average % primer efficiency:
Post on 30-Dec-2015
26 Views
Preview:
DESCRIPTION
100. 75. Relative mRNA expression. 50. 25. 0. Tspan5. Tspan10. Tspan14. Tspan15. Tspan17. Tspan33. Average % primer efficiency:. 92.0. 111. 94.3. 96.7. 98.6. 110. - PowerPoint PPT Presentation
TRANSCRIPT
Supplementary Figure IV. Validation of mouse TspanC8 tetraspanin RT-PCR primers. The relative mRNA levels of TspanC8 tetraspanins were measured by RT-PCR from a mixture of cDNAs from mouse brain, heart, kidney, liver, lung, muscle and thymus tissue. Standard curves were used for each primer and primer efficiencies obtained using the MxPro RT-PCR software from Stratagene (error bars represent the standard error of the mean from two experiments, each done in duplicate).
Average % primer
efficiency: 92.0 111 94.3 96.7 98.6 110
100
50
0
75
25Ts
pa
n5
Tsp
an
10
Tsp
an
14
Tsp
an
15
Tsp
an
17
Tsp
an
33
Rel
ati
ve
mR
NA
exp
ress
ion
top related