basics of chromatography separation of analytes type of equilibrium
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Basics of Chromatography
• Ability to dissolve in mobile phase
• Interaction with stationary phase
• Time on X axis• Response on Y
Basics of Chromatography
• Capacity Factor
• Selectivity Factor
M
SA
V
VKk '
M
MR
t
ttk
'
'
'
A
B
k
k
MAR
MBR
tt
tt
)(
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