 9.57 0.00 8.62 100.00 87.93LB-2 2.44 5.21 0.00 8.33 100.00 94.44LB-12 3.23 3.12 0.00 2.47 90.00 96.30LB-14 4.76 7.61 10.00 3.70 100.00 97.59Cardinal 3.61 7.45 0.00 1.19 100.00 98.81Diamant 4.76 9.38 0.00 1.54 100.00 100.00
.
Friedman test
Is a non- parametric statistical test.
It is used to detect differences in treatments across multiple test attempts.
The procedure involves ranking each row (or block) together, then considering the values of ranks by columns.
Applicable to complete block designs.
Statistical AnalysisFriedmanFriedman
Friedman test is a nonparametric analysis of a randomized block experiment.
It is used to detect differences in treatments across multiple test attempts.
ANOVAANOVA
ANOVA is a parametric analysis of a randomized block experiment
It is used to detect differences in treatments across multiple test attempts.
Kruskal-WallisKruskal-Wallis
Performs a nonparametric analysis of the one-way analysis of variance
The Kruskal-Wallis hypotheses are:
- H0: the population medians are all equal versus
- H1: the medians are not all equal
Statistical Analysis
Principal Components Principal Components
PCA is a data reduction technique used to identify a small set of variables that account for a large proportion of the total variance in the original variables.
Components can be calculated from the correlation matrix or the covariance matrix.
Output consists of the eigenvalues, the proportion and cumulative proportion of the total variance explained by each principal component, and the coefficients for each principal component.
Consolidation of evaluations: Principal Componentes
Local CodeInstitutional
numberClone or
variety name
CIP_111 CIP380583.8 Baseko
CIP_112 CIP380606.6 Enfula
CIP_117 CIP386292.3 IRA-92
CIP_118 CIP388615.22 C91.640
CIP_120 CIP389429.31 TS-15
CIP_126 CIP392797.22 UNICA
CIP_131 CIP393708.31 95.32
CIP_139 CIP396311.1 C95.276
LB_1 CIP391004.18
LB_2 CIP391046.14
LB_12 CIP396031.119
LB_14 CIP396244.12
CIP_2 CIP391846.5 LR-93.309
CIP_10 CIP397029.21 364.21
CIP_14 CIP397079.6 317.6
Cardinal Cardinal (CV)
Diamant Diamant (CV)
Consolidation of evaluations: Used weights and ranking
Local CodeInstitutional
numberClone or
variety name
GS_Veg_Debigonj
GS_Veg_Dinajpur
App_Debig
Taste_Debig
Texture_Debig
App_Dinaj
Taste_Dinaj
Texture_Dinaj
GS_Storage_Debig
GS_Storage_Dinaj
Yield_MT_Debig
Yield_MT_Dinaj
CIP_111 CIP380583.8 Baseko 26 26 95 145 184 35 33 60 17 25 53.717 47.39
CIP_112 CIP380606.6 Enfula 29 8 158 240 249 93 80 153 32 4 56.15 50.15
CIP_117 CIP386292.3 IRA-92 0 0 68 91 111 58 54 87 4 11 55.01 51.71
CIP_118 CIP388615.22 C91.640 2 4 64 118 135 39 33 72 3 7 57.42 51.87
CIP_120 CIP389429.31 TS-15 9 11 100 127 159 21 20 48 40 0 57.447 53.7
CIP_126 CIP392797.22 UNICA 49 42 87 112 168 56 38 57 23 80 57.703 55.93
CIP_131 CIP393708.31 95.32 13 23 136 186 243 34 24 72 3 8 59.48 55.43
CIP_139 CIP396311.1 C95.276 9 7 85 130 147 70 60 114 14 16 52 47.03
LB_1 CIP391004.18 9 7 59 116 108 52 43 96 9 11 48.91 43.78
LB_2 CIP391046.14 9 18 122 198 243 50 49 96 2 7 46.81 41.29
LB_12 CIP396031.119 2 2 76 140 156 39 35 51 21 36 38.93 35.2
LB_14 CIP396244.12 19 18 66 97 102 40 37 84 1 2 69.347 67
CIP_2 CIP391846.5 LR-93.309 3 3 120 173 216 26 17 39 8 1 58.937 51.23
CIP_10 CIP397029.21 364.21 4 3 77 113 150 35 31 63 12 2 61.257 56.61
CIP_14 CIP397079.6 317.6 18 21 87 100 123 26 23 45 0 2 54.76 49.23
Cardinal Cardinal (CV) 14 12 138 185 216 46 41 75 43 27 46.997 43.1
Diamant Diamant (CV) 25 35 107 127 180 54 46 84 8 1 41.703 41.48
From the studied there were six CIP clones (CIP-111, CIP-112, CIP-126, CIP-139, CIP-14 and LB-12) selected for next year trial.
Conclusion
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