Alden Jackson Parker

21 S. Wyoming Ave., Apt 1Ardmore, PA 19003

423-368-1420aldenjacksonparker@gmail.com

Linkedin: https://www.linkedin.com/pub/alden-parker/10/733/167

Objective:

A molecular biology research position in a setting that allows me to further develop my skills and enhances my career. Ideally, I would like the opportunity to grow within a company while simultaneously finishing my Master’s degree.

Education:

University of the Sciences in PhiladelphiaM.S. – Cell Biology and Biotechnology, Fall 2013 – Present Currently attending ½ time, working full time. ⅔ through as of May 2015, May 2016 anticipated finish date

Middlesex County CollegeHuman Anatomy & Physiology I & II – Summer 2011

Colorado CollegeB.S. – Biochemistry, May 2007

Baylor SchoolH.S. Diploma, May 2003

Experience:

Pioneer Seed, a Dupont company, corn/sorghum/wheat labWilmington, DEResearch Technician9/1/11 – present• Worked on many different projects simultaneously including maize, soy, wheat, and sorghum.• Planted, maintained, harvested, and labeled bolting and anther extrusion in wheat plants in laboratory and greenhouse settings. • Made cross-breeds for both corn and wheat lines. • Harvested soybean flowers for RNA analyses.

• Wrote protocols for general lab duties including bookkeeping, corn seed chip DNA extraction, leaf disc DNA Extraction, PCR, nested primer PCR, and mutation detection with Surveyor Endonuclease (Transgenomic)• Worked with and maintained automated liquid handling laboratoryEquipment (Biomek FX)• Collected wheat pollen and determined grain size using microscopy• Purified, PCR’d, heteroduplexed, digested and screened thousands of mutagenized samples across 14 different maize genes for mutations• Performed ligation, transformation, cloning (Gateway, TOPO, sticky and blunt ended), plasmid preps, and restriction digestion on known mutants as well as on SNP candidate lines• Submitted suspected mutants and SNP candidates for sequencing and confirmed by DNA Sequence Analysis (Sequencher)• Performed Taqman qPCR and recorded results on a Safire plate reader• Performed KASP genotyping and recorded results on a Safire plate reader• Analyzed KASP results in Klustercaller• Blasted Primer sequences in GrainGenes, designed new primers using Vector NTI• Performed Genome Wide Association Mapping and QTL analysis• Designed custom probes, performed southern blot analyses on mitochondrial genes of wheat for genome mapping (DIG chemiluminescent detection) • Complied with all waste and safety guidelines according to DuPont’s policies• Performed weekly laboratory safety inspections including hazardous waste areas and safety showers• Organized equipment and supplies in lab.• Maintained laboratory machinery, solutions, reagent stocks, supplies,and general lab cleanliness.• Worked independently and as part of a team

Drexel University College of Medicine –Dept. of Bioscience, mouse lab Philadelphia, PAResearch Technician8/2/09 to 4/1/11• Organized all equipment and supplies in lab.• Made sure all supply inventories in the lab were stocked includingautoclaved glassware, pipette tips, CO2 tanks,office supplies, all chemicals, equipment, etc.• Maintained laboratory machinery, solutions, reagent stocks,and general lab cleanliness.• Wrote protocols for general lab duties including bookkeeping, genotyping, western blotting, and microscopy.• Kept track of spending budget, receipts, procurement, and reconciliation• Performed mammalian dissections on male mice, concomitantly plating testicular cells, immunostaining, and imaging.• Performed Western Blotting assays• Worked independently and as part of a team

Drexel University –Dept. of Bioscience, drosophila lab Philadelphia, PAResearch Technician2/16/09 to 7/31/09• Organized all equipment and supplies in lab.• Made sure all supply inventories in the lab were stocked includingautoclaved glassware, tips, and fly vial foams, CO2 tanks, fly food,office supplies, all chemicals, equipment, etc.• Maintained laboratory machinery, solutions, reagent stocks,transgenic drosophila lines, and general lab cleanliness.• Wrote protocols for general lab duties including making drosophilafood and autoclaving glassware and drosophila vial foams.• Kept track of spending budget, receipts, procurement, reconciliation• Performed drosophila larvae dissections and imaged CNS usingimmunofluorescence and confocal microscopy• Performed Western Blotting assays• Worked independently and as part of a team

Glaxo Smith Kline – Research and DevelopmentCollegeville, PADNA Sequencing Technologist11/5/07 to 12/16/08• Synthesized cDNA from RNA vaccine samples.• Performed DNA Purification, Polymerase Chain Reaction (PCR),amplification, and DNA Sequencing.• Maintained laboratory machinery, solutions, reagent stocks, andgeneral lab cleanliness.• Performed extensive DNA sequencing analyses with DNAsequencing assembly software• Worked with and maintained automated liquid handling laboratoryEquipment (Biomek FX)• Worked independently and as part of a team

Colorado CollegeColorado Springs, COMolecular Biology Research Assistant2/14/07 to 5/14/07• Developed a standardized, usable protocol for the sequencing ofspecific human mitochondrial DNA fragments• Performed DNA purification, Polymerase Chain Reaction (PCR)amplification, cloning, cell growth, and DNA sequencing• Prepared solutions used for chemical analyses• Maintained a clean, sterile lab environment• Was instrumental in calibrating, troubleshooting, and maintaining acapillary electrophoresis/laser fluorescence DNA sequencer• Worked independently and alongside a professor

Tzu Chi UniversityHualien, TaiwanMolecular Biology Research Assistant6/1/06 to 8/1/06• Performed qualitative and quantitative chemical analyses on theexpression of PAI-1 (plasminogen activator inhibitor-1) as a result ofchronic alcohol exposure to rat aorta cells• Purified DNA, RNA, and proteins from rat aorta cell line• Performed Northern, Southern, and Western blot analyses• Acquired images of blot analyses through film development• Cultured, grew, and passaged competent rat aorta cells• Maintained a clean, sterile lab environment including surfaces,glassware, growth plates, etc.• Prepared solutions used in chemical analyses• Led discussions (in English) on project with Taiwanese lab partnersand professor• Trained Taiwanese students in performing gel electrophoresis, blotanalyses, and preparing solutions for chemical analyses• Worked independently, in a large group, and alongside a professor

Colorado CollegeColorado Springs, COAnalytical Chemistry Research Assistant6/1/05 to 8/14/05• Performed qualitative and quantitative chemical analyses on thedetermination of capsaicin in unknown samples using High Performance Liquid Chromatography (HPLC)• Prepared packing material for HPLC column by molecularlyimprinting a polymer with capsaicin• Acquired images of the molecularly imprinted polymer using microscopy• Was instrumental in calibrating and maintaining the HPLC• Maintained a clean lab environment• Worked independently and alongside a professor

References:

Ajay Sandhu, Ph.D., Research Scientist, Pioneer, a DuPont Companyajay.sandhu@pioneer.com(302) 695-6970

Stephen Sowinski, M.S., Sr. Research Associate, Pioneer, a DuPont Companystephen.g.sowinski@cgr.dupont.com(302) 256-2591

Petra Wolters, Ph.D., Sr. Research Scientist, Pioneer, a DuPont Companypetra.wolters@cgr.dupont.com(302) 695-8862

Mark Williams, Ph.D., Sr. Research Scientist, Pioneer, a DuPont Companymark.e.williams@cgr.dupont.com(302) 540-6023

Scott Davis, M.S., Sr. Research Associate, Pioneer, a DuPont Companyscott.b.davis@cgr.dupont.com(302) 695-2688

Mark Jung, M.S., Research Scientist, Pioneer, a DuPont Companymark.t.jung@cgr.dupont.com(302) 695-2618

Ada Ching, M.S., Sr. Research Associate, Pioneer, a DuPont Companyada.s.ching@cgr.dupont.com

Felice Elefant, Ph.D., Associate Professor, Drexel Universityfe22@drexel.edu(215) 895-0220

Ganesh Sathe, Ph.D., Manager of DNA Sequencing, GlaxoSmithKline USAganesh_m_sathe@gsk.com(610) 917-5315

Ralph Bertrand, Ph.D., Professor, Colorado Collegerbertrand@coloradocollege.edu(719) 389-6402

Chen, Hong-Chi, Ph.D., Professor, Tzu Chi University, Hualien, Taiwanhhc117@mail.tcu.edu.tw