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Aalborg Universitet
Butyric acid fermentation from pretreated and hydrolyzed wheat straw byC.tyrobutyricum
Baroi, George Nabin; Westermann, Peter; Gavala, Hariklia N.
Publication date:2013
Document VersionAccepted author manuscript, peer reviewed version
Link to publication from Aalborg University
Citation for published version (APA):Baroi, G. N., Westermann, P., & Gavala, H. N. (2013). Butyric acid fermentation from pretreated and hydrolyzedwheat straw by C.tyrobutyricum. Poster presented at International Conference on Industrial biotechnology –meeting the challenges, Lund, Sweden.
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Butyric acid fermentation from pretreated and hydrolyzed wheat straw by C.tyrobutyricum
Baroi, G.N , Westermann P., Gavala H.N.* Aalborg University Copenhagen (AAU-Cph), Department of Biotechnology, Chemistry and Environmental Engineering, A C Meyers Vænge 15, DK 2450 Copenhagen SV Denmark. *hng@bio.aau.dk
Background Butyric acid and its esters are used as food supplements, artificial flavours, solvent and even its potential beneficial effects in intestinal and extra intestinal diseases and sickle cell disease have been reported. It can be used for production of bio-plastics (e.g. cellulose acetate
butyrate). Another potential use of butyric acid is to produce butanol via catalytic hydrogenation. The demand of the butyric acid is approximately 50,000 ton/year. The main bottlenecks of commercialization of biological production of acids ( in overall) are: (1) utilization of cheap feed stocks
(2) Proper strain selection &/or improvement (3) Process development. Within SUPRABIO, we aim to develop a process for cost-efficient biological production of butyric acid from wheat straw.
Conclusion The developed strain ( C. tyrobutyricum) could grow in up to 80% PHWS with higher yield (> 4,4 g/g) and selectivity (>90%). The developed process increased the productivity over 200%, most probably by decreasing inhibitory effects .
Acknowledgement
The authors wish to thank the Commission of the European Communities for the financial support of this work under SUPRABIO project (FP7-cooperation project no 241640).
Food
Animal feed
Bio-fuels
Bio-chemicals
Composite materials
Crafts
Others
Pilot scale fermentation
Pretreatment (Biogasol®)
Enzymatic hydrolysis (Biogasol®)
Strain selection and development
Process development
In-situ product separation
(Jurag separation A/S technology)
Process scheme of butyric acid production from wheat straw Exploitation of wheat plant
0.32 0,34 0,35 0,36
0,47 0,39 0,37
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40% 45% 50% 55% 60% 70% 80%
% (v/v) of Pretreated and hydrolysed wheat straw (PHWS)
Butyric acid yield (g/g sugars) in different concentration of PHWS
Acetic acid yield (g/g sugars) in different concentration of PHWS
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40% 45% 50% 55% 60% 70% 80%
% of Pre-treated and hydrolysed wheat straw (PHWS)
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Time (h)
Glucose Xylose Arabinose Acetate Butyrate
PHWS pulse Feeding
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pH
Yacetate Ybutyrate Selectivity
Yields and selectivity under different pH Simultaneous uptake of glucose and xylose in PHWS
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OD
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Hours
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Glucose Butyric acid Acetic acid
Rat
e (
g/d
ay)
Remain in the fermentor
Extracted by REED
Metabolized
Growth inhibition from K+ ion concentrations (A= 6,315gK + /l; B=12,629gK + /l; C=18,944gK +/l)
Continuous in-situ separation of butyric acid
Key Outcomes
Stra
in d
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P
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