allosteric enzymes -...
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![Page 1: Allosteric Enzymes - contents.kocw.or.krcontents.kocw.or.kr/document/wcu/2011/04/Bioprocess03-2.pdf · Allosteric enzymes. Some enzymes have more than one substrate binding site](https://reader033.vdocuments.us/reader033/viewer/2022043003/5f84ba50b7f30a608c3e77d0/html5/thumbnails/1.jpg)
Allosteric Enzymes
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Allosteric enzyme kinetics
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Determination of cooperativity coefficient
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Inhibited Enzyme Kinetics
� Inhibitor� Inhibitor binds to enzymes and reduces their activity.
� Irreversible inhibitor� Heavy metals form a stable complex with enzymes.� Heavy metals form a stable complex with enzymes.
� Pd, Cd, Hg, and others
� May be reversed only by using chelating agents such as EDTA and citrate.
� Reversible inhibitor� Dissociate more easily from the enzyme
![Page 5: Allosteric Enzymes - contents.kocw.or.krcontents.kocw.or.kr/document/wcu/2011/04/Bioprocess03-2.pdf · Allosteric enzymes. Some enzymes have more than one substrate binding site](https://reader033.vdocuments.us/reader033/viewer/2022043003/5f84ba50b7f30a608c3e77d0/html5/thumbnails/5.jpg)
Reversible Enzyme Inhibition
� Competitive inhibition
� Noncompetitive inhibition
� Uncompetitive inhibition
� Substrate inhibition
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Competitive Inhibition
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![Page 8: Allosteric Enzymes - contents.kocw.or.krcontents.kocw.or.kr/document/wcu/2011/04/Bioprocess03-2.pdf · Allosteric enzymes. Some enzymes have more than one substrate binding site](https://reader033.vdocuments.us/reader033/viewer/2022043003/5f84ba50b7f30a608c3e77d0/html5/thumbnails/8.jpg)
L-B Plot
![Page 9: Allosteric Enzymes - contents.kocw.or.krcontents.kocw.or.kr/document/wcu/2011/04/Bioprocess03-2.pdf · Allosteric enzymes. Some enzymes have more than one substrate binding site](https://reader033.vdocuments.us/reader033/viewer/2022043003/5f84ba50b7f30a608c3e77d0/html5/thumbnails/9.jpg)
Noncompetitive Inhibition
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![Page 11: Allosteric Enzymes - contents.kocw.or.krcontents.kocw.or.kr/document/wcu/2011/04/Bioprocess03-2.pdf · Allosteric enzymes. Some enzymes have more than one substrate binding site](https://reader033.vdocuments.us/reader033/viewer/2022043003/5f84ba50b7f30a608c3e77d0/html5/thumbnails/11.jpg)
L-B Plot
![Page 12: Allosteric Enzymes - contents.kocw.or.krcontents.kocw.or.kr/document/wcu/2011/04/Bioprocess03-2.pdf · Allosteric enzymes. Some enzymes have more than one substrate binding site](https://reader033.vdocuments.us/reader033/viewer/2022043003/5f84ba50b7f30a608c3e77d0/html5/thumbnails/12.jpg)
Uncompetitive Inhibition
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L-B Plot
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Substrate Inhibition
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Substrate Inhibition
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L-B Plot
![Page 20: Allosteric Enzymes - contents.kocw.or.krcontents.kocw.or.kr/document/wcu/2011/04/Bioprocess03-2.pdf · Allosteric enzymes. Some enzymes have more than one substrate binding site](https://reader033.vdocuments.us/reader033/viewer/2022043003/5f84ba50b7f30a608c3e77d0/html5/thumbnails/20.jpg)
L-B Plot