11 20-09 mbsb single molecule techniques.ppt

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Image from: http://www.bnl.gov/rhic/news/102307/story3.asp

Images from: Hecht Optics 4th ed. (2002)

Image from: http://www.aanda.org

Image from: http://d-d-photographics.com

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Images from: Hecht Optics 4th ed. (2002)

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Image from: http://www.olympusmicro.com/primer/techniques/fluorescence/anatomy/ix70fluorescence.html

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(photon)

e-

current out

Image from: Zhang et. al. J. Phys. B: At. Mol. Opt. Phys. 42 (2009)

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Fluorescence OutImage from: Zheng-Xing et al. ACTA BIOCHIMICA et

BIOPHYSICA SINICA. 2003, 35(4): 381-386

Excitation In

Fluorescence Out

Excitation In

Pinhole

Image from: http://www.olympusfluoview.com/theory/confocalintro.html

Fluorescence Out

102 nm

10 um

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V

Image from: Smith et al Science. 243 4888 (1989)

Image from: Deutsch Science. 240 4854 (1988)

Image from: Bensimon et. al. Phys. Rev. Lett. 74 (1995)

Image from: Kim & Larson Nucleic Acids Research. (2007)

Image from: Kim & Larson Nucleic Acids Research. (2007)

Image from: Galletto et al. Nature. 443 (2006)

Image from: Galletto et al. Nature. 443 (2006)

Image from: Galletto et al. Nature. 443 (2006)

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Image from: Hein et al PNAS. 105 38 (2008)

Image from: Hein et al PNAS. 105 38 (2008)

Confocal STEDIn vivo stained ER

Image from: Hein et al PNAS. 105 38 (2008)

Image from: Greenfield et al PLoS Biology. (2009)

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• QE of > 0.1

• > 20,000 cm-1 M-1

Table from: Gell, Brockwell & Smith Handbook of Single Molecule Spectroscopy. (2006)

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Image from: Abe et al Biochemistry. 42:51 (2003)

Image from: English et al Nat. Chem. Biol. 2:2 (2006)

Image from: English et al Nat. Chem. Biol. 2:2 (2006)

Image from: Sase et al PNAS. 94 (1997)

Image from: Sase et al PNAS. 94 (1997)

Image from: Yildiz et al Science. 27 (2003)

Image from: Yildiz et al Science. 27 (2003)

Image from: Yildiz et al Science. 27 (2003)

Image from: Liu et al Science. 322 (2008)

Image from: Liu et al Science. 322 (2008)

Image adapted from: Shirude & Balasubramanian Biochemie. 90:8 (2008)

Image from: Shirude & Balasubramanian Biochemie. 90:8 (2008)

Image from: PDB entry 3CRX Image from: Ha Biochemistry. 43 (2004)

Image from: Ha Biochemistry. 43 (2004)

Image from: http://www.aist.go.jp/

2nm 4nm

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Image from: Chen et al Biophys J. 92 (2007)

Image from: Capitanio et al. J. Opt. A: Pure Appl. Opt. 9 (2007)

Image from: Sönnichsen Plasmons in metal nanostructures. (2001)

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Image from: Sönnichsen et. al. Nature Biotechnology. 23 (2005)

Image from: Reinhard et. al. PNAS. 104:8 (2006)

Image from: Jun et. al. PNAS. 106:42 (2009)

Image from:Image from: Jun et. al. PNAS. 106:42 (2009)