41 vincent vadez tli obj5 phase ii

ICRISAT – CIAT – ISRA – Univ North Carolina – NARS of different crops

Objective 5: Cross-crop issues

Activity 1: Drought phenotyping = Refining breeder tools

Proposed Workplan for Phase II

Background and rationale for that activity

No cross specie phenotyping in Phase I but comparative genomics

Different crops work on similar traits with different methods

Incomplete / unrefined breeder tool box

No clear idea of trait value on yield

Capacity building (human) on drought phenotyping needed

Jw

Water use / productivity

Water uptake

Reproduction / partitioning

What traits?

Water use / productivity

Water uptake

Reproduction and partitioning

Modeling

Sub-A

ctivity 5: Training

Proposed sub-Activities

“Breeder toolbox”

Staygreen

SPAD / SLA

CID

Grain number / Grain size

CTD

How to improve / enlarge / refine the breeder tool box?

Relation with yield Relation with essential processes whose effects can be modeled Prediction of trait / process value on yield More / better tools

“Breeder toolbox”

Staygreen

SPAD / SLA

CID

Grain number / Grain size

CTD

(Link with Trait phenotyping + phenot protocols Services of MBP)

“Breeder toolbox” “Water uptake” “Water use /

Productivity”

“Reproduction / Remobilization”

Pre/post -anthesis water use

High TE

Low Gs

Stomata sensitivity to VPD

High mesophyll capacity

Staygreen

SPAD / SLA

CID

Grain number / Grain size

CTD

(Link with Trait phenotyping + phenot protocols Services of MBP)

“Breeder toolbox” “Water uptake” “Water use /

Productivity”

“Reproduction / Remobilization”

Pre/post -anthesis water use

High TE

Low Gs

Stomata sensitivity to VPD

High mesophyll capacity

Staygreen

SPAD / SLA

CID

Grain number / Grain size

CTD

“Breeder toolbox” “Water uptake” “Water use /

Productivity”

“Reproduction / Remobilization”

Pre/post -anthesis water use

High TE

Low Gs

Stomata sensitivity to VPD

High mesophyll capacity

Staygreen

SPAD / SLA

CID

Grain number / Grain size

CTD

“Right” kinetics of water uptake

Higher water uptake

“Breeder toolbox” “Water uptake” “Water use /

Productivity”

“Reproduction / Remobilization”

Pre/post -anthesis water use

High TE

Low Gs

Stomata sensitivity to VPD

High mesophyll capacity

Staygreen

SPAD / SLA

CID

Grain number / Grain size

CTD

“Right” kinetics of water uptake

Higher water uptake

Flower “tolerance” to water stress

Shorter/longer flower to maturity interval

PPI / PHI SBR / HI

“Breeder toolbox” “Water uptake” “Water use /

Productivity”

Pre/post -anthesis water use

Low Gs

Stomata sensitivity to VPD

Staygreen

“Right” kinetics of water uptake

Higher water uptake

?

?

? ?

?

Does my tool relate to yield?

How does the tool relate to a process?

“Breeder toolbox” “Water uptake”

Staygreen

“Right” kinetics of water uptake

“Breeder toolbox” “Water uptake”

Staygreen

“Right” kinetics of water uptake

Testing traits by Crop simulation modeling

75%

Median

25%

Probability of yield increase – Trait A

75%

Median

25%

Probability of yield increase – Trait B

“Breeder toolbox”

Trait B

Testing traits by Crop simulation modeling

Trait A

“Breeder toolbox” “Water uptake” “Water use /

Productivity”

“Reproduction / Remobilization”

Pre/post -anthesis water use

High TE

Low Gs

Stomata sensitivity to VPD

High mesophyll capacity

Staygreen

SPAD / SLA

CID

Grain number / Grain size

CTD

“Right” kinetics of water uptake

Higher water uptake

Flower “tolerance” to water stress

Shorter/longer flower to maturity interval

PPI / PHI SBR / HI

Testing traits by Crop simulation modeling

Water use / productivity

Water uptake

Reproduction and partitioning

Modeling

Sub-A

ctivity 5: Training

Outputs to TLII

Trait value predicted

Refined protocols More tools

Better pheno- typing data

Thank you