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Colossolactone H, a new Ganoderma triterpenoid exhibits cytotoxicity and potentiates efficacy of gefitinib in lung cancer therapy

Su-Yu Chena, Chao-Lin Changa,b, Teng-Hai Chenc, Ya-Wen Changa,* and Shwu-Bin

Lina, c,*

aDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of

Medicine, National Taiwan University, Taipei, Taiwan, Republic of China

bFood Industry Research and Development Institute, Hsinchu, Taiwan, Republic of

China

cTrineo Biotechnology Co., LTD, New Taipei City, Taiwan, Republic of China

*Corresponding author: Dr. Shwu-Bin Lin or Dr. Ya-Wen Chang.

Fax: 886-2-23711574. E-mail: sblin@ntu.edu.tw or ywchang364@ntu.edu.tw

Keywords: Ganoderma colossum; Triterpene dilactone; Cytotoxicity; Oxidative

damage; Complementary therapy; Lung cancer

Chemical compounds studied in this article

Colossolactone G (PubChem CID: 10187119); Gefitinib (PubChem CID: 123631);

Glutathione (PubChem CID: 124886)

Abbreviations:

colo H, colossolactone H; GSH, glutathione; GO; gene ontology; ROS, reactive

oxygen species

List of Supplementary material Fig. S1 UV (A) and CD (B) spectra (MeOH) of Colo H. The UV spectrum was analyzed at 50 M and CD spectra at 3.6 M, 5.4 M, 7.2 M and 14.4 M.Fig. S2 IR Spectrum (MeOH) of Colo H.Fig. S3 1H NMR (500 MHz, CD3OD) Spectrum of Colo H. (A) Scale: 0 ppm to 10 ppm. (B) Scale: 0.8 ppm to 3.6 ppm. (C) Scale: 4 ppm to 7.2 ppm.Fig. S4 13C NMR (125 MHz, CD3OD) Spectrum of Colo H.Fig. S5 COSY Spectrum (CD3OD) of Colo H.Fig. S6 Key HMBC () (A) and NOE () (B) correlations of Colo H.Fig. S7 HMBC Spectrum (CD3OD) of Colo H.Fig. S8 HSQC Spectrum (CD3OD) of Colo H.

A

B

Fig. S1 UV (A) and CD (B) spectra (MeOH) of Colo H. The UV spectrum was analyzed at 50 M and CD spectra at 3.6 M, 5.4 M, 7.2 M and 14.4 M.

Fig. S2 IR Spectrum (MeOH) of Colo H.

A

B

C

Fig. S3 1H NMR (500 MHz, CD3OD) Spectrum of Colo H. (A) Scale: 0 ppm to 10 ppm. (B) Scale: 0.8 ppm to 3.6 ppm. (C) Scale: 4 ppm to 7.2 ppm.

Fig. S4 13C NMR (125 MHz, CD3OD) Spectrum of Colo H.

Fig. S5 COSY Spectrum (CD3OD) of Colo H.

A

B

Fig. S6 Key HMBC () (A) and NOE () (B) correlations of Colo H.

Fig. S7 HMBC Spectrum (CD3OD) of Colo H.

Fig. S8 HSQC Spectrum (CD3OD) of Colo H.