especificidade de enzimas de restrição eletroforese – padrão de digestão

Especificidade de enzimas de restrição

Eletroforese – padrão de digestão

Southern Blot

Southern Blot

Sequenciamento

Genoma completo

Em fase sólida

PCR

Estudos Forenses

Figure 6.10. DNA and Forensics. DNA analysis can be used to establish guilt in criminal cases. Here, DNA was isolated from bloodstains on the pants and shirt of a defendant and amplified by PCR. The DNA was then compared to the DNA from the victim as well as the defendant using gel electrophoresis and autoradiography. DNA from the bloodstains on the defendant's clothing matched the pattern of the victim, but not that of the defendant. The frequency of a coincidental match of the DNA pattern on the clothing and the victim is approximately 1 in 33 billion. Lanes λ, 1kb, and TS = Control DNA samples; lane D = DNA from the defendant; jeans = DNA isolated from bloodstains on defendent's pants; shirt = DNA isolated from bloodstains of the defendant's shirt (two different amounts analyzed); V = DNA sample from victim's blood.

Clonagem

Adaptadores

Plasmídios – mapa de restrição

Fagos como vetores de clonagem – empacotamento “in vitro”

M13 fago secretado

Biblioteca Genômica

Triagem

Sonda degenerada

YAC BAC