180 ALCOHOL-INDUCED LIVER INJURY FOLLOWING JEJUNOILEAL BYPASS (JIP) IN RATS: CHANGES IN HEPATIC CONTENTS OF CARNITIN; COENZYME A (CoA) AND THEIR ACYLESTERS Ch. Bode, B. Brauner, J.Ch. Bode Department of Internal Medicine, Robert-Bosch-Krankenhaus, Stuttgart, F.R.G.

In a recent study we could demonstrate that alcohol-feeding (AF) in rats with JIB leads to marked fatty l iver and other signs of l iver injury (2nd ISBRA Meeting, Helsinki, June 1986). The aim of the present study was to c la r i fy whether changes in the hepatic content of carnit in (Carn.), CoA and their acylesters occur in this model of alcoholic l iver injury. Material and methods: Male Wistar rats (SPF) were subjected I) to a 90 % JIB or 2) to a sham-operation (SO). Groups (n=6-7) of JIB and SO were fed a) a l iquid diet with 36 % of alcohol (AD) or b) a diet were alcohol was replaced isocalorical ly by starch (CD, pair-feeding). After 4 months the l iver was removed in ether anesthesia for extraction and estimation of free CoA, free Carn. and their acetyl-(Ac) and acylesters (short-chain=Sc,long-chain=Lc).

Results: I) AF in SO did not s igni f icant ly influence the content of free CoA, free Carn. ~ r Ac- or acylester. 2) In the l iver of animals with JIB receiving CD Carn. and i ts Sc-esters decreased s igni f icant ly when compared to SO (free -45%, Ac -40%, Sc -47%, pc 0.05 respectively) while LC-esters increased (+16%, n.s.) . A less pronounced decrease was observed for free CoA (-20%), Ac-CoA (-20%) and Lc-CoA (-26%, p< 0.05 respectively). 3) AF in JIB resulted in a further decrease in free Carn.(-33%, p 0.05) and an increase in Ac-Carn. (+80%, p<0.05).

Conclusion: The decrease in hepatic free Carn. and CoA and their Ac-esters might play a role in the fat ty l iver induced by AD after JIB.

181 ALCOHOL-INDUCED LIVER INJURY FOLLOWING JEJUNOILEAL BYPASS OPERATION (JIB) IN RATS: EFFECT ON ADENOSINPHOSPHATES AND PHOSPHOLIPIDS J.Ch. Bode, B. Brauner, Ch. Bode Department of In ternal Medicine, Robert- Bosch-Krankenhaus, Stu t tgar t , F.R.G.

Ina recent study we could demonstrate that alcDhol-feeding in rats with JIB leads to marked f a t t y l i v e r and other signs of l i v e r in ju ry (2nd ISBRA Meeting, Hels ink i , June 1986). The aim of the present study was to invest igate whether changes in the contents of adenosinphsphates (AP) and phospholipids (PL) might contr ibute to the development of t h i s type of experimental a lcohol ic l i v e r in ju ry .

Material and methods: Male Wistar rats (SPF) were subjected I) to a 90% JIB or 2) to a sham-operation (SO). Groups (n=6-7) of JIB and SO were fed a) a l i qu id d ie t wi th 36 % alcohol (AD) or b) a d ie t were alcohol was replaced i soca lo r i ca l l y by starch (CD, pa i r - feeding) . Af ter 4 months a sample of the l i v e r was removed by freeze-clamp technique for estimation of ATP, ADP and AMP in l i g h t ether anesthesia. Other samples were taken fo r determination of t r i g l yce r i des (T), PL and l i v e r h is to logy.

Results: I) Feeding AD in JIB caused a marked fa t accumulation (h is to logy + T content) . In rats with JIB receiving CD ATP decreased by 40% when compared to SO (p<O.O5). Feeding AD in rats with JIB lead to a fu r the r reduction in ATP (-41%,p~0.05). Total content of AP was markedly reduced by AD when compared to CD in rats with JIB (-40%, pc0 .05) . 3) Feeding AD resulted in a decrease in to ta l PL content in rats with JIB (pc 0.05) whi le the content of l ec i t h i n was only s l i g h t l y reduced (n .s . ) .

Conclusion: The resul ts support the assumption of a decreased energy supply as one possible causative factor in the pathogenesis of a lcohol ic l i v e r in ju ry fo l lowing JIB.

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