biological frameworks for engineers
Post on 01-Oct-2021
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ME 498 / ME 599
Biological Frameworks
for Engineers
Class Organization
• Session 9 – Immunology
– Shirin Feghhi
• Lab 2 – Lab-on-a-Chip
– Friday 2:30-4pm
– More 320
ME 498 / ME 599
Decoding Proteins and
Protein Functions
A cell is a crowded place…
Protein Purification
Gel Electrophoresis
• Separation by charge-
to-mass ratio
• Small proteins migrate
faster than large ones
• SDS-PAGE
– Sodium dodecylsulfate
– Polyacrylamide Gel
Electrophoresis
2-D Gel Electrophoresis
Chromatography
Western Blot
Mass Spectrometry
All together now…
Cryo-EM
E. coli Ribosome
NMR Spectroscopy
Protein Function
• Pharmacological inhibitors
• Recombinant DNA
• Knock-out mice
• RNA Interference
Questions ?
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