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Laboratory Head
Shinozaki Research Collaborative Group
Goal
Activities
Plants differ from animals in various ways and have inherent
capabilities that animals lack, such as plant-hormone-mediated
regulation, responses to various environmental stresses, light
regulation, and high-efficiency totipotency. Our research group
is studying the functions of plant genes using various mutants
(T-DNA/transposon insertional mutants and enhancer-tagged
mutants) of Arabidopsis thaliana. The group is interested in
plant genes involved in environmental stress response and
Analysis of plant genes using insertional mutants,
enhance-tagged mutants or FOX lines
Collection of plant full-length cDNAs
MembersChief Scientist, Director of Gene Discovery Research Group
Kazuo SHINOZAKI, Ph.D. (2005. 4~) (1989. 4~2005.3 Director of Plant Molecular Biology Laboratory)
Research Scientist
Taishi UMEZAWA, Ph.D. (2005. 4~)
Miki FUJITA, Ph.D. (2006. 4~) (2003.4~2006.3 CREST Research Scientist)
Takashi KUROMORI, Ph.D. (2005.4~) Fumiyoshi MYOUGA, Ph.D. (2005.4~)
Postdoctoral Researcher
Kaoru URANO, Ph. D. (2005. 4~)
Fuminori TAKAHASHI, Ph.D. (2007. 5~) (2004.4~2007.3 Junior Research Associate)
Special Postdoctral Researcher
Rie NISHIYAMA, Ph. D. (2006. 4~)
JSPS Research Fellow
Behnam Babak, Ph.D. (2007.10~)
Special Fixed Term Contract Employee
Saho MIZUKADO (2008.4~) (2006.4~2008.3 PSC Technical Staff)
Junior Research Associate
Masahide MIZOGUCHI (2007.4~)
Student Trainee
Masaki NAKAMURA (2005.4~2007.3)
1.
2.
tolerance, plant hormone response, seed development and
germination. The group aims to understand complex systems
involved in the regulation of gene expression cellular signal
transduction in response to environmental stimuli in higher
plants. The group also wants to contribute to the collection of
useful resources for basic biology and biotechnology through
their research and development.
Analysis of plant genes involved in environmental stress
response and tolerance, plant hormone response, seed
development and germination
Comprehensive analysis of plant stress response by the
“-omics” approach
3.
4.
Bioresource Research Collaborative Group
Kazuo SHINOZAKI, Ph.D.
Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 171 ―
Umezawa, Nishiyama, Ito, Babak, Mizukado, Kobayashi, Matsuo, Eto, UranoTakahashi, Fujita, Mizoguchi, Shinozaki, Shodai, Ozawa
Specific Aims
1. Phenome analysis of Arabidopsis genes using Ds transposon mutant collection
Using Ds-tagged Arabidopsis mutants with homozygous
knockout genes , we are analyzing gene funct ions
comprehensively. We have observed phenotypic changes of
the mutants and analyzed the function of disrupted genes.
In the course of the project, we have isolated a male sterile
mutant, male sterility1 (ms1) (Fig. 1). The MS1 gene encodes
a PHD-type transcriptional activator and regulates pleiotropic
steps such as pollen grain and tapetum development.
2. Soybean full-length cDNA projectRIKEN PSC is planning to collect large sets of full-length
cDNAs from various crops or trees. Our group constructed
a full-length enriched cDNA library from soybean, which
is the most important legume crop, as part of the project.
Approximately 40,000 cDNA clones were collected, and
Agency Staff
Hatsuyo SHODAI (2000.1~) Hiroko KOBAYASHI (2003.4~)
Kumiko OZAWA (2003.4~)
[Antibiotic Laboratory]
Senior Research Scientist
Takuya ITO, Ph.D. (2007. 4~)(2000.4~2007.3 Plant Molecular Biology Laboratory)
[Plant Molecular Biology Laboratory]
Research Scientist
Takeshi KATAGIRI, Ph.D. (2001.10~2007.3)
Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 172 ―
some of the cDNAs were sequenced and analyzed. We are
now preparing a database system for browsing our database of
soybean full-length cDNAs (Fig.2).
3. Miniscale FOX hunting systemRecently, we developed a novel system known as full-length
cDNA over-expressor (FOX) gene hunting, which involves
the random overexpression of a normalized. Arabidopsis full-
length cDNA library. Using a miniscale version of the system,
which focused on 41 stress-inducible transcription factors, we
identified AtbZIP60, a bZIP-type transcription factor, as a salt-
tolerant gene.
4. Functional analysis of plant genes involved in environmental stress response
Plants have a complex network of signal transduction and
gene expression through plant hormones in response to
various environmental stresses, such as dehydration, high
Figure 1. Transmission electron microscopic analysis of the ms1 mutant.MI: Microspore, TA: Tapetum.(A and C) Wild type. (B and D) ms1 mutant.(Compare A and B) In the ms1 mutant, microspores (arrows) and tapetum (arrow heads) became vacuolated, and finally, no mature pollen grains were formed.(Compare C and D) In the ms1 mutant, characteristic pollen wall structure, exine, was not formed.
Figure 2. Schematic diagram of Soybean cDNA Project
Soybean RNA mixture
Full-length cDNA library
Cloning
Sequencing for 5’/3’-ends
Clustering analysis
Full-length
sequence
Agillent
oligoarray
Molecular
markers etc.
salinity and low temperature. To increase plant productivity,
our research group is conducting gene hunting and functional
analysis in relation to environmental stress response.
5. Transgenic wheat project: Toward the practical use of stress tolerant crops (Fig. 3)
To generate stress-tolerant transgenic wheat, we constructed
transformation vectors containing useful Arabidopsis drought-
tolerant genes such as AtGolS2, NCED3, and SnRK2C. In
addition, we collected 38 wheat full-length genes orthologous
to Arabidopsis genes that have been reported to function in
drought stress. Based on the information from wheat EST
pools, full-length cDNA clones, and microarray analysis,
we are identifying the wheat constitutive or drought-stress-
inducible promoters.
6. Comprehensive analysis of ABA-regulated dehydration response utilizing metabolomics and transcriptomics in Arabidopsis
In plants, adaptation to stress is a complex biological process
involving global changes in gene expression and metabolite
composition. We carried out an integrated analysis of the
metabolome and transcriptome to reveal new molecular
mechanisms of dynamic metabolic networks in response to
dehydration using a knockout mutant of the NCED3 gene
involved in the dehydration-inducible biosynthesis of ABA.
Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 173 ―
Figure 3. Engineering environmental stress tolerant wheatWe search and select the stress-inducible promoters and functional genes for molecular breeding about drought tolerance. Based on these knowledge, we produce the stress tolerant wheat in the field.
Publications【Original Papers】 (*Peer reviewed journals)
Sakurai T., Satou M., Akiyama K., Iida K., Seki M.,
Kuromori T., Ito T., Konagaya A., Toyoda T., Shinozaki
K.: “RARGE: a large-scale database of RIKEN
Arabidopsis resources ranging from transcriptome to
phenome.” Nuc. Acid. Res. 33, D647-D650 (2005).*
Osakabe Y., Maruyama K., Seki M., Satou M., Shinozaki
K., Yamaguchi-Shinozaki K.: “An LRR receptor kinase,
RPK1, is a key membrane-bound regulator of abscisic
acid early signaling in Arabidopsis.” Plant Cell 17,
1105-1119 (2005).*
Urano K., Hobo T., Shinzoaki K.: “Arabidopsis ADC
genes involved in polyamine biosynthesis are essential
for seed development.” FEBS Lett. 579, 1557-1564
(2005).*
Katagiri T., Ishiyama K., Kato T., Tabata S., Kobayashi
M., Shinozaki K.: “An important role of phosphatidic
acid in ABA signaling during germination in Arabidopsis
thaliana.” Plant J. 43, 107-117 (2005).*
Yamada M., Morishita H., Urano K., Shinozaki N.,
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Yamaguchi-Shinozaki K., Shinozaki K., Yoshiba Y.:
“Effects of free proline accumulation in petunias under
drought stress.” J. Exp. Bot. 56. 1975-1981 (2005).*
Noutoshi Y., Ito T., Seki M., Nakashita H., Yoshida S.,
Marco Y., Shirasu K., Shinozaki K.: “A single amino acid
insetion in the WRKY domain of the Arabidopsis TIR-
NBS-LRR-WRKY type disease resistance protein SLH
(SENSITIVE TO LOW HUMIDITY1) causes activation
of defense responses and hypersensitive cell death.” Plant
J. 43, 873-888 (2005).*
Ito T., Motohashi R., Kuromori T., Noutoshi Y., Seki M.,
Kamiya A., Mizukado S., Sakurai T., Shinozaki K.: “A
resource of 5,814 dissociation transposon-tagged and
sequence-indexed lines of Arabidopsis transposed from
start loci on chromosome 5.” Plant Cell Physiol. 46,
1149-1153 (2005).*
Noutoshi Y., Ito T., Shinozaki K.: “ALBINO AND PALE
GREEN 10 encodes BBMII isomerase involved in
histidine biosynghesis in Arabidopsis thaliana.” Plant
Cell Physiol. 46, 1165-1172 (2005).*
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
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Kamei A., Seki M., Umezawa T., Ishida J., Satou M.,
Akiyama K., Zhu J-K., Shinozaki K.: “Analysis of gene
expression profiles in Arabidopsis salt overly sensitive
mutants sos2-1 and sos3-1.” Plant Cell Environ. 28,
1267-1275 (2005).*
Iida K., Seki M., Sakurai T., Satou M., Akiyama K.,
Toyoda T., Konagaya A., Shinozaki K: “RARTF:
database and tools for complete sets of Arabidopsis
transcription factors.” DNA Res. 12, 247-256 (2005).*
Oono Y., Seki M., Satou M., Iida K., Akiyama K., Sakurai
T., Fujita M., Yamaguchi-Shinozaki K., Shinozaki K.:
“Monitoring expression profiles of Arabidopsis genes
during cold acclimation and deacclimation using DNA
microarrays.” Funct. Integr. Genomics 6, 212-234
(2005).*
Fujita Y., Fujita M., Satoh R., Maruyama K., Parvez
MM., Seki M., Hiratsu K., Ohme-Takagi M., Shinozaki
K., Yamaguchi-Shinozaki K.: “AREB1 is a transcription
activator of novel ABRE-dependent ABA-signaling that
enhances drought stress tolerance in Arabidopsis.” Plant
Cell 17, 3470-3488 (2005).*
Nakashima K., Fujita Y., Katsura K., Maruyama K.,
Narusaka Y., Seki M., Shinozaki K., Yamaguchi-
Shinozaki K.: “Transcriptional Regulation of ABI3- and
ABA-responsive Genes Including RD29B and RD29A
in Seeds, Germinating Embryos, and Seedlings of
Arabidopsis.” Plant Mol. Biol. 60, 51-68 (2005).*
Nishimura N., Kitahata N., Seki M., Narusaka Y.,
Narusaka M., Kuromori T., Asami T., Shinozaki
K., Hirayama T.: “Analysis of ABA Hypersensitive
Germination2 revealed the pivotal functions of PARN
in stress response in Arabidopsis.” Plant J., 44, 972-984
(2005).*
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Ito Y., Katsura K., Maruyama K., Taji T., Kobayashi
M., Seki M., Shinozaki K. Yamaguchi-Shinozaki
K.:“Functional analysis of rice DREB1/CBF-type
transcription factors involved in cold-responsive gene
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expression in transgenic rice”, Plant Cell Physiol., 47,
141-153 (2006).*
Yoshida T., Nishimura N., Kitahata N., Kuromori T.,
Ito T., Asami T., Shinozaki K. Hirayama T.: “ABA
Hypersensitive Germination3 encodes a protein
phosphatase 2C (AtPP2CA) that strongly regulates
ABA signaling during germination among Arabidopsis
PP2Cs”, Plant Physiol., 140, 115-126 (2006).*
Umezawa T., Okamoto M., Kushiro T., Nambara E.,
Oono Y., Seki M., Kobayashi M., Koshiba T., Kamiya Y.
Shinozaki K.: “CYP707A3, a major ABA 8’-hydroxylase
involved in dehydration rehydration response in
Arabidopsis thaliana”, Plant J., 46, 171-182 (2006).*
Furihata T., Maruyama K., Fujita Y., Umezawa T.,
Yoshida R., Shinozaki K. Yamaguchi-Shinozaki K.:
“ABA-dependent multisite phosphorylation regulates the
activity of a transcription activation AREB1”, Proc. Natl.
Acad. Sci. USA., 103, 1988-1993 (2006).*
Yoshida R., Umezawa T., Mizoguchi T., Takahashi S.,
Takahashi F. Shinozaki K.: “The regulatory domain
of SRK2E/OST1/SnRK2.6 interacts with ABI1 and
integrates ABA and osmotic stress signals controlling
stomatal closure in Arabidopsis”, J. Bio. Chem., 281,
5310-5318 (2006).*
Yamasaki K., Kigawa T., Inoue M., Yamasaki T., Yabuki
T., Aoki M., Seki E., Matsuda T., Tomo Y., Terada
T., Shirouzu M., Tanaka A., Seki M., Shinozaki K.
Yokoyama S.: “An Arabidopsis SBP-domain fragment
with a disrupted C-terminal zinc-binding site retains its
tertiary structure”, FEBS Lett., 580, 2109-2116 (2006).*
Hasegawa Y., Seki M., Mochizuki Y., Heida N., Hirosawa
K., Okamoto N., Sakurai T., Satou M., Akiyama K.,
Iida K., Lee K., Kanaya S., Demura T., Shinozaki K.,
Konagaya A. Toyoda T.: “A flexible representation of
omic knowledge for thorough analysis of microarray
data”, Plant methods, 2,5 (2006).*
Sakuma Y., Maruyama K., Osakabe Y., Qin F., Seki M.,
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
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Shinozaki K. Yamaguchi-Shinozaki K.:“Functional
analysis of an Arabidopsis transcription factor, DREB2A,
involved in drought-responsive gene expression”, Plant
Cell, 18,1292-1309 (2006).*
Noutoshi Y., Kuromori T., Wada T., Hirayama T., Kamiya
A., Imura Y., Yasuda M., Nakashita H., Shirasu K.
Shinzoaki K.:“Loss of NECROTIC SPOTTED LESIONS
1 associates with cell death and defense responses in
Arabidopsis thaliana”, Plant Mol. Biol., 62, 29-42
(2006).*
Kuromori T., Wada T., Kamiya A., Yuguchi M., Yokouchi
T., Imura Y., Takabe H., Sakurai T., Akiyama K.,
Hirayama T., Okada K. Shinzoaki K.:“A trial of phenome
analysis using 4000 Ds-insertional mutants in gene-
coding regions of Arabidopsis”, Plant J., 47, 640-651
(2006).*
Kitahata N., Han S-Y., Noji N., Saito T., Kobayashi
M., Nakano T., Kuchitsu K., Shinozaki K., Yoshida
S., Matsumoto S., Tsujimoto M. Asami T.:“A 9-cis-
epoxycarotenoid dioxygenase inhibitor for use in
the elucidation of abscisic acid action mechanisms”,
Bioorganic & Medicinal Chem., 15, 5555-5561 (2006).*
Nishiuchi T., Masuda D., Nakashita H., Ichimura K.,
Shinozaki K., Yoshida S., Kimura M., Yamaguchi I.
Yamaguchi K.:“Fusarium phytotoxin trichothecenes
have an elicitor-like activity in Arabidopsis thaliana, but
the activity differed significantly among their molecular
species”, Mol Plant Microve Interact., 19, 512-520
(2006).*
Machida M., Takechi K., Sato H., Chung S.J., Kuroiwa
H., Takio S., Seki M., Shinozaki K., Fujita T., Hasebe
M. Takano H.:“Gene for the peptidoglycan synthesis
pathway are essential for chloroplast division in moss”,
Proc. Natl. Acad. Sci. USA., 103, 6753-6758 (2006).*
Gross J., Cho W-K., Lezhneva L., Falk J., Krupinska
K., Shinzoaki K., Seki M., Herrmann R.G. Meurer J.:
“A plant locus essential for phylloquinone (Vitamin K1)
Biosynthesis orininated from a fusion of foure eubacterial
genes”, J. Biol. Chem., 281, 17189-17196 (2006).*
Oono Y., Seki M., Satou M., Iida K., Akiyama K.,
Sakurai T., Fujita M., Yamaguchi-Shinozaki K. Shinozaki
K.:“Monitoring expression profiles of Arabidopsis
genes during cold acclimation and deacclimation using
DNA microarrays”, Funct. Integr. Genomics, 6, 212-234
(2006).*
Myouga F., Motohashi R., Kuromori T., Nagata
N. Shinozaki K.:“An Arabidopsis chloroplast-
targeted Hsp101 homolog, APG6, has an essential
role in choloroplast development as well as heat stress
response”, Plant J., 48, 249-260 (2006).*
Sakuma Y., Maruyama K., Qin F., Osakabe Y., Shinozaki
K. Yamaguchi-Shinozaki K.: “Dual function of an
Arabidopsis transcription factor DREB2A in water-stress-
responsive and heat-stress-responsive gene expression”,
Proc. Natl. Acad. Sci. USA., 103. 18822-18827 (2006).*
Ichimura K., Casais C., Peck SC., Shinozaki K. Shirasu
K.:“MEKK1 is required for MPK4 activation and
regulates tissue specific and temperature dependent cell
death in Arabidopsis”, J. Bio. Chem., 281, 36969-36976
(2006).*
Ichikawa T., Nakazawa M., Kawashima M., Iizumi H.,
Kuroda H., Kondou Y., Tsuhara Y., Suzuki K., Ishikawa
A., Seki M., Fujita M., Motohashi R., Nagata N., Takagi
T., Shinozaki K. Matsui M.:“The FOX hunting system:
an alternative gain-of-function gene hunting technique”,
Plant J., 45, 974-986 (2006).*
Matsuda T., Kigawa T., Koshiba S., Inoue M., Aoki M.,
Yamasaki K., Seki M., Shinozaki K. Yokoyama S.: “Cell-
free synthesis of zinc-binding proteins”, J. Struct Funct
Genomics, 7, 93-100 (2006).*
Tran P. L-S., Nakashima K., Sakuma Y., Fujita Y.,
Osakabe Y., Qin F., Simpson SD., Maruyama K., Fujita Y.,
Shinozaki K. Yamaguchi-Shinozaki K.: “Co-expression
of the stress-inducible zinc finger homeodomain ZFHD1
and NAC transcription factors enhances exporession
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
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of the ERD1 gene in Arabidopsis”, Plant J., 49, 46-63
(2007).*
Qin F., Kakimoto M., Sakuma Y., Maruyama K., Osakabe
Y., Tran L-S., Shinzoaki K. Yamaguch-Shinozaki K.:
“Regulation and functional analysis of ZmDREB2A in
response to drought and heat stresses in Zea mays L.”,
Plant J.,50, 54-69 (2007).*
Mitsuda N.,Iwase A., Yamamoto H., Yoshida M., Seki
M., Shinozaki K. Ohme-Takagi M.: “NAC transcription
factors, NST1 and NST3, are key regulators of the
formation of secondary walls in woody tissues of
Arabidopsis”, Plant Cell, 19, 270-280 (2007).*
Takahashi F., Yoshida R., Ichimura K., Mizoguchi T., Seo
S., Yonezawa M., Maruyama K., Yamaguchi-Shinozaki
K. Shinozaki K.: “Dissecting the jasmonate signaling
pathway by a novel MAP kinase cascade, MKK3-MPK6,
in Arabidopsis”, Plant Cell, 19, 805-818 (2007).*
Itoh T., Tanaka, T., Barrero R.A., Yamasaki C., Fujii
Y., Hilton P.B., Antonio B.A., Aono H., Apweiler R.,
Bruskiewich R., Bureau T., Burr F., Fuks G., Habara T.,
Haberer G., Han B., Harada E., Hiraki A.T., Hirochika
H., Hoen D., Hokari H., Hosokawa S., Hsing Y.I., Ikawa
H., Ikeo K., Imanishi T., Ito Y., Jaiswal P., Kanno M.,
Kawahara Y., Kawamura T., Kawashima H., Khurana
J.P., Kikuchi S., , Komatsu S., Koyanagi K.O., Kubooka
H., Lieberherr D., Lin Y.C., Lonsdale D., Matsumoto
T., Matsuya A., McCombie W.R., Messing J., Miyao A.,
Mulder N., Nagamura Y., Nam J., Namiki N., Numa H.,
Nurimoto S., O'Donovan C., Ohyanagi H., Okido T., de
Oliveira A.C., Oota S., Osato N., Palmer L.E., Quetier
F., Raghuvanshi S.,Saichi N.,Sakai H., Sakai Y., Sakata
K., Sakurai T., Sato F., Sato Y., Schoof H., Seki M.,
Shibata M., Shimizu Y., Shinozaki K., Shinso Y., Singh
N.K., Smith-White B., Takeda J., Tanino M., Tatusova
T., Thongjuea S., Todokoro F., Tsugane M., Tyagi A.K.,
Vanavichit A., Wang A., Wing R.A., Yamaguchi K.,
Yamamoto M., Yamamoto N., Yu Y., Zhang H., Zhao
Q., Higo K., Burr B., Gojobori T. Sasaki T.: “Curated
Genome Annotation of Oryza sativa ssp. Japonica
and Comparative Genome Analysis with Arabidopsis
thaliana”. Genome Res. 17, 175-183 (2007).*
Nakagawa Y., Katagiri T., Shinozaki K., Qi Z., Tatsumi
H., Furuichi T., Kishigami A., Sokabe M., Kojima
I., Sato S., Kato T., Tabata S., Iida K., Terashima A.,
Nakano M., Ikeda M., Yamanaka T. Iida K.: “Arabidopsis
plasma membrane protein crucial for Ca2+ influx and
touch sensing in roots”, Proc. Natl. Acad. Sci. USA., 104,
3639-3644 (2007).*
Nakashima K., Tran P. L-S., Nguyen D.V., Fujita M.,
Maruyama K., Todaka D., Ito Y., Hayashi N., Shinozaki
K. Yamaguchi-Shinozaki K.: “Functional analysis of
a NAC-type transcription factor OsNAC6 involved in
abiotic and biotic stress-responsive gene expression in
rice”, Plant J., 51, 617-630 (2007).*
Mizuno S., Osakabe Y., Maruyama K., Ito T., Osakabe
K., Sato T., Shinozaki K. Yamaguchi-Shinozaki K.:
“Receptor-like protein kinase 2 (RPK 2) is a novel factor
controlling anther development in Arabidopsis thaliana”,
Plant J., 50, 751-766 (2007).*
Iuchi S., Koyama H., Iuchi A., Kobayashi Y., Kitabayashi
S., Kobayashi Y., Ikka T., Hirayama T., Shinozaki K.
Kobayashi M.: “Zinc finger protein STOP1 is critical
for proton tolerance in Arabidopsis and coregulates a
key gene in aluminum tolerance”, Proc. Natl. Acad. Sci.
USA., 104, 9900-9905 (2007).*
Okuda K., Myouga F., Motohashi R., Shinozaki
K. Shikanai T.: “Conserved domain structure of
pentatricopeptide repeat proteins involved in choroplast
RNA editing”, Proc. Natl. Acad. Sci. USA., 104,
8178-8183 (2007).*
Tian C., Chikayama E., Tsuboi Y., Kuromori T.,
Shinozaki K., Kikuchi J. Hirayama T.: “Top-down
phenomics of Arabidopsis thaliana-METABOLIC
PROFILING BY ONE- AND TWO-DIMENSIONAL
NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
AND TRANSCRIPTOME ANALYSIS OF ALBINO
MUTANTS”, J. Bio Chem., 282, 18532-18541 (2007).*
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
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Nishimura N., Yoshida T., Kitahata N., Asami T.,
Shinozaki K. Hirayama T.: “ABA-Hypersensitive
Germination1 encodes a protein phosphatase 2C,
an essential component of abscisic acid signaling in
Arabidopsis seed”, Plant J., 50, 935-949 (2007).*
Motohashi R., Yamazaki T., Myouga F., Ito T., Ito
K., Satou M., Kobayashi M., Nagata N., Yoshida S.,
Nagashima A., Tanaka K., Takahashi S. Shinozaki K.:
“Chloroplast ribosome release factor 1 (AtcpRF1) is
essential for chloroplast development”, Plant Mol. Biol.,
64, 481-497 (2007).*
Fujita M, Mizukado S, Fujita Y, Ichikawa T, Nakazawa
M, Seki M, Matsui M, Yamaguchi-Shinozaki K,
Shinozaki K.: “Identification of stress-tolerance-related
transcription-factor genes via mini-scale Full-length
cDNA over-eXpressor (FOX) gene hunting system”,
Biochem. Biophys.Res.Commun. 364, 250-257 (2007).*
Ukitsu H.*, Kuromori T.*, Toyooka K., Goto Y.,
Matsuoka K., Sakuradani E., Shimizu S., Kamiya
A., Imura Y., Yuguchi M., Wada T., Hirayama T.,
Shinozaki K. (* equally contribution) “Cytological and
Biochemical Analysis of COF1, an Arabidopsis Mutant
of an ABC Transporter Gene”. Plant Cell Physiol., 48,
1524-1533 (2007).*
Ito T., Nagata N., Yoshiba Y., Ohme-Takagi M., Ma H.
Shinozaki K.: “The Arabidopsis MALE STERILITY1
gene encoding a PHD-type transcription factor regulates
pollen and tapetum development”, Plant Cell, 19,
3549-3562 (2007).*
Miwa K., Takano J., Omori H., Seki M., Shinozaki
K. Fujiwara T.: “Plants tolerant of high boron levels”,
Science, 318, 1417 (2007).*
Tran L-S. P., Urao T., Qin F., Maruyama K., Kakimoto
T., Shinozaki K. Yamaguchi-Shinozaki K.: “Functional
analysis of AHK1/ATHK1 and cytokinin receptor
histidine kinases in response to abscisic acid, drought,
and salt stress in Arabidopsis”, Proc. Natl. Acad. Sci.
USA., 104, 20623-20628 (2007).*
Sakurai T., Plata G., Rodriguez-Zapata F., Seki M.,
Salcedo A., Toyoda A., Tohme J., Sasaki Y., Shinozaki K.
Ishitani M.: “Sequencing analysis of 20,000 full-length
cDNA clones from cassava reveals lineage specific
expansions in gene families related to stress response”,
BMC Plant Biology, 7, 66-84 (2007).*
Nanjo T., Sakurai T., Totoki Y., Toyoda A., Nishiguchi
M., Kado T., Igasaki T., Futamura N., Seki M., Sasaki
Y., Shinozaki K. Shinohara K.: “Functional annotation
of 19,841 Populus nigra full-length enriched cDNA
clones”, BMC Genomics, 8, 448-447 (2007).*
Sasaki Y., Takahashi K., Oono Y., Seki M., Yoshida R.,
Shinozaki K. Uemura M.:” Characterization of growth-
phase-specific responses to cold in Arabidopsis thaliana
suspension-cultured cells”, Plant Cell Environ., 31,
354-365 (2008).*
Sakai T., vd Honing H., Nishioka M., Uehara Y.,
Takahashi M., Fujisawa N., Saji K., Seki M., Shinozaki
K., Jones MA., Smirnoff N., Okada K. Wasteneys
GO: “Armadillo repeat-containing kinesins and a
NIMA-related kinase are required for epidermal-cell
morphogenesis in Arabidopsis”, Plant J., 53, 157-171
(2008).*
Garcia M., Myouga F., Takechi K., Sato H., Nabeshima
K., Nagata N., Takio S., Shinzoaki K. Takano H.: “An
Arabidopsis homolog of the bacterial peptidoglycan
synthesis enzyme MurE has an essential role in
chloroplast development”, Plant J., 53, 924-934 (2008).*
Yoshida T., Sakuma Y., Todaka D., Maruyama K.,
Qin F., Mizoi J., Kidokoro S., Fujita Y., Shinozaki K.
Yamaguchi- Shinozaki K.: “Functional analysis of an
Arabidopsis heat-shock transcription factor HsfA3 in
the transcriptional cascade downstream of the DREB2A
stress-regulatory system”, Biochem. Biophys. Res.
Commun., 368, 515-521 (2008).*
Kuromori T., Azumi Y., Hayakawa S., Kamiya A., Imura
Y., Wada T. Shinozaki K.: “Homologous chromosome
46.
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 178 ―
【Reviews】Seki M., Ishida J., Nakajima M., Enju A., Iida K., Satou
M., Fujita M., Narusaka Y., Narusaka M., Sakurai T.,
Akiyama K., Oono Y., Kamei A., Umezawa T., Mizukado
S., Maruyama K., Yamaguchi-Shinozaki K., Shinozaki
K.: “Genomic analysis of stress response.” In Plant
Abiotic Stress. M.A. Jenks and P.M. Hasegawa, eds,
Blackwell Publishing Ltd, Sheffield, UK, 248-265 (2005).
Seki M., Satou M., Sakurai T., Akiyama K., Iida K.,
Ishida J., Nakajima M., Enju A., Narusaka M., Fujita M.,
Oono Y., Yamaguchi-Shinozaki K., Carninci P., Kawai
J., Hayashizaki Y., Shinzoaki K.: “Full-length cDNAs
for the discovery and annotation of genes in Arabidopsis
thaliana.” Plant Functional Genomics, Dario Leister eds,
Food Products Press, 3-22 (2005).
Seki M., Ishida J., Nakajima M., Enju A., Kamei A.,
Oono Y., Narusaka M., Satou M., Sakurai T., Shinozaki
K.: “Gene expression profiling in plants using cDNA
microarrays.” DNA Microarrays, Edited by Ulrike A
Nuber, Taylor & Francis, Germany, 25-38 (2005).
Yamaguchi-Shinozaki K., Shinozaki K.: “Organization
of cis-acting regulatory elements in osmotic- and cold-
stress-responsive promoters.” Trends Plant Sci. 10,
88-94. (2005).*
Yamaguchi-Shinozaki K., Shinozaki K.: “Transcriptional
regulatory networks in cellular responses and tolerance to
dehydration and cold stresses.” Annual Review of Plant
Biology, 57, 781-803 (2006).
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pairing is completed in crossover defective atzip4
mutant”, Biochem. Biophys.Res.Commun., 370, 98-103
(2008).*
Cartagena Joyce A., Matsunaga S., Seki M., Kurihara
D., Yokoyama M., Shinozaki K., Fujimoto S., Azumi
Y., Uchimiya S. Fukui K.: “The Arabidopsis SDG4
contributes to the regulation of pollen tube growth by
methylation of histone H3 lysines 4 and 36 in muture
pollen”, Dev. Biol., 315, 355-368 (2008).*
Shimizu H., Peng L., Myouga F., Motohashi R.,
Shinozaki K. Shikanai T.: “CRR23/NdhL is a subunit
of the chloroplast NAD(P)H dehydrogenase complex in
Arabidopsis”, Plant Cell Physiol., 49, 835-842 (2008).*
Qin F., Sakuma Y., Tran L-S., Maruyama K., Kidokoro S.,
Fujita Y., Fujita M., Umezawa T., Sawano Y., Miyazono
K., Tanokura M., Shinozaki K. Yamaguchi-Shinozaki K.:
“Arabidopsis DREB2A-interacting proteins function as
RING E3 ligases and negatively regulate plant drought
stress-responsive gene expression”, Plant Cell, 20,
1693-1707 (2008).*
Mochida K., Saisho D., Yoshida T., Sakurai T. Shinozaki
K.: “TriMEDB: A database to integrate transcribed
markers and facilitate genetic studies of the tribe
Triticeae”, BMC Plant Biol., 8, 72 (2008)
Yasuda M., Ishikawa A., Jikumaru Y., Seki M., Umezawa
T., Asami T., Maruyama-Nakashita A., Kudo T.,
Shinozaki K., Yoshida S. Nakashita H.: “Antagonistic
interaction between syntemic acquired resistance and
the abscisic acid-mediated abiotic stress response in
Arabidopsis”, Plant Cell, 20, 1678-1692 (2008).*
Goda H., Sasaki E., Akiyama K., Maruyama-Nakashita
A., Nakabayashi K., Li W., Ogawa M., Yamauchi Y.,
Prestpm J., Aoki K., Kiba T., Takatsuto S., Fujioka S.,
Asami T., Nakano T., Kato H., Mizuno T., Sakakibara
H., Yamaguchi S., Nambara E., Kamiya Y., Takahashi
H., Yokota Hirai M., Sakurai T., Shinozaki K., Saito K.,
Yoshida S. Shimada Y.: “The AtGenExpress hormone-
and chemical-treatment data set: Experimental design,
data evaluation, model data analysis, and data access”,
Plant J., 55, 526-542 (2008).*
Endo A., Sawada Y., Takahashi H., Okamoto M., Ikegami
K., Koiwai H., Seo M., Toyomasu T., Mitsuhashi W.,
Shinozaki K., Nakazono M., Kamiya Y., Koshiba
T. Nambara E.: “Drought induction of Arabidopsis
9-cis-epoxycarotenoid dioxygenase occurs in vascular
parenchyma cells”, Plant Physiol., 147, 1984-1993
(2008).*
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 179 ―
Umezawa T., Fujita M., Fujita Y., Yamaguchi-Shinozaki
K., Shinozaki K.: “Enginieering drought tolerance in
plants: discovering and tailoring genes to unlock the
future.” Curr.Opin.Biotech., 17, 113-122 (2006).*
Shinozaki K., Yamaguchi-Shinozaki K.: “Global
analysis of gene networks to solve complex abiotic
stress responses, Cold Hardiness in Plants; Molecular
Genetics, Cell Biology and Physiology.” Proceeding
of 7th Internationa Plant Cold Hardiness Seminar in
Sapporo Japan, Edited by T.H.H. Chen, M. Uemura and S.
Fujikawa, CABI Publishing, 1-10 (2006).
Taji T., Takahashi S., Shinozaki K.: “Inositols and their
metabolites in abiotic and biotic stress responses.”
Subcellular Biochemistry Vol 39 on Biology of Inositols
and Phosphoinositides, Edetid by Arun Lahiri Majumder
and B.B. Biswas, 239-264 (2006).
Seki M., Kamiya A., Carninci P., Hayashizaki Y.,
Shinozaki K.: “Generation of full-length cDNA libraries
from plant materials.” In “Methods in Molecular
Biology-ESTs (Edited by Dr. John Parkinson).” Humana
Press Inc., NJ.in press (2006).
Fujita M., Fujita Y., Noutoshi Y., Takahashi F., Narusaka
Y., Yamaguchi-Shinzoaki K., Shinozaki K.: “Crosstalk
between abiotic and biotic stress responses: a current
view from the points of convergence in the stress
signaling networks.” Curr.Opin. Plant Biol. 9, 436-442
(2006).*
Yamaguchi-Shinozaki K., Sakuma Y., Ito Y., Shinozaki
K.: “The DRE/DREB Regulon of gene expression in
Arabidopsis and rice in response to drought and cold
stress.” Drought Adaptation in Cereals, Edted by Jean-
Marcel Ribaut, Food Products Press, 583-597 (2006).
Seki, M., Umezawa, T., Kim, J.M., Matsui, A., To,
T., Shinozaki, K.: “Transcriptome analysis of plant
drought and salt stress response., Advances in Molecular
Breeding toward Drought and Salt Tolerant Crops.”
Edited by Drs. M.A. Jenks, P.M. Hasegawa and S.M.
Jain., Springer Publishing. 261-283 (2007).
Nakashima K., Todaka D., Ahmed S., Umezawa T., Seki
M., Shinozaki K., Yamaguchi-Shinozaki K.: “Molecular
analysis of abiotic stress: Tolerant mechanisms in
soybean and its application to breeding.” JIRCAS
Working Report, 51, 13-19 (2007).
Shinozaki K., Yamaguchi-Shinozaki K.: “Gene networks
involved in drought stress response and tolerance.” J.
Exp Bot. 58, 221-227 (2007).*
Seki M., Umezawa T., Urano K., Shinozaki K.:
“Regulatory metabolic networks in drought stress
responses.” Curr.Opin. Plant Biol. 10, 296-302 (2007).*
Tran L-S. P., Nakashima K., Shinozaki K., Yamaguchi-
Shinozaki K.: “Plant gene networks in osmotic stress
response: from genes to regulatory networks.” Methods
in Enzymology, 428, 109-128 (2007).
Hirayama T., Shinozaki K.: “Perception and transduction
of abscisic acid signals: keys to the function of the
versatile plant hormone ABA.” Trends in Plant Sci. 12,
343-351 (2007).*
Shinozaki K.: “Acceleration of soybean genomics using
large collection of DNA markers for gene discovery.”
DNA Res., 14, 235 (2008).
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
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Oral Presentations
Umezawa T., Yoshida R., Maruyama K., Yamaguchi-
Shinozaki K. and Shinozaki K.: SRK2C, a SnRK2
protein kinase that improves drought tolerance by
controlling stress-responsive gene expression in
Arabidopsis thaliana, Keystone Symposia on Plant Cell
Signaling: in vivo and omics Approaches, Feb. 1-6, Santa
Fe (2005).
Nishimura T., Kitahata N., Ishida J., Satou M., Seki M.,
Asami T., Shinozaki K. and Hirayama T.: Molecular
genetic analysis of novel ABA hypersensitive mutant,
ahg2, Keystone Symposia on Plant Cell Signaling: in
vivo and omics Approaches, Feb. 1-6, Santa Fe (2005).
Ecker J.R., Chen H., Sundarase A., Borevitz J., Cheuk
R., Kim C.J., Lim J., Ecker J.J., Mockler T., Shinn P.,
Meyers C., Karnes M., Koesema E., Ansari Y., Choy
N., Yamada K., Dale J.M., Wu S.X., Sakano H., Yu G.,
Chang C.H., Pham P., Hsuan V.W., Quach H.L., Jiang
P.X., Lee J.M., Toriumi M., Chan M.M.H., Tang C.C.,
Onodera C.S., Demg J.M., Goldsmith A.D., Vaysberg M.,
Aallender E.K., Wong C., Yamamura Y., Yuan S., Banh J.,
Banno F., Palm C.J., Southwick A.M., Jones T., Nguyen
M., Karlin-Newmann G., Lam B., Miranda M., Gurjal
M., Hansen N.F., Bowser L., Wu T., Tripp M., Tamse R.,
Seki M., Sakurai T., Satou M., Akiyama K., Carninci P.,
Enju A., Hayashizaki Y., Iida K., Ishida J., Arakawa T.,
Kawai J., kamiya A., Nakajima M., Marusaka M., Chan
S., Zhang X., Shinozaki K., Jacobsen S., Davis R.W. and
Theologis A.: Genome-wide discovery of transcription
units and functional elements in Arabidopsis, Keystone
Symposia on Plant Cell Signaling: in vivo and omics
Approaches, Feb. 1-6, Santa Fe (2005).
Ito T., Ohme-Takagi M. and Shinozaki K.: The MS1 gene
of Arabidopsis regulates pollen wall development, 16th
International Conference on Arabidopsis Research, Jun.
15-19, Wisconsin USA (2005)
Motohashi R., Satou M., Myouga F., Ohta D., Okikawa
A. and Shinozaki K.: Metabolomic analysis for
understanding of disrupted nuclear-encoded chloroplast
1.
2.
3.
4.
5.
【International Conferences】protein genes by FT-ICRMS in Arabidopsis thaliana,
16th International Conference on Arabidopsis Research,
Jun. 15-19, Wisconsin USA (2005)
Myouga F., Motohashi R., Iizumi H., Akiyama K. and
Shinozaki K.: Functional genomics of nuclear-encoded
chloroplast proteins in Arabidopsis, 16th International
Conference on Arabidopsis Research, Jun. 15-19,
Wisconsin USA (2005)
Seki M., Ishida J., Iida K., Nakajima M., Enju A., Sakurai
T., Kamei A., Oono Y., Umezawa T., Fujita M., Mizukado
S., Morosawa T., Akiyama K., Narusaka Y., Narusaka
M., Go M., Kobayashi M., Kawai J., Hayashizaki Y. and
Shinozaki K.: Expression profiling using Arabidopsis
whole-genome regulatory gene oligo DNA microarray
and production of Arabidopsis DNAbook containing
about 1000 RAFL cDNA for transcription factors, 16th
International Conference on Arabidopsis Research, Jun.
15-19, Wisconsin USA (2005)
Matsui A., Yokoyama R., Seki M., Shinozaki K.,
Takahashi T., Komeda Y. and Nishitani K.: Functional
diversity and redundancy among members of the XTH
gene family: A specific role of AtXTH27 in vascular
development in rosette leaves of Arabidopsis, 16th
International Conference on Arabidopsis Research, Jun.
15-19, Wisconsin USA (2005)
Kim J.M., Kuromori T., To T., Hirayama T., Seki M.
and Shinozaki K.: Functional analysis of AtORC1a,
Arabidopsis thaliana origin recognition complex, 16th
International Conference on Arabidopsis Research, Jun.
15-19, Wisconsin USA (2005)
Kikuchi J. and Hirayama T.: Novel methods for uniform
stable isotope labeling in plant and animal systems
followed with a hetero-nuclear NMR-based metabomics,
Metabolomics 2005, Jun. 20-23, Tsuruoka Japan (2005)
Chikayama E., Saito K., Shinozaki K. and Kikuchi J.: A
novel method for metabolic profiling of hetero-nuclear
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 181 ―
NMR spectra based on pattern recognition algorithm,
Metabolomics 2005, Jun. 20-23, Tsuruoka Japan (2005)
Sekiyama Y., Saito K., Shinozaki K. and Kikuchi J.:
Preliminary investigation of methodological advances
in a hetero-nuclear NMR-based metabolomics,
Metabolomics 2005, Jun. 20-23, Tsuruoka Japan (2005)
Nakashima K., Tran L-S Phan., Fujita M., Maruyama
K., Todaka D., Ito Y., Shinozaki K. and Yamaguchi-
Shinozaki K.: Expression and functional analysis of
stress-inducible NAC transcription factors in rice plants,
Plant Biology 2005, Jul. 16-20, Seattle USA (2005)
Shinozaki K.: Plant gene networks in osmotic stress
response, 2005 Gordon Research Conference on Cellular
Osmoregulation: Sensors, Transducers and Regulators,
Aug 7-12, Newport USA (2005)
Yamaguchi-Shinozaki K. and Shinozaki K.: Regulatory
network of gene expression in abiotic stress response and
tolerance, 10th International Congress of SABRAO, Aug.
22-23, Tsukuba Japan (2005)
Nakashima K., Tran L-S Phan., Fujita M., Maruyama
K., Todaka D., Ito Y., Shinozaki K. and Yamaguchi-
Shinozaki K.: Molecular characterization of abiotic
stress-inducible OsNAC6, a member of the rice NAC
domain superfamily, 10th International Congress of
SABRAO, Aug. 22-23, Tsukuba Japan (2005)
Fujita Y., Fujita M., Satoh R., Maruyama K., Parvez
M.M., Seki M., Hiratsu K., Ohme-Takagi M., Shinozaki
K. and Yamaguchi-Shinozaki K.: AREB1, an ABA-
induced transcription factor, plays a key role in drought
stress response in Arabidopsis thaliana, 10th International
Congress of SABRAO, Aug. 22-23, Tsukuba Japan
(2005)
Ito T., Ito Y., Maruyama K., Shinozaki K, and Yamaguchi-
Shinozaki K.: Functional analysis of rice transcription
factor OsDREB1F involved in environmental stress-
inducible gene expression, 10th International Congress
of SABRAO, Aug. 22-23, Tsukuba Japan (2005)
Shinozaki K., Yamaguchi-Shinozaki K., Seki M.,
Osakabe Y., Yoshida R., Maruyama K. and Umezawa
T.: Gene networks involved in drought and cold stress
responses, Plant GEMs Amsterdam 2005 on Plant
Genomics European Meetings, Sep 20-23, Amsterdam
The Netherlands (2005)
Ichikawa T., Nakazawa M., Kawashima M., Iizumi H.,
Kuroda H., Kondou Y., Tsuhara Y., Suzuki K., Ishikawa
A., Seki M., Fujita M., Motohashi R., Nagata N., Takagi
T., Shinozaki K. and Matsui M.: Characterization of two
dominant mutants caused by ectopic expression of full-
length cDNA in Arabidopsis FOX hunting system, Plant
GEMs Amsterdam 2005 on Plant Genomics European
Meetings, Sep 20-23, Amsterdam The Netherlands (2005)
Shinozaki K. and Yamaguchi-Shinozaki K.: Gene
networks involved in drought stress responses and
tolerance, The 2nd International Conference on Integrated
Approaches to Sustain and Improve Plant Production
Under Drought Stress, Sep. 24-28, Rome Italy (2005)
Seki M., Ishida J., Nakajima M., Enju A., Sakurai T., Iida
K., Satou M., Akiyama K., Oono Y., Fujita M., Mizukado
S., Kamei A., Narusaka M., Narusaka Y., Morosawa T.,
Kim J.M., Yamaguchi-Shinozaki K., Go M., Toyoda T.
and Shinozaki K.: Arabidopsis encyclopedia using full-
length cDNAs and its application for expression profiling
under abiotic stress conditions, The 2nd International
Conference on Integrated Approaches to Sustain and
Improve Plant Production Under Drought Stress, Sep.
24-28, Rome Italy (2005)
Urano K., Maruyama K., Takeda M., Suzuki H., Saito
K., Shibata D., Yamaguchi-Shinozaki K. and Shinozaki
K.: Metabolome and transcriptome analysis of ABA
responsive pathway under drought stress in Arabidopsis
thaliana, The 2nd International Conference on Integrated
Approaches to Sustain and Improve Plant Production
Under Drought Stress, Sep. 24-28, Rome Italy (2005)
Ito Y., Katsura K., Taji T., Kobayashi M., Seki
M., Shinozaki K. and Yamaguchi-Shinozaki K.:
12.
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 182 ―
Overexpression of the DREB1/CBF gene family
improved stress tolerance to drought, high salt and low
temperature in rice, The 2nd International Conference
on Integrated Approaches to Sustain and Improve Plant
Production Under Drought Stress, Sep. 24-28, Rome
Italy (2005)
Umezawa T.: Arabidopsis SNRK2 protein kinases and
osmotic stress signaling, Xth France-Japan Workshop on
Plant Sciences, Sep. 25-29, Toulouse France (2005)
Yamaguchi-Shinozaki K., Osakabe Y., Mizuno S.,
Maruyama K., Seki M. and Shinozaki K.: A leucine-
rich repeat receptor like kinase involved in perception of
ABA signal, Protein Phosphorylation in plant Signaling
2005, Oct. 20-21, Tsukuba Japan (2005)
Yoshida R., Umezawa T., Ichimura K., Mizoguchi T.,
Takahashi F., Takahashi S. and Shinozaki K.: Arabidopsis
SnRK2 plays important roles in both ABA and osmotic
stress signaling, Protein Phosphorylation in plant
Signaling 2005, Oct. 20-21, Tsukuba Japan (2005)
Seki M., Ishida J., Nakajima M., Enju A., Sawasaki
T., Endo Y., Oono Y., Morosawa T., Kim J.M., Sakurai
T., Iida K., Kamei A., Narusaka M., Narusaka Y. and
Shinozaki K.: Arabidopsis functional genomics of protein
kinases and transcription factors, Protein Phosphorylation
in plant Signaling 2005, Oct. 20-21, Tsukuba Japan
(2005)
Mizuno S., Osakabe Y., Maruyama K., Shinozaki K.
and Yamaguchi-Shinozaki K.: Regulation of floral
development by the LRR receptor protein kinase
2(RPK) in Arabidopsis, Protein Phosphorylation in plant
Signaling 2005, Oct. 20-21, Tsukuba Japan (2005)
Fujita Y., Fujita M., Satoh R., Maruyama K., Parvez
M.M., Seki M., Hiratsu K., Ohme-Takagi M., Shinozaki
K. and Yamaguchi-Shinozaki K.: AREB1 regulates novel
ABRE-dependent ABA-signaling that enhances drought
stress tolerance during the vegetative growth phase in
Arabidopsis thaliana, Protein Phosphorylation in plant
Signaling 2005, Oct. 20-21, Tsukuba Japan (2005)
Takahashi F., Yoshida R., Ichimura K., Mizoguchi T.,
Yonezawa M., Maruyama K., Yamaguchi-Shinozaki K.
and Shinozaki K.: A novel MAP kinase cascade, MKK3-
MPK6, regulates jasmonate signaling in Arabidopsis,
Protein Phosphorylation in plant Signaling 2005, Oct.
20-21, Tsukuba Japan (2005)
Osakabe Y., Maruyama K., Seki M., Shinozaki K.
and Yamaguchi-Shinozaki K.: An LRR receptor
kinase, RPK1, is a key membrane-bound regulator of
abscisic acid early signaling in Arabidopsis, Protein
Phosphorylation in plant Signaling 2005, Oct. 20-21,
Tsukuba Japan (2005)
Yoshida T., Nishimura N., Kitahata N., Kuromori
T., Ito T., Asami T., Shinozaki K. and Hirayama T.:
ABA Hypersensitive Germination3 encodes a protein
phosphatase 2C(AtPP2CA) that strongly ABA signaling
during germination among Arabidopsis PP2Cs, Protein
Phosphorylation in plant Signaling 2005, Oct. 20-21,
Tsukuba Japan (2005)
Umezawa T., Yoshida R., Maruyama K., Yamaguchi-
Shinozaki K. and Shinozaki K.: An osmotic-stress
activated protein kinase SRK2C mediates stress signaling
in Arabidopsis, Protein Phosphorylation in plant
Signaling 2005, Oct. 20-21, Tsukuba Japan (2005)
Katagiri T., Kobayashi M. and Shinozaki K.: An
important role of phosphatidic acid in ABA signaling
during germination in Arabidopsis thaliana, International
Conference on Plant Lipid-Mediated Signaling: Building
Connections, Oct. 26-29, Raleigh USA (2005)
Yamaguchi-Shinozaki K. and Shinozaki K.: Functional
analysis of plant transcription factors involved in abiotic
stress response, CREST-JST International Symposium
2005 on Functional Network of Transcription Factors in
Plants, Nov. 16-17, Tsukuba Japan (2005)
Mitsuda N., Seki M., Shinozaki K. and Ohme-Takagi
M.: Transcription factors regulating secondary wall
thickenings, CREST-JST International Symposium 2005
25.
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 183 ―
on Functional Network of Transcription Factors in Plants,
Nov. 16-17, Tsukuba Japan (2005)
Seki M., Ishida J., Nakajima M., Enju A., Morosawa
T., Kim J.M., Sakurai T., Iida K., Oono Y., Kamei A.,
Narusaka M., Narusaka Y., Toyoda T., Mochizuki Y.,
Hasegawa Y., Yamaguchi-Shinozaki K. and Shinozaki
K.: Arabidopsis whole-genome expression profiling
under abiotic stress conditions, CREST-JST International
Symposium 2005 on Functional Network of Transcription
Factors in Plants, Nov. 16-17, Tsukuba Japan (2005)
Fujita M., Fujita Y., Maruyama K., Seki M., Ohme-
Takagi M., Yamaguchi-Shinozaki K. and Shinozaki K.: A
dehydration-induced NAC protein, RD26 is involved in a
novel ABA-dependent stress-signaling pathway, CREST-
JST International Symposium 2005 on Functional
Network of Transcription Factors in Plants, Nov. 16-17,
Tsukuba Japan (2005)
Shinozaki K. and Yamaguchi-Shinozaki K.: Functional
genomics for gene discovery in abiotic stress response
and tolerance, 5th International Rice Genetics
Symposium and 3rd International Rice Functional
Genomics Symposium, Nov. 19-23, Manila Philippines
(2005)
Yamaguchi-Shinozaki K. and Shinozaki K.: Rice
promoters for the generation of stress tolerant transgenic
crops, 5th International Rice Genetics Symposium and
3rd International Rice Functional Genomics Symposium,
Nov. 19-23, Manila Philippines (2005)
Todaka D., Ito Y., Ohme-Takagi M., Shinozaki K.
and Yamaguchi-Shinozaki K.: Functional analysis of
OsPIF1 gene down-regulated by drought stress in rice,
5th International Rice Genetics Symposium and 3rd
International Rice Functional Genomics Symposium,
Nov. 19-23, Manila Philippines (2005)
38.
39.
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42.
Shinzoaki K. and Yamaguchi-Shinozaki K.: Molecular
responses and tolerance to drought stress in higher plants,
The First International Conference on the Theory and
Plactices in Biological Water Saving, May 21-25, Beijing
43.
China (2006).
Shinozaki K. and Yamaguchi-Shinozaki K.: Gene
networks involved in drought stress responses and
tolerance, Bio Vision Alexandira 2006, Apri.26-29,
Alexandria Egypt (2006).
Shinozaki K. and Yamaguchi-Shinzoaki K.: Signaling
and transcription networks in drought stress response,
Keystone Symposia on Plant Responses to Abiotic Stress,
April 8-13, Colorado USA (2006).
Seki M., Ishida J., Morosawa T., Matusi A., Kim
J.M., Nakajima M., Enju A., To T., Iida K., Go M.,
Mochizuki Y., Hasegawa Y., Toyoda T. and Shinzoaki
K.: “Arabidopsis whole-genome transcriptome analysis
under abiotic stress conditions”, The 16th Penn State
Symposium in Plant Physiologyon RNA Biology: Novel
Insights from Plant Systems, May 18-20, Penn State
USA (2006).
Ito T., Nagata N., Ohme-Takagi M., Ma H. and
Shinozaki K.: An Arabidopsis MALE STERILITY1 gene
encoding a PHD-type transcription factor regulates early
microgametogenesis, The 16th Penn State Symposium in
Plant Physiology on RNA Biology: Novel Insights from
Plant Systems, May 18-20, Penn State USA (2006).
Hirayama T., Tian C., Tsuboi Y., Chikayama E.,
Sekiyama Y., Kuromori T., Shinozaki K. and Kikuchi J.:
Advances in hetero-nuclear NMR-based metabolomics
approach in plant systems, 20th IUBMB Internationa
Congress of Biochemistry and Molecular Biology and
11th FAOBMB Congress, June 18-23, Kyoto Japan
(2006).
Tsuboi Y., Hirayama T., Shinozaki K. and Kikuchi J.:
Toward an NMR-based nutrigenomics assisted by stable
isotope labeling of plants through animals, 20th IUBMB
Internationa Congress of Biochemistry and Molecular
Biology and 11th FAOBMB Congress, June 18-23,
Kyoto Japan (2006).
Hirayama T., Tsuboi Y., Shinozaki K. and Kikuchi
44.
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48.
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50.
Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 184 ―
J.: Toward an NMR-based nutrigenomics assisted
by stable isotope labeling of plants through animals,
Metabolomics 2006 on the Second Scientific Meeting
of the Metabolomics Society, June 24-29, Boston USA
(2006).
Tian C., Tsuboi Y., Chikayama E., Sekiyama Y.,
Kuromori T., Shinozaki K., Hirayama T. and Kikuchi J.:
An efficient strategy for the metabolic characterization of
Arabidopsis mutants with visible and silent phenotypes
by fast 1D-NMRscreening followed multi-dimensional
NMR analysis, Metabolomics 2006 on the Second
Scientific Meeting of the Metabolomics Society, June
24-29, Boston USA (2006).
Ichikawa T., Nakazawa M., Kondou Y., Ishikawa A.,
Kawashima M., Iizumi H., Seki M., Fujita M., Muto S.,
Shinozaki K. and Matsui M.: Evaluation of Arabidopsis
FOX line: T2 phenotype and phenotype recapitulation
by transformation, 17th International Conference on
Arabidopsis Research, June 28- July 2, Wisconsin-
Madison USA (2006).
Ichimura K., Casais C., Peck S., Shinozaki K. and
Shirasu K.: MEKK1 negatively regulates tissue specific
and temperature dependent cell death in Arabidopsis,
17th International Conference on Arabidopsis Research,
June 28- July 2, Wisconsin-Madison USA (2006).
Katagiri T. and Shinozaki K.: Functional analysis of
phosphatidic acid during germination, 17th International
Conference on Arabidopsis Research, June 28- July 2,
Wisconsin-Madison USA (2006).
Motohashi R., Okada E., Kuroda H., Nagata N., Matsui
M. and Shinozaki K.: Functional analysis of ribosome
binding factor (RBFA) homologue using Ac/Ds
transposon system in Arabidopsis thaliana. Plant Biology
2006, Aug. 5-9, Boston USA (2006).
Nishimura N., Yoshida T., Kitahata N., Asami T.,
Shinozaki K. and Hirayama T.: Characterization of novel
ABA hypersensitive mutant, ahg1,Plant Biology 2006,
Aug. 5-9, Boston USA (2006).
Hirayama T., Tian C., Tsuboi Y., Chikayama E.,
Sekiyama Y., Kuromori T., Shinozaki K. and Kikuchi J.:
Adcances in hetero-nuclear NMR-based metabolomics
approach in plant systems, Plant Biology 2006, Aug. 5-9,
Boston USA (2006).
Hayashi S., Kuromoti T., Shinozaki K. and Hirayama T.:
Analysis of an Arabidopsis root hair defective mutant
shv3, Plant Biology 2006, Aug. 5-9, Boston USA (2006).
Yoshida T., Nishimura N., Kitahata N., Kuromori
T.,Kuroda H., Matsui M., Asami T., Shinozaki K. and
Hirayama T.: ABA-hypersensitive germination3 encodes
a protein phosphatase 2C (AtPP2CA) that strongly
regulates abscisic acid signaling during germination
among Arabidopsis protein phosphatase 2Cs, Plant
Biology 2006, Aug. 5-9, Boston USA (2006).
Shinozaki K. and Yamaguchi-Shinozaki K.: Regulatory
gene networks in drought stress responses and tolerance,
Tropical Crop Biotechnology Conference 2006, Aug.
16-19, Cairns Queensland Australia (2006).
Shinzoaki K. and Yamaguchi-Shinozaki K.: Regulatory
networks in drought stress response and tolerance, 8th
International Congress of Plant Molecular Biology, Aug.
20-25, Adelaide Australia (2006).
Seki M., Ishida J., Morosawa T., Matsui A., Kim J.M.,
Nakajima M., Enju A., To T., Iida K., Go M., Mochizuki
Y., Hasegawa Y., Kaminuma E., Toyoda T. and Shinozaki
K.: Arabidopsis whole-genome expression profiling under
abiotic stress conditions, 8th International Congress of
Plant Molecular Biology, Aug. 20-25, Adelaide Australia
(2006).
Myouga F., Akiyama K., Sakurai T., Motohashi R. and
Shinozaki K.: Gene discovery for chloroplast proteins
involved in drought tolerance in Arabidopsis using
the rarge database, 8th International Congress of Plant
Molecular Biology, Aug. 20-25, Adelaide Australia
(2006).
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 185 ―
Yasuda M., Ishikawa A., Asami T., Kamiya Y., Shinozaki
K., Kudo T., Yoshida S. and Nakashita H.: Antagonistic
interaction between environmental stress response
and systemic acquired resistance in Arabidopsis, 8th
International Congress of Plant Molecular Biology, Aug.
20-25, Adelaide Australia (2006).
Kim J.M., Kuromori T., To T., Hirayama T., Seki M.
and Shinozaki K.: Functional analysis of AtORC1a in a
phase progression, 8th International Congress of Plant
Molecular Biology, Aug. 20-25, Adelaide Australia
(2006).
Nakamura H., Hakata M., Kajikawa M., Ando S.,
Amano K., Toki N., Higashi N., Fujita M., Enju A., Seki
M., Nakazawa M., Ichikawa T., Matsui M., Shinzoaki
K., Miyao A., Toki T., Hirochika H. and Ichikawa H.:
Genome-wide functional analysis of rice genes using
FOX hunting system 1. Overview and current status, 8th
International Congress of Plant Molecular Biology, Aug.
20-25, Adelaide Australia (2006).
Hakata M., Nakamura H., Asano T., Kajikawa M.,
Amano K., Toki N., Higashi N., Fujita M., Enju A.,
Seki M., Nakazawa M., Ichikawa T., Shinozaki K.,
Matsui M., Komatsu S., Miyao A., Toki S., Hirochika
H. and Ichikawa H.: Genome-wide functional analysis
of rice genes using FOX hunting system 2. Analysis and
identificaiton of introduced cDNAs in the FOX-rice lines,
8th International Congress of Plant Molecular Biology,
Aug. 20-25, Adelaide Australia (2006).
Ichikawa H., Nakamura H., Hakata M., Kajikawa M.,
Ando S., Amano S., Toki N., Higashi N., Fujita M.,
Enju A., Seki M., Nakazawa M., Ichikawa T., Matsui
M., Shinozaki K., Miyao A., Toki T. and Hirochika H.:
FOX hunting system: a genome-wide functional analysis
of rice genes using transgenic plants, 8th International
Congress of Plant Molecular Biology WorkShop, Aug.
20-25, Adelaide Australia (2006).
Urano K., Maruyama K., Takeda M., Suzuki H., Saito
K., Shibata D., Yamaguchi-Shinozaki K. and Shinozaki
K.: Analysis of ABA-dependent metabolic networks
in drought stress response, 2006 Gordon Research
Conference on Salt & Water Stress in Plants, Sep. 3-8,
Oxford UK (2006).
Tran L-S., Urao T., Qin F., Osakabe Y., Kakimoto T.,
Shinozaki K. and Yamaguchi-Shinozaki K.: Functional
analyses of AtHK1, AHK2, AHK3 and AHK4/CRE1
in response to abiotic stresses, 2006 Gordon Research
Conference on Salt & Water Stress in Plants, Sep. 3-8,
Oxford UK (2006).
Fujita Y., Fujita M., Satoh R., Maruyama K., Sayama
H., Seki M., Hiratsu K., Ohme-Takagi M., Shinzoaki K.
and Yamaguchi-Shinozaki K.: AREB1 mediates novel
ABRE-dependent ABA-signaling that enhances drought
stress tolerance in Arabidopsis thaliana, 2006 Gordon
Research Conference on Salt & Water Stress in Plants,
Sep. 3-8, Oxford UK (2006).
Shinozaki K.: Functional genomics in RIKEN Plant
Science Center for the improvement of plant productivity,
PKU-CCAST-RIKEN Joint Symposium-Life Science
Panel, Nov. 10, Beijing China (2006).
Yamaguchi-Shinozaki K. and Shinozaki K.: Regulatory
networks of gene expression in environmental stress
response, GARNet 2006, Sep 11-12, Bristol UK (2006).
Nakashima K., Tran L-S Phan., Dong NV., Fujita M.,
Maruyama K., Todaka D., Ito Y., Shinozaki K. and
Yamaguchi-Shinozaki K.: Functional analysis of NAC-
type transcription factor OsNAC6 involved in abiotic
stress-responsive gene expression in rice, 4th Internationa
Rice Functional Genomics Symposium, Oct. 9-11,
Montpellier France (2006).
Umezawa T., Todaka D., Totoki Y., Sakurai T., Seki M.,
Toyoda A., Maruyama K., Nakashima K., Mizukado S.,
Kasuga M., Ahmed S, Sado H., Harada K., Tsubokura Y.,
Sato S., Anai T., Ishimoto M., Funatsuki H., Teraishi M.,
Shinano T., Akashi R., Sakaki Y., Yamaguchi-Shinozaki
K. and Shinozaki K.: Construction of a soybean full-
length cDNA library and oligonucleotide microarray, The
KAZUSA Conference on legume Genetics and Genomics
64.
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 186 ―
in Asia, Nov. 27-28, Kisarazu Japan (2006).
Todaka D., Nakashima K., Ito Y., Ohme-Takagi M.,
Shinozaki K. and Yamaguchi-Shinozaki K.: Functional
analysis of the OsPIF1 gene down-regulated by drought
stress in rice. ICGEB Meeting: Biotic and Abiotic Stress
Responses in Plants, Dec 11-13, New Delhi, India. (2006)
.
Qin F., Sakuma Y., Tran L-S P., Osakabe Y., Shinozaki
K. and Yamaguchi-Shinzoaki K.: The function and
regulation of an Arabidopsis transcription factor
DREB2A in plant water deficit and high temperature
stresses, Gordon Research Conferences on Temperature
Stress in Plants, Jan 21-26, Ventura CA (2007).
Osakabe Y., Mizuno S, Shinozaki K. and Yamaguchi-
Shinozaki K.: Functional analysis of a leucine-rich
repeat receptor like kinase, RPK1, involved in ABA
signal transduction of Arabidopsis, Gordon Research
Conferences on Temperature Stress in Plants, Jan 21-26,
Ventura CA (2007).
Ito Y., Shinozaki K. and Yamaguchi-Shinozaki K.:
Comprehensive analysis of 10 DREB/CBF family genes
in rice, Gordon Research Conferences on Temperature
Stress in Plants, Jan 21-26, Ventura CA (2007).
Kidokoro S., Maruyama K., Nakashima K., Sakuma
Y., Imura Y., Shinozaki K. and Yamaguchi-Shinozaki
K.: Transcriptional regulation of the DREB1C gene
in response to low temperature, Gordon Research
Conferences on Temperature Stress in Plants, Jan 21-26,
Ventura CA (2007).
Seki M., Matsui A., Kim J-M., Ishida J., Nakajima
M., Morosawa T., Kawashima M., Satou M., Kim T.,
Kurihara Y., Kaminuma E., Endo T., Mochizuki Y.,
Kobayashi N., Toyoda T. and Shinozaki K.: Arabidopsis
whole-genome transcriptome analysis under drought,
cold, high-salinity, and ABA treatment conditions
using tiling array and 454 sequencing technology, 24th
Symposium in Plant Biology on Gene Silincing: The
Biology of Small RNAs and the Epigenome, Jan. 18-20,
Riverside USA (2007).
Shinozaki K.: Functional genomics in drought stress
responses, CINA-JAPAN Joint Workshop on Plant
Molecular Breeding, Mar 12-13, Beijing China (2007).
Shinozaki K.: Molecular biology and salinity stress
responses and tolerance, BIOTEC-RIKEN Research
Forum, May 22-25, Thailand (2007).
Shinozaki K., Yamaguchi-Shinozaki K. and Seki M.:
Regulatory gene network in drought and ABA responses.,
18th International Conference on Arabidopsis Research.,
June 20-23., Beijing China (2007).
Mitsuda N., Iwase A., Yamamoto H., Yoshida M.,
Seki M., Shinozaki K. and Ohme-Takagi M.: NAC
transcription factors NST1, NST2 and NST3 are key
regulators of secondary wall formation in Arabidopsis,
18th Internat ional Conference on Arabidopsis
Research.,June 20-23., Beijing China (2007).
Ichikawa T., Nakazawa M., Kawashima M., Iizumi H.,
Kuroda H., Kondou Y., Ishikawa A., Seki M., Fujita
M., Higuchi M., Shinozaki K. and Matsui M.: The Fox
hunting system: an alternative gain-of- function gene
hunting technique, 18th International Conference on
Arabidopsis Research.,June 20-23., Beijing China (2007).
Seki M., Matsui A., Kim J-M., Ishida J., Nakajima
M., Morosawa T., Kawashima M., Satou M., Kim T.,
Kurihara Y., Kaminuma E., Endo T., Mochizuki Y.,
Kobayashi N., Toyoda T. and Shinozaki K.: Arabidopsis
whole-genome transcriptome analysis under drought,
conditions using tiling array and 454 sequencing
technology, 18th International Conference on Arabidopsis
Research.,June 20-23., Beijing China (2007).
Kuromori T., Kamiya A., Ito T., Wada T., Hirayama T.
and Shinozaki K.: A mutant-based phenome analysis
and collection of mutant phenotype information, 18th
International Conference on Arabidopsis Research.,June
20-23., Beijing China (2007).
Myouga F., Iizumi H., Kuromori T., Motohashi R. and
76.
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 187 ―
Shinozaki K.: Characterization of Ds-tagged mutants for
nuclear-encoded chloroplast protein involved in plastid
development and oxi-dative stress responses, 18th
International Conference on Arabidopsis Research.,June
20-23., Beijing China (2007).
Hirayama T., Narusaka Y., Yasuda M., Narusaka M.,
Hayashi S., Kitahata N., Kuromori T., Asami T. and
Shinozaki K.: Impairment in the mRNA degradation
causes abnormal response to both abscisic acid and
salicylic acid in Arabidopsis, 18th International
Conference on Arabidopsis Research.,June 20-23.,
Beijing China (2007).
Urano K., Maruyama K., Takeda M., Sakurai N., Suzuki
H., Saito K., Shibata D., Yamaguchi-Shinozaki K. and
Shinozaki K.: Comprehensive analysis of ABA-regulated
metabolome and transcriptome in drought response
of Arabidopsis, 18th International Conference on
Arabidopsis Research.,June 20-23., Beijing China (2007).
Takahashi F., Yoshida R., Ichimura K., Mizoguchi T.,
Yonezawa M., Maruyama K., Yamaguchi-Shinozaki K.
and Shinozaki K.: The MAP kinase cascade MKK3-
MPK6 is an important part of the jasmonate signal
transduction pathway in Arabidopsis, 18th International
Conference on Arabidopsis Research.,June 20-23.,
Beijing China (2007).
Garcia M., Myouga F., Takechi K., Sato H., Nabeshima
K., Nagata N., Takio S., Shinozaki K. and Takano H.:
An Arabidopsis chloroplast-targeting MurE homolog
has an essential role in chloroplast development, 18th
International Conference on Arabidopsis Research.,June
20-23., Beijing China (2007).
Shinozaki K. and Yamaguchi-Shinozaki K.: Regulatory
gene networks in drought and ABA responses, IPGSA
19th Annual Meeting, July21-25, Puerto Vallarta Mexico
(2007).
Ayano M., Godal H., Shinozaki K., Watanabe N.,
Yokoyama M. and Yoshida S.: An oxylipin, (12Z, 15Z)-
9-hydroxy-10-oxo octadecadienoic acid [KODA] shows
unique signaling on floral development of higher plants,
IPGSA 19th Annual Meeting, July21-25, Puerto Vallarta
Mexico (2007).
Ayano M., Godal H., Shinozaki K., Watanabe N.,
Yokoyama M. and Yoshida S.: Function analysis of
oxylipin, 9-hydroxy-10-oxo12(z), 15(z)-octadecadienoic
acid (KODA) in Arabidopsis thaliana, IPGSA 19th
Annual Meeting, July21-25, Puerto Vallarta Mexico
(2007).
Nishimura N., Yoshida T., Kitahata N., Asami T.,
Shinozaki K. and Hirayama T.: ABA-hypersensitive
germination encodes a protein phosphatase 2C, an
essential component of abscisic acid signaling in
Arabidopsis seed, IPGSA 19th Annual Meeting,
July21-25, Puerto Vallarta Mexico (2007).
Goda H., Yoshida S., Shinozaki K. and Shimada Y.:
Finding of a novel hormone-related compound by using
AtGenExpress microarray data, IPGSA 19th Annual
Meeting, July21-25, Puerto Vallarta Mexico (2007).
Shinozaki K. and Yamaguchi-Shinozaki K.: Regulatory
gene network in drought stress response, 2nd World
Conference of Stress, August 23-26, Butapest Hungary
(2007).
Yamaguchi-Shinozaki K. and Shinozaki K.: Improving
abiotic stress-tolerance in crops,Frontiers in Transgenesis,
Sep 26-28, St Louis USA (2007).
Yamaguchi-Shinozaki K. and Shinozaki K.: Regulatory
networks of gene expression in abiotic stress responses in
Arabidopsis and rice, 2007 International Symposium and
Annual Meeting of the KSABC, Oct 11-13, Gyeongiu
Korea, (2007).
Matsukura S., Yoshida T., Todaka D., Ito Y., Maruyama
K., Shinozaki K. and Yamaguchi-Shinozaki K.:
Functional analysis of the OsDREB2B gene encoding
a rice transcription factor involved in abiotic-stress-
responsive gene expression, 2007 International
Symposium and Annual Meeting of the KSABC, Oct
90.
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 188 ―
11-13, Gyeongiu Korea, (2007).
Nakashima K., Tran L-S Phan., Dong NV., Fujita M.,
Maruyama K., Todaka D., Ito Y., Hayashi N., Shinozaki
K. and Yamaguchi-Shinozaki K.: Functional analysis
of NAC-type transcription factor OsNAC6 involved in
abiotic stress-responsive gene expression in rice, 2007
International Symposium and Annual Meeting of the
KSABC, Oct 11-13, Gyeongiu Korea, (2007).
Yamada K., Osakabe Y., Maruyama K., Fujita Y., Mizoi J.,
Shinozaki K. and Yamaguchi-Shinozaki K.: Functional
analysis of osmotic stress-inducible sugar transporter
gene in Arabidopsis. 2007 International Symposium and
Annual Meeting of the KSABC, Oct 11-13, Gyeongiu
Korea, (2007).
Ito Y., Maruyama K., Shinozaki K. and Yamaguchi-
Shinozaki K.: Comprehensive analysis of DREB1/CBF
family genes in rice, 2007 International Symposium and
Annual Meeting of the KSABC, Oct 11-13, Gyeongiu
Korea, (2007).
Yamaguchi-Shinozaki K. and Shinozaki K.: Improving
transgenic tolerance to abiotic stresses including
drought in rice, The 5th International Symposium of rice
functional genomics,Oct. 15-17, Tsukuba Japan (2007).
Todaka D., Nakashima K., Ito Y., Ohme-Takagi M.,
Shinozaki K. and Yamaguchi-Shinozaki K.: Functional
analysis of the OsPIF1 gene down-regulated by drought
stress in rice.The 5th International Symposium of rice
functional genomics, Oct. 15-17, Tsukuba Japan (2007).
Ito Y., Maruyama K., Shinozaki K. and Yamaguchi-
Shinozaki K.: Comprehensive analysis of DREB1/CBF
family genes in rice, The 5th International Symposium
of rice functional genomics,Oct. 15-17, Tsukuba Japan
(2007).
Matsukura S., Yoshida T., Todaka D., Ito Y., Maruyama
K., Shinozaki K. and Yamaguchi-Shinozaki K.:
Functional analysis of the OsDREB2B gene encoding
a rice transcription factor involved in abiotic-stress-
responsive gene expression,The 5th International
Symposium of rice functional genomics, Oct. 15-17,
Tsukuba Japan (2007).
Nakashima K., Tran L-S Phan., Nguyen D.V., Fujita M.,
Maruyama K., Todaka D., Ito Y., Hayashi N., Shinozaki
K. and Yamaguchi-Shinozaki K.: Functional analysis of
a NAC-type transcription factor OsNAC6 involved in
abiotic and biotic stress-responsive gene expression in
rice, The 5th International Symposium of rice functional
genomics, Oct. 15-17, Tsukuba Japan (2007).
Shinozaki K., Yamaguchi-Shinozaki K. and Seki M.:
Gene network in drought stress response, 2007 Genomics
Symposium on The Genomics of Drought, Oct 22-24,
Adelaide Australia (2007).
Shinozaki K.: Plant functional genomics in drought stress
response, National Tipei University Special Seminar, Oct
15-18, Taipei China (2007).
Seki M.: Arabidopsis whole-genome transcriptome
analysis under drought, cold, high-salinity, and
ABA treatment conditions using tiling array and 454
sequencing technology, 2007 Iwate International
Symposium on Biological Sciences --The Impacts of
OMICS--, Oct. 29, Morioka Japan (2007).
Shinozaki K., Yamaguchi-Shinozaki K. and Seki M.:
Plant functional genomics in drought stress response,
The 6th Asian Crop Science Association Conference and
The 2nd International Conference on Rice for the Future,
Nov. 5-9, Bangkok Thailand (2007).
Yamaguchi-Shinozaki K. and Shinozaki K.: Regulation
of gene expression in response to abiotic stresses and
improving stress tolerance in crops, Agricultural Plant
Stress Response VII on abiotic stress and Plant Growth
and Development, Nov. 8, Gwangju Korea (2007).
Arinaga N., Osakabe Y., Yamada K., Maruyama K.,
Shinozaki K. and Yamaguchi-Shinozaki K.: Functional
analysis of an osmotic stress-inducible potassium
transporter, KUP6, in Arabidopsis, Agricultural Plant
103.
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106.
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109.
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111.
112.
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114.
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Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 189 ―
Stress Response VII on abiotic stress and Plant Growth
and Development, Nov. 8, Gwangju Korea (2007).
Maruyama K., Takeda M., Sakurai N., Kidokoro S.,
Yamada K., Suzuki H., Saito K., Shinozaki K. and
Yamaguchi-Shinozaki K.: A comparative analytic
approach to the chilling/drought metabolites and
transcripts: DREB1A/CBF3 and chloroplastic raffinose
synthase playing a key role in low-temperature condition,
Agricultural Plant Stress Response VII on abiotic stress
and Plant Growth and Development, Nov. 8, Gwangju
Korea (2007).
Kanamori N., Fujita Y., Yoshida T., Sayama H., Fujita
M., Maruyama K., Hiratsu K., Ohme-Takagi M.,
Seki M., Shinozaki K. and Yamaguchi-Shinozaki K.:
Molecular characterization of AREB family transcription
factors involved in ABA-responsive gene expression in
Arabidopsis thaliana, Agricultural Plant Stress Response
VII on abiotic stress and Plant Growth and Development,
Nov. 8, Gwangju Korea (2007).
Osakabe Y., Mizuno S., Maruyama K., Osakabe K.,
Shinozaki K. and Yamaguchi-Shinozaki K.: Functional
analysis of a leucine-rich repeat receptor like kinase,
RPK1, involved in ABA signal transduction of
Arabidopsis, Agricultural Plant Stress Response VII on
abiotic stress and Plant Growth and Development, Nov. 8,
Gwangju Korea (2007).
Todaka D., Nakashima K., Ito Y., Ohme-Takagi M.,
Shinozaki K. and Yamaguchi-Shinozaki K.: Functional
analysis of the OsPIFI gene down-regulated by drought
stress in rice, Agricultural Plant Stress Response VII on
abiotic stress and Plant Growth and Development, Nov. 8,
Gwangju Korea (2007).
Yoshida T., Sakuma Y., Todaka D., Osakabe Y.,
Maruyama K., Shinozaki K. and Yamaguchi-Shinozaki
K.: Functional analysis of Arabidopsis HsfA3 controlled
by DREB2A regulatory system under heat stress,
Agricultural Plant Stress Response VII on abiotic stress
and Plant Growth and Development, Nov. 8, Gwangju
Korea (2007).
Fujita Y., Yamaguchi-Shinozaki K. and Shinozak K.:
Improving drought and salt stress tolerance in plants
by gene transfer, Fourth Biomass-Asia Workshop, Nov
20-22, Selangor Darul Ehsan, Malaysia (2007).
Tanaka R., Nagane R., Myouga F., Motohashi R.,
Shinozaki K. and Tanaka A.: HPLC-based screening for
pigment metabolism mutants in Arabidopsis thaliana, 7th
International Conference on Tetrapyrrole Photoreceptors
in Photosynthetic Organisms, Dec. 9-14, Kyoto Japan
(2007).
Shinozaki K., Urano K., Maruyama K., Takeda M.,
Ogata Y., Sakurai N., Suzuki H., Saito K., Shibata D.,
Kobayashi M. and Yamaguchi-Shinozaki K.: Regulatory
gene network in drought-induced metabolic change,
5th International Conference on Plant Metabolomics,
Yokohama Japan, Jul 15-18 (2008).
Mochida K., Furuta T., Ebana K., Ogihara Y., Shinozaki
K and Kikuchi J: Metabolic phenotyping of genetic
resources in gramineae using NMR, 5th International
Conference on Plant Metabolomics, Yokohama Japan,
Jul 15-18 (2008).
Akiyama K., Chikayama E., Yuasa H., Shimada Y.,
Tohge T., Shinozaki K., Yokota-Hirai M., Sakurai T.,
Kikuchi J. and Saito K.: PRIME: The Platform for
RIKEN metabolomics to assist systems biology studies,
5th International Conference on Plant Metabolomics,
Yokohama Japan, Jul 15-18 (2008).
Sakurai T., Kondou Y., Akiyama K., Sakakibara H.,
Kusano M., Takahashi H., Mori M., Takatsuji H.,
Shinozaki K., Oda K., Hirochika H. and Matsui M.:
Construction of RIKEN rice FOX Arabidopsis mutant
line database, 5th International Conference on Plant
Metabolomics, Yokohama Japan, Jul 15-18 (2008).
Urano K., Maruyama K., Takeda M., Ogata Y., Sakurai
N., Suzuki H., Saito K., Shibata D., Kobayashi
M., Yamaguchi-Shinozaki K. and Shinozaki K.:
Characterization of the ABA-regulated global responses
117.
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125.
126.
127.
128.
Shinozaki Research Collaborative GroupRIKEN BRC Annual Report 2005 ~ 2007
― 190 ―
to dehydration in Arabidopsis by metabolomics, 5th
International Conference on Plant Metabolomics,
Yokohama Japan, Jul 15-18 (2008).
Maruyama K., Kidokoro S., Takasaki H., Sakurai N.,
Suzuki H., Saito K., Shibata D., Shinozaki K. and
Yamaguchi-Shinozaki K.: A comparative analysis of
transcripts and metabolites in rice under chilling and
dehydration conditions, 5th International Conference on
Plant Metabolomics, Yokohama Japan, Jul 15-18 (2008).
129.
【Domestic Conferences】 Total 210
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