biology: a molecular approach, laboratory welcome!
Post on 05-Jan-2016
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Biology: A Molecular Approach, Laboratory
Welcome!
Today’s Plan
• Orientation• Laboratory Notebooks• The Scientific Method• Controlled Experiments
ORIENTATION
Introductions - Instructors• Dr. Jackie Crisman
• Lecture
• Dr. Ellen Lehning
• Lab• Dr. Susan Greenwood
• Laboratory Technician
Introductions - Students• Name• Why you are in this class• Any thing else you’d like to tell us
Introductions - Biotechnology• Use a marker to put your name on a
name tag• Count-off from 1 to 7
• All 1s, 2s, 3s, etc. get into a group• As a group, answer the following
question:• What is biotechnology?
• Pick someone to be your spokesperson
• I’ll call on each group’s spokesperson to share their answer
Our Focuses• Research, research, research!
• Technique, technique, technique!
• Control, control, control!
• Professional but fun!
Syllabus and Tentative Schedule
Laboratory Safety Contract• Read the handout on Laboratory Safety
• Tear-off the bottom strip, sign, and turn-in the Laboratory Safety Contract
• Note the location of the following safety equipment• Glass waste, Fire extinguishers,
Eyewashes, First aid kit, Exits, Phone
Laboratory Supplies• We are supplying you with:
• Lab coats• Lab safety glasses• Gloves• Lab notebooks• All handouts
• You need to bring a calculator
Professional Conduct
• Read through the Professional Conduct Rubric
LABORATORY NOTEBOOK
Laboratory Notebook Preparation• You will work in your groups for the
rest of the semester• You will fillout your laboratory
notebook together as a group• Read the handout on Laboratory
Notebook Preparation• Read the Lab Notebook Rubric• Get the front of your notebook, title
page, and table of contents setup• Use the PCR example provided as a
reference for the rest of the semester
THE SCIENTIFIC METHOD
The Scientific Method
FundingCommunicate
CONTROLLED EXPERIMENTS
Controlled Experiments
All samples handled as nearly as humanly possible exactly the same way
Use positive controls to eliminate false negatives Use negative controls to eliminate false positives Ensures results are true!
Controlled Experiments
Read the “Bad Boys” handout Answer the following questions?
What DNA samples were collected and analyzed?
Why were they collected and analyzed? I am randomly going to call on one group and one
member of that group to answer these questions
Controlled Experiments
Get out the Using Controls in an Experiment handout
Work through problems 3 – 7 I am randomly going to call on one group and one
member of that group to answer these questions
To Print
• Syllabus• Tentative Schedule• Laboratory Safety
Contract• Professional Conduct
Rubric• Lab Notebook
Preparation• Lab Notebook Rubric• PCR notebook example• How to Design a Properly
Controlled Experiment• Practice with Controls
To Collect
• Name tags/Markers• Lab books• Chime and selector
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