biomarkers in terrestrial ecotoxicology: stress ecology in bees
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Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees
Chair: Dr. Fábio Camargo Abdalla, UFSCar – Sorocaba, fabdalla@ufscar.br
“State of art of morphological biomarkers in ecotoxicology researche on bees”
Speaker 1: Caio Eduardo da C. Domingues - Post-graduation in Biotechnology & Environmental Monitoring
“Fat body, pericardial cells and hemocytes in association with the myogenic region: a model system to analysis the stress ecology in biomarkers of bees”
Speaker 2: Paulo José Balsamo – Post-graduation in Biotechnology & Environmental Monitoring
“An old subject, but a new challenge in ecotoxicology applied to the study of morphological biomarkers in bees: Hormesis”
Speaker 3: Dr. Elaine Cristina Mathias da Silva-Zacarin, UFSCar – Sorocaba, Elaine@ufscar.br
“Evaluation of cellular responses on bee organs exposed to xenobiotics by means of histological, histochemical and immuno-histochemical methods”
Trusty?
ALTERNATIVE ECONOMIC SYSTEM
EDUCATIONCHANGE THE COMSUMPTION’S “THOUGHT”
INCOME DISTRIBUTIONCHANGE THE WORLD AS YOU KNOW!
CHALENGE IN [ECO]TOXICOLOGY
PROSPECTIVE AND RETROSPECTIVE [ECO]TOXICOLOGY
LABELING XENOBIOTICSNEUTRALIZING/MODIFY XENOBIOTICS
Do we want a labelling safety world or SAFETY WORLD?
State of Art of Morphological Biomarkers in Ecotoxicology Researches on Bees
Methods for Assessing Exposure of Human and Non-Human Biota. Edited by R.G. Tardiffand B. Goldstein (f) SCOPE 1991. Published by John Wiley & Sons LId.
Do Classical Methods of Assessing Exposure in Bees are Effective for Ecotoxicology Diagnosis?
“While some relatively reliable methods have been developed for diagnosing some of these mechanisms (e.g., insect resistance to some pesticides), methods to measure precisely biological responses to exposure need to be improved.”
State of Art of Morphological Biomarkers in Ecotoxicology Researches on Bees
“(1) Direct determination of xenobiotics at the level of cells and organs;(2) Qualitative and quantitative biochemical changes in some low- molecular and high-molecular substances; (3) Genetic changes at individual and population levels as indicators of exposure; and – (4) Effects of xenobiotics at the population level.”
Methods for Assessing Exposure of Human and Non-Human Biota. Edited by R.G. Tardiffand B. Goldstein (f) SCOPE 1991. Published by John Wiley & Sons LId.
Do Classical Methods of Assessing Exposure in Bees are Effective for Ecotoxicology Diagnosis? Mortality rate Survival rate Behavioral Studies Specific Target Organ Studies
Could be not the answer for a Silent Damage!Could be not the answer for a Slow Damage Process!
Could be not the answer for Secondary Damage Effect!
BEES HAVE COMPENSATION SYSTEM!
State of Art of Morphological Biomarkers in Ecotoxicology Researches on Bees
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0132349
Classical Methods
Morphology of Specific Target
Organs
Morphology of Secondary or Optimize
Stressor Organ
HEART
THORAXHEAD ABDOMEN
Hepato-Nephrocitic SystemSinus Pericardium
Myogenic Region of the Dorsal Vessel
Hemolymph (+ xenobiotics)
Pericardial Cells
Fat Body (enocyte and trophcyte)
Hemocytes
Ostiole
Hemolymph
50µm
Pericardial Cells
Trophocytes
Enocytes
Bombus morioCourtesy: Felipe Lissoni de Andrade Nogueira
Ostiole
Valve of the Myogenic Region
Valve of the Myogenic Region
Hemolymph (+ xenobiotics)
Thank you all!
Special Thanks for Dr. Monica Jones Costa
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