25-oh vitamin d2/d3 lc-ms/ms analysis kit vitamin profiling · sample chromatograms and validation...

Also available with Zivak® Multitasker Full Automated Sample Preparation AND Injection System

1.7 minutes analysis time

Sensitive separation with Zivak® 25-OH Vitamin DUHPLC column

Approximately 1000 analysis with one column

Suitable for anyLC-MS/MS System

25-OH Vitamin D2/D3LC-MS/MS Analysis Kitin Human Serum Samples

VITAMIN PROFILING

Main methods and procedures that have beenselected are based on EN ISO 13485 and 98/79/EC.

1.7 MINUTES

LC-MS/MS analysiswith high accuracy

and quantitativedetection

Properties of the analysis kitAccuracy 96 %

!

*Reference upon request

www.zivak.cominfo@zivak.com+90 212 210 62 15 +90 212 210 62 14

ZIVAK TECHNOLOGIES Eski Besiktas Cad. No 44, 34400 ISTANBUL - TURKEY

Distribuido en España por: INGENIERIA ANALITICA, S.L Tel: (+34) 902.45.6677Fax: (+34) 902.46.6677 www.ingenieria-analitica.com inf@ingenieria-analitica.com

Sample Chromatograms and Validation Results

25-OH Vitamin D2/D3LC-MS/MS Analysis Kitin Human Serum Samples

The test results have been validated with an AB Sciex API 4000 LC-MS/MS instrument in Nov. 2014.

Tested by with an accuracy of 96%

Sample chromatograms are for serum calibrator of Zivak® 25-OH Vitamin D2/D3 LC-MS/MS Analysis Kit

(µg/L)

(µg/L)

(%)

(%CV)

(%CV)

( 0-200 µg/L)

25-OH Vitamin D3

LOD

LOQ

Accuracy

Precision Intra-Assay

Precision Inter-Assay

Linearity (R2)

0.51

1.53

98.5

3.4

4.4

0.998

(µg/L)

(µg/L)

(%)

(%CV)

(%CV)

( 0-200 µg/L)

25-OH Vitamin D2

LOD

LOQ

Accuracy

Precision Intra-Assay

Precision Inter-Assay

Linearity (R2)

0.50

1.50

97.6

2.6

3.5

0.998

0.25 0.50 0.75 1.00 1.25 1.50 1.75

minutes

0

1

2

3

4

5

6383.3>257.2 [-16V]VITAMIN D3 1.06

0.25 0.50 0.75 1.00 1.25 1.50 1.75

minutes

0

1

2

3

4395.2>269.2 [-18V]VITAMIN D2 1.11

VITAMIN PROFILING

Distribuido en España por: INGENIERIA ANALITICA, S.L Tel: (+34) 902.45.6677 www.ingenieria-analitica.com inf@ingenieria-analitica.com