blood collection & processing
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BLOOD
COLLECTION AND
PROCESSING
M KASTHURI BAI
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OBJECTIVE
After following this lecture, students must
be able to understand:
Processes involved in blood donation
center.
Important criteria in donor selections. The donor unit processing.
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LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history Physical examination
Phlebotomy
Adverse donor reactionDonor unit processing
Storage of donated blood
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Donation process
Donor area - attractive
- open at convenient hours
- comfortably ventilated
- understanding
- professional
- well trained personnel
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LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history Physical examination
Phlebotomy
Adverse donor reactionDonor unit processing
Storage of donated blood
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Registration
Information fully identify the donor
Details
Date & time donation Name and address
Contact no.
Gender
Age/ date of birth
Previous transfusion/ TTD
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LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history Physical examination
Phlebotomy
Adverse donor reactionDonor unit processing
Storage of donated blood
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Donor selection
Important in protecting safety of blood
supply
To obtain truthful & accurate medicalhistory to ensure safe transfusion
Protect donor confidentiality
Uniform donor history questionaire
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LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history Physical examination
Phlebotomy
Adverse donor reactionDonor unit processing
Storage of donated blood
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LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history Physical examination
Phlebotomy
Adverse donor reactionDonor unit processing
Storage of donated blood
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Physical examination
Donor should be in good health
Donor deferred if under influence of
alcohol or drugs or with obvious signs of
cold or other illness
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Physical examination
Determination of Hb and hct levels
Hb : 12.516.0 g/dl (female)
: 13.518.0 g/dl (male) Blood pressure
Systolic : < 180 mmHg
: < 100 mmHg
Pulse
Pulse rate should be between 50100
beats per minute
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Physical examination
Temperature
Orally should not exceed 37.5C (99.5 F)
Weight 50 kg (110lb ) or more450 mL of blood
< 50 kgnot more than 12% of blood volume
Formula : Body wt(lbs) x 450 = allowable amt
110 (lbs)
Skin lesionfree at venipuncture
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LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history Physical examination
Phlebotomy
Adverse donor reactionDonor unit processing
Storage of donated blood
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Phlebotomy
Bag selection
Identification
Selection of vein Phlebotomy & collection of samples
Care of donor
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Bag selection
FDA approved
Pyrogenfree, sterile, sufficientanticoagulant
According to need and amount ofcollection
Single / triple
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Identification
Assigning identification number to each
individual donation
Allows donation process to be tracedand tracked
Numeric or alphanumeric no should be
placed on donation record, primary bag,
all satelite bags and sample tubes
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Selection of vein
Both arm inspected for lesions that may
indicate IV drug use or signs of infection
Blood drawn from large, firm vein in theantecubital area of the arm
Tourniquet or BP set inflated to 40
mmHg
Aseptic technique must be used
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Phlebotomy and collection of
samples Final check to verify donors
identification
During collection blood mixed withanticoagulant
Amount collected monitored carefully so
total does not exceed 525 mL
PPE
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Care of donor
After removing needle from vein
Apply firm pressure with sterile gauze
Donor recline on bed of donor chair for few
minutes Allow to sit. Follow donor to refreshment
area
Post phlebotomy care
Eat and drink before leaving
Drink more fluid in next 4 hours
Avoid alcohol until you have eaten
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Care of donor
Do not smoke for 30 minutes
If bleeding persists, raise hand and apply pressure
Fainting/ dizziness occurlie down with head btwknees
If symptoms persists contact BDC
Resume all normal activity if asymptomatic
Remove bandage within few hours Maintain high fluid intake for approx 3 days
THANK DONOR & ENCOURAGE REPEAT DONATION
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LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history Physical examination
Phlebotomy
Adverse donor reactionDonor unit processing
Storage of donated blood
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Adverse donor reaction
Personnel should be trained in CPR
Syncope (fainting)
Caused by sight of blood
Symptom: weakness, sweating, dizziness,pallor. Unconcious
Deep breathing and hyperventilation
Fainting
Place donor on back with feet raised above head Loose tight clothing
Adequate airways
Aromatic spirits
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LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history Physical examination
Phlebotomy
Adverse donor reactionDonor unit processing
Storage of donated blood
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Donor unit processing
ABO & Rh grouping
Antibody screening
Screening of transfusion transmitted
diseases Syphilis
Hbs Ag
Anti HCV
Anti HIV
Anti HTLV I / II
CMV
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LECTURE CONTENT
Donation process
Registration
Donor selection
Medical history Physical examination
Phlebotomy
Adverse donor reactionDonor unit processing
Storage of donated blood
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Storage of donated blood
Blood unit Storage condition Storage duration
Red blood cell 14 C Max 42 days
Platelets RT 5 days on agitator
Fresh frozen plasma frozen 1 year
Cryoprecipitate frozen 1 year
Granulocyte RT Transfused within 24
hours
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Thank you
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