affinimeter & isothermal titration calorimetry

for the complete Thermodynamic and Kinetic Characterization of molecular interactions

(1) AFFINImeter Scientific & Development team, Software 4 Science Developments, S. L. Ed. Emprendia. �(2) Dept. of Applied Physics, Fac. of Physics, USC, Campus Vida, Santiago de Compostela, A Coruña 15782, Spain.

(3) Biophysics & Structural Biology team, IBMC, UPR9002 du CNRS 15 rue René Descartes, 67084 Strasbourg, France. Website: www.affinimeter.com; E-mail: e.munoz@affinimeter.com

AFFINImeter & Isothermal Titration Calorimetry

1.- GET THERMODYNAMIC AND KINETIC PROFILES OF YOUR INTERACTION IN A FEW CLICKS

AUTOMATIC RAWDATA PROCESSING

BASELINE CORRECTION &NOISE ELIMINATION

Thermodynamic profileKA = (2.04 ± 0.25)·106 M-1

∆H = (-9.34 ± 0.13) Kcal·mol-1

* KinITC: AN EXCLUSIVE TOOLTO GET KINETIC INFORMATION

THE MODEL BUILDER

An easy to use tool to design your own tailored binding models

GLOBAL FITTING

Perform various experiments and fit globally theresulting isotherms to get the most reliable result

A PERFECT MATCH

Eva Muñoz1, Angel Piñeiro1,2, Juan Sabin1, Javier Rial1, Philippe Dumas3, Eric Ennifar3

2.- GET STRUCTURAL AND MECHANISTIC INFORMATION OF COMPLEX INTERACTING SYSTEMS

3.- QUICK ACCESS TO DATA INTERPRETATION TOOLS

PEAK INTEGRATION

WITH ASSOCIATED ERRORS

Kinetic profilekon = (2.26 ± 0.19)·104 M-1·s-1

koff = (1.11 ± 0.09)·10-2 s-1

RAW DATA

PROCESSSED DATA

ITC ISOTHERM

EQUILIBRATION TIME CURVE (ETC)

M = RECEPTOR IN CELLA = LIGAND IN SYRINGEB = COMPETING LIGAND

COMPETITIVE BINDING OF TWO LIGANDS BINDING TO A THREE SITE RECEPTOR

EQUILIBRATION TIME

LIGAND “A” INTO RECEPTOR LIGAND “B” INTO RECEPTOR LIGAND “A” INTO (RECEPTOR-LIGAND “B”)

Local Minimum

Global Minimum

FRACTION OF OCCUPIED SITES

SITE 1

SITE 2

CONTRIBUTIONS TO THE BINDING ISOTHERM

SITE 1

SITE 3

SITE 2

LIGAND INTO MULTIVALENT POLYMER

LOCAL MINIMA

LIGAND BINDING Nº OF SITES KA (M-1) ∆H (Kcal·mol-1)

Site 1 4-5 (1.93 ± 0.11)·105 -78.14 ± 11.24

Site 2 1-2 (1.68 ± 0.08)·107 -0.14 ± 11.37

Site 3 7-9 (5.30 ± 1.01)·106 -98.44 ± 1.17

SITE 3

References* a) Burnouf D, Ennifar E, Guedich S, Puffer-Enders B, Hoffmann G, Bec G., Disdier F, Baltzinger M, Dumas P (2012) JACS 134, 559-565 b) Dumas P, Ennifar E, Bec G, Piñeiro A, Sabín J, Muñoz E, Rial J, Implementation of KinITC into AFFINImeter, Malvern-MicroCal Application note KinITC: a new method for obtaining joint thermodynamic and kinetic data by isothermal titration calorimetry. (2015).