advisor : cheng-hsin chuang advisee :wan-ting su department of mechanical engineering
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Advisor : Cheng-Hsin Chuang
Advisee :Wan-Ting Su
Department of Mechanical Engineering & Institute of Nanotechnology,
Southern Taiwan University, Tainan, TAIWANDate 2012/04/06﹕
Paper Survey
A microwire sensor for rapid detection of Escherichia coli K-12 in fresh produce
Innovate Food Science and Emerging Technologies 12 (2011) 617-622
Sungkyun Kim ,Lin Lu, Jae-Hyun Chung ,Kyonghoon Lee ,Yong Li ,Soojin Jun
Outline
• Paper Survey Sensor Design Results and discussion
• Experimental Result
• Future work
Sensor Design
Fig .1.(a) Microwire sensing unit(b) Power supply and measurement equipments
Results and Discussion
DEP force and electroosmotic flow Capillary
Fig2.Regression curve for enumeration of E.coli K-12cells
Fig3.Stepwise produres for detection of E.coli K-12
Simulation Result
Fig 4. Sequential simulation of electric field strength
Experiment Results
Fig. 5. FESEM image of E.coli K-12 cells captured on the microwire.(a)Without and (b) with AC field ﹒
Experiment Results
Fig. 6. Optimization of the working frequency for detection of E.coli K-12 at 106 CFU/ml
Fig .7. Fluorescence intensity of E.coli K-12 inPBS buffer when applied with 20VPP and 3MHz
Experiment Results
Fig. 8. Selective capture of bactrial cells from E.coli K-12 internalized spinach solution:(a) FI values of E.coli K-12 in spinach and buffer solutions at different concentrations(b) FSEM image of the microwire sensor. Many spinach particles are captured on the wire surface
Outline
• Paper Survey Sensor Design Results and discussion
• Experimental Result
• Future work
Experimental MethodExperimental
Experimental Results
Flow Rate : 0.1 ml/hr
Al2O3 Concentration : 2 wt%
BSA Concentration : 1 μM
Applied Frequency : 10 kHz
Apply Voltage : 10 Vpp
Applied Time : 0 min
Applied Time : 2 min
Applied Time : 16 min
Applied Time : 30 min
Outline
• Paper Survey Sensor Design Results and discussion
• Experimental Result
• Future work
Future work
• Paper Survey
• Experiment by Different Parameter
• Experimental Sample Using Bladder Cancer Cell
• SEM Training
Thank you for your attention!!
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