algal biofuels & bioproducts in a cold climate - a ... · algal biofuels & bioproducts in a...
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Département de microbiologie et immunologie
Université de Montréal
Dr. Patrick C. Hallenbeck
Algal Biofuels & Bioproducts
in a Cold Climate - A Canadian
Perspective
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Proposed Advantages Downsides-Can be grown on marginal
lands, urban areas or industrial
parks: no competition with food
production
-cultivation much more
technologically challenging than
traditional crops
-Rapid growth under optimal
conditions
-optimal conditions, pH, temp,
pCO2, light intensity difficult to
maintain
-relatively easily over-run by
“weeds”
-subject to plagues of “pests”
Good points and Bad points
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Proposed Advantages Downsides-High lipid content -only a few species and under
stringent conditions
-High diversity of fuel
quality/characteristics
-Sequester or mitigate CO2
emissions from fossil fuel power
plants
-“Enron style” repo 101
accounting, CO2 immediately
released when fuel combusted
-Production possible throughout
the year
-Low productivity during winter
months, heating may be
necessary
Good points and Bad points
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High lipid contentUnder the right conditions some algae contain high [TAG]
Species Rich
media
Nitrogen
deficient
Chlamydomonas
applanata
18% 33%
Chorella emersonii 29% 63%
Chorella minutissima 31% 57%
Chorella Vulgaris 18% 40%
Ettlia oleoabundans 36% 42%
Scenedesmus obliquos 12% 27%
Selenastrum gracile 21% 28%
Enhancement of lipid production in
different microalgae.
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•At saturating CO2
fixation rates, fixed
CO2 can be used
for cell growth or
lipid production,
not both
•Best strain needs
to optimize both,
trade-off
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What are some of the limiting
factors?
•Limitations in photosynthetic efficiencies
•High growth rate versus high lipid content
•Others; havestability, [CO2] needed for optimal
growth, etc.
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Challenges
•Low temperatures
•Short growing season/Low light
You can’t grow algae in the
North, its too cold and there
isn’t enough sunlight!
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Canadian agriculture- a
success inspite of the climate
challenge
Crops (algae) adapted to
local conditions need to be
grown!!
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296 x 103 m3
Algae ponds in action
in the Far North!!
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Low temperatures:
Possible solutions
•Find algal strains
that are
productive at low
temperatures
•Co-locate
production
facilities with
sources of waste
heat
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Short growing season/Low light
possible solutions
•Adopt three season
growth –low OPEX/CAPEX
required
•Use mixotrophic/
heterotrophic growth
•Need to select strains
with proper
characteristics
annual
July
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Characterization of microalgae native to Québec for biofuel production
Laboratoire de développement des
biocarburants avancées
Département de Microbiologie et
Immunologie
Faculté de Médecine
Université de Montréal
Laboratory for the development of
advanced biofuels
Department of Microbiology and
Immunology
Faculty of Medecine
University of Montreal
Patrick C. Hallenbeck
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Sampling Locations
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Traditional Isolation of Microalgae Species
2. Direct plating
1.Enrichment in liquid media
3. transfer to index plates
4. transfer to single plates
(Unialgal Strains)
Result per one
site
~ 100 Strains
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FACS
High throughput Isolation of Microalgae
Individual cells selected on the
basis of fluorescence profile
Advantage: if there is growth in a well it is
unialgal
Disadvantage: Not all survive (diatoms)
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High degree of diversity obtained, strains
show many different, interesting
characteristics
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Screening for Biomass Production
(Growth)Use of Plate Reader
Determine biomass (OD750)
Determine Biomass Using Chlorophyll measurement
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Characterization : Growth dominance
Defined Media
Waste water
Different
temperatures
Different salinities
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Characterization : Growth dominance
Result per one site
~ 100 Strains
Medium
8 °C 22 °C
Waste Water
8 °C 22 °C
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Some strains grow
well on wastewater,
either 8C or 22C
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Selection of perfoming strains using CO2
enrichment
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Start CO2
Start CO2
Stop CO2
Growth rateIsolation with
[CO2]
Isolation without
[CO2]
B17 2.03 1.06 B9
A19 1.48 0.85 A7
A11 1.37 0.72 B10
A14 1.21 0.66 A6
A17 1.17 0.65 A8
Avg * 1.26 0.66 Avg *
Selection of perfoming strains using CO2
enrichment
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Evaluation of the capacity of microalgae
native to Quebec to use xylose or
glycerol as alternative carbon source
Four strains
showing different
patterns of
response:
PCH 36 mobilizes
both substrates
PCH25 is
indifferent
PCH05 and PCH06
show apparent
inhibition
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Re
lati
ve
flu
ore
sc
en
ce
Evaluation of the capacity of microalgae native
to Quebec to use xylose or glycerol as
alternative carbon source
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Strategies
Strains like PCH05
•Could be used for hetertrophic conversion of cheap
or no cost fixed carbon substrates to lipid
•Could be used in a two stage process:
-Autotrophic growth to obtain sufficient biomass
-Second stage feeding with direct conversion of
fixed carbon to lipid with little or no increase in
biomass
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Challenges Workarounds-Large amounts of water needed -Use wastewater or brackish
-Small size, difficult to harvest -Develop novel harvesting
technologies
Screens (large species)
Natural sedimentation
Add flocculating agents
-High water content, dewatering
challenging
-Develop novel downstream
processing
• Novel extraction strategies
In situ transesterification
Engineered strains
-Require high levels of nutrient
input
-Use waste water, agricultural
run-off
What can be done?
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Dr. Simon Barnabé (Université de Québec à Trois-Rivières) is heading a project: RTA-UQTR-AlgaLabs-CSPP
27
Why does RTA consider algal biomass as a source of
renewable energy and fuel ?
• Difficulty in securing adequate supply of forest biomass
despite its abundancy in the area
• Why not produce its own biomass by growing algae using its
waste nutrients and waste energy ?
A way to secure biomass
Partnership with an algae producer is needed
Local synergy with cheap C source & nutrient suppliers
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A co-location multi-sectorial approach
28
Smelter plant
Biofuel & coproducts
Combustion
Biogas Digestion
lipid rich algal biomass
Pulp & paper technologies
(revitalizing local infrastructures)
Local C sources
CO2, nutrients, energy
Heterotrophic mixotrophicproduction
Hydrothermal treatment using
reactive extrusion
PyrolysisUpgraded
bio-oil
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Research program
29
Objective 1 Objective 2
Alga-Fuel TM
10m3 tank
Combustion, biofuel, biogas(objective 4)
Biomass Oil & residues
Algal biomass
Objective 3
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Preliminary results
30
• Adapted and robust native algal strain
• Growth medium using smelter wastewater
and in situ and local C sources
• Still looking for abundant and locally available C sources
• Harvesting and dewatering experiments in progress
• Production in Alga-FuelTM 10m3 tank starting soon (Dec 2012)
Productivity
Biomass 0,97 g∙L-1∙j-1
Neutral lipids 0,03 g∙L-1∙j-1
% lipids 13%
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36
Polar Lipids
Neutral Lipids
CO2 Induced Solvents: Overcoming the Bio-Oil Extraction Challenge
Water
Water
Water
Water
Jessop PG, Subramaniam B Chem Rev 2007, 107, 2666.Ford JW, Janakat ME, Lu J, Liotta CL, Eckert CA J. Org Chem 2008, 73, 3364.Phan L, Brown H, Peterson T, White J, Hodgson A, Jessop PG Green Chem 2009, 11, 53.
CO2 Induced Solvents• Switchable Solvents (SHS)• Gas-expanded liquids (CXL)
CO2 Induced Solvents: Overcoming the Bio-Oil Extraction Challenge
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Thank you for your attention!
Grant support : Collaborators:Dr. Viviane Yargeau, McGill Univ.
Dr. Serge Guiot, Biotech. Res. Inst.
Drs. Pat McGinn, Steven O’Leary,
NRC