cher ry pi ckin g gui de team cherry picker blake hondl amit mehta jon millin ryan pope
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CHER
RY PI
CKIN
G GUI
DE
Team Cherry PickerTeam Cherry Picker
Blake HondlBlake Hondl
Amit MehtaAmit Mehta
Jon MillinJon Millin
Ryan PopeRyan Pope
Team Small MoleculeTeam Small Molecule
Blake HondlBlake Hondl
Amit MehtaAmit Mehta
Jon MillinJon Millin
Ryan PopeRyan Pope
Client & AdvisorClient & Advisor
Noël R. Peters, M.S.Noël R. Peters, M.S.
Keck-UWCCC Keck-UWCCC
Small Molecule Screening Small Molecule Screening FacilityFacility
Professor Willis TompkinsProfessor Willis TompkinsDepartment of Biomedical Department of Biomedical
EngineeringEngineering
AbstractAbstract High throughput screening (HTS) involves High throughput screening (HTS) involves
secondary testing of chemicals that initially secondary testing of chemicals that initially show desired characteristicsshow desired characteristics– Done manually and prone to errorsDone manually and prone to errors
Goal is to design a device to guide a Goal is to design a device to guide a micropipette user to a single microtiter wellmicropipette user to a single microtiter well
The device will increase efficiency and reduce The device will increase efficiency and reduce user erroruser error
Device implementationsDevice implementations– Java ApplicationJava Application– LCD ScreenLCD Screen– User InterfaceUser Interface
Problem StatementProblem Statement Design a device to guide a Design a device to guide a
micropipette user in the transfer of micropipette user in the transfer of small volumes of compounds small volumes of compounds between 384-well microtiter platesbetween 384-well microtiter plates
The device will increase the The device will increase the efficiency of transfers and reduce efficiency of transfers and reduce user erroruser error
BackgroundBackground High Throughput High Throughput
ScreeningScreening– Used in cancer and Used in cancer and
RNA researchRNA research– Secondary testing Secondary testing
performed on the performed on the chemicals that chemicals that initially show initially show promisepromise
– Use of plate reader Use of plate reader to determine to determine potential “hits”potential “hits”
EnVison® Plate Reader
MotivationMotivation Secondary testing is typically done Secondary testing is typically done
manually, making it difficult and manually, making it difficult and prone to errorsprone to errors
Row and column identification is Row and column identification is necessarynecessary– Interior wells are difficult to locateInterior wells are difficult to locate
Errors lead to increased time costsErrors lead to increased time costs– Only step in the process that is prone to Only step in the process that is prone to
significant human errorsignificant human error
Current CompetitionCurrent Competition Matrix MemowellMatrix Memowell® ®
– 96 well pipetting 96 well pipetting aidaid
– Lights up 4 wells Lights up 4 wells when used with when used with 384-well plate384-well plate
– Costs approx. Costs approx. $1000$1000
Quadra Cherry Quadra Cherry PickerPicker– AutomatedAutomated– Costs $150,000Costs $150,000
http://www.matrixtechcorp.comhttp://www.tomtec.com
Design ConstraintsDesign Constraints Must interface with MicrosoftMust interface with Microsoft ExcelExcel and userand user Effectively guide the user to the appropriate Effectively guide the user to the appropriate
wellswells Minimize required bench spaceMinimize required bench space
– Dimensions smaller than 8.5” x 11” Dimensions smaller than 8.5” x 11” Withstand exposure to various chemicals and Withstand exposure to various chemicals and
reagentsreagents– Dimethyl SulfoxideDimethyl Sulfoxide
Lightweight (<10 lbs) and inexpensive Lightweight (<10 lbs) and inexpensive (<$1000)(<$1000)
Final DesignFinal Design Java ApplicationJava Application
– User input to locate wells in one or two User input to locate wells in one or two platesplates
– Excel input from spreadsheet (future Excel input from spreadsheet (future work)work)
LCD Screen to show output from Java LCD Screen to show output from Java Application Application – Lines (cross-hairs) displayed to identify Lines (cross-hairs) displayed to identify
wells to userwells to user
Final DesignFinal Design Protection of the LCD screenProtection of the LCD screen
– Hard acrylic sheet protects LCD screen Hard acrylic sheet protects LCD screen from pressure and possesses good optical from pressure and possesses good optical clarityclarity
– Polypropylene sheet to protect from the Polypropylene sheet to protect from the Dimethyl Sulfoxide bufferDimethyl Sulfoxide buffer
Maintaining the position of well plates Maintaining the position of well plates on LCD screenon LCD screen– Two sets of tracks guide the movement of Two sets of tracks guide the movement of
“arms” which will fix the plates in place“arms” which will fix the plates in place
Prototype ConstructionPrototype Construction Purchased Purchased
refurbished LCD refurbished LCD screenscreen– 10.4” Screen10.4” Screen– InverterInverter– Ribbon cableRibbon cable
Needs to be Needs to be refreshed at 60 Hzrefreshed at 60 Hz– Too fast for serial or Too fast for serial or
USB portUSB port
Prototype ConstructionPrototype Construction Acquired materialsAcquired materials
– Laptop: Samsung SENS 800Laptop: Samsung SENS 800– Aluminum sheet metalAluminum sheet metal– Polypropylene coverPolypropylene cover– Non-glare 1/8” acrylic sheetNon-glare 1/8” acrylic sheet
Detached screen from Detached screen from basebase– Purchased components to Purchased components to
extend screenextend screen 30 connector HD ribbon cable30 connector HD ribbon cable
ProgrammingProgramming User interfaceUser interface
– User may choose User may choose between:between: User input or User input or
MicrosoftMicrosoft ExcelExcel file file inputinput
Using one well plate Using one well plate or twoor two
Full screen displayFull screen display Displays Displays
rectangle for rectangle for definition of well definition of well plate boundariesplate boundaries
Displays Displays intersecting lines intersecting lines according to user according to user input, input, illuminating a illuminating a single wellsingle well
Well plate on LCD screen
Design AnalysisDesign Analysis AdvantagesAdvantages
– Ease of useEase of use– ManufacturingManufacturing– AdaptabilityAdaptability– CostCost– MarketabilityMarketability– Reduces errorsReduces errors– Increases Increases
productivityproductivity
DisadvantagesDisadvantages– Requires computer Requires computer
interface interface – Requires Requires
purchasing of purchasing of hardware as well as hardware as well as software.software.
– Software upgrades Software upgrades to enable to enable adaptabilityadaptability
Cost AnalysisCost Analysis Used Laptop from SWAPUsed Laptop from SWAP =$250.00 =$250.00 1/16” Aluminum Sheet (8 ft1/16” Aluminum Sheet (8 ft22)) =$33.36 =$33.36 Acrylic Sheet (2)Acrylic Sheet (2) =$ 3.90 =$ 3.90 Polypropylene coverPolypropylene cover =$ 0.20 =$ 0.20 Flat Ribbon Cable & ConnectorFlat Ribbon Cable & Connector =$16.71 =$16.71 Miscellaneous ComponentsMiscellaneous Components =$ 7.00=$ 7.00
Total=$311.17Total=$311.17 Cost to purchase a new LCD flat screen for a Cost to purchase a new LCD flat screen for a
computer $300 (Future Work)computer $300 (Future Work)
Future WorkFuture Work Implement additional programming codeImplement additional programming code
– Allow the program to take input from an ExcelAllow the program to take input from an Excel file file Conduct initial testing on laptop prototypeConduct initial testing on laptop prototype Create interface between prototype and PCCreate interface between prototype and PC Design an optimal structure for the prototype, Design an optimal structure for the prototype,
emphasizing ease of use for the useremphasizing ease of use for the user Confirm prototype’s resistance to chemical Confirm prototype’s resistance to chemical
solvents encountered in the labsolvents encountered in the lab
ReferencesReferences ““Digikey.” Digi-key Corporation. http://www.digikey.com. Retrieved on April 15, 2004.Digikey.” Digi-key Corporation. http://www.digikey.com. Retrieved on April 15, 2004. ““Java Excel API Tutorial”. http://www.andykhan.com/jexcelapi/tutorial.html.Java Excel API Tutorial”. http://www.andykhan.com/jexcelapi/tutorial.html. ““LCD Basics: Monitor Technology 101.” ViewSonic. LCD Basics: Monitor Technology 101.” ViewSonic.
http://www.viewsonic.com/monitoruniversity/lcdbasics.htm.http://www.viewsonic.com/monitoruniversity/lcdbasics.htm. ““LCD Video Controller.” Subassembly Product Guide. LCD Video Controller.” Subassembly Product Guide.
http://www.trans2000.com/manual/adboard_manual.pdf.http://www.trans2000.com/manual/adboard_manual.pdf. ““Matrix Memowell.” Matrix Technologies. http://www.matrixtechcorp.com. Retrieved Matrix Memowell.” Matrix Technologies. http://www.matrixtechcorp.com. Retrieved
on January 23, 2004.on January 23, 2004. Peters, Noël. Personal Interview. January 30, 2004.Peters, Noël. Personal Interview. January 30, 2004. ““Product Specification for LB104V03 Liquid Crystal Display.” Products Engineering Dept. Product Specification for LB104V03 Liquid Crystal Display.” Products Engineering Dept.
LG. Philips LCD Co., Ltd. http://www.jacoflatpanels.com/lcdpdfs/LB104V03- LG. Philips LCD Co., Ltd. http://www.jacoflatpanels.com/lcdpdfs/LB104V03-A1_CAS(Ver0.1).pdf.A1_CAS(Ver0.1).pdf.
““Quadra Cherry Picker.” Tomtec. http://www.tomtec.com. Retrieved on January 29, Quadra Cherry Picker.” Tomtec. http://www.tomtec.com. Retrieved on January 29, 2004. 2004.
““Samsung.” Samsung Electronics. http://www.samsungusa.com. Retrieved on April 22, Samsung.” Samsung Electronics. http://www.samsungusa.com. Retrieved on April 22, 2004.2004.
Schowalter, Jeff. Personal Interview. March 26, 2004.Schowalter, Jeff. Personal Interview. March 26, 2004. ““Small Molecule Screening Facility.” UW-CCC. Small Molecule Screening Facility.” UW-CCC.
http://www.medsch.wisc.edu/smsf/Index.htm. Retrieved on January 23, 2004. http://www.medsch.wisc.edu/smsf/Index.htm. Retrieved on January 23, 2004. Wu, Thomas C. “An Introduction to Object-Oriented Programming with Java (Third Wu, Thomas C. “An Introduction to Object-Oriented Programming with Java (Third
Edition)” and imported classes from JavaBook.Edition)” and imported classes from JavaBook.
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