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DivisionofTeachingLabsPoliciesandProceduresWhenparticipatinginlaboratorysessionsintheDivisionofTeachingLabs(DTL)attheUniversityofToronto(UofT),FacultyofMedicine,thereareseveralpoliciesandbestpracticesthatmustbefollowed.HereatDTLweconstantlystrivetoprovidethesafestenvironmentpossible.Tothisendweaskthateachstudentreadthefollowingpoliciesandprocedures.KeepinmindthatasaUofTstudent,undertheCodeofStudentConduct,youareexpectedtoabidebythesepoliciesandprocedures.
PART ONE: GENERAL LAB POLICIES AND PROCEDURES
GENERALRULES1. Alwaysactinaresponsiblemannerinthelab.2. Alwaysbepreparedbyreadingthroughthelabinstructionspriortocomingtoclass.Alwayscheck-inwithyour
instructorbeforeproceedingwithanexperimentandifyouareunsureofanyofthedirectionsorhowtooperateparticularequipment.
3. Keepworkareascleanandtidyatalltimes.Likewisekeepthefloorandaislesclear.Bringonlythenecessarylaboratorymaterials(e.g.labmanualandnotebook)toyourbench.Otheritems(coats,backpacks,etc.)shouldbestoredinthedesignatedareasinthelabroom.
4. Alwaysrespectyourfellowstudentsandotherpersonnelinthelab.Tohelpreducethepossibilityofaccidentsyoushouldatalltimesbeconcentratingonwhatyouaredoing,andatnotimeshouldyouengageinanyformofhorseplay.
5. Neverconsumefoodorbeveragesorplaceanyfood/beverageitemsonanylabsurfacesincludingtheInterlabs.Neverdisposeoffoodorbeveragewasteinlabgarbagebins.Ifyouwishtoconsumefoodorbeveragesyoumaydosooutinthehallwaysanddisposeofthefood/beveragewasteinthehallwaygarbagebins.
6. Dressappropriatelyforalllabactivitiesbywearingclosed-toeshoesandlongpants.Alwaystiebacklong/loosehairandsecureanydanglingjewellery.
7. Weartheappropriatepersonalprotectiveequipment,PPE,(e.g.gloves,goggles,labcoat)asdirectedbyyourinstructor.Afullybuttoneduplabcoatisrequiredtoparticipateinallwetlabsessions.Eachstudentmustbringtheirownlabcoattothelab,whichcanbepurchasedfromtheBookstoreorMedStoresoncampus.
8. Keepyourhandsawayfromyourface,eyes,mouthandbody.Donothandleanyportabledevices,suchascellphones,laptops,orotherdeviceswithglovedorunwashedhands.
9. RemoveallPPEandwashyourhandswithsoapandwaterafterperformingexperimentsandbeforeexitingthelabroom.UsedesignatedhandwashingsinksONLYforwashinghands,notfordisposingofreagents,rinsingglasswareorconductingexperiments.
10. Cleanupafterthelabby:(a)disposingofwasteindesignatedcontainers;(b)removingalllabelsfromreusableglassware,thenrinsingtheglasswareandplacingitinthedesignatedcontainers;(c)clearingyourlabbenchandanyInterlabspacethatyou’veused;and(d)rinsingandreturningallequipmentcleanandinworkingordertotheiroriginalplace.
11. Ifanyequipmentisbrokenormalfunctioning,informyourinstructorortheInterlabtechniciansothatitcanberepaired.12. NEVERpipettebymouth;onlyusetherubberbulbsorpipetteaidsprovided.Whenusingapipetteaid,youmust
beextremelycarefultonotdrawanyliquidintothepipetteaid.13. Neverbringchemicals,biologicalorganisms,glasswareorequipmentintotheInterlabexceptwhennecessarytousea
pieceofequipmentlocatedthere(e.g.balance,spectrophotometer,centrifuge,etc.)ortousethefumehoodintheInterlab.IfitisnecessarytobringsuchitemsintotheInterlabthenthestudentmustalsobringitbacktohis/herlabbenchafteruse.
14. Nevertransferequipment,glassware,biologicalorganismsorchemicalsfromonelabtoanotherunlessspecifiedandsupervisedbytheinstructor.TheinstructorshouldinformtheInterlabtechnicianofanyequipmenttransfers.
15. Whenusingequipmenttakenoffshelvesorwagonsreturnthemtotheiroriginallocationattheendofthelabsession.16. Toreducethepossibilityofaccidents,keepInterlabtraffictoaminimumsothatitdoesnotbecomeovercrowded.17. Handleallglassware,sharps,organismsandchemicalsinarespectfulmanneranddisposeofthemproperly.Specific
guidelinesonhowtodisposeofvarioustypesofwastearedescribedinPartTwo.18. Letyourinstructorknowifyouhaveanyallergiesormedicalconditionswhichmayaffectyourabilitytoconductan
experimentortoreceivetreatmentintheeventofanaccidentorhealthincident.
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EMERGENCIES,ACCIDENTS&INJURIES19. Knowthelocationandoperatingprocedureoftheeyewashstationwithinyourlabandemergencyshowerinthe
interlab.Yourinstructorwillshowyouhowtouseaneyewashstation.20. Knowthelocationofthedustpanandbroom,spillkit,andfirstaidkit.YourTA/instructorwillattendtospills,broken
glasswareandfirstaid;studentsarenottocleanupspillsorattendtoinjuries.21. Reportanyaccident(spill,breakage,etc.),injury(cut,burn,etc.),orhealthincident(e.g.dizziness,fainting),tothe
instructorimmediately,nomatterhowtrivialitmayappear.22. Ifachemicalsplashesinyoureye(s),immediatelyflushwithrunningwaterfromtheeyewashstationforatleast20
minutes.Notifyyourinstructorimmediately.23. Ifachemicalsplashesonyourskin,immediatelyflushwithrunningwaterfromtheemergencyshowerforatleast20
minutes.Ifclothingiscontaminateditshouldberemoved.Notifyyourinstructorimmediately.Notethatuseoftheshowerisusuallyonlynecessaryifalargeamountofahazardoussubstancehassplashedonyourbody(e.g.ifyousplashasmallamountofchemicalonyourhand,youcanflushitwithwaterinthesinkratherthantheshower).
24. Knowthelocationofthefirealarmandemergencyexits.25. Incaseofemergencyorfiredrill,staycalmandfollowyourinstructor’sdirections.Youmayberequiredtoshutdown
certainequipment(e.g.Bunsenburners)beforeexitingthelabroom.Leaveallbags,coats,etc.inthelabroomsandproceedinanorderlyfashionalongwithyourinstructortothenearestexit.Yourinstructorwillensurethatthelabroomislocked.
WORKINGWITHOPENFLAMES&HEATINGSUBSTANCES26. Alwaysfollowyourinstructor’sdirectionsontheproperuseofBunsenburnersandotherheatingdevices(e.g.hotplates,
hightemperaturewaterbaths).27. ExerciseextremecautionwhenusingaBunsenburner.Tiebacklong/loosehair,loosejewellery,andensureyouarea
safedistancefromtheflameatalltimes.Donotputanysubstanceintotheflameunlessspecificallyinstructedtodoso.NEVERreachoveranexposedflame.NEVERleavealitBunsenburnerunattended.
28. NEVERleaveanythingthatisbeingheatedathightemperatureorisvisiblyreactingunattended.29. Alwaysturntheburnerorhotplateoffwhennotinuse.30. Exerciseextremecautionwhentransferringsubstancesintoandoutofboilingwaterbaths,heatingblocksor
microwaves.31. Protectyourhands(e.g.usetongs,wearprotectivegloves)whenhandlingglasswareormetalobjectsthathavebeen
heated.
HANDLINGREUSABLEGLASSWARE32. Studentsmustrespectglasswareaspotentiallyhazardousanddangerousitems.Glasswaremustonlybehandledforits
intendedpurpose.33. DirtyreusableglasswareMUSTbeplacedintheappropriatelymarkedDIRTYglasswarecontainers.DONOTPUTDIRTY
GLASSWAREBACKINWITHCLEANGLASSWARE.34. Thoroughlyrinseglassware(ifnon-hazardous)andremovealllabelsbeforeplacingtheminthedirtyglasswarebins.35. Brokenanddisposableglassware(includingPasteurpipettes),plasticconsumables(e.g.tips),anditemsdesignatedas
sharpsaretobedisposedofintheappropriatecontainersasdescribedinPartTwo.
RECYCLING36. Inmanyinstanceswastegeneratedwithinalaboratorysettingcanbesafelyrecycledtherebyminimizingthe
environmentalimpactofyourwork.Itemsthatmayberecycledinclude:glassware,plasticware,paperproducts,electronics(toner,computerequipment,labequipment),andbatteries
37. BeforeyouconsiderrecyclinganysolidwasteyoumustensurethatitisNOTCONTAMINATEDwithanyhazardousmaterial;ithastobefreeofanybiological,chemical,orradioactivecontamination.
38. Allproductsmustbetriplerinsedbeforetheyareplacedintothedesignatedrecyclingcontainer/bin.39. Pleasecheckwithyourinstructorastowhetheryoumayrecycleaparticularitem.
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PART TWO: PROPER HANDLING & WASTE DISPOSAL IN THE LAB
CHEMICALHANDLINGANDDISPOSAL
Duringthecourseofalabsessionyoumayberequiredtodispenseorhandlehazardousreagentsthatmaybereactive,poisonous,and/orcorrosive.PleasemakesurethatyouuseappropriatePPEandexerciseprecautionswhenhandlingsuchchemicalsanddisposeofthemintotheappropriatecontainerasoutlinedbelow.
Notethatglasswareandconsumablesthathavebiologicalorradioactivecontaminationaretreateddifferently(seesectionsonWorkingwithOrganismsandWorkingwithRadioisotopesbelow).
Whenworkingwithchemicals:• Payattentiontowarninglabelsprovidedonchemicalbottles.• Takeonlyasmuchchemicalasyouneed.• Neverreturnunusedchemicalstotheiroriginalcontainers.• Nevertouchortasteanychemicals.Whensmellingachemicalusethewaftingtechnique.Whendisposingofchemicalwaste:• LIQUIDCHEMICALS:
o Usedesignatedbottlesorbeakersthatwillbesetupandlabelledinthelab.DONOTfillcontainerspast75%ofthetotalvolume.
o Somechemicalscanbedisposedofdownthedrain;beforedoingso,verifywithyourinstructorthatthisispermitted.
• SOLIDCHEMICALS:o Usedesignatedbeakersthatwillbesetupandlabelledinthelab.
• LABCONSUMABLESUSEDWITHCHEMICALS(EXCEPTMEDICALSHARPS):o LabConsumables(LCs)suchastips,microtubes,Pasteurpipettes,thathavecomeintocontactwith
non-hazardouschemicalsarecollectedinaMilkshakeCupatyourworkbenchanddisposedattheendofyourclassinadesignatedcontainer.
o LCssuchasplastictubes,plastic96-wellplates,plasticsyringes(notneedles)thathavecomeintocontactwithnon-hazardouschemicalsshouldberinsedandcollectedinadesignatedcontaineronthecentrebench.Theseplasticswillberecycledwiththemunicipalwaste.
o LCsusedwithhazardousmaterialmustbedisposedofinaGREENPAIL,unlessdesignatedotherwisebyyourinstructor.
o NotethatPasteurpipettesarenotreused,thereforetheymustalwaysbedisposedofintheGREENPAIL.DonotplacePasteurpipettesintobasinsmarkedfordirtypipettes(thisreferstoreusableglasspipettesonly).Remembertoreturntherubberbulbtothegenerallaboratorysuppliestray.
• MEDICALSHARPSUSEDWITHCHEMICALS:o Razorblades,scalpels,needles,lancetsandothermedicalsharpsaredisposedofinaYELLOWBIOHAZARD
SHARPSCONTAINERONLY!
Ifyouareunsureorconfusedastowheresomethingshouldbedisposedofalwaysaskyourinstructor!Imagesofthevariousdisposalcontainerscanbefoundatthiswebsite:http://teachinglabs.med.utoronto.ca/StudentArea/Pages/DTL_LabPolicy.html
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WORKINGWITHANIMALSAMPLESOverview:AnimalworkistypicallyconductedintheDepartmentofComparativeMedicine’s(DCM)facilities.StudentswillreceivetrainingfromDCMandmustcomplywiththeirrulesandregulationsforproperanimalcare.ForanimalexperimentsconductedinDTL,studentswillreceivehands-ontrainingfromtheCourseCoordinator,teachingassistant,oraDCMstaffmember.ProceduresatDTL:AnimalproductsarecollectedinbiodegradableGREENBAGS.DONOTplaceanyothermaterialssuchasgloves,papertowels,tips,needles,etc.,inthesebags.Studentsareresponsibleforthesurgicalinstrumentsthattheyuse.Allsurgicalinstrumentsmustbesoakedandwashedaftereachusetopreventdamage.Instructionswillbeprovidedinthelab.Photographinganyanimalworkisnotpermissiblegiventhesensitivenaturesurroundingtheuseofanimalsinexperiments.
WORKINGWITHHUMANSAMPLESWorkingwithanddisposingofhumansamples:UniversalPrecautionsmustbetakenwheneverconductingexperimentsinvolvinghumanbloodandanyotherpotentiallyinfectiousmaterial(e.g.saliva,urine,etc.).Sincewecannotdeterminebyeyewhethersuchmaterialsmaybeinfectious,wemusttreatthemasiftheyareinfectedandhandleanddisposeofthemaspotentiallyinfectiousmaterials.ThesematerialsandallcontaminatedwastegeneratedduringtheseexperimentsaredisposedofinYELLOWBIOHAZARDPAILS.Inthecaseofanaccidentinvolvinghumanbloodorotherpotentiallyinfectiousmaterial:
• Ifyouarebleeding,notifyyourinstructorimmediately.Donotaskfellowclassmatesforassistancesothatweminimizetheriskofexposuretoothers.
• Ifyourinjuryallows,youmaybeaskedtohelpcleanupanycontaminatedareas.• Anyonewhoassistsaninjuredpartyshouldwearglovestoprotectthemselves.• AllcontaminatedareasmustbeproperlydisinfectedandinfectiouswastedisposedofintheYELLOW
BIOHAZARDPAILS.
WORKINGINACONTAINMENTLEVEL2ROOM
RoomsthatarezonedasContainmentLevel2(CL2)havespecialrules:
v Usersoftheseroomsmaynotbringpersonalitems(e.g.backpacks,coats,etc.)intotheserooms.Designatedstorageroomswillbeprovidedforyoutostoreyourpersonalbelongings.
v Labcoatsusedintheseroomsmuststayintheserooms.Attheendofthecourse,yourlabcoatwillbedisinfectedandwashed,andreturnedtoyou.
Currently,DTLrooms3282,3284,3377,3379,3381and3383aredesignatedasCL2(indicatedbysignsonthedoors).
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WORKINGWITHMICROORGANISMS:RISKGROUP1WhenworkingwithmicroorganismsdesignatedasRiskGroup1(RG1),specialprecautionsmustbetakentoensuretheworkspaceisusedandcleanedproperlyandthatallbiologicallycontaminatedwasteisproperlydisposedofintheappropriatecontainers.PleaseverifywithyourinstructorwhetherthebiologicalsyouwillbeusingaredesignatedasRG1orRG2.WhenworkingwithRG1organisms:
• WeartheappropriatePPEasdirectedbyyourinstructor• DisinfectyourworkbenchBEFOREandAFTERusewitheitherDettolor70%alcohol
WhendisposingofRG1waste:
• DisposeofRG1biologicallycontaminatedwasteintoappropriatecontainers,usingthefollowingrules:
o GLASSWARE
§ REUSABLEGLASSPIPETTES:Biologicallycontaminatedreusableglasspipettes(notPasteurpipettes)shouldbeplacedinMETALPANSORBLEACH-FILLEDJARSasinstructedbyyourinstructor
§ OTHERREUSABLEGLASSWARE:Otherbiologicallycontaminatedreusableglassware(e.g.beakers,flasks,etc.)shouldbeplacedinthedesignatedMETALBUCKETS.
o MEDIAPLATES:PlacesolidmediaplatesinthedesignatedMETALBUCKETS.
o LABCONSUMABLES
§ LABCONSUMABLES:Biologicallycontaminatedconsumables(e.g.tips,Pasteurpipettes,butNOTmedicalsharpssuchasneedlesandrazors)aredisposedofinareceptaclelinedwithaREDBIOHAZARDBAGORMETALCONTAINER
§ MEDICALSHARPS:Razorblades,scalpels,needles,lancetsandothersharpsaredisposedofinaYELLOWBIOHAZARDSHARPSCONTAINERONLY!DoNOTattempttoremoveaneedlefromasyringeorrecaptheneedle;placetheentireassemblyinthesharpscontainer.
o LIQUIDWASTE:Usedesignatedbottlesorbeakersthatwillbesetupandlabelledinthelab.DONOTfillcontainerspast75%ofthetotalvolume.
Whenusingbiosafetycabinets(BSCs)withRG1organisms:
• Disinfecttheworkspacebeforeandafteruse• Tidyup• Disposeofanymaterials(tips,pipettes,etc.)asdirectedbyyourinstructor
NotethatstandardBSCprocedureisforonlyoneusertoworkataBSCatatime.Forteachingpurposes,wemayallowtwostudentstoworksimultaneouslyatalargerBSCforRISKGROUP1WORKONLY.
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WORKINGWITHMICROORGANISMS:RISKGROUP2WhenworkingwithmicroorganismsdesignatedasRiskGroup2(RG2)specialprecautionsmustbetakentoensuretheworkspaceisusedandcleanedproperlyandthatallRG2wasteisproperlydisposedofintheappropriatecontainers.PleaseverifywithyourinstructorwhetherthebiologicalsyouwillbeusingaredesignatedasRG1orRG2.WhenworkingwithRG2organisms:
• WeartheappropriatePPEasdirectedbyyourinstructor• DisinfectyourworkbenchBEFOREandAFTERusewitheitherDettolor70%alcohol
WhendisposingofRG2waste:• ALLRG2WASTE:AllwastefromRG2organisms(plates,tips,labconsumables,etc.)MUSTBEdisposedof
inYELLOWBIOHAZARDPAILS.• MEDICALSHARPS:Razorblades,scalpels,needles,lancetsandothersharpsaredisposedofinaYELLOW
BIOHAZARDSHARPSCONTAINERONLY!DoNOTattempttoremoveaneedlefromasyringeorrecaptheneedle;placetheentireassemblyinthesharpscontainer.
Whenusingbiosafetycabinets(BSCs)withRG2organisms:
• Disinfecttheworkspacebeforeandafteruseusingthe70%alcoholprovidedinspraybottles• Tidyup• Disposeofanymaterials(tips,pipettes,etc.)asdirectedbyyourinstructorNotethatbiosafetyregulationsstipulatethatONLYONEUSERSHOULDWORKATABSCATATIMEWHENCONDUCTINGWORKWITHRISKGROUP2ORGANISMS.StudentsmustcomplywiththisregulationwhenworkingwithRiskGroup2organismsinourBSCs.
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WORKINGWITHRADIOISOTOPESNote:For2017-18,nocoursesruninDTLwillbeusingradioisotopes.Whenworkingwithradioactivesubstances:
• WearglovesandalabcoatatALLTIMES• ONLYworkoverbenchesthathavebeencoveredwithwhiteabsorbentpaper• ONLYuseequipment/toolsthathavebeenspecificallydesignatedforradioactivework,suchasMARKED
centrifuges,MARKEDshakers,MARKEDpipettors,etc.• Understandtheproperties,risksandproperhandlingproceduresassociatedwiththeparticularradioisotopes
youareusing;askyourinstructorifyouareatanytimeunsureabouttheproperprocedures.• Ifanyequipmentorworkspacebecomescontaminatedwitharadioactivesubstanceinformyourinstructor
immediatelysothatitcanberemovedfromuseanddecontaminated.• Ifanaccidentorinjuryoccurswhileworkingwitharadioactivesubstanceinformyourinstructorimmediatelyso
thattheappropriateassessmentandtreatmentcanbeperformed.
Whendisposingofradioactivesubstances:• DisposeofradioactivewasteintotheappropriatelyMARKEDcontainers:
o LIQUIDS:CarefullypourorpipetteradioactiveliquidsintotheappropriateCELITECONTAINER–notethatspecificcontainersareuseddependingonthehalf-lifeoftheradioisotope.Besurenottooverfillthesecontainers.Donotputanysolidwasteinthesecontainers.
o SMALLLABCONSUMABLES(E.G.TIPS,TUBES):Placeintheprovidedemptygloveboxesonthebenchasyouareworking.Whenyouhavecompletedyourwork(orwhentheboxisfull),sealthegloveboxwithtapeanddisposeofitintheYELLOWRADIOACTIVEWASTEBIN.
o OTHERDISPOSABLESOLIDS:Disposeofsolids(e.g.gloves,papertowels,etc.)intheYELLOWRADIOACTIVEWASTEBIN.
o GLASSWARE:Beforetheycanbeplacedinthedirtyglasswarebins,radioactively-contaminatedglasswaremustbethoroughlyrinsedusingDettolsolutionandallrinsesolutionsdisposedofasradioactiveliquidwasteintheappropriatecelitecontainer.DoNOTdisposeoftherinsesolutionsdownthedrain!
• Seepicturesofcontainersat:http://teachinglabs.med.utoronto.ca/StudentArea/Pages/DTL_LabPolicy.html)
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