experience in wpu mouselab revised
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Mara KettleRocheSummer 2012
Skills Learned• General Mouse Lab Procedure
and Skills-including husbandry• Experimental Preparation• Experimental Design• Data Collection and Analysis• Scientific Literature• Professional Social Skills
General Mouse Lab Procedure and Skills Proper Handling of Mice
Maintenance Sexing
Behavioral Tests Formalin Test Hot Plate Test Social Interaction
Experimental Preparation* Observed an on-going pain experiment
Formalin Pain Test and Social Interaction
Preliminary Literature Search Pubmed Academic Search Complete
(EBSCOhost.com) Helped Design an Experiment
Short Term Pain Study-Major Focus of my Work
Hypothesis
Stress decreases pain response because it triggers a pain suppression response called SIA
Necessary Components Aversive Stimuli: Stressors:
Restraint (severe) Noxious Pain Stimuli: Pain Test: Hot
Plate
What is SIA?
“a phenomenon of pain suppression that occurs upon exposure to stressful stimuli”1
Accidents
Wars/Battles
Trauma
Experimental Design
16 Male Mice: BTBR T+ tf/J (n=8) C57 BL/6J(n=8)
Restraint Stress
Hot Plate Test
Strains of Mice Used
BTBR C57•An autistic model•Show reduced social interactions, impaired play, low exploratory behavior, and high anxiety
• Used as a control/general purpose strain •Breeds well, long life-span
Experimental Design (cont.)1. Pre-stress pain test
2. Give mice stress Restraint stress for an hour in clear 50ml
centrifuge tubes
3. Post-stress pain test
Experimental Methods• Pre Stress Testing
• Cages were housed outside testing room
• Mice were placed onto hotplate heated 53°C and latency until first pain behavior was measured
• Pain latency was recorded and procedure was repeated until all mice were tested
Restraint Stress
• Method • 50ml centrifuge tubes
• 1 hour duration
• Post-stress testing began immediately following
• post stress pain latency time recorded
Results
P<0.05
P<0.05
Results cont…
Conclusion:
Restraint stress increases hotplate latencies in C57 and BTBR mice
It appears that there is a strain by treatment effect ;C57 mice demonstrate a greater stress induced analgesia”
References
1 Butler RK, Finn DP. Stress-induced analgesia. Prog Neurobiol. 2009 Jul;88(3):184-202. Epub 2009 Apr 22. Review. PubMed PMID: 19393288.
Acknowledgements
Roche ProgramWilliam Paterson UniversityDr. WeissMr. Norman SchanzDr. BennoAbbie Post (mentor) Joon Ho Seo (consultant)
Thank You
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