a novel extracellular vesicle isolation method used to discover … · 2020. 7. 23. · anna...

Anna Markowska Ph.D., anna.i.markowska@gmail.comShannon Pendergrast Ph.D.,

shannon@ymirgenomics.com

Contact Information

A Novel Extracellular Vesicle Isolation Method Used to Discover Urine Liver Disease Candidate Biomarkers

P. Shannon Pendergrast1, Anna Markowska1, Ashok P. Reddy2, Phillip Wilmarth2, Scott Naugler3, Yiyi Chen4, Lina Gao4, Thuy Ngo5 Charles R. Thomas, Jr.6, Christie Binder6

1. Ymir Genomics Cambridge MA, 2. Proteomics Shared Resources, OHSU, Portland, Oregon, 3. Division of Gastroenterology and Hepatology, OHSU, Portland, Oregon. 4. Department of Biostatistics, OHSU, Portland, Oregon.. 5. Knight CEDAR Technology, OHSU, Portland, Oregon 6. Radiation Medicine, Knight Cancer Institute, OHSU, Portland, Oregon

The Need for Better HCC Surveillance Screening

Urine EVs as a Convenient Source of Biomarkers: Potential and Challenges

Liver Disease Pilot Study Design

The Std of Care for HCC Surveillance, Ultrasound + AFP is inadequate due to low Sensitivity, a lack of widespread availability/expertise, and inconvenience. What is needed is a convenient screen for early HCC.

Ymir Genomics and OHSU haveperformed a pilot discovery studyfor urine EV-associated proteinand miRNA liver diseasebiomarkers.

Several interesting candidatebiomarkers have been identified.Furthermore, the study hasperfected methods for sampleprocurement, sample preparationof EV-associated biomolecules,and high-throughput biomoleculeidentification with MS and miRNAarray. Follow-up larger scalestudies are now being planned

HCC is a Growing Problem Ymatrix Overcomes the Challenges of Urine for EV Isolation with a Rapid, Low-cost Protocol

Immunoblot and Mass Spectrometry data

miRNA Array Data (Abcam Array)

Proof-of-Concept: Known and Novel Liver-selective or Liver Disease Biomarkers in

Patient Urinary Ymatrix EV Preps

Two Early Candidate miRNA HCC Biomarkers have been

identified

CD9 Normalized Candidate Levels in Pooled Group Urinary EV Preps