PARTICIPANT STATISTICS Chemistry Q3 2015

BASIC CHEMISTRY

Specimen 1 Specimen 2 Specimen 3 Specimen 4 Specimen 5

Name Line No. Range &Type Mean SD Range &

Type Mean SD Range &Type Mean SD Range &

Type Mean SD Range &Type Mean SD No. of Labs

Alanine Aminotransferase (ALT or SGPT)Initial Grouping by Reagent and InstrumentAbbott & Abbott Architect c, ci, i 1 78 - 116 P 97.0 1.9 142 - 212 P 176.9 3.0 9 - 14 P 11.4 1.2 92 - 138 P 115.1 2.4 100 - 150 P 124.9 2.4 28Alfa Wassermann & Alfa Wasser Axcel/Alera 2 62 - 93 P 77.6 2.6 117 - 176 P 146.3 3.7 8 - 12 P 10.1 2.5 74 - 111 P 92.2 3.3 83 - 125 P 104.0 3.7 27Beckman Coulter & Beck Coult Unicel DXC 3 77 - 116 P 96.3 1.8 137 - 206 P 171.4 2.5 17 - 25 P 21.0 1.1 91 - 137 P 113.8 2.3 99 - 148 P 123.4 2.0 25Beckman Coulter & Beck Olym AU 400/600/5400 4 66 - 99 P 82.8 0.8 121 - 181 P 150.8 1.3 8 - 12 P 10.3 0.4 79 - 119 P 98.8 0.8 85 - 128 P 106.5 1.1 4Beckman Olympus & Beck Olym AU 400/600/5400 5 66 - 99 P 82.9 2.8 121 - 182 P 151.3 4.1 8 - 12 P 9.8 0.8 79 - 119 P 98.9 2.6 86 - 128 P 107.0 3.0 35Beckman Olympus & Beck Olympus AU 2700 6 69 - 103 P 85.8 1.5 125 - 188 P 156.3 4.1 9 - 13 P 10.8 0.4 82 - 123 P 102.3 3.0 89 - 133 P 111.0 3.3 4Beckman Olympus & Beckman AU 480 7 67 - 101 P 84.0 2.6 122 - 183 P 152.6 5.3 8 - 12 P 10.1 0.7 81 - 121 P 100.9 2.9 86 - 129 P 107.8 4.2 14Carolina & Beckman Synchron CX3/7/9/L 8 75 - 113 P 94.3 2.4 135 - 203 P 169.3 4.8 14 - 20 P 17.0 1.1 89 - 134 P 111.6 2.0 97 - 145 P 121.0 2.7 7Horiba ABX & Horiba ABX 400 9 83 - 124 P 103.7 3.6 152 - 228 P 189.9 4.4 11 - 16 P 13.4 0.8 99 - 148 P 123.4 3.5 107 - 161 P 134.2 3.5 9Ortho Vitros & Ortho Vitros 3600, 5600 10 83 - 124 P 103.6 3.2 148 - 221 P 184.5 3.7 18 - 28 P 23.1 1.7 94 - 141 P 117.5 3.6 110 - 165 P 137.3 6.1 9Ortho Vitros & Ortho Vitros 5,1 FS 11 85 - 127 P 105.8 2.5 148 - 222 P 185.2 4.1 20 - 30 P 24.6 2.3 94 - 141 P 117.3 2.7 109 - 163 P 135.7 3.8 6Ortho Vitros & Ortho Vitros not DT or ECi 12 82 - 123 P 102.7 4.2 146 - 219 P 182.2 3.6 18 - 27 P 22.5 2.5 93 - 140 P 116.3 3.6 108 - 163 P 135.6 2.9 33Roche Cobas & Roche Cobas Integra 13 73 - 110 P 91.7 1.5 134 - 202 P 168.0 3.0 9 - 13 P 10.7 0.7 87 - 130 P 108.5 1.9 96 - 143 P 119.4 2.4 14Roche Cobas & Roche e/c, 1XX, X000, Elec series 14 74 - 111 P 92.9 2.8 136 - 203 P 169.5 4.0 9 - 14 P 11.3 0.9 88 - 132 P 110.0 3.4 96 - 143 P 119.4 3.8 29SDI Biomed & SDI CA-240, 480 15 67 - 100 P 83.7 5.3 120 - 179 P 149.4 12.5 11 - 16 P 13.2 2.4 80 - 120 P 100.2 9.5 84 - 126 P 104.9 8.1 11Sekisui & Beck Olym AU 400/600/5400 16 74 - 111 P 92.4 1.9 133 - 199 P 166.0 4.3 9 - 14 P 11.8 1.2 86 - 129 P 107.8 1.9 94 - 142 P 118.0 3.4 5Siemens ALTI & Siemens Dim RxL, RxL Max 17 82 - 124 P 103.0 5.5 146 - 220 P 183.0 3.1 12 - 18 P 15.0 2.2 97 - 146 P 121.7 6.9 107 - 160 P 133.2 6.4 6Siemens ALTI & Siemens Dimension 18 85 - 128 P 106.3 1.9 156 - 234 P 194.8 3.3 15 - 23 P 19.0 0.0 101 - 152 P 126.5 1.7 111 - 167 P 139.3 1.9 4Siemens ALTI & Siemens Dimension EXL 19 82 - 123 P 102.5 3.8 149 - 224 P 186.8 6.9 14 - 21 P 17.3 1.2 98 - 147 P 122.3 4.7 106 - 160 P 133.1 4.9 45Siemens ALTI & Siemens Dimension Xpand 20 82 - 123 P 102.3 3.7 149 - 224 P 186.3 6.4 14 - 21 P 17.3 1.5 96 - 145 P 120.5 4.9 106 - 158 P 132.0 4.7 22Siemens Dimension & Siemens Dim RxL, RxL Max 21 84 - 126 P 104.8 2.7 149 - 224 P 186.8 2.2 16 - 24 P 20.0 0.0 99 - 148 P 123.3 1.8 106 - 160 P 133.0 2.5 4Siemens Dimension & Siemens Dimension EXL 22 83 - 124 P 103.5 3.2 149 - 224 P 186.5 5.9 15 - 22 P 18.3 1.3 98 - 147 P 122.1 3.7 106 - 159 P 132.8 3.7 13Siemens Dimension & Siemens Dimension Xpand 23 84 - 127 P 105.6 4.9 153 - 229 P 190.7 6.9 15 - 22 P 18.6 2.4 99 - 149 P 124.2 5.9 108 - 162 P 135.2 6.0 20Siemens no P5P & Siemens Advia 24 80 - 119 P 99.5 4.9 143 - 215 P 179.3 7.2 13 - 19 P 16.0 3.4 94 - 141 P 117.6 6.1 103 - 154 P 128.4 5.9 8Synermed & Synermed IR500 25 89 - 133 P 111.2 6.9 159 - 239 P 199.3 5.8 18 - 26 P 22.0 2.4 104 - 155 P 129.5 4.0 112 - 167 P 139.4 9.2 6Vital Diagnostic & Vital Diagnostic Eon 26 79 - 119 P 99.0 2.4 144 - 216 P 179.7 7.8 11 - 16 P 13.5 2.1 99 - 149 P 123.8 11.7 108 - 163 P 135.5 14.0 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 27 78 - 117 P 97.8 4.1 139 - 209 P 174.2 7.5 10 - 15 P 12.7 0.7 92 - 138 P 114.7 4.7 99 - 149 P 124.1 3.6 9Initial Grouping by ReagentAbbott 28 78 - 116 P 97.0 1.9 142 - 212 P 176.9 3.0 9 - 14 P 11.4 1.2 92 - 138 P 115.1 2.4 100 - 150 P 124.9 2.4 28Alfa Wassermann 29 62 - 93 P 77.6 2.6 117 - 175 P 146.2 3.5 8 - 12 P 10.1 2.4 74 - 111 P 92.1 3.1 83 - 125 P 103.9 3.6 30Beckman Coulter 30 75 - 113 P 94.1 5.0 134 - 201 P 167.9 7.7 15 - 23 P 19.1 3.9 88 - 133 P 110.6 6.5 97 - 145 P 120.7 6.4 35Beckman Olympus 31 67 - 100 P 83.6 2.9 122 - 183 P 152.3 4.8 8 - 12 P 9.9 0.8 80 - 120 P 99.9 3.0 86 - 129 P 107.7 3.6 55Carolina 32 75 - 113 P 94.3 2.9 134 - 201 P 167.3 8.4 13 - 19 P 15.8 2.8 88 - 132 P 109.7 5.7 95 - 143 P 118.8 6.7 17Horiba ABX 33 83 - 124 P 103.7 3.6 152 - 228 P 189.9 4.4 11 - 16 P 13.4 0.8 99 - 148 P 123.4 3.5 107 - 161 P 134.2 3.5 9Ortho Vitros 34 83 - 124 P 103.4 3.8 146 - 220 P 183.0 4.0 18 - 27 P 22.9 2.4 93 - 140 P 116.5 3.7 109 - 163 P 135.7 4.0 53Pointe Scientific 35 77 - 116 P 96.6 6.4 139 - 209 P 174.3 11.5 10 - 15 P 12.7 2.1 91 - 136 P 113.4 8.3 99 - 148 P 123.4 8.3 12Roche Cobas 36 74 - 111 P 92.7 2.8 135 - 203 P 169.3 4.3 9 - 13 P 11.1 0.9 88 - 132 P 109.7 3.4 96 - 144 P 119.7 3.6 47SDI Biomed 37 66 - 99 P 82.8 6.1 118 - 177 P 147.7 13.3 11 - 16 P 13.2 2.3 79 - 119 P 99.2 9.3 84 - 126 P 104.7 8.4 13Sekisui 38 75 - 112 P 93.2 4.4 133 - 200 P 166.8 7.7 10 - 15 P 12.4 1.7 87 - 131 P 108.8 5.5 95 - 142 P 118.7 5.7 9Siemens ALTI 39 82 - 123 P 102.7 3.9 149 - 224 P 186.8 6.7 14 - 21 P 17.3 1.5 98 - 146 P 121.9 5.0 106 - 160 P 133.1 5.1 77Siemens Dimension 40 84 - 126 P 105.1 4.3 152 - 227 P 189.4 6.7 15 - 22 P 18.6 2.0 99 - 148 P 123.6 4.9 108 - 161 P 134.4 5.1 40Siemens no P5P 41 80 - 119 P 99.5 4.9 143 - 215 P 179.3 7.2 13 - 19 P 16.0 3.4 94 - 141 P 117.6 6.1 103 - 154 P 128.4 5.9 8Synermed 42 88 - 132 P 109.6 7.5 157 - 236 P 196.6 8.6 16 - 24 P 19.8 4.9 102 - 154 P 128.0 5.2 110 - 165 P 137.7 9.3 7Vital Diagnostic 43 80 - 119 P 99.5 3.4 143 - 215 P 178.8 8.3 10 - 16 P 13.1 2.0 96 - 144 P 120.3 10.7 105 - 157 P 131.1 12.5 7Vital Diagnostics Envoy 44 78 - 117 P 97.8 4.1 139 - 209 P 174.2 7.5 10 - 15 P 12.7 0.7 92 - 138 P 114.7 4.7 99 - 149 P 124.1 3.6 9Initial Grouping by Sensitivity or PrincipleHigh moderate recovery meth 47 76 - 114 P 95.3 4.4 138 - 207 P 172.8 7.5 10 - 14 P 12.0 2.0 90 - 135 P 112.8 6.1 98 - 147 P 122.6 6.4 121High recovery methods 48 82 - 123 P 102.9 5.0 148 - 222 P 185.2 8.9 15 - 22 P 18.7 3.4 96 - 144 P 120.1 6.4 106 - 160 P 133.0 6.8 203

Low moderate recovery meths 46 70 - 104 P 86.9 2.3 124 - 186 P 155.0 3.0 11 - 16 P 13.6 2.7 81 - 122 P 101.4 3.4 89 - 133 P 111.0 3.4 7Moderate recovery methods 45 68 - 102 P 85.0 7.0 124 - 186 P 154.7 11.2 10 - 15 P 12.7 4.6 81 - 121 P 100.8 8.3 88 - 132 P 110.0 8.2 140Other 49 73 - 109 P 90.9 11.3 133 - 199 P 166.0 18.8 11 - 16 P 13.5 4.8 84 - 127 P 105.5 10.9 94 - 142 P 118.1 15.7 10Total PopulationWhole Population 50 76 - 114 P 95.3 9.7 138 - 207 P 172.6 16.8 12 - 18 P 15.3 5.1 90 - 135 P 112.2 10.9 100 - 150 P 125.3 56.3 488

AlbuminInitial Grouping by Reagent and InstrumentAbbott Architect & Abbott Architect c, ci, i 1 3.1 - 3.8 P 3.41 0.07 3.1 - 3.7 P 3.39 0.07 3.5 - 4.3 P 3.88 0.06 3.6 - 4.4 P 4.01 0.07 2.5 - 3.0 P 2.77 0.07 17Abbott BCG & Abbott Architect c, ci, i 2 3.1 - 3.8 P 3.42 0.07 3.1 - 3.8 P 3.43 0.05 3.5 - 4.3 P 3.87 0.09 3.6 - 4.5 P 4.05 0.05 2.5 - 3.1 P 2.8 0.06 6Alfa Wassermann & Alfa Wasser Axcel/Alera 3 3.2 - 3.9 P 3.52 0.09 3.2 - 3.9 P 3.53 0.07 3.5 - 4.3 P 3.92 0.08 3.8 - 4.6 P 4.17 0.09 2.6 - 3.2 P 2.94 0.06 26Beckman BCP & Beck Coult Unicel DXC 4 2.4 - 2.9 P 2.64 0.06 2.4 - 2.9 P 2.64 0.06 3.6 - 4.4 P 3.99 0.07 2.8 - 3.5 P 3.16 0.06 2.0 - 2.4 P 2.17 0.06 25Beckman Olympus & Beck Olym AU 400/600/5400 5 3.2 - 3.9 P 3.52 0.08 3.1 - 3.8 P 3.49 0.1 3.6 - 4.4 P 3.96 0.11 3.7 - 4.6 P 4.15 0.11 2.6 - 3.1 P 2.86 0.09 36Beckman Olympus & Beck Olympus AU 2700 6 3.2 - 3.9 P 3.5 0.07 3.2 - 3.9 P 3.5 0.07 3.6 - 4.4 P 4.0 0.07 3.7 - 4.5 P 4.13 0.08 2.6 - 3.2 P 2.88 0.04 4Beckman Olympus & Beckman AU 480 7 3.2 - 3.9 P 3.56 0.07 3.2 - 3.9 P 3.54 0.06 3.6 - 4.4 P 4.02 0.1 3.8 - 4.6 P 4.17 0.09 2.6 - 3.2 P 2.88 0.07 14Carolina BCP & Beckman Synchron CX3/7/9/L 8 2.4 - 2.9 P 2.65 0.15 2.4 - 2.9 P 2.65 0.13 3.5 - 4.3 P 3.92 0.13 2.9 - 3.5 P 3.22 0.16 2.0 - 2.4 P 2.17 0.07 6Horiba ABX & Horiba ABX 400 9 3.1 - 3.8 P 3.47 0.09 3.1 - 3.8 P 3.49 0.15 3.5 - 4.3 P 3.9 0.12 3.6 - 4.4 P 3.99 0.09 2.6 - 3.2 P 2.87 0.09 9Ortho Vitros & Ortho Vitros 3600, 5600 10 3.1 - 3.7 P 3.39 0.07 3.1 - 3.8 P 3.47 0.12 3.6 - 4.4 P 4.03 0.13 3.9 - 4.8 P 4.35 0.2 2.4 - 2.9 P 2.64 0.11 9Ortho Vitros & Ortho Vitros 5,1 FS 11 3.0 - 3.7 P 3.32 0.07 3.1 - 3.7 P 3.4 0.08 3.5 - 4.3 P 3.93 0.12 3.8 - 4.6 P 4.17 0.17 2.3 - 2.8 P 2.58 0.09 6Ortho Vitros & Ortho Vitros not DT or ECi 12 3.0 - 3.7 P 3.33 0.13 3.1 - 3.7 P 3.4 0.12 3.5 - 4.3 P 3.94 0.11 3.8 - 4.6 P 4.21 0.15 2.4 - 2.9 P 2.62 0.08 32Roche Cobas & Roche Cobas Integra 13 3.4 - 4.2 P 3.78 0.11 3.4 - 4.1 P 3.73 0.1 3.7 - 4.5 P 4.13 0.11 4.0 - 4.8 P 4.4 0.09 2.8 - 3.4 P 3.1 0.07 8Roche Cobas & Roche e/c, 1XX, X000, Elec series 14 3.4 - 4.1 P 3.77 0.13 3.4 - 4.1 P 3.73 0.13 3.7 - 4.5 P 4.13 0.17 4.0 - 4.9 P 4.42 0.13 2.8 - 3.4 P 3.09 0.09 10Roche Cobas BCG, gen II & Roche Cobas Integra 15 3.4 - 4.1 P 3.73 0.07 3.3 - 4.1 P 3.7 0.08 3.7 - 4.5 P 4.07 0.11 3.9 - 4.8 P 4.35 0.13 2.8 - 3.4 P 3.12 0.04 6Roche Cobas BCG, gen II & Roche e/c, 1XX, X000,Elec series 16 3.4 - 4.1 P 3.74 0.12 3.3 - 4.1 P 3.71 0.11 3.7 - 4.5 P 4.1 0.1 3.9 - 4.8 P 4.38 0.09 2.8 - 3.4 P 3.06 0.11 18

SDI Biomed & SDI CA-240, 480 17 3.3 - 4.1 P 3.69 0.14 3.4 - 4.2 P 3.79 0.12 3.7 - 4.5 P 4.13 0.14 3.9 - 4.8 P 4.35 0.17 2.8 - 3.5 P 3.14 0.12 11Sekisui & Beck Olym AU 400/600/5400 18 3.3 - 4.0 P 3.64 0.1 3.2 - 3.9 P 3.58 0.13 3.6 - 4.4 P 3.98 0.16 3.9 - 4.7 P 4.3 0.09 2.7 - 3.3 P 2.96 0.12 5Siemens Advia & Siemens Advia 19 3.3 - 4.0 P 3.63 0.11 3.3 - 4.0 P 3.63 0.07 3.5 - 4.2 P 3.85 0.07 3.9 - 4.8 P 4.33 0.08 2.6 - 3.2 P 2.91 0.03 8Siemens BCP & Siemens Dim RxL, RxL Max 20 2.3 - 2.8 P 2.51 0.06 2.3 - 2.8 P 2.52 0.06 3.6 - 4.4 P 3.98 0.08 2.7 - 3.3 P 2.99 0.07 1.8 - 2.2 P 2.01 0.06 9Siemens BCP & Siemens Dimension 21 2.2 - 2.7 P 2.45 0.13 2.2 - 2.7 P 2.48 0.13 3.5 - 4.3 P 3.87 0.18 2.6 - 3.2 P 2.94 0.13 1.8 - 2.2 P 1.96 0.09 8Siemens BCP & Siemens Dimension EXL 22 2.3 - 2.8 P 2.51 0.07 2.3 - 2.8 P 2.51 0.07 3.6 - 4.4 P 3.97 0.09 2.7 - 3.3 P 3.0 0.08 1.8 - 2.2 P 2.03 0.06 59Siemens BCP & Siemens Dimension Xpand 23 2.3 - 2.8 P 2.51 0.08 2.3 - 2.8 P 2.51 0.08 3.5 - 4.3 P 3.94 0.12 2.7 - 3.3 P 3.02 0.12 1.8 - 2.2 P 2.03 0.06 41Synermed & Synermed IR500 24 3.1 - 3.8 P 3.44 0.14 3.0 - 3.7 P 3.34 0.1 3.5 - 4.3 P 3.9 0.11 3.6 - 4.4 P 4.04 0.14 2.5 - 3.0 P 2.74 0.1 5Vital Diagnostic & Vital Diagnostic Eon 25 3.4 - 4.2 P 3.83 0.13 3.5 - 4.3 P 3.88 0.13 3.8 - 4.6 P 4.2 0.07 4.0 - 4.9 P 4.47 0.15 2.8 - 3.4 P 3.1 0.1 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 26 3.2 - 3.9 P 3.55 0.12 3.2 - 3.9 P 3.58 0.15 3.5 - 4.3 P 3.94 0.16 3.7 - 4.5 P 4.13 0.16 2.7 - 3.3 P 2.96 0.12 8Initial Grouping by ReagentAbbott Architect 27 3.1 - 3.8 P 3.41 0.07 3.1 - 3.7 P 3.39 0.07 3.5 - 4.3 P 3.88 0.06 3.6 - 4.4 P 4.01 0.07 2.5 - 3.0 P 2.77 0.07 17Abbott BCG 28 3.1 - 3.8 P 3.42 0.07 3.1 - 3.8 P 3.43 0.05 3.5 - 4.3 P 3.87 0.09 3.6 - 4.5 P 4.05 0.05 2.5 - 3.1 P 2.8 0.06 6Abbott BCP 29 2.4 - 3.0 P 2.7 0.41 2.2 - 2.7 P 2.43 0.05 3.5 - 4.3 P 3.93 0.04 2.9 - 3.5 P 3.2 0.46 1.8 - 2.2 P 1.97 0.05 4Alfa Wassermann 30 3.2 - 3.9 P 3.51 0.09 3.2 - 3.9 P 3.53 0.07 3.5 - 4.3 P 3.92 0.08 3.7 - 4.6 P 4.16 0.09 2.6 - 3.2 P 2.93 0.06 28Beckman BCG 31 3.1 - 3.8 P 3.48 0.07 3.1 - 3.8 P 3.48 0.09 3.6 - 4.3 P 3.95 0.1 3.7 - 4.5 P 4.1 0.13 2.6 - 3.1 P 2.85 0.1 6Beckman BCP 32 2.4 - 2.9 P 2.64 0.06 2.4 - 2.9 P 2.64 0.05 3.6 - 4.4 P 3.99 0.07 2.8 - 3.5 P 3.16 0.05 2.0 - 2.4 P 2.17 0.06 28Beckman Olympus 33 3.2 - 3.9 P 3.53 0.08 3.1 - 3.9 P 3.5 0.09 3.6 - 4.4 P 3.98 0.11 3.7 - 4.6 P 4.16 0.1 2.6 - 3.2 P 2.87 0.08 56Carolina BCG 34 3.1 - 3.7 P 3.4 0.1 3.1 - 3.8 P 3.44 0.09 3.6 - 4.4 P 3.96 0.07 3.6 - 4.4 P 4.0 0.09 2.6 - 3.1 P 2.85 0.1 8Carolina BCP 35 2.3 - 2.9 P 2.61 0.14 2.4 - 2.9 P 2.63 0.11 3.5 - 4.3 P 3.89 0.12 2.9 - 3.5 P 3.17 0.14 1.9 - 2.3 P 2.13 0.09 11Horiba ABX 36 3.1 - 3.8 P 3.47 0.09 3.1 - 3.8 P 3.49 0.15 3.5 - 4.3 P 3.9 0.12 3.6 - 4.4 P 3.99 0.09 2.6 - 3.2 P 2.87 0.09 9Ortho Vitros 37 3.0 - 3.7 P 3.34 0.11 3.1 - 3.8 P 3.42 0.11 3.6 - 4.3 P 3.95 0.12 3.8 - 4.7 P 4.23 0.17 2.4 - 2.9 P 2.62 0.09 51Pointe Scientific 38 3.3 - 4.0 P 3.67 0.14 3.2 - 4.0 P 3.61 0.19 3.5 - 4.3 P 3.92 0.19 3.9 - 4.8 P 4.38 0.25 2.7 - 3.3 P 2.96 0.19 11Roche Cobas 39 3.4 - 4.1 P 3.76 0.13 3.3 - 4.1 P 3.72 0.12 3.7 - 4.5 P 4.11 0.16 4.0 - 4.8 P 4.39 0.13 2.8 - 3.4 P 3.09 0.09 19Roche Cobas BCG, gen II 40 3.4 - 4.1 P 3.75 0.11 3.3 - 4.1 P 3.72 0.11 3.7 - 4.5 P 4.11 0.12 4.0 - 4.8 P 4.39 0.1 2.8 - 3.4 P 3.08 0.1 27SDI Biomed 41 3.3 - 4.0 P 3.68 0.13 3.4 - 4.2 P 3.78 0.12 3.7 - 4.5 P 4.12 0.12 3.9 - 4.8 P 4.35 0.17 2.8 - 3.4 P 3.13 0.12 13Sekisui 42 3.3 - 4.0 P 3.66 0.09 3.2 - 4.0 P 3.6 0.15 3.6 - 4.4 P 4.04 0.15 3.9 - 4.7 P 4.3 0.11 2.7 - 3.3 P 2.96 0.13 10Siemens Advia 43 3.3 - 4.0 P 3.63 0.11 3.3 - 4.0 P 3.63 0.07 3.5 - 4.2 P 3.85 0.07 3.9 - 4.8 P 4.33 0.08 2.6 - 3.2 P 2.91 0.03 8Siemens BCP 44 2.3 - 2.8 P 2.51 0.08 2.3 - 2.8 P 2.51 0.08 3.6 - 4.3 P 3.95 0.11 2.7 - 3.3 P 3.0 0.1 1.8 - 2.2 P 2.02 0.07 118Synermed 45 3.2 - 3.9 P 3.53 0.24 3.1 - 3.8 P 3.45 0.26 3.6 - 4.4 P 3.98 0.21 3.7 - 4.6 P 4.15 0.28 2.5 - 3.1 P 2.82 0.2 6Vital Diagnostic 46 3.4 - 4.1 P 3.74 0.21 3.4 - 4.1 P 3.76 0.26 3.7 - 4.5 P 4.12 0.17 3.9 - 4.8 P 4.36 0.27 2.7 - 3.3 P 3.04 0.15 5Vital Diagnostics Envoy 47 3.2 - 3.9 P 3.55 0.12 3.2 - 3.9 P 3.58 0.15 3.5 - 4.3 P 3.94 0.16 3.7 - 4.5 P 4.13 0.16 2.7 - 3.3 P 2.96 0.12 8Initial Grouping by Sensitivity or PrincipleBCG-bromcresol green 48 3.3 - 4.0 P 3.63 0.17 3.3 - 4.0 P 3.62 0.17 3.6 - 4.4 P 4.01 0.17 3.8 - 4.7 P 4.26 0.21 2.7 - 3.3 P 2.99 0.15 160BCG-rapid (60 sec or less) 50 3.1 - 3.8 P 3.45 0.13 3.1 - 3.8 P 3.46 0.12 3.6 - 4.3 P 3.95 0.11 3.8 - 4.6 P 4.17 0.15 2.5 - 3.0 P 2.77 0.14 138BCP-bromcresol purple 49 2.3 - 2.8 P 2.54 0.12 2.3 - 2.8 P 2.54 0.1 3.6 - 4.3 P 3.95 0.11 2.7 - 3.3 P 3.04 0.14 1.9 - 2.3 P 2.06 0.09 163TOTAL POPULATION 51 2.9 - 3.6 P 3.24 0.41 2.9 - 3.6 P 3.24 0.4 3.5 - 4.2 P 3.85 0.13 3.5 - 4.3 P 3.9 0.43 2.4 - 3.0 P 2.71 0.37 13

Total PopulationWhole Population 52 2.9 - 3.5 P 3.19 0.51 2.9 - 3.5 P 3.19 0.5 3.6 - 4.4 P 3.97 0.15 3.4 - 4.2 P 3.81 0.59 2.3 - 2.8 P 2.59 0.43 475

Alkaline PhosphataseInitial Grouping by Reagent and InstrumentAbbott & Abbott Architect c, ci, i 1 147 - 274 P 210.5 9.9 298 - 554 P 426.0 19.0 33 - 62 P 47.4 2.6 166 - 309 P 237.7 10.4 215 - 399 P 307.0 14.1 26Alfa Wassermann & Alfa Wasser Axcel/Alera 2 141 - 261 P 200.8 16.6 287 - 533 P 410.3 28.3 32 - 60 P 46.2 4.0 157 - 292 P 224.5 17.3 207 - 385 P 296.1 24.8 26Beckman Coulter & Beck Coult Unicel DXC 3 137 - 255 P 195.8 8.3 276 - 512 P 393.6 16.7 30 - 56 P 43.3 2.8 153 - 285 P 218.9 9.0 200 - 372 P 286.4 12.1 26Beckman Coulter & Beck Olym AU 400/600/5400 4 129 - 240 P 184.5 5.2 262 - 487 P 374.5 10.7 29 - 53 P 40.8 1.5 147 - 273 P 209.8 7.0 190 - 353 P 271.3 5.1 4Beckman Olympus & Beck Olym AU 400/600/5400 5 132 - 244 P 187.9 10.8 266 - 494 P 379.8 21.5 30 - 55 P 42.4 3.0 147 - 274 P 210.4 11.3 192 - 357 P 274.5 16.1 30Beckman Olympus & Beckman AU 480 6 134 - 248 P 191.1 10.1 274 - 508 P 391.0 18.5 30 - 56 P 43.3 3.1 150 - 279 P 214.6 12.3 196 - 363 P 279.4 15.9 14Beckman Olympus no DEA & Beck Olym AU400/600/5400 7 133 - 246 P 189.4 11.0 274 - 510 P 392.0 10.9 30 - 56 P 43.2 2.9 148 - 275 P 211.4 13.5 193 - 358 P 275.6 17.7 5

Carolina & Beckman Synchron CX3/7/9/L 8 132 - 245 P 188.1 16.7 265 - 493 P 379.0 34.3 29 - 54 P 41.5 4.0 147 - 274 P 210.5 18.4 193 - 359 P 276.1 22.7 8Horiba ABX & Horiba ABX 400 9 164 - 305 P 234.3 8.2 326 - 606 P 465.9 8.4 40 - 74 P 56.6 4.7 180 - 335 P 257.7 11.3 236 - 438 P 336.7 9.6 9Ortho Vitros & Ortho Vitros 3600, 5600 10 127 - 236 P 181.3 10.8 215 - 400 P 307.8 18.1 33 - 61 P 46.9 3.4 140 - 261 P 200.4 12.7 169 - 315 P 242.1 13.4 9Ortho Vitros & Ortho Vitros 5,1 FS 11 121 - 225 P 173.0 6.9 203 - 377 P 290.3 8.0 31 - 58 P 44.7 2.5 134 - 248 P 190.8 8.9 161 - 299 P 229.8 8.4 6Ortho Vitros & Ortho Vitros not DT or ECi 12 125 - 232 P 178.7 7.8 208 - 387 P 297.5 16.7 33 - 61 P 46.8 2.2 137 - 255 P 196.3 9.0 166 - 308 P 237.1 10.3 33Pointe Scientific & Roche/Hit. 70x/71x/73x/914 13 150 - 279 P 214.3 17.2 297 - 552 P 424.5 37.5 33 - 61 P 46.8 4.7 167 - 309 P 238.0 21.3 217 - 402 P 309.3 26.0 4Roche IFCC Gen. 2 & Roche Cobas Integra 14 147 - 273 P 210.2 4.3 296 - 550 P 423.1 10.5 35 - 65 P 49.8 2.0 165 - 306 P 235.4 6.7 215 - 400 P 307.7 10.1 13Roche IFCC Gen. 2 & Roche e/c, 1XX, X000, Elecseries 15 143 - 266 P 204.4 5.5 287 - 532 P 409.5 12.6 34 - 63 P 48.5 2.0 160 - 297 P 228.3 8.3 208 - 386 P 297.0 10.0 20

Roche/Hitachi & Roche e/c, 1XX, X000, Elec series 16 144 - 268 P 206.1 6.7 286 - 531 P 408.6 10.1 34 - 64 P 48.9 1.5 160 - 298 P 229.0 5.5 207 - 384 P 295.1 8.4 8SDI Biomed & SDI CA-240, 480 17 138 - 256 P 197.0 10.6 282 - 524 P 402.9 10.8 36 - 68 P 52.0 3.5 153 - 283 P 218.0 6.2 207 - 384 P 295.1 14.5 11Sekisui & Beck Olym AU 400/600/5400 18 161 - 299 P 230.3 21.8 328 - 610 P 469.2 43.7 36 - 67 P 51.2 4.7 180 - 334 P 256.8 23.4 235 - 437 P 335.8 29.5 6Siemens Advia & Siemens Advia 19 153 - 283 P 218.0 11.0 310 - 576 P 442.7 25.3 35 - 64 P 49.3 4.2 172 - 319 P 245.1 12.7 223 - 414 P 318.2 16.5 7Siemens Dimension & Siemens Dim RxL, RxL Max 20 158 - 294 P 225.9 8.1 319 - 592 P 455.6 16.6 37 - 68 P 52.3 2.3 178 - 330 P 254.1 9.2 232 - 430 P 330.8 10.9 9Siemens Dimension & Siemens Dimension 21 160 - 297 P 228.4 4.7 325 - 604 P 464.6 11.1 37 - 69 P 53.2 1.7 180 - 334 P 257.0 5.1 235 - 436 P 335.4 7.7 5Siemens Dimension & Siemens Dimension EXL 22 161 - 298 P 229.5 5.4 325 - 603 P 463.6 11.0 37 - 69 P 52.9 2.3 181 - 335 P 257.9 6.1 236 - 438 P 336.7 8.1 42Siemens Dimension & Siemens Dimension Xpand 23 160 - 297 P 228.8 6.0 323 - 600 P 461.2 10.6 36 - 68 P 52.0 2.6 179 - 333 P 255.8 6.0 234 - 435 P 334.3 9.5 34Siemens Dimension IFCC & Siemens Dimension EXL 24 163 - 302 P 232.5 5.3 328 - 609 P 468.8 10.1 38 - 70 P 53.8 1.4 182 - 338 P 259.7 4.8 238 - 442 P 340.1 7.0 18Siemens Dimension IFCC & Siemens DimensionXpand 25 161 - 299 P 229.7 8.1 325 - 604 P 464.4 15.5 37 - 69 P 52.8 3.6 181 - 337 P 259.2 7.7 235 - 436 P 335.1 12.2 9

Synermed & Synermed IR500 26 150 - 279 P 214.8 7.8 304 - 564 P 434.0 10.8 34 - 63 P 48.8 2.5 161 - 300 P 230.6 17.0 214 - 397 P 305.2 23.0 5Vital Diagnostic & Vital Diagnostic Eon 27 115 - 214 P 164.3 4.6 231 - 429 P 330.0 14.8 27 - 51 P 39.0 3.9 123 - 228 P 175.3 15.4 158 - 293 P 225.3 14.5 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 28 125 - 232 P 178.4 9.8 253 - 471 P 362.1 22.3 31 - 58 P 44.9 3.4 140 - 260 P 199.8 14.2 181 - 336 P 258.7 18.1 8Initial Grouping by ReagentAbbott 29 147 - 274 P 210.5 9.9 298 - 554 P 426.0 19.0 33 - 62 P 47.4 2.6 166 - 309 P 237.7 10.4 215 - 399 P 307.0 14.1 26Alfa Wassermann 30 141 - 262 P 201.4 16.2 288 - 534 P 411.0 27.6 32 - 60 P 46.3 3.8 157 - 292 P 224.7 16.8 208 - 386 P 296.6 24.1 28Beckman Coulter 31 135 - 251 P 193.2 9.6 272 - 505 P 388.8 18.9 30 - 56 P 42.9 2.7 151 - 281 P 216.1 11.3 198 - 367 P 282.6 14.7 35Beckman Olympus 32 133 - 247 P 189.7 10.5 269 - 500 P 384.5 20.7 30 - 56 P 42.8 2.9 149 - 276 P 212.4 11.6 194 - 360 P 277.1 15.8 49Beckman Olympus no DEA 33 132 - 245 P 188.7 10.2 272 - 506 P 389.0 11.4 30 - 56 P 43.2 2.7 148 - 274 P 211.0 12.4 192 - 357 P 274.3 16.4 6Carolina 34 132 - 245 P 188.6 16.1 263 - 489 P 376.3 33.5 30 - 55 P 42.4 4.7 148 - 275 P 211.5 18.3 191 - 354 P 272.6 23.8 19Horiba ABX 35 164 - 305 P 234.3 8.2 326 - 606 P 465.9 8.4 40 - 74 P 56.6 4.7 180 - 335 P 257.7 11.3 236 - 438 P 336.7 9.6 9Ortho Vitros 36 125 - 232 P 178.6 8.4 209 - 389 P 298.9 16.4 33 - 61 P 46.6 2.6 138 - 256 P 196.9 10.0 166 - 309 P 237.7 11.1 52Pointe Scientific 37 140 - 260 P 199.9 20.1 283 - 526 P 404.4 36.5 31 - 58 P 44.9 4.6 157 - 292 P 224.9 20.1 205 - 380 P 292.6 28.1 14Roche IFCC Gen. 2 38 145 - 269 P 206.8 5.8 291 - 540 P 415.3 13.5 34 - 64 P 49.0 2.0 162 - 301 P 231.2 8.3 211 - 392 P 301.4 11.2 36Roche/Hitachi 39 142 - 264 P 202.7 10.6 282 - 524 P 402.9 18.9 34 - 63 P 48.4 2.0 158 - 294 P 225.9 9.7 204 - 379 P 291.7 14.1 10SDI Biomed 40 138 - 256 P 196.8 10.1 280 - 521 P 400.6 14.5 36 - 67 P 51.3 4.0 151 - 281 P 215.8 9.2 205 - 381 P 293.1 14.3 13Sekisui 41 149 - 277 P 212.7 27.8 303 - 563 P 433.1 56.7 33 - 61 P 47.0 6.7 171 - 317 P 243.9 26.0 218 - 404 P 311.1 39.2 11Siemens Advia 42 153 - 283 P 218.0 11.0 310 - 576 P 442.7 25.3 35 - 64 P 49.3 4.2 172 - 319 P 245.1 12.7 223 - 414 P 318.2 16.5 7Siemens Dimension 43 160 - 297 P 228.8 6.0 323 - 601 P 462.0 11.8 37 - 68 P 52.5 2.4 180 - 334 P 256.7 6.5 235 - 436 P 335.1 9.1 90Siemens Dimension IFCC 44 161 - 300 P 230.6 6.8 326 - 606 P 466.3 12.3 37 - 69 P 53.1 2.9 181 - 336 P 258.8 6.1 236 - 439 P 337.8 9.2 30Synermed 45 150 - 279 P 214.8 7.8 304 - 564 P 434.0 10.8 34 - 63 P 48.8 2.5 161 - 300 P 230.6 17.0 214 - 397 P 305.2 23.0 5Vital Diagnostic 46 137 - 254 P 195.7 40.8 278 - 517 P 397.7 88.6 31 - 58 P 44.4 7.8 147 - 273 P 210.3 51.0 179 - 332 P 255.7 53.3 7Vital Diagnostics Envoy 47 125 - 232 P 178.4 9.8 253 - 471 P 362.1 22.3 31 - 58 P 44.9 3.4 140 - 260 P 199.8 14.2 181 - 336 P 258.7 18.1 8Initial Grouping by Sensitivity or PrincipleHigh moderate recovery meth 49 139 - 259 P 199.2 21.3 281 - 522 P 401.4 43.3 32 - 59 P 45.3 4.8 156 - 289 P 222.5 25.2 201 - 374 P 287.5 31.6 82High recovery methods 50 140 - 259 P 199.6 15.5 283 - 525 P 403.8 30.2 32 - 60 P 45.9 4.3 156 - 291 P 223.5 16.7 204 - 379 P 291.6 22.9 134Low moderate recovery meths 48 135 - 250 P 192.5 12.6 272 - 505 P 388.6 24.0 31 - 58 P 44.8 5.2 150 - 279 P 214.3 13.7 198 - 368 P 283.4 18.2 50Moderate recovery methods 51 133 - 247 P 190.0 22.0 240 - 445 P 342.2 67.1 33 - 62 P 47.5 4.9 147 - 273 P 210.3 25.0 183 - 340 P 261.2 38.7 80Other 52 131 - 244 P 187.7 16.7 272 - 506 P 388.9 90.8 31 - 57 P 43.9 4.7 148 - 274 P 211.0 19.2 195 - 363 P 279.1 39.4 10Very high recovery methods 53 160 - 298 P 229.1 6.4 324 - 602 P 462.8 12.5 37 - 68 P 52.6 2.7 180 - 334 P 257.2 6.5 235 - 436 P 335.6 9.6 121

Total PopulationWhole Population 54 143 - 266 P 204.8 29.4 285 - 529 P 407.0 66.0 34 - 62 P 47.9 7.3 160 - 298 P 229.2 32.1 208 - 386 P 297.0 44.7 477

Aspartate Aminotransferase (AST or SGOT)Initial Grouping by Reagent and InstrumentAbbott & Abbott Architect c, ci, i 1 99 - 148 P 123.3 1.9 168 - 251 P 209.5 2.9 52 - 77 P 64.5 1.1 103 - 155 P 128.8 1.5 134 - 201 P 167.5 2.2 4Abbott Architect & Abbott Architect c, ci, i 2 100 - 150 P 125.1 3.7 170 - 254 P 212.0 6.1 52 - 78 P 65.2 1.5 106 - 159 P 132.4 4.2 137 - 206 P 171.3 4.9 22Alfa Wassermann & Alfa Wasser Axcel/Alera 3 90 - 135 P 112.7 4.0 157 - 235 P 196.0 5.4 48 - 72 P 60.2 2.9 94 - 141 P 117.4 4.0 128 - 191 P 159.4 6.8 26Beckman Coulter & Beck Coult Unicel DXC 4 99 - 148 P 123.7 3.1 166 - 250 P 208.1 5.7 54 - 82 P 68.0 1.8 105 - 157 P 130.9 3.7 135 - 202 P 168.3 3.7 25Beckman Coulter & Beck Olym AU 400/600/5400 5 88 - 131 P 109.5 2.1 149 - 224 P 186.5 3.3 45 - 68 P 56.5 0.5 92 - 139 P 115.5 3.2 120 - 179 P 149.5 2.9 4Beckman Olympus & Beck Olym AU 400/600/5400 6 87 - 130 P 108.4 4.8 146 - 219 P 182.5 8.2 45 - 68 P 56.3 2.2 91 - 137 P 114.1 4.2 118 - 177 P 147.7 6.0 35Beckman Olympus & Beck Olympus AU 2700 7 92 - 139 P 115.5 5.2 155 - 232 P 193.3 7.6 47 - 70 P 58.3 2.9 96 - 144 P 120.3 4.0 126 - 189 P 157.3 5.9 4Beckman Olympus & Beckman AU 480 8 89 - 133 P 110.7 5.9 150 - 225 P 187.4 9.7 46 - 68 P 56.9 2.3 93 - 139 P 116.1 5.6 120 - 180 P 150.4 7.5 14Carolina & Beck Olym AU 400/600/5400 9 106 - 158 P 132.0 4.8 180 - 270 P 224.8 6.8 54 - 82 P 68.0 1.9 110 - 165 P 137.5 4.8 144 - 216 P 180.0 4.9 4Carolina & Beckman Synchron CX3/7/9/L 10 107 - 160 P 133.5 7.5 180 - 270 P 224.9 12.3 57 - 86 P 71.5 4.4 110 - 165 P 137.9 5.2 147 - 221 P 184.3 12.1 8Horiba ABX & Horiba ABX 400 11 102 - 154 P 128.1 5.6 174 - 261 P 217.4 9.9 54 - 80 P 67.0 2.8 107 - 160 P 133.7 7.0 140 - 210 P 175.2 8.7 9Ortho Vitros & Ortho Vitros 3600, 5600 12 116 - 174 P 145.3 2.3 199 - 298 P 248.4 5.7 54 - 81 P 67.1 1.0 126 - 189 P 157.8 3.8 155 - 233 P 194.3 4.7 9Ortho Vitros & Ortho Vitros 5,1 FS 13 115 - 172 P 143.5 3.9 196 - 294 P 244.7 9.2 53 - 79 P 65.7 3.0 124 - 186 P 155.0 5.2 151 - 226 P 188.5 5.9 6Ortho Vitros & Ortho Vitros not DT or ECi 14 115 - 172 P 143.2 4.4 195 - 293 P 244.0 6.8 53 - 80 P 66.5 2.2 125 - 187 P 155.7 4.5 152 - 228 P 189.7 5.0 33Roche Cobas & Roche Cobas Integra 15 99 - 149 P 124.1 3.3 170 - 254 P 211.9 5.4 51 - 77 P 64.3 1.4 103 - 155 P 129.3 3.6 137 - 206 P 171.3 4.5 14Roche Cobas & Roche e/c, 1XX, X000, Elec series 16 101 - 152 P 126.6 3.9 172 - 259 P 215.6 7.7 51 - 77 P 64.3 2.6 106 - 159 P 132.3 5.2 139 - 209 P 174.1 6.4 29SDI Biomed & SDI CA-240, 480 17 88 - 132 P 110.1 4.9 152 - 228 P 190.1 10.1 47 - 71 P 59.1 2.2 95 - 143 P 119.2 7.8 122 - 184 P 153.0 6.4 11Sekisui & Beck Olym AU 400/600/5400 18 96 - 144 P 120.4 4.1 161 - 242 P 201.6 6.8 49 - 74 P 61.4 2.9 101 - 152 P 126.6 4.8 131 - 196 P 163.6 6.2 5Siemens Dimension & Siemens Dim RxL, RxL Max 19 108 - 162 P 135.0 4.2 193 - 290 P 241.6 5.1 49 - 73 P 60.8 2.6 116 - 174 P 144.9 4.0 150 - 225 P 187.3 4.8 9Siemens Dimension & Siemens Dimension 20 110 - 165 P 137.3 2.3 196 - 294 P 244.7 4.8 49 - 74 P 61.3 1.7 117 - 176 P 146.7 3.5 152 - 227 P 189.5 3.8 6Siemens Dimension & Siemens Dimension EXL 21 106 - 159 P 132.1 4.0 190 - 284 P 237.0 5.7 49 - 73 P 60.8 2.2 114 - 171 P 142.3 3.9 147 - 220 P 183.7 4.4 51Siemens Dimension & Siemens Dimension Xpand 22 110 - 165 P 137.6 4.5 196 - 294 P 245.3 6.7 50 - 75 P 62.9 1.9 118 - 177 P 147.4 4.0 153 - 229 P 190.8 5.9 39Siemens P5P & Siemens Dimension EXL 23 107 - 160 P 133.6 3.5 192 - 287 P 239.4 7.3 49 - 74 P 61.7 2.9 115 - 173 P 143.8 3.5 150 - 224 P 186.9 5.5 9Siemens no P5P & Siemens Advia 24 106 - 160 P 133.0 5.9 179 - 268 P 223.5 7.4 58 - 86 P 71.9 4.8 112 - 168 P 139.6 5.9 145 - 218 P 181.7 6.4 8Sterling Diagnostic & Pointe Sci 180/Sterl 2000 25 89 - 133 P 111.0 2.9 135 - 203 P 168.8 9.1 40 - 60 P 50.3 3.3 94 - 141 P 117.3 7.6 112 - 167 P 139.5 9.4 4Synermed & Synermed IR500 26 111 - 167 P 139.3 6.3 185 - 278 P 231.5 13.0 59 - 88 P 73.7 4.5 114 - 171 P 142.3 9.0 150 - 225 P 187.5 10.4 6Vital Diagnostic & Vital Diagnostic Eon 27 107 - 160 P 133.3 15.6 180 - 270 P 225.3 26.4 54 - 81 P 67.5 7.4 110 - 165 P 137.5 17.3 146 - 219 P 182.3 22.9 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 28 107 - 161 P 134.0 6.7 178 - 267 P 222.7 8.3 56 - 83 P 69.4 3.4 112 - 168 P 140.4 6.4 145 - 217 P 180.9 7.5 9Initial Grouping by ReagentAbbott 29 99 - 148 P 123.3 1.9 168 - 251 P 209.5 2.9 52 - 77 P 64.5 1.1 103 - 155 P 128.8 1.5 134 - 201 P 167.5 2.2 4Abbott Architect 30 100 - 150 P 125.1 3.7 170 - 254 P 212.0 6.1 52 - 78 P 65.2 1.5 106 - 159 P 132.4 4.2 137 - 206 P 171.3 4.9 22Alfa Wassermann 31 90 - 136 P 113.1 4.0 157 - 236 P 196.6 5.5 48 - 72 P 60.2 2.8 94 - 141 P 117.1 5.0 128 - 192 P 159.9 6.7 29Beckman Coulter 32 96 - 145 P 120.5 7.1 163 - 244 P 203.3 11.3 53 - 79 P 66.1 4.4 102 - 153 P 127.3 7.8 131 - 197 P 164.2 9.4 34Beckman Olympus 33 88 - 132 P 109.6 5.5 148 - 222 P 184.8 9.1 45 - 68 P 56.7 2.3 92 - 138 P 115.2 5.0 119 - 179 P 149.2 6.9 55Carolina 34 106 - 159 P 132.8 8.5 180 - 270 P 225.2 15.0 56 - 84 P 70.1 4.5 110 - 165 P 137.3 6.3 147 - 221 P 184.0 12.4 21Horiba ABX 35 102 - 154 P 128.1 5.6 174 - 261 P 217.4 9.9 54 - 80 P 67.0 2.8 107 - 160 P 133.7 7.0 140 - 210 P 175.2 8.7 9Ortho Vitros 36 115 - 173 P 143.8 4.2 196 - 294 P 245.3 7.3 53 - 80 P 66.6 2.3 125 - 187 P 156.2 4.8 152 - 229 P 190.5 5.7 52Pointe Scientific 37 104 - 156 P 130.2 13.4 171 - 256 P 213.5 22.8 53 - 80 P 66.6 5.8 109 - 164 P 136.3 15.1 139 - 209 P 174.1 16.4 12Roche Cobas 38 100 - 151 P 125.6 3.9 171 - 257 P 214.3 7.2 52 - 77 P 64.4 2.2 105 - 158 P 131.3 5.0 138 - 208 P 173.1 5.9 47SDI Biomed 39 88 - 131 P 109.5 6.5 151 - 226 P 188.3 12.3 47 - 71 P 58.8 2.3 94 - 141 P 117.4 9.2 121 - 182 P 151.6 8.4 13Sekisui 40 97 - 145 P 120.9 5.6 163 - 244 P 203.6 10.5 50 - 75 P 62.4 3.4 102 - 153 P 127.1 6.8 131 - 197 P 164.0 8.3 10Siemens Dimension 41 108 - 162 P 134.7 4.9 193 - 289 P 240.9 7.2 49 - 74 P 61.6 2.3 116 - 174 P 144.7 4.6 150 - 224 P 187.0 6.0 105Siemens P5P 42 107 - 161 P 134.1 3.6 192 - 289 P 240.6 7.3 49 - 74 P 61.8 2.6 116 - 174 P 144.6 4.3 150 - 225 P 187.7 6.0 14Siemens no P5P 43 106 - 160 P 133.0 5.9 179 - 268 P 223.5 7.4 58 - 86 P 71.9 4.8 112 - 168 P 139.6 5.9 145 - 218 P 181.7 6.4 8Sterling Diagnostic 44 90 - 136 P 113.0 6.4 137 - 205 P 171.0 11.5 40 - 60 P 50.1 2.9 94 - 142 P 118.1 7.5 114 - 170 P 141.9 7.7 8Synermed 45 110 - 166 P 138.0 6.7 184 - 276 P 230.1 12.5 58 - 87 P 72.9 4.6 113 - 169 P 141.1 8.8 150 - 224 P 186.9 9.7 7Vital Diagnostic 46 100 - 150 P 125.4 15.4 171 - 256 P 213.4 24.6 53 - 80 P 66.3 6.8 105 - 158 P 131.6 14.8 137 - 205 P 171.0 22.1 7Vital Diagnostics Envoy 47 107 - 161 P 134.0 6.7 178 - 267 P 222.7 8.3 56 - 83 P 69.4 3.4 112 - 168 P 140.4 6.4 145 - 217 P 180.9 7.5 9Initial Grouping by Sensitivity or PrincipleAll with P5P 54 107 - 161 P 134.1 3.6 192 - 289 P 240.6 7.3 49 - 74 P 61.8 2.6 116 - 174 P 144.6 4.3 150 - 225 P 187.7 6.0 14High moderate recovery meth 49 103 - 154 P 128.5 12.4 167 - 251 P 209.1 25.8 52 - 78 P 65.2 8.8 107 - 161 P 134.2 13.2 136 - 205 P 170.6 19.5 40Low moderate recovery meth 50 92 - 139 P 115.6 8.0 157 - 236 P 196.5 13.4 49 - 73 P 61.1 5.0 97 - 146 P 121.6 8.9 127 - 191 P 158.9 10.9 144Low recovery methods 51 100 - 149 P 124.4 14.7 170 - 254 P 211.9 23.4 53 - 79 P 65.8 6.5 105 - 157 P 130.8 14.0 136 - 204 P 169.8 20.9 8Moderate recovery methods 48 100 - 150 P 125.1 9.1 170 - 255 P 212.7 15.3 52 - 78 P 65.2 4.9 105 - 157 P 130.9 8.9 138 - 206 P 172.0 12.8 116Other 52 98 - 148 P 123.1 14.1 172 - 258 P 214.7 26.7 51 - 76 P 63.6 5.8 104 - 157 P 130.5 16.7 134 - 201 P 167.9 20.8 11Very high recovery methods 53 110 - 165 P 137.7 6.3 194 - 291 P 242.3 7.5 51 - 76 P 63.3 3.3 119 - 178 P 148.5 7.1 151 - 226 P 188.2 6.1 157Total Population

Whole Population 55 102 - 152 P 127.0 12.7 174 - 261 P 217.7 24.1 50 - 76 P 63.1 5.4 107 - 161 P 134.3 14.9 139 - 209 P 174.1 25.8 490

Bicarbonate (Total CO2)Initial Grouping by Reagent and InstrumentAbbott Architect & Abbott Architect c, ci, i 1 17 - 26 P 21.5 1.1 10 - 18 C 14.2 1.3 5 - 13 C 8.8 0.9 20 - 30 P 24.6 1.2 10 - 18 C 13.7 1.0 25Alfa Wassermann & Alfa Wasser Axcel/Alera 2 19 - 28 P 23.6 2.3 12 - 20 C 16.2 1.8 6 - 14 C 9.7 1.9 22 - 32 P 27.0 2.2 11 - 19 C 15.4 2.1 26Beckman Coulter & Beck Coult Unicel DXC 3 20 - 30 P 24.6 0.9 13 - 21 C 16.8 0.6 8 - 16 C 11.7 0.8 22 - 33 P 27.3 1.5 12 - 20 C 16.0 1.1 26Beckman Coulter & Beck Olym AU 400/600/5400 4 19 - 29 P 23.8 1.6 11 - 19 C 15.0 1.6 5 - 13 C 9.3 1.5 21 - 32 P 26.5 2.1 11 - 19 C 14.8 1.3 4Beckman Coulter & Beckman AU 480 5 19 - 29 P 23.8 1.5 12 - 20 C 15.5 0.5 6 - 14 C 9.8 0.8 22 - 33 P 27.5 2.1 11 - 19 C 15.3 0.8 4Beckman Olympus & Beck Olym AU 400/600/5400 6 19 - 29 P 24.1 1.9 12 - 20 C 16.1 1.4 6 - 14 C 10.1 1.6 22 - 32 P 26.9 2.5 11 - 19 C 15.1 1.6 35Beckman Olympus & Beck Olympus AU 2700 7 19 - 29 P 24.0 0.8 12 - 20 C 15.7 0.9 7 - 15 C 10.7 0.5 22 - 32 P 27.0 0.8 11 - 19 C 15.3 0.9 4Beckman Olympus & Beckman AU 480 8 19 - 29 P 24.1 1.7 11 - 19 C 15.2 2.3 6 - 14 C 9.9 1.5 22 - 32 P 26.9 2.2 12 - 20 C 15.6 1.8 15Carolina & Beck Olym AU 400/600/5400 9 20 - 29 P 24.5 2.5 12 - 20 C 16.3 1.3 8 - 16 C 11.8 2.6 23 - 34 P 28.2 2.4 11 - 19 C 15.0 1.9 5Carolina & Beckman Synchron CX3/7/9/L 10 20 - 30 P 24.8 1.0 14 - 22 C 17.9 1.6 10 - 18 C 13.9 1.0 23 - 34 P 28.4 1.5 15 - 23 C 18.5 1.9 8Horiba ABX & Horiba ABX 400 11 19 - 29 P 24.3 2.1 12 - 20 C 16.3 1.8 7 - 15 C 10.8 1.4 21 - 32 P 26.3 1.5 12 - 20 C 15.6 1.3 9Medica & Medica Easy RA 12 19 - 28 P 23.7 1.7 11 - 19 C 15.0 2.8 7 - 15 C 10.5 1.7 19 - 29 P 24.3 3.7 10 - 18 C 13.9 2.8 7Ortho Vitros & Ortho Vitros 3600, 5600 13 18 - 27 P 22.9 1.2 11 - 19 C 15.3 1.4 5 - 13 C 9.0 0.6 20 - 30 P 25.3 1.6 9 - 17 C 13.4 2.0 7Ortho Vitros & Ortho Vitros 5,1 FS 14 19 - 28 P 23.2 1.6 11 - 19 C 15.3 1.2 5 - 13 C 8.8 0.9 21 - 31 P 25.8 1.7 11 - 19 C 15.0 1.2 6Ortho Vitros & Ortho Vitros not DT or ECi 15 19 - 28 P 23.5 1.0 12 - 20 C 15.5 0.8 5 - 13 C 9.2 1.2 20 - 30 P 25.1 1.6 10 - 18 C 14.0 1.6 23Ortho Vitros UV/unc & Ortho Vitros not DT or ECi 16 19 - 28 P 23.7 0.6 12 - 20 C 16.0 1.7 5 - 13 C 9.4 1.1 21 - 31 P 26.1 1.7 11 - 19 C 14.8 1.2 10Roche Cobas & Roche Cobas Integra 17 18 - 27 P 22.2 1.8 11 - 19 C 15.1 1.7 6 - 14 C 10.0 1.3 19 - 29 P 23.8 2.2 10 - 18 C 14.2 1.6 11Roche Cobas & Roche e/c, 1XX, X000, Elec series 18 18 - 27 P 22.3 1.9 10 - 18 C 14.3 1.7 5 - 13 C 9.0 1.2 19 - 29 P 24.3 2.3 9 - 17 C 13.3 1.7 24SDI Biomed & SDI CA-240, 480 19 17 - 26 P 21.4 1.5 11 - 19 C 14.8 1.3 7 - 15 C 10.5 1.8 19 - 29 P 24.3 1.2 11 - 19 C 14.9 2.0 10Sekisui & Beck Olym AU 400/600/5400 20 18 - 27 P 22.3 1.3 12 - 20 C 15.5 0.5 6 - 14 C 9.5 0.5 21 - 31 P 25.8 0.8 11 - 19 C 14.5 0.5 5Siemens Advia & Siemens Advia 21 20 - 30 P 24.6 1.9 13 - 21 C 17.2 1.2 6 - 14 C 10.4 0.8 23 - 35 P 28.8 1.6 11 - 19 C 14.6 2.5 5Siemens ECO2 & Siemens Dim RxL, RxL Max 22 21 - 31 P 26.1 1.4 14 - 22 C 17.9 1.6 8 - 16 C 12.0 1.8 23 - 34 P 28.5 1.9 13 - 21 C 16.9 1.3 9Siemens ECO2 & Siemens Dimension 23 21 - 31 P 25.7 2.3 12 - 20 C 16.3 2.3 7 - 15 C 11.3 2.2 22 - 33 P 27.5 2.4 12 - 20 C 16.0 1.6 6Siemens ECO2 & Siemens Dimension EXL 24 21 - 31 P 25.7 1.7 14 - 22 C 17.5 1.5 8 - 16 C 11.5 1.4 23 - 34 P 28.7 1.7 13 - 21 C 16.6 1.7 59Siemens ECO2 & Siemens Dimension Xpand 25 20 - 31 P 25.6 1.3 13 - 21 C 17.3 1.6 8 - 16 C 11.8 1.6 23 - 34 P 28.6 2.3 13 - 21 C 16.8 1.8 43Siemens tCO2 & Siemens Dimension EXL 26 22 - 33 P 27.3 2.0 15 - 23 C 18.5 1.1 8 - 16 C 12.0 1.6 23 - 35 P 29.0 2.2 13 - 21 C 16.5 1.5 4Vital Diagnostic Eon & Vital Diagnostic Eon 27 16 - 24 P 20.3 2.7 10 - 18 C 14.3 1.2 6 - 14 C 9.5 2.1 20 - 30 P 24.8 2.9 10 - 18 C 14.3 1.9 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 28 19 - 28 P 23.4 1.6 11 - 19 C 15.3 1.2 4 - 12 C 8.3 1.1 21 - 32 P 26.6 1.9 11 - 19 C 14.6 0.9 8Initial Grouping by ReagentAbbott Architect 29 17 - 26 P 21.5 1.1 10 - 18 C 14.2 1.3 5 - 13 C 8.8 0.9 20 - 30 P 24.6 1.2 10 - 18 C 13.7 1.0 25Alfa Wassermann 30 19 - 29 P 23.8 2.3 12 - 20 C 16.4 1.9 6 - 14 C 9.9 2.0 22 - 33 P 27.2 2.3 12 - 20 C 15.6 2.1 28Beckman Coulter 31 19 - 29 P 24.3 1.1 12 - 20 C 16.4 1.0 7 - 15 C 11.1 1.3 22 - 33 P 27.2 1.6 12 - 20 C 15.7 1.1 36Beckman Olympus 32 19 - 29 P 24.1 1.8 12 - 20 C 15.9 1.7 6 - 14 C 10.1 1.5 22 - 32 P 26.9 2.3 11 - 19 C 15.2 1.6 56Carolina 33 19 - 29 P 24.1 1.8 13 - 21 C 16.9 1.9 8 - 16 C 12.2 2.4 22 - 33 P 27.8 2.3 13 - 21 C 17.2 2.7 19Horiba ABX 34 19 - 29 P 24.3 2.1 12 - 20 C 16.3 1.8 7 - 15 C 10.8 1.4 21 - 32 P 26.3 1.5 12 - 20 C 15.6 1.3 9Medica 35 19 - 28 P 23.7 1.7 11 - 19 C 15.0 2.8 7 - 15 C 10.5 1.7 19 - 29 P 24.3 3.7 10 - 18 C 13.9 2.8 7Ortho Vitros 36 19 - 28 P 23.3 1.3 11 - 19 C 15.3 1.1 5 - 13 C 9.1 1.1 20 - 30 P 25.1 1.7 10 - 18 C 14.1 1.7 39Ortho Vitros UV/unc 37 19 - 28 P 23.6 0.6 12 - 20 C 15.8 1.9 5 - 13 C 9.3 1.0 21 - 31 P 26.2 1.5 10 - 18 C 14.0 2.2 13Pointe Scientific 38 17 - 25 P 21.0 2.2 11 - 19 C 14.6 1.9 5 - 13 C 9.2 2.0 20 - 30 P 25.2 2.9 10 - 18 C 14.2 1.9 7Roche Cobas 39 18 - 27 P 22.3 1.8 10 - 18 C 14.4 1.8 5 - 13 C 9.4 1.3 19 - 29 P 24.1 2.2 10 - 18 C 13.6 1.7 40Roche Hitachi 40 17 - 26 P 21.4 1.2 9 - 17 C 13.0 1.4 5 - 13 C 8.8 0.7 19 - 28 P 23.4 1.6 9 - 17 C 13.0 0.9 5SDI Biomed 41 18 - 27 P 22.3 2.6 11 - 19 C 15.3 1.7 7 - 15 C 10.5 1.7 19 - 29 P 24.1 1.7 11 - 19 C 15.3 2.0 12Sekisui 42 19 - 28 P 23.4 2.1 12 - 20 C 15.5 1.7 6 - 14 C 9.6 0.7 21 - 31 P 25.7 2.6 11 - 19 C 15.0 1.5 11Siemens Advia 43 20 - 30 P 24.6 1.9 13 - 21 C 17.2 1.2 6 - 14 C 10.4 0.8 23 - 35 P 28.8 1.6 11 - 19 C 14.6 2.5 5Siemens ECO2 44 21 - 31 P 25.7 1.6 13 - 21 C 17.4 1.7 8 - 16 C 11.6 1.5 23 - 34 P 28.6 2.0 13 - 21 C 16.7 1.7 118Siemens tCO2 45 22 - 32 P 27.0 1.9 15 - 23 C 18.5 1.0 8 - 16 C 11.8 1.3 23 - 35 P 29.0 1.8 13 - 21 C 16.5 1.3 6Vital Diagnostic Eon 46 16 - 24 P 20.3 2.7 10 - 18 C 14.3 1.2 6 - 14 C 9.5 2.1 20 - 30 P 24.8 2.9 10 - 18 C 14.3 1.9 4Vital Diagnostics Envoy 47 19 - 28 P 23.4 1.6 11 - 19 C 15.3 1.2 4 - 12 C 8.3 1.1 21 - 32 P 26.6 1.9 11 - 19 C 14.6 0.9 8Initial Grouping by Sensitivity or PrincipleAll other methods 54 21 - 31 P 25.7 1.6 13 - 21 C 17.4 1.7 8 - 16 C 11.6 1.5 23 - 34 P 28.6 2.0 13 - 21 C 16.7 1.7 118Diluted ISE results 49 19 - 28 P 23.4 2.2 12 - 20 C 15.7 1.7 6 - 14 C 10.1 1.7 21 - 32 P 26.3 2.1 11 - 19 C 15.0 1.5 81Other 53 19 - 28 P 23.4 1.4 11 - 19 C 15.2 1.9 6 - 14 C 10.1 1.0 21 - 32 P 26.8 2.0 11 - 19 C 14.8 0.8 9UV/bichromatic methods 50 19 - 29 P 24.0 2.0 12 - 20 C 16.1 1.8 6 - 14 C 10.1 1.7 22 - 32 P 26.9 2.2 11 - 19 C 15.4 1.8 93UV/kinetic (rate) methods 48 18 - 27 P 22.5 2.1 11 - 19 C 14.8 1.9 6 - 14 C 9.7 1.5 20 - 29 P 24.4 2.4 10 - 18 C 14.1 1.9 57UV/uncorrected methods 51 19 - 28 P 23.5 2.1 12 - 20 C 15.8 2.2 6 - 14 C 10.3 2.0 21 - 32 P 26.5 3.0 11 - 19 C 15.1 2.6 73Undiluted ISE results 52 19 - 28 P 23.3 1.3 11 - 19 C 15.4 1.2 5 - 13 C 9.2 1.2 20 - 30 P 25.2 1.7 10 - 18 C 14.2 1.7 40Total PopulationWhole Population 55 19 - 29 P 23.9 2.6 12 - 20 C 16.0 2.4 7 - 15 C 10.6 4.1 21 - 32 P 26.7 2.9 11 - 19 C 15.3 2.3 473

Bilirubin, TotalInitial Grouping by Reagent and InstrumentAbbott Architect & Abbott Architect c, ci, i 1 1.5 - 2.3 C 1.9 0.18 2.7 - 4.0 P 3.37 0.27 0 - 0.5 C 0.11 0.04 2.0 - 3.0 P 2.5 0.21 1.7 - 2.6 P 2.16 0.19 26Alfa Wassermann & Alfa Wasser Axcel/Alera 2 1.7 - 2.6 P 2.17 0.18 3.1 - 4.7 P 3.92 0.29 0 - 0.5 C 0.13 0.06 2.3 - 3.5 P 2.9 0.24 2.0 - 2.9 P 2.45 0.22 27Beckman Coulter & Beck Coult Unicel DXC 3 1.9 - 2.8 P 2.34 0.17 3.2 - 4.8 P 4.01 0.18 0 - 0.6 C 0.2 0.1 2.5 - 3.7 P 3.07 0.18 2.0 - 3.0 P 2.52 0.18 25Beckman Coulter & Beck Olym AU 400/600/5400 4 1.9 - 2.9 P 2.38 0.04 3.4 - 5.1 P 4.25 0.11 0 - 0.5 C 0.13 0.04 2.4 - 3.6 P 2.98 0.11 2.2 - 3.3 P 2.78 0.04 4Beckman Olympus & Beck Olym AU 400/600/5400 5 1.9 - 2.8 P 2.36 0.15 3.4 - 5.1 P 4.23 0.24 0 - 0.5 C 0.11 0.03 2.4 - 3.6 P 3.02 0.18 2.2 - 3.3 P 2.75 0.16 35Beckman Olympus & Beck Olympus AU 2700 6 1.8 - 2.7 P 2.28 0.19 3.4 - 5.1 P 4.22 0.23 0 - 0.5 C 0.1 0.0 2.4 - 3.6 P 3.03 0.18 2.2 - 3.2 P 2.7 0.21 4Beckman Olympus & Beckman AU 480 7 1.8 - 2.8 P 2.31 0.15 3.4 - 5.0 P 4.2 0.21 0 - 0.5 C 0.11 0.03 2.4 - 3.6 P 2.96 0.14 2.2 - 3.3 P 2.71 0.16 14Carolina & Beckman Synchron CX3/7/9/L 8 1.8 - 2.7 P 2.27 0.17 3.1 - 4.7 P 3.93 0.35 0 - 0.7 C 0.26 0.1 2.4 - 3.7 P 3.06 0.3 2.0 - 3.1 P 2.56 0.3 7Horiba ABX & Horiba ABX 400 9 1.6 - 2.4 C 1.99 0.14 2.9 - 4.4 P 3.66 0.18 0 - 0.5 C 0.07 0.05 2.1 - 3.2 P 2.63 0.19 1.8 - 2.7 P 2.26 0.16 9Ortho Vitros & Ortho Vitros 3600, 5600 10 1.6 - 2.5 P 2.05 0.26 3.1 - 4.6 P 3.84 0.36 0 - 0.5 C 0.1 0.0 2.3 - 3.4 P 2.84 0.32 1.8 - 2.8 P 2.3 0.21 9Ortho Vitros & Ortho Vitros 5,1 FS 11 1.7 - 2.6 P 2.15 0.18 3.2 - 4.8 P 4.0 0.24 0 - 0.5 C 0.1 0.0 2.4 - 3.5 P 2.95 0.19 2.0 - 3.0 P 2.46 0.17 6Ortho Vitros & Ortho Vitros not DT or ECi 12 1.7 - 2.5 P 2.12 0.16 3.2 - 4.8 P 4.0 0.29 0 - 0.5 C 0.11 0.02 2.3 - 3.5 P 2.9 0.22 1.9 - 2.9 P 2.42 0.18 33Roche Cobas & Roche Cobas Integra 13 1.3 - 2.1 C 1.72 0.15 2.5 - 3.7 P 3.11 0.18 0 - 0.4 C 0.02 0.04 1.8 - 2.7 P 2.28 0.18 1.6 - 2.4 C 1.98 0.13 9Roche Cobas & Roche e/c, 1XX, X000, Elec series 14 1.4 - 2.2 C 1.82 0.11 2.7 - 4.1 P 3.38 0.19 0 - 0.6 C 0.15 0.07 2.0 - 2.9 P 2.44 0.16 1.7 - 2.5 P 2.11 0.14 13Roche Cobas BILTS & Roche Cobas Integra 15 1.3 - 2.1 C 1.72 0.07 2.5 - 3.8 P 3.16 0.14 0 - 0.5 C 0.12 0.12 1.8 - 2.7 P 2.28 0.07 1.6 - 2.4 C 1.98 0.1 5Roche Cobas BILTS & Roche e/c, 1XX, X000, Elecseries 16 1.4 - 2.2 C 1.76 0.09 2.6 - 3.9 P 3.26 0.2 0 - 0.5 C 0.14 0.09 1.9 - 2.8 P 2.35 0.17 1.6 - 2.4 C 1.99 0.13 16

SDI Biomed & SDI CA-240, 480 17 2.1 - 3.1 P 2.6 0.12 3.9 - 5.8 P 4.82 0.3 0 - 0.5 C 0.09 0.03 2.8 - 4.3 P 3.55 0.17 2.3 - 3.5 P 2.89 0.14 11Sekisui & Beck Olym AU 400/600/5400 18 1.5 - 2.3 C 1.87 0.09 2.8 - 4.2 P 3.47 0.14 0 - 0.5 C 0.08 0.04 2.0 - 3.0 P 2.5 0.13 1.7 - 2.6 P 2.15 0.1 6Siemens Advia & Siemens Advia 19 1.6 - 2.4 P 2.03 0.16 3.0 - 4.4 P 3.7 0.26 0 - 0.5 C 0.05 0.05 2.2 - 3.2 P 2.7 0.19 1.8 - 2.7 P 2.28 0.17 6Siemens Dimension & Siemens Dim RxL, RxL Max 20 1.6 - 2.4 C 1.98 0.14 3.0 - 4.4 P 3.7 0.19 0 - 0.5 C 0.06 0.05 2.1 - 3.2 P 2.65 0.17 1.8 - 2.7 P 2.26 0.17 10Siemens Dimension & Siemens Dimension 21 1.4 - 2.2 C 1.84 0.12 2.7 - 4.1 P 3.41 0.26 0 - 0.4 C 0.04 0.05 2.0 - 3.0 P 2.51 0.16 1.7 - 2.5 P 2.1 0.15 7Siemens Dimension & Siemens Dimension EXL 22 1.5 - 2.3 C 1.89 0.17 2.8 - 4.2 P 3.49 0.25 0 - 0.5 C 0.08 0.05 2.0 - 3.1 P 2.56 0.18 1.7 - 2.6 P 2.14 0.17 58Siemens Dimension & Siemens Dimension Xpand 23 1.5 - 2.3 C 1.9 0.15 2.8 - 4.2 P 3.51 0.22 0 - 0.5 C 0.06 0.05 2.0 - 3.1 P 2.56 0.16 1.7 - 2.6 P 2.15 0.15 42Synermed & Synermed IR500 24 1.9 - 2.9 P 2.42 0.13 3.5 - 5.2 P 4.35 0.27 0 - 0.5 C 0.08 0.04 2.4 - 3.5 P 2.95 0.11 2.2 - 3.3 P 2.72 0.11 4Vital Diagnostic & Vital Diagnostic Eon 25 1.5 - 2.4 C 1.95 0.05 3.1 - 4.7 P 3.88 0.45 0 - 0.7 C 0.25 0.09 2.2 - 3.4 P 2.8 0.25 1.9 - 2.9 P 2.4 0.32 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 26 1.4 - 2.2 C 1.79 0.21 2.6 - 3.9 P 3.24 0.26 0 - 0.4 C 0.04 0.05 1.9 - 2.9 P 2.4 0.2 1.6 - 2.4 P 2.03 0.17 8Initial Grouping by ReagentAbbott Architect 27 1.5 - 2.3 C 1.9 0.18 2.7 - 4.0 P 3.37 0.27 0 - 0.5 C 0.11 0.04 2.0 - 3.0 P 2.5 0.21 1.7 - 2.6 P 2.16 0.19 26Alfa Wassermann 28 1.7 - 2.6 P 2.16 0.18 3.1 - 4.7 P 3.93 0.28 0 - 0.5 C 0.13 0.06 2.3 - 3.5 P 2.89 0.23 2.0 - 2.9 P 2.44 0.22 29Beckman Coulter 29 1.9 - 2.8 P 2.34 0.18 3.2 - 4.9 P 4.05 0.23 0 - 0.6 C 0.19 0.1 2.4 - 3.7 P 3.05 0.23 2.1 - 3.1 P 2.57 0.2 35Beckman Olympus 30 1.9 - 2.8 P 2.34 0.16 3.4 - 5.1 P 4.22 0.23 0 - 0.5 C 0.11 0.03 2.4 - 3.6 P 3.0 0.17 2.2 - 3.3 P 2.73 0.17 55Carolina 31 1.8 - 2.6 P 2.19 0.21 3.1 - 4.7 P 3.88 0.38 0 - 0.6 C 0.19 0.12 2.3 - 3.5 P 2.93 0.33 2.0 - 3.0 P 2.48 0.26 19Horiba ABX 32 1.6 - 2.4 C 1.99 0.14 2.9 - 4.4 P 3.66 0.18 0 - 0.5 C 0.07 0.05 2.1 - 3.2 P 2.63 0.19 1.8 - 2.7 P 2.26 0.16 9Ortho Vitros 33 1.7 - 2.5 P 2.11 0.19 3.2 - 4.8 P 3.97 0.3 0 - 0.5 C 0.1 0.02 2.3 - 3.5 P 2.89 0.24 1.9 - 2.9 P 2.4 0.2 52Pointe Scientific 34 1.5 - 2.3 C 1.94 0.28 2.8 - 4.2 P 3.54 0.42 0 - 0.5 C 0.1 0.08 2.1 - 3.1 P 2.58 0.23 1.7 - 2.6 P 2.17 0.33 14Roche Cobas 35 1.4 - 2.2 C 1.77 0.14 2.6 - 3.9 P 3.24 0.26 0 - 0.5 C 0.1 0.09 1.9 - 2.8 P 2.35 0.2 1.6 - 2.4 P 2.03 0.17 24Roche Cobas BILTS 36 1.3 - 2.1 C 1.73 0.1 2.6 - 3.9 P 3.23 0.19 0 - 0.5 C 0.13 0.1 1.9 - 2.8 P 2.32 0.16 1.6 - 2.4 C 1.99 0.13 23SDI Biomed 37 2.1 - 3.1 P 2.6 0.12 3.8 - 5.8 P 4.8 0.3 0 - 0.5 C 0.09 0.03 2.8 - 4.2 P 3.54 0.16 2.3 - 3.5 P 2.9 0.13 13Sekisui 38 1.4 - 2.2 C 1.8 0.18 2.8 - 4.2 P 3.46 0.45 0 - 0.5 C 0.12 0.13 2.0 - 3.1 P 2.56 0.34 1.7 - 2.6 P 2.17 0.37 11Siemens Advia 39 1.6 - 2.4 P 2.03 0.16 3.0 - 4.4 P 3.7 0.26 0 - 0.5 C 0.05 0.05 2.2 - 3.2 P 2.7 0.19 1.8 - 2.7 P 2.28 0.17 6Siemens Dimension 40 1.5 - 2.3 C 1.9 0.16 2.8 - 4.2 P 3.51 0.24 0 - 0.5 C 0.07 0.05 2.0 - 3.1 P 2.56 0.17 1.7 - 2.6 P 2.15 0.16 117Siemens caffeine 41 1.7 - 2.5 P 2.08 0.17 3.1 - 4.6 P 3.82 0.19 0 - 0.5 C 0.06 0.05 2.2 - 3.3 P 2.78 0.15 1.9 - 2.8 P 2.36 0.17 5Synermed 42 1.9 - 2.8 P 2.34 0.21 3.3 - 5.0 P 4.18 0.42 0 - 0.5 C 0.08 0.04 2.3 - 3.4 P 2.86 0.21 2.1 - 3.2 P 2.66 0.16 5Unistat 43 1.4 - 2.2 C 1.76 0.16 2.8 - 4.2 P 3.52 0.25 0 - 0.4 C 0.0 0.0 2.0 - 3.0 P 2.46 0.17 1.4 - 2.2 C 1.8 0.14 5Vital Diagnostic 44 1.5 - 2.3 C 1.94 0.05 3.1 - 4.6 P 3.84 0.41 0 - 0.6 C 0.2 0.13 2.2 - 3.3 P 2.78 0.23 1.9 - 2.9 P 2.38 0.29 5Vital Diagnostics Envoy 45 1.4 - 2.2 C 1.79 0.21 2.6 - 3.9 P 3.24 0.26 0 - 0.4 C 0.04 0.05 1.9 - 2.9 P 2.4 0.2 1.6 - 2.4 P 2.03 0.17 8Initial Grouping by Sensitivity or PrincipleDiazo/DMSO 49 1.6 - 2.4 P 2.03 0.25 3.0 - 4.5 P 3.72 0.41 0 - 0.5 C 0.11 0.08 2.2 - 3.3 P 2.74 0.29 1.8 - 2.8 P 2.31 0.29 58Diazo/caffeine-benzoate 50 1.6 - 2.4 C 1.99 0.25 2.9 - 4.4 P 3.63 0.34 0 - 0.5 C 0.1 0.09 2.1 - 3.2 P 2.67 0.28 1.8 - 2.7 P 2.25 0.26 168Diazo/surfactant 46 1.5 - 2.3 C 1.92 0.37 2.9 - 4.3 P 3.6 0.7 0 - 0.5 C 0.11 0.09 2.1 - 3.1 P 2.6 0.54 1.8 - 2.6 P 2.2 0.41 65Diazonium ion 48 1.7 - 2.6 P 2.15 0.25 3.1 - 4.7 P 3.9 0.46 0 - 0.5 C 0.11 0.06 2.3 - 3.4 P 2.85 0.29 2.0 - 3.0 P 2.47 0.29 165Direct spectrophotometric 52 1.4 - 2.2 C 1.76 0.16 2.8 - 4.2 P 3.52 0.25 0 - 0.4 C 0.0 0.0 2.0 - 3.0 P 2.46 0.17 1.4 - 2.2 C 1.8 0.14 5Oxidation 47 1.7 - 2.6 P 2.18 0.22 3.1 - 4.7 P 3.9 0.39 0 - 0.5 C 0.08 0.07 2.2 - 3.3 P 2.78 0.2 2.0 - 3.0 P 2.46 0.24 13Other 51 1.7 - 2.6 P 2.17 0.35 3.2 - 4.7 P 3.95 0.63 0 - 0.5 C 0.09 0.05 2.3 - 3.4 P 2.87 0.33 2.0 - 3.0 P 2.52 0.39 10Total PopulationWhole Population 53 1.7 - 2.5 P 2.07 0.55 3.0 - 4.5 P 3.74 0.49 0 - 0.5 C 0.14 0.54 2.2 - 3.3 P 2.74 0.43 1.9 - 2.8 P 2.33 0.36 484

CalciumInitial Grouping by Reagent and InstrumentAbbott & Abbott Architect c, ci, i 1 9.0 - 11.0 C 9.98 0.13 11.4 - 13.4 C 12.41 0.15 7.1 - 9.1 C 8.08 0.12 8.6 - 10.6 C 9.59 0.23 10.7 - 12.7 C 11.72 0.14 26Alfa Wassermann & Alfa Wasser Axcel/Alera 2 8.8 - 10.8 C 9.77 0.27 11.1 - 13.1 C 12.13 0.35 7.2 - 9.2 C 8.19 0.24 8.5 - 10.5 C 9.47 0.21 10.7 - 12.7 C 11.71 0.23 29Beckman Coulter & Beck Coult Unicel DXC 3 8.8 - 10.8 C 9.79 0.14 11.2 - 13.2 C 12.15 0.17 7.0 - 9.0 C 7.97 0.09 8.4 - 10.4 C 9.37 0.16 10.6 - 12.6 C 11.59 0.14 10Beckman Coulter & Beck Olym AU 400/600/5400 4 9.0 - 11.0 C 9.95 0.13 11.2 - 13.2 C 12.2 0.16 7.3 - 9.3 C 8.25 0.1 8.6 - 10.6 C 9.57 0.14 10.6 - 12.6 C 11.6 0.14 6Beckman Coulter & Beckman AU 480 5 8.9 - 10.9 C 9.9 0.12 11.2 - 13.2 C 12.23 0.08 7.1 - 9.1 C 8.08 0.04 8.5 - 10.5 C 9.45 0.09 10.6 - 12.6 C 11.63 0.16 4Beckman ISE & Beck Coult Unicel DXC 6 8.9 - 10.9 C 9.87 0.18 11.3 - 13.3 C 12.26 0.18 7.0 - 9.0 C 8.03 0.14 8.5 - 10.5 C 9.45 0.15 10.6 - 12.6 C 11.62 0.15 16Beckman Olympus Arsenazo & Beck Olym AU400/600/5400 7 8.9 - 10.9 C 9.87 0.21 11.2 - 13.2 C 12.18 0.27 7.2 - 9.2 C 8.17 0.16 8.5 - 10.5 C 9.5 0.17 10.6 - 12.6 C 11.58 0.28 30

Beckman Olympus Arsenazo & Beckman AU 480 8 8.9 - 10.9 C 9.93 0.16 11.3 - 13.3 C 12.26 0.18 7.2 - 9.2 C 8.18 0.15 8.5 - 10.5 C 9.54 0.17 10.7 - 12.7 C 11.66 0.16 13Carolina & Beckman Synchron CX3/7/9/L 9 9.1 - 11.1 C 10.1 0.47 11.4 - 13.4 C 12.4 0.51 7.1 - 9.1 C 8.11 0.45 8.7 - 10.7 C 9.65 0.34 10.8 - 12.8 C 11.83 0.47 8Horiba ABX & Horiba ABX 400 10 8.9 - 10.9 C 9.9 0.29 11.9 - 13.9 C 12.92 0.39 6.8 - 8.8 C 7.76 0.21 8.4 - 10.4 C 9.39 0.24 11.2 - 13.2 C 12.19 0.37 9Medica Easy RA & Medica Easy RA 11 9.0 - 11.0 C 10.0 0.36 11.4 - 13.4 C 12.43 0.26 7.0 - 9.0 C 8.0 0.19 8.5 - 10.5 C 9.46 0.16 10.7 - 12.7 C 11.67 0.28 7Ortho Vitros & Ortho Vitros 3600, 5600 12 9.1 - 11.1 C 10.13 0.16 11.7 - 13.7 C 12.74 0.27 6.9 - 8.9 C 7.9 0.19 8.6 - 10.6 C 9.62 0.25 11.2 - 13.2 C 12.17 0.25 9Ortho Vitros & Ortho Vitros 5,1 FS 13 9.1 - 11.1 C 10.07 0.09 11.8 - 13.8 C 12.77 0.14 6.9 - 8.9 C 7.87 0.09 8.6 - 10.6 C 9.55 0.11 11.1 - 13.1 C 12.12 0.18 6Ortho Vitros & Ortho Vitros not DT or ECi 14 9.1 - 11.1 C 10.06 0.21 11.8 - 13.8 C 12.79 0.26 6.9 - 8.9 C 7.87 0.16 8.6 - 10.6 C 9.6 0.19 11.2 - 13.2 C 12.18 0.26 33Roche Cobas & Roche Cobas Integra 15 9.1 - 11.1 C 10.12 0.22 11.8 - 13.8 C 12.79 0.26 7.2 - 9.2 C 8.2 0.15 8.6 - 10.6 C 9.63 0.21 11.1 - 13.1 C 12.1 0.23 13Roche Cobas & Roche e/c, 1XX, X000, Elec series 16 9.1 - 11.1 C 10.11 0.21 11.7 - 13.7 C 12.71 0.28 7.1 - 9.1 C 8.14 0.2 8.7 - 10.7 C 9.67 0.22 11.1 - 13.1 C 12.08 0.26 28SDI Biomed & SDI CA-240, 480 17 8.9 - 10.9 C 9.89 0.38 11.6 - 13.6 C 12.6 0.47 6.6 - 8.6 C 7.61 0.34 8.6 - 10.6 C 9.58 0.39 11.1 - 13.1 C 12.13 0.49 10Sekisui & Beck Olym AU 400/600/5400 18 9.0 - 11.0 C 10.03 0.43 11.6 - 13.6 C 12.57 0.51 7.0 - 9.0 C 7.95 0.26 8.5 - 10.5 C 9.52 0.32 10.8 - 12.8 C 11.77 0.35 6Siemens OCPC & Siemens Dim RxL, RxL Max 19 8.6 - 10.6 C 9.56 0.24 11.1 - 13.1 C 12.13 0.29 6.7 - 8.7 C 7.72 0.18 8.1 - 10.1 C 9.11 0.24 10.6 - 12.6 C 11.55 0.35 10Siemens OCPC & Siemens Dimension 20 8.7 - 10.7 C 9.73 0.19 11.3 - 13.3 C 12.29 0.22 6.7 - 8.7 C 7.71 0.14 8.2 - 10.2 C 9.16 0.19 10.7 - 12.7 C 11.7 0.22 8Siemens OCPC & Siemens Dimension EXL 21 8.6 - 10.6 C 9.6 0.17 11.2 - 13.2 C 12.17 0.22 6.7 - 8.7 C 7.71 0.18 8.1 - 10.1 C 9.09 0.17 10.6 - 12.6 C 11.58 0.23 58Siemens OCPC & Siemens Dimension Xpand 22 8.6 - 10.6 C 9.63 0.22 11.2 - 13.2 C 12.19 0.24 6.7 - 8.7 C 7.72 0.22 8.1 - 10.1 C 9.12 0.21 10.6 - 12.6 C 11.61 0.23 38Siemens arsenazo & Siemens Advia 23 9.1 - 11.1 C 10.05 0.26 11.3 - 13.3 C 12.32 0.17 7.1 - 9.1 C 8.12 0.12 8.6 - 10.6 C 9.58 0.11 10.7 - 12.7 C 11.66 0.17 6Synermed & Synermed IR500 24 8.7 - 10.7 C 9.65 0.58 11.0 - 13.0 C 12.02 0.95 7.5 - 9.5 C 8.48 0.35 8.0 - 10.0 C 9.02 0.58 10.2 - 12.2 C 11.18 0.42 4Vital Diagnostic & Vital Diagnostic Eon 25 8.4 - 10.4 C 9.4 0.41 11.0 - 13.0 C 12.03 0.22 7.0 - 9.0 C 7.98 0.22 8.3 - 10.3 C 9.25 0.45 10.2 - 12.2 C 11.23 0.5 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 26 8.6 - 10.6 C 9.62 0.15 10.9 - 12.9 C 11.89 0.3 7.0 - 9.0 C 8.04 0.13 8.3 - 10.3 C 9.29 0.18 10.3 - 12.3 C 11.28 0.2 9Initial Grouping by ReagentAbbott 27 9.0 - 11.0 C 9.98 0.13 11.4 - 13.4 C 12.41 0.15 7.1 - 9.1 C 8.08 0.12 8.6 - 10.6 C 9.59 0.23 10.7 - 12.7 C 11.72 0.14 26Alfa Wassermann 28 8.8 - 10.8 C 9.76 0.27 11.2 - 13.2 C 12.15 0.34 7.2 - 9.2 C 8.2 0.23 8.5 - 10.5 C 9.47 0.2 10.8 - 12.8 C 11.75 0.29 32Beckman Coulter 29 8.9 - 10.9 C 9.86 0.15 11.2 - 13.2 C 12.18 0.16 7.1 - 9.1 C 8.07 0.15 8.4 - 10.4 C 9.44 0.16 10.6 - 12.6 C 11.6 0.15 21Beckman ISE 30 8.9 - 10.9 C 9.89 0.2 11.3 - 13.3 C 12.28 0.18 7.0 - 9.0 C 8.04 0.15 8.5 - 10.5 C 9.46 0.15 10.6 - 12.6 C 11.64 0.15 18Beckman Olympus Arsenazo 31 8.9 - 10.9 C 9.89 0.2 11.2 - 13.2 C 12.21 0.25 7.2 - 9.2 C 8.17 0.18 8.5 - 10.5 C 9.5 0.19 10.6 - 12.6 C 11.61 0.25 47Beckman Olympus OCPC 32 9.1 - 11.1 C 10.06 0.22 11.5 - 13.5 C 12.52 0.34 7.2 - 9.2 C 8.22 0.21 8.6 - 10.6 C 9.62 0.23 10.8 - 12.8 C 11.84 0.31 5Carolina 33 8.9 - 10.9 C 9.93 0.39 11.3 - 13.3 C 12.26 0.45 6.8 - 8.8 C 7.84 0.44 8.6 - 10.6 C 9.56 0.34 10.6 - 12.6 C 11.6 0.42 19Horiba ABX 34 8.9 - 10.9 C 9.9 0.29 11.9 - 13.9 C 12.92 0.39 6.8 - 8.8 C 7.76 0.21 8.4 - 10.4 C 9.39 0.24 11.2 - 13.2 C 12.19 0.37 9Medica Easy RA 35 9.0 - 11.0 C 10.0 0.36 11.4 - 13.4 C 12.43 0.26 7.0 - 9.0 C 8.0 0.19 8.5 - 10.5 C 9.46 0.16 10.7 - 12.7 C 11.67 0.28 7Ortho Vitros 36 9.1 - 11.1 C 10.08 0.19 11.8 - 13.8 C 12.79 0.25 6.9 - 8.9 C 7.88 0.16 8.6 - 10.6 C 9.61 0.2 11.2 - 13.2 C 12.18 0.25 52Pointe Sci Arsenazo 37 8.6 - 10.6 C 9.55 0.05 11.1 - 13.1 C 12.05 0.21 7.1 - 9.1 C 8.05 0.38 8.5 - 10.5 C 9.5 0.47 10.9 - 12.9 C 11.9 0.39 4Pointe Sci OCPC 38 8.7 - 10.7 C 9.69 0.41 11.4 - 13.4 C 12.36 0.51 6.9 - 8.9 C 7.89 0.33 8.1 - 10.1 C 9.12 0.37 10.8 - 12.8 C 11.81 0.55 10Roche Cobas 39 9.1 - 11.1 C 10.13 0.22 11.7 - 13.7 C 12.73 0.27 7.2 - 9.2 C 8.17 0.19 8.7 - 10.7 C 9.66 0.21 11.1 - 13.1 C 12.09 0.25 45SDI Biomed 40 8.8 - 10.8 C 9.84 0.37 11.7 - 13.7 C 12.65 0.44 6.6 - 8.6 C 7.62 0.32 8.6 - 10.6 C 9.55 0.36 11.1 - 13.1 C 12.06 0.5 12Sekisui 41 9.1 - 11.1 C 10.12 0.39 11.4 - 13.4 C 12.37 0.49 7.1 - 9.1 C 8.06 0.33 8.6 - 10.6 C 9.55 0.4 10.8 - 12.8 C 11.82 0.38 12Siemens OCPC 42 8.6 - 10.6 C 9.63 0.21 11.2 - 13.2 C 12.19 0.25 6.7 - 8.7 C 7.72 0.2 8.1 - 10.1 C 9.11 0.2 10.6 - 12.6 C 11.6 0.25 116Siemens arsenazo 43 9.0 - 11.0 C 9.95 0.28 11.3 - 13.3 C 12.29 0.16 7.0 - 9.0 C 8.01 0.21 8.5 - 10.5 C 9.46 0.23 10.6 - 12.6 C 11.64 0.16 8Synermed 44 8.7 - 10.7 C 9.66 0.52 11.0 - 13.0 C 11.96 0.86 7.4 - 9.4 C 8.4 0.35 8.1 - 10.1 C 9.1 0.54 10.3 - 12.3 C 11.26 0.41 5Vital Diagnostic 45 8.4 - 10.4 C 9.4 0.41 11.0 - 13.0 C 12.03 0.22 7.0 - 9.0 C 7.98 0.22 8.3 - 10.3 C 9.25 0.45 10.2 - 12.2 C 11.23 0.5 4Vital Diagnostics Envoy 46 8.6 - 10.6 C 9.62 0.15 10.9 - 12.9 C 11.89 0.3 7.0 - 9.0 C 8.04 0.13 8.3 - 10.3 C 9.29 0.18 10.3 - 12.3 C 11.28 0.2 9Initial Grouping by Sensitivity or PrincipleArsenazo-based 47 8.9 - 10.9 C 9.91 0.29 11.4 - 13.4 C 12.37 0.42 7.0 - 9.0 C 8.02 0.29 8.5 - 10.5 C 9.51 0.28 10.8 - 12.8 C 11.79 0.4 273OCPC (o-cresolphth complex) 49 8.8 - 10.8 C 9.77 0.32 11.3 - 13.3 C 12.34 0.36 6.9 - 8.9 C 7.86 0.29 8.3 - 10.3 C 9.27 0.33 10.7 - 12.7 C 11.74 0.36 184Other, electrochemical 48 8.9 - 10.9 C 9.89 0.2 11.3 - 13.3 C 12.28 0.18 7.0 - 9.0 C 8.04 0.15 8.5 - 10.5 C 9.46 0.15 10.6 - 12.6 C 11.64 0.15 18Other 50 8.8 - 10.8 C 9.77 0.42 11.2 - 13.2 C 12.2 0.57 7.0 - 9.0 C 8.02 0.5 8.3 - 10.3 C 9.31 0.42 10.6 - 12.6 C 11.64 0.56 11Total PopulationWhole Population 51 8.9 - 10.9 C 9.85 0.32 11.5 - 13.5 C 12.52 4.96 7.0 - 9.0 C 7.97 0.32 8.4 - 10.4 C 9.41 0.33 10.7 - 12.7 C 11.72 0.66 489

ChlorideInitial Grouping by Reagent and InstrumentAbbott & Abbott Architect c, ci, i 1 99 - 110 P 104.7 0.8 91 - 100 P 95.6 0.7 107 - 118 P 112.8 0.9 105 - 117 P 111.0 0.8 89 - 99 P 94.0 0.9 26Alfa Wassermann & Alfa Wasser Axcel/Alera 2 101 - 111 P 106.1 1.3 90 - 100 P 94.9 1.2 104 - 115 P 109.8 1.0 108 - 119 P 113.2 1.2 88 - 97 P 92.2 1.3 26

Beckman Coulter & Beck Coult Unicel DXC 3 98 - 108 P 103.0 0.9 90 - 99 P 94.6 1.3 106 - 118 P 112.1 1.8 104 - 115 P 109.9 0.9 88 - 98 P 92.9 1.1 26Beckman Coulter & Beck Olym AU 400/600/5400 4 99 - 110 P 104.5 2.7 90 - 99 P 94.3 2.9 106 - 117 P 111.8 3.6 104 - 115 P 109.8 1.5 88 - 97 P 92.5 2.7 4Beckman Coulter & Beckman AU 480 5 99 - 110 P 104.5 1.1 90 - 99 P 94.3 1.5 105 - 116 P 110.8 0.8 105 - 116 P 110.8 1.1 88 - 97 P 92.8 0.8 4Beckman Olympus & Beck Olym AU 400/600/5400 6 98 - 109 P 103.5 1.1 89 - 98 P 93.8 1.1 105 - 116 P 110.4 1.0 104 - 115 P 109.7 1.5 87 - 97 P 92.1 0.6 42Beckman Olympus & Beck Olympus AU 2700 7 99 - 109 P 104.0 1.2 89 - 98 P 93.5 0.5 105 - 116 P 110.8 0.8 104 - 115 P 109.8 1.3 87 - 96 P 91.8 0.8 4Beckman Olympus & Beckman AU 480 8 98 - 109 P 103.4 1.7 88 - 98 P 93.1 0.8 104 - 115 P 109.8 1.1 104 - 115 P 109.6 2.2 87 - 96 P 91.7 0.9 15Carolina & Beckman Synchron CX3/7/9/L 9 100 - 111 P 105.7 3.6 93 - 103 P 97.8 3.6 108 - 119 P 113.7 5.7 106 - 117 P 111.3 3.7 96 - 106 P 101.0 9.8 9Horiba ABX & Horiba ABX 400 10 100 - 110 P 104.9 2.2 89 - 99 P 94.1 1.5 98 - 108 P 103.1 1.4 108 - 120 P 114.1 3.3 86 - 95 P 90.9 1.9 9Medica Easy RA & Medica Easy RA 11 99 - 109 P 104.0 2.0 89 - 99 P 93.9 1.1 100 - 110 P 105.1 1.2 108 - 120 P 114.0 1.2 86 - 95 P 90.1 0.8 8Medica EasyLyte & Medica EasyLyte series 12 101 - 112 P 106.8 1.2 93 - 103 P 98.0 5.6 104 - 114 P 109.0 3.2 111 - 123 P 116.8 1.5 87 - 96 P 91.2 1.0 5Ortho Vitros & Ortho Vitros 3600, 5600 13 100 - 110 P 105.0 1.5 91 - 100 P 95.4 1.3 105 - 116 P 110.3 1.7 107 - 118 P 112.3 2.1 89 - 99 P 93.9 2.4 8Ortho Vitros & Ortho Vitros 5,1 FS 14 98 - 108 P 102.7 0.7 90 - 99 P 94.3 0.7 103 - 113 P 108.0 0.6 104 - 115 P 109.7 0.7 87 - 96 P 91.8 0.9 6Ortho Vitros & Ortho Vitros not DT or ECi 15 99 - 109 P 103.8 1.3 90 - 99 P 94.7 1.2 104 - 115 P 109.4 1.3 105 - 116 P 110.9 1.4 88 - 98 P 93.0 1.3 33Roche Cobas dil ISE & Roche Cobas Integra 16 99 - 109 P 103.8 1.1 88 - 97 P 92.8 1.2 104 - 115 P 109.2 1.5 105 - 116 P 110.2 1.2 86 - 95 P 90.8 1.1 13Roche Cobas dil ISE & Roche e/c, 1XX, X000, Elecseries 17 96 - 106 P 101.0 1.7 85 - 94 P 89.3 1.8 101 - 112 P 106.3 1.2 103 - 114 P 108.9 1.4 83 - 92 P 87.6 2.0 22

Roche Cobas und ISE & Roche e/c, 1XX, X000, Elecseries 18 98 - 108 P 103.0 1.7 86 - 96 P 91.0 2.3 103 - 114 P 108.5 2.2 105 - 116 P 110.0 1.7 85 - 94 P 89.3 2.5 6

SDI Biomed & SDI CA-240, 480 19 97 - 107 P 102.1 1.4 89 - 98 P 93.6 1.5 98 - 109 P 103.4 2.1 103 - 114 P 108.2 1.6 86 - 95 P 90.6 1.9 10Siemens Advia & Siemens Advia 20 101 - 112 P 106.4 1.5 91 - 101 P 96.1 1.2 106 - 117 P 111.8 1.0 107 - 119 P 113.0 1.7 89 - 98 P 93.5 1.1 8Siemens QuickLYTE IMT & Siemens Dim RxL, RxLMax 21 98 - 109 P 103.4 1.9 86 - 95 P 90.5 1.8 105 - 116 P 110.8 3.6 104 - 115 P 109.1 2.3 85 - 94 P 89.7 2.3 10

Siemens QuickLYTE IMT & Siemens Dimension 22 99 - 109 P 103.8 1.5 86 - 95 P 90.8 1.2 107 - 118 P 112.5 3.4 104 - 115 P 109.5 1.3 86 - 95 P 90.0 1.0 8Siemens QuickLYTE IMT & Siemens Dimension EXL 23 99 - 109 P 103.8 0.8 87 - 96 P 91.6 0.8 107 - 118 P 112.4 2.8 105 - 116 P 110.1 0.8 86 - 95 P 90.5 0.7 60Siemens QuickLYTE IMT & Siemens DimensionXpand 24 99 - 109 P 104.0 1.0 87 - 96 P 91.3 1.2 108 - 119 P 113.7 3.7 105 - 115 P 110.0 1.2 86 - 95 P 90.5 1.0 43

Vital Diagnostic Eon & Vital Diagnostic Eon 25 98 - 108 P 103.3 3.3 89 - 99 P 94.0 4.1 98 - 108 P 102.8 2.5 106 - 117 P 111.5 3.2 84 - 93 P 88.7 1.7 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 26 100 - 110 P 105.1 2.0 91 - 100 P 95.3 1.4 108 - 119 P 113.2 4.0 108 - 119 P 113.2 2.6 89 - 99 P 94.0 2.7 9Initial Grouping by ReagentAVL 27 98 - 109 P 103.5 1.8 89 - 98 P 93.8 1.1 102 - 112 P 107.0 1.9 106 - 117 P 111.3 1.5 86 - 96 P 91.0 1.2 4Abbott 28 99 - 110 P 104.7 0.8 91 - 100 P 95.6 0.7 107 - 118 P 112.8 0.9 105 - 117 P 111.0 0.8 89 - 99 P 94.0 0.9 26Alfa Wassermann 29 101 - 111 P 106.1 1.3 90 - 100 P 94.8 1.2 104 - 115 P 109.7 1.2 107 - 119 P 113.1 1.2 88 - 97 P 92.2 1.2 29Beckman Coulter 30 98 - 109 P 103.4 1.4 90 - 99 P 94.6 1.6 106 - 117 P 111.9 2.0 105 - 116 P 110.1 1.0 88 - 98 P 92.9 1.4 36Beckman Olympus 31 98 - 109 P 103.5 1.3 89 - 98 P 93.6 1.0 105 - 116 P 110.3 1.0 104 - 115 P 109.7 1.7 87 - 97 P 92.0 0.8 63Carolina 32 100 - 110 P 104.8 4.4 92 - 102 P 97.0 3.7 106 - 118 P 112.0 6.8 106 - 118 P 112.1 4.5 94 - 103 P 98.5 8.8 15Diamond Diagnostics 33 99 - 110 P 104.4 2.0 91 - 100 P 95.4 3.5 106 - 117 P 111.8 3.2 105 - 116 P 110.8 3.1 89 - 98 P 93.4 3.9 5Horiba ABX 34 100 - 110 P 104.9 2.2 89 - 99 P 94.1 1.5 98 - 108 P 103.1 1.4 108 - 120 P 114.1 3.3 86 - 95 P 90.9 1.9 9Medica Easy RA 35 99 - 109 P 104.0 2.0 89 - 99 P 93.9 1.1 100 - 110 P 105.1 1.2 108 - 120 P 114.0 1.2 86 - 95 P 90.1 0.8 8Medica EasyLyte 36 101 - 112 P 106.2 1.9 92 - 101 P 96.6 4.5 103 - 114 P 108.1 2.8 110 - 121 P 115.3 2.6 87 - 96 P 91.6 1.1 9Ortho Vitros 37 99 - 109 P 103.9 1.4 90 - 100 P 94.9 1.2 104 - 115 P 109.4 1.5 106 - 117 P 111.1 1.6 88 - 98 P 93.1 1.6 51Pointe Scientific 38 98 - 108 P 102.8 5.8 89 - 98 P 93.8 4.9 102 - 112 P 107.0 5.2 104 - 115 P 109.4 6.5 86 - 96 P 91.0 3.3 5Roche Cobas dil ISE 39 97 - 107 P 101.9 2.0 86 - 95 P 90.3 2.5 102 - 113 P 107.2 1.9 104 - 115 P 109.2 1.5 84 - 93 P 88.6 2.4 38Roche Cobas und ISE 40 97 - 108 P 102.5 1.9 87 - 96 P 91.5 2.0 102 - 112 P 106.9 3.5 104 - 115 P 109.5 1.7 85 - 94 P 89.4 2.2 11SDI Biomed 41 97 - 107 P 102.3 1.4 89 - 98 P 93.4 1.8 98 - 108 P 103.3 2.0 103 - 114 P 108.3 1.7 86 - 95 P 90.2 2.1 12Siemens Advia 42 101 - 112 P 106.4 1.5 91 - 101 P 96.1 1.2 106 - 117 P 111.8 1.0 107 - 119 P 113.0 1.7 89 - 98 P 93.5 1.1 8Siemens QuickLYTE IMT 43 99 - 109 P 103.8 1.1 87 - 96 P 91.3 1.1 107 - 118 P 112.7 3.4 104 - 115 P 109.9 1.2 86 - 95 P 90.4 1.1 121Vital Diagnostic Eon 44 98 - 108 P 103.3 3.3 89 - 99 P 94.0 4.1 98 - 108 P 102.8 2.5 106 - 117 P 111.5 3.2 84 - 93 P 88.7 1.7 4Vital Diagnostics Envoy 45 100 - 110 P 105.1 2.0 91 - 100 P 95.3 1.4 108 - 119 P 113.2 4.0 108 - 119 P 113.2 2.6 89 - 99 P 94.0 2.7 9Initial Grouping by Sensitivity or PrincipleColorimetric 48 98 - 108 P 102.7 3.3 89 - 98 P 93.8 3.1 100 - 111 P 105.4 4.7 103 - 114 P 108.7 3.6 86 - 95 P 90.7 2.6 19Diluted ISE 46 99 - 109 P 103.7 1.9 88 - 98 P 92.9 2.6 106 - 117 P 111.3 3.5 105 - 116 P 110.2 2.0 87 - 96 P 91.6 3.2 329Other 49 101 - 111 P 105.9 5.5 92 - 102 P 97.3 8.2 106 - 117 P 111.6 5.5 107 - 119 P 113.0 6.6 88 - 98 P 93.0 4.2 8Undiluted ISE 47 99 - 110 P 104.5 2.0 90 - 99 P 94.6 2.2 103 - 114 P 108.5 2.8 106 - 118 P 112.0 2.5 87 - 97 P 92.1 2.1 128Total PopulationWhole Population 50 99 - 109 P 104.0 2.3 89 - 98 P 93.5 2.9 105 - 116 P 110.2 4.8 105 - 116 P 110.9 5.5 87 - 96 P 91.9 3.8 484

Cholesterol, TotalInitial Grouping by Reagent and InstrumentAbbott & Abbott Architect c, ci, i 1 163 - 199 P 181.1 1.8 174 - 213 P 193.8 2.1 42 - 52 P 47.2 1.1 201 - 246 P 223.6 2.0 131 - 160 P 145.4 1.6 26Alfa Wassermann & Alfa Wasser Axcel/Alera 2 161 - 196 P 178.4 4.0 173 - 212 P 192.6 5.2 42 - 52 P 46.9 1.4 198 - 242 P 219.9 5.0 130 - 159 P 144.1 3.2 25Beckman Coulter & Beck Coult Unicel DXC 3 164 - 201 P 182.7 3.2 177 - 216 P 196.8 3.5 40 - 48 P 43.9 0.8 204 - 250 P 227.0 3.3 131 - 161 P 146.1 2.2 23Beckman Olympus & Beck Olym AU 400/600/5400 4 156 - 191 P 173.2 5.0 167 - 205 P 186.0 5.9 38 - 46 P 42.0 1.6 193 - 236 P 214.9 5.9 125 - 152 P 138.6 4.5 31Beckman Olympus & Beck Olympus AU 2700 5 156 - 190 P 172.8 1.5 166 - 203 P 184.8 2.2 38 - 46 P 41.8 0.4 191 - 233 P 211.8 3.0 124 - 152 P 137.8 2.5 4

Beckman Olympus & Beckman AU 480 6 157 - 191 P 174.0 4.5 168 - 205 P 186.2 4.9 38 - 47 P 42.4 1.3 193 - 235 P 213.9 5.3 126 - 153 P 139.5 3.8 13Carolina & Beckman Synchron CX3/7/9/L 7 173 - 212 P 192.5 5.8 182 - 223 P 202.5 8.9 45 - 55 P 50.0 3.7 205 - 250 P 227.6 8.4 143 - 175 P 158.9 8.1 8Horiba ABX & Horiba ABX 400 8 159 - 194 P 176.8 5.3 170 - 208 P 188.8 4.6 41 - 50 P 45.2 1.2 195 - 239 P 217.0 6.7 129 - 158 P 143.3 5.2 6Ortho Vitros & Ortho Vitros 3600, 5600 9 158 - 194 P 176.0 4.2 177 - 216 P 196.6 4.2 45 - 55 P 50.0 0.0 202 - 247 P 224.6 7.0 127 - 155 P 140.9 3.1 8Ortho Vitros & Ortho Vitros 5,1 FS 10 159 - 194 P 176.5 2.7 174 - 213 P 193.3 4.4 45 - 55 P 50.0 0.0 198 - 242 P 220.0 4.2 124 - 152 P 138.3 2.7 6Ortho Vitros & Ortho Vitros not DT or ECi 11 155 - 190 P 172.4 7.4 174 - 213 P 193.5 6.4 42 - 51 P 46.6 2.7 195 - 239 P 217.2 6.6 126 - 154 P 139.6 4.3 27Pointe Scientific & Other spectrophotometers 12 161 - 197 P 178.7 5.8 171 - 209 P 190.2 8.3 44 - 54 P 49.2 1.3 198 - 242 P 219.8 8.3 131 - 160 P 145.7 4.4 6Pointe Scientific & Pointe Sci 180/Sterl 2000 13 165 - 201 P 182.8 2.7 177 - 217 P 197.2 5.9 43 - 52 P 47.3 2.6 200 - 244 P 222.1 4.6 133 - 163 P 147.9 6.5 10Roche Cobas & Roche Cobas Integra 14 159 - 195 P 177.0 3.3 171 - 209 P 190.2 3.4 40 - 49 P 44.3 0.9 197 - 241 P 218.9 5.0 128 - 157 P 142.6 2.9 12Roche Cobas & Roche e/c, 1XX, X000, Elec series 15 162 - 198 P 180.2 3.0 174 - 213 P 193.7 3.6 42 - 51 P 46.8 1.9 200 - 244 P 222.1 4.8 131 - 160 P 145.6 2.4 29SDI Biomed & SDI CA-240, 480 16 168 - 206 P 187.1 8.4 178 - 218 P 198.1 7.7 43 - 52 P 47.6 3.6 204 - 249 P 226.3 11.8 137 - 168 P 152.5 3.9 8Sekisui & Beck Olym AU 400/600/5400 17 156 - 191 P 173.5 9.1 171 - 208 P 189.5 10.1 39 - 47 P 43.0 1.9 197 - 241 P 219.3 15.8 125 - 153 P 139.3 4.3 4Sekisui & Roche Cobas Mira series 18 166 - 203 P 184.4 5.1 178 - 218 P 197.8 1.2 40 - 49 P 44.8 1.0 206 - 251 P 228.6 3.6 134 - 164 P 149.0 2.8 5Siemens Advia & Siemens Advia 19 162 - 197 P 179.5 3.5 174 - 213 P 193.8 3.6 41 - 50 P 45.8 3.6 198 - 242 P 220.3 4.3 131 - 160 P 145.3 3.7 4Siemens Dimension & Siemens Advia 20 161 - 197 P 179.0 7.3 178 - 217 P 197.3 7.3 41 - 50 P 45.5 2.1 201 - 245 P 222.8 8.8 132 - 162 P 147.0 5.0 4Siemens Dimension & Siemens Dim RxL, RxL Max 21 159 - 195 P 177.1 3.8 171 - 208 P 189.5 3.4 42 - 51 P 46.6 3.1 197 - 240 P 218.5 4.8 128 - 156 P 141.8 3.4 10Siemens Dimension & Siemens Dimension 22 161 - 197 P 179.1 5.7 173 - 212 P 192.4 5.6 43 - 52 P 47.4 3.0 200 - 244 P 221.7 5.0 129 - 157 P 143.1 3.4 7Siemens Dimension & Siemens Dimension EXL 23 159 - 194 P 176.4 3.3 169 - 207 P 187.8 3.7 43 - 52 P 47.5 2.7 195 - 239 P 217.0 3.6 126 - 154 P 140.0 3.5 58Siemens Dimension & Siemens Dimension Xpand 24 157 - 192 P 174.7 3.9 168 - 205 P 186.4 3.9 40 - 50 P 45.0 3.5 194 - 237 P 215.1 4.6 125 - 153 P 139.0 3.4 41Stering Diagnostic & Other spectrophotometers 25 160 - 196 P 178.1 3.9 173 - 211 P 191.7 4.2 42 - 51 P 46.7 3.9 198 - 241 P 219.5 7.9 129 - 158 P 143.8 4.7 11Stering Diagnostic & Pointe Sci 180/Sterl 2000 26 154 - 188 P 170.8 5.9 166 - 203 P 184.8 3.4 40 - 49 P 44.2 2.3 191 - 233 P 211.8 1.1 126 - 153 P 139.5 2.4 6Synermed & Synermed IR500 27 177 - 217 P 197.0 3.9 190 - 232 P 211.0 4.6 38 - 46 P 42.0 2.9 217 - 266 P 241.5 3.6 143 - 175 P 159.3 3.0 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 28 166 - 202 P 184.0 5.2 176 - 216 P 196.0 6.2 42 - 51 P 46.2 1.5 205 - 250 P 227.3 4.4 134 - 163 P 148.5 4.5 9Initial Grouping by ReagentAbbott 29 163 - 199 P 181.1 1.8 174 - 213 P 193.8 2.1 42 - 52 P 47.2 1.1 201 - 246 P 223.6 2.0 131 - 160 P 145.4 1.6 26Alfa Wassermann 30 160 - 196 P 177.9 4.3 173 - 212 P 192.3 5.1 42 - 51 P 46.8 1.3 198 - 242 P 219.8 4.9 130 - 158 P 144.0 3.1 27Beckman Coulter 31 163 - 200 P 181.5 4.3 175 - 214 P 194.8 6.4 39 - 48 P 43.6 1.3 203 - 248 P 225.4 6.4 131 - 160 P 145.7 3.4 32Beckman Olympus 32 156 - 191 P 173.5 4.7 168 - 205 P 186.2 5.5 38 - 46 P 42.1 1.4 193 - 236 P 214.6 5.6 125 - 153 P 138.9 4.1 50Carolina 33 171 - 209 P 190.0 8.5 181 - 222 P 201.6 9.8 45 - 55 P 49.8 3.8 205 - 250 P 227.3 9.9 140 - 171 P 155.1 7.4 19Horiba ABX 34 159 - 194 P 176.8 5.3 170 - 208 P 188.8 4.6 41 - 50 P 45.2 1.2 195 - 239 P 217.0 6.7 129 - 158 P 143.3 5.2 6Ortho Vitros 35 157 - 191 P 173.9 6.4 175 - 214 P 194.3 5.7 43 - 52 P 47.7 2.7 197 - 241 P 219.3 6.9 126 - 154 P 139.8 3.9 44Pointe Scientific 36 162 - 198 P 180.0 6.5 175 - 214 P 194.2 8.1 42 - 51 P 46.7 3.1 199 - 243 P 221.3 7.8 132 - 161 P 146.2 6.4 27Roche Cobas 37 161 - 197 P 179.4 3.4 174 - 212 P 192.8 3.8 41 - 51 P 46.0 2.0 199 - 244 P 221.4 5.0 130 - 159 P 144.8 2.9 45SDI Biomed 38 167 - 204 P 185.8 9.5 179 - 219 P 199.0 7.2 43 - 52 P 47.6 3.2 205 - 251 P 228.2 11.4 138 - 169 P 153.6 4.2 10Sekisui 39 164 - 200 P 182.0 11.2 176 - 215 P 195.9 10.2 40 - 49 P 44.6 2.4 204 - 250 P 227.2 13.4 132 - 161 P 146.3 7.8 12Siemens Advia 40 162 - 197 P 179.5 3.5 174 - 213 P 193.8 3.6 41 - 50 P 45.8 3.6 198 - 242 P 220.3 4.3 131 - 160 P 145.3 3.7 4Siemens Dimension 41 158 - 194 P 176.1 4.2 169 - 207 P 188.1 4.6 42 - 51 P 46.6 3.2 195 - 239 P 216.9 4.9 126 - 154 P 140.2 3.9 120Stering Diagnostic 42 158 - 193 P 175.9 5.9 171 - 209 P 190.1 5.9 41 - 50 P 45.9 3.6 196 - 240 P 218.2 9.2 128 - 157 P 142.6 4.6 18Synermed 43 177 - 216 P 196.6 3.6 190 - 232 P 211.0 4.1 37 - 45 P 40.6 3.8 218 - 266 P 241.8 3.2 142 - 174 P 158.2 3.4 5Vital Diagnostic 44 161 - 197 P 179.2 6.4 174 - 213 P 193.2 5.5 42 - 52 P 47.0 2.3 197 - 241 P 218.8 13.8 131 - 161 P 146.0 5.8 6Vital Diagnostics Envoy 45 166 - 202 P 184.0 5.2 176 - 216 P 196.0 6.2 42 - 51 P 46.2 1.5 205 - 250 P 227.3 4.4 134 - 163 P 148.5 4.5 9Initial Grouping by Sensitivity or PrincipleAll enzymatic cholesterol 46 160 - 196 P 178.3 6.9 173 - 211 P 191.9 7.3 41 - 50 P 45.9 3.3 198 - 242 P 220.2 8.0 129 - 158 P 143.4 6.1 475Other 47 158 - 193 P 175.3 14.5 148 - 181 P 164.7 51.4 44 - 54 P 49.2 13.1 175 - 214 P 194.7 47.9 124 - 152 P 138.3 8.6 7Total PopulationWhole Population 48 160 - 196 P 178.3 8.9 172 - 210 P 190.9 14.1 42 - 51 P 46.6 7.8 197 - 241 P 219.1 14.3 128 - 157 P 142.4 11.5 482

CreatinineInitial Grouping by Reagent and InstrumentAbbott Architect & Abbott Architect c, ci, i 1 2.6 - 3.5 P 3.08 0.05 4.7 - 6.4 P 5.58 0.1 0.4 - 1.0 C 0.71 0.04 2.9 - 3.9 P 3.42 0.05 3.5 - 4.8 P 4.14 0.09 26Alfa Wassermann & Alfa Wasser Axcel/Alera 2 2.4 - 3.3 P 2.86 0.16 4.3 - 5.8 P 5.02 0.28 0.5 - 1.1 C 0.78 0.08 2.6 - 3.5 P 3.02 0.17 3.3 - 4.5 P 3.94 0.18 29Beckman Coulter & Beck Coult Unicel DXC 3 2.5 - 3.3 P 2.89 0.09 4.5 - 6.0 P 5.24 0.16 0.3 - 0.9 C 0.57 0.04 2.7 - 3.6 P 3.15 0.14 3.4 - 4.6 P 3.99 0.15 17Beckman Coulter & Beck Olym AU 400/600/5400 4 2.5 - 3.4 P 2.98 0.08 4.5 - 6.1 P 5.3 0.1 0.4 - 1.0 C 0.68 0.04 2.8 - 3.7 P 3.25 0.05 3.1 - 4.3 P 3.7 0.52 4Beckman Olympus & Beck Olym AU 400/600/5400 5 2.5 - 3.4 P 2.94 0.08 4.5 - 6.1 P 5.28 0.11 0.4 - 1.0 C 0.67 0.08 2.7 - 3.7 P 3.22 0.08 3.4 - 4.6 P 4.01 0.12 36Beckman Olympus & Beck Olympus AU 2700 6 2.4 - 3.3 P 2.85 0.15 4.3 - 5.9 P 5.1 0.23 0.3 - 0.9 C 0.58 0.04 2.7 - 3.6 P 3.13 0.15 3.3 - 4.4 P 3.85 0.21 4Beckman Olympus & Beckman AU 480 7 2.5 - 3.3 P 2.91 0.09 4.5 - 6.1 P 5.27 0.15 0.3 - 0.9 C 0.63 0.05 2.7 - 3.7 P 3.22 0.1 3.4 - 4.6 P 4.0 0.11 15Beckman Synchron IDMS trace & Beck Coult UnicelDXC 8 2.5 - 3.3 P 2.89 0.11 4.3 - 5.8 P 5.0 0.64 0.3 - 0.9 C 0.57 0.07 2.7 - 3.6 P 3.14 0.13 3.4 - 4.6 P 3.97 0.12 9

Carolina & Beck Olym AU 400/600/5400 9 2.5 - 3.4 P 2.94 0.15 4.2 - 5.7 P 4.98 0.21 0.7 - 1.3 C 0.96 0.05 2.7 - 3.7 P 3.18 0.12 3.2 - 4.4 P 3.8 0.14 5Carolina & Beckman Synchron CX3/7/9/L 10 2.6 - 3.5 P 3.06 0.3 4.4 - 6.0 P 5.23 0.33 0.6 - 1.2 C 0.93 0.13 2.8 - 3.8 P 3.31 0.21 3.4 - 4.6 P 4.01 0.31 9Horiba ABX & Horiba ABX 400 11 2.4 - 3.3 P 2.84 0.13 4.3 - 5.9 P 5.09 0.11 0.4 - 1.0 C 0.71 0.1 2.7 - 3.6 P 3.12 0.09 3.3 - 4.5 P 3.89 0.12 9Medica & Medica Easy RA 12 2.5 - 3.4 P 2.99 0.06 4.6 - 6.2 P 5.4 0.12 0.3 - 0.9 C 0.6 0.0 2.8 - 3.7 P 3.26 0.09 3.5 - 4.7 P 4.06 0.12 7

Ortho Vitros 1-slide enz & Ortho Vitros not DT or ECi 13 2.5 - 3.4 P 2.95 0.09 4.5 - 6.1 P 5.33 1.63 0.4 - 1.0 C 0.67 0.04 2.6 - 3.5 P 3.05 0.12 3.5 - 4.7 P 4.09 0.14 12Roche Cobas & Roche Cobas Integra 14 2.4 - 3.2 P 2.82 0.13 4.3 - 5.9 P 5.09 0.13 0.4 - 1.0 C 0.65 0.05 2.6 - 3.5 P 3.03 0.1 3.3 - 4.5 P 3.93 0.1 11Roche Cobas & Roche e/c, 1XX, X000, Elec series 15 2.4 - 3.3 P 2.85 0.13 4.4 - 5.9 P 5.13 0.19 0.3 - 0.9 C 0.63 0.05 2.6 - 3.5 P 3.07 0.18 3.4 - 4.6 P 3.96 0.2 28SDI Biomed & SDI CA-240, 480 16 2.2 - 3.0 P 2.63 0.09 3.8 - 5.1 P 4.45 0.08 0.4 - 1.0 C 0.72 0.08 2.3 - 3.2 P 2.76 0.05 3.0 - 4.0 P 3.5 0.37 11Sekisui & Beck Olym AU 400/600/5400 17 2.5 - 3.4 P 2.96 0.2 4.3 - 5.8 P 5.02 0.25 0.6 - 1.2 C 0.86 0.05 2.7 - 3.7 P 3.18 0.17 3.3 - 4.5 P 3.9 0.18 5Sekisui & Roche Cobas Mira series 18 2.3 - 3.1 P 2.68 0.13 3.9 - 5.3 P 4.6 0.25 0.5 - 1.1 C 0.75 0.11 2.3 - 3.1 P 2.73 0.18 3.0 - 4.1 P 3.55 0.29 4Siemens Advia & Siemens Advia 19 2.4 - 3.3 P 2.88 0.07 4.4 - 6.0 P 5.2 0.13 0.3 - 0.9 C 0.6 0.07 2.7 - 3.7 P 3.19 0.08 3.1 - 4.2 P 3.66 0.6 8Siemens Dimension & Siemens Dim RxL, RxL Max 20 2.6 - 3.5 P 3.0 0.08 4.6 - 6.3 P 5.45 0.1 0.3 - 0.9 C 0.63 0.07 2.8 - 3.8 P 3.3 0.06 3.5 - 4.8 P 4.15 0.05 6Siemens Dimension & Siemens Dimension 21 2.5 - 3.3 P 2.9 0.17 4.4 - 6.0 P 5.23 0.3 0.3 - 0.9 C 0.57 0.05 2.7 - 3.6 P 3.17 0.2 3.4 - 4.6 P 4.0 0.24 7Siemens Dimension & Siemens Dimension EXL 22 2.5 - 3.4 P 2.97 0.09 4.5 - 6.2 P 5.35 0.15 0.3 - 0.9 C 0.62 0.08 2.8 - 3.7 P 3.24 0.12 3.5 - 4.7 P 4.09 0.13 49Siemens Dimension & Siemens Dimension Xpand 23 2.5 - 3.4 P 2.95 0.11 4.5 - 6.1 P 5.31 0.15 0.3 - 0.9 C 0.62 0.1 2.7 - 3.7 P 3.23 0.11 3.5 - 4.7 P 4.08 0.12 30Sterling Diagnostic & Other spectrophotometers 24 2.4 - 3.2 P 2.82 0.07 4.2 - 5.7 P 4.98 0.13 0.6 - 1.2 C 0.88 0.1 2.5 - 3.4 P 2.92 0.19 3.3 - 4.4 P 3.86 0.15 5Sterling Diagnostic & Pointe Sci 180/Sterl 2000 25 2.4 - 3.3 P 2.83 0.08 4.4 - 6.0 P 5.2 0.17 0.4 - 1.0 C 0.7 0.17 2.6 - 3.5 P 3.08 0.11 3.3 - 4.5 P 3.93 0.19 4Synermed & Synermed IR500 26 2.4 - 3.3 P 2.84 0.22 4.1 - 5.5 P 4.82 0.48 0.5 - 1.1 C 0.76 0.1 2.6 - 3.5 P 3.02 0.32 3.2 - 4.3 P 3.78 0.25 5Vital Diagnostic & Vital Diagnostic Eon 27 2.4 - 3.2 P 2.78 0.24 4.2 - 5.7 P 4.93 0.18 0.3 - 0.9 C 0.55 0.09 2.6 - 3.5 P 3.05 0.17 3.3 - 4.5 P 3.9 0.21 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 28 2.4 - 3.3 P 2.86 0.07 4.3 - 5.8 P 5.04 0.11 0.3 - 0.9 C 0.64 0.05 2.6 - 3.6 P 3.1 0.08 3.0 - 4.0 P 3.48 1.23 9Initial Grouping by ReagentAbbott Architect 29 2.6 - 3.5 P 3.08 0.05 4.7 - 6.4 P 5.58 0.1 0.4 - 1.0 C 0.71 0.04 2.9 - 3.9 P 3.42 0.05 3.5 - 4.8 P 4.14 0.09 26Alfa Wassermann 30 2.4 - 3.3 P 2.85 0.16 4.3 - 5.8 P 5.01 0.27 0.5 - 1.1 C 0.77 0.08 2.6 - 3.5 P 3.02 0.16 3.3 - 4.5 P 3.93 0.17 32Beckman Coulter 31 2.5 - 3.3 P 2.9 0.1 4.4 - 6.0 P 5.22 0.2 0.3 - 0.9 C 0.6 0.06 2.7 - 3.6 P 3.17 0.13 3.3 - 4.5 P 3.94 0.28 25Beckman Olympus 32 2.5 - 3.4 P 2.92 0.09 4.5 - 6.1 P 5.27 0.14 0.4 - 1.0 C 0.66 0.07 2.7 - 3.7 P 3.21 0.09 3.4 - 4.6 P 4.0 0.13 57Beckman Synchron IDMS trace 33 2.4 - 3.3 P 2.88 0.12 4.2 - 5.7 P 4.98 0.57 0.3 - 0.9 C 0.59 0.08 2.7 - 3.6 P 3.13 0.15 3.4 - 4.5 P 3.95 0.14 12Carolina 34 2.5 - 3.4 P 2.94 0.26 4.3 - 5.9 P 5.09 0.34 0.6 - 1.2 C 0.86 0.2 2.7 - 3.7 P 3.18 0.24 3.3 - 4.5 P 3.88 0.31 21Horiba ABX 35 2.4 - 3.3 P 2.84 0.13 4.3 - 5.9 P 5.09 0.11 0.4 - 1.0 C 0.71 0.1 2.7 - 3.6 P 3.12 0.09 3.3 - 4.5 P 3.89 0.12 9Medica 36 2.5 - 3.4 P 2.99 0.06 4.6 - 6.2 P 5.4 0.12 0.3 - 0.9 C 0.6 0.0 2.8 - 3.7 P 3.26 0.09 3.5 - 4.7 P 4.06 0.12 7Ortho Vitros 1-slide enz 37 2.5 - 3.4 P 2.96 0.13 4.6 - 6.3 P 5.45 1.31 0.4 - 1.0 C 0.69 0.06 2.6 - 3.5 P 3.03 0.11 3.5 - 4.8 P 4.13 0.15 19Ortho Vitros IDMS traceable 38 2.5 - 3.4 P 2.92 0.07 4.7 - 6.3 P 5.48 0.16 0.4 - 1.0 C 0.67 0.06 2.5 - 3.4 P 2.99 0.09 3.4 - 4.6 P 4.04 0.12 30Pointe Scientific 39 2.5 - 3.4 P 2.96 0.32 4.4 - 5.9 P 5.14 0.25 0.4 - 1.0 C 0.69 0.11 2.7 - 3.6 P 3.12 0.18 3.3 - 4.5 P 3.93 0.17 14Roche Cobas 40 2.4 - 3.3 P 2.85 0.13 4.4 - 5.9 P 5.12 0.17 0.3 - 0.9 C 0.64 0.05 2.6 - 3.5 P 3.06 0.16 3.4 - 4.6 P 3.96 0.18 43SDI Biomed 41 2.2 - 3.0 P 2.62 0.09 3.8 - 5.1 P 4.47 0.08 0.4 - 1.0 C 0.72 0.08 2.4 - 3.2 P 2.78 0.06 3.0 - 4.0 P 3.5 0.34 13Sekisui 42 2.4 - 3.3 P 2.85 0.22 4.1 - 5.6 P 4.83 0.31 0.5 - 1.1 C 0.82 0.1 2.5 - 3.4 P 2.97 0.27 3.2 - 4.3 P 3.77 0.29 10Siemens Advia 43 2.4 - 3.3 P 2.88 0.07 4.4 - 6.0 P 5.2 0.13 0.3 - 0.9 C 0.6 0.07 2.7 - 3.7 P 3.19 0.08 3.1 - 4.2 P 3.66 0.6 8Siemens Dimension 44 2.5 - 3.4 P 2.96 0.11 4.5 - 6.1 P 5.34 0.17 0.3 - 0.9 C 0.62 0.09 2.8 - 3.7 P 3.24 0.12 3.5 - 4.7 P 4.08 0.14 92Siemens EZCR/IDMS Traceable 45 2.5 - 3.3 P 2.9 0.09 4.5 - 6.0 P 5.25 0.16 0.3 - 0.9 C 0.59 0.03 2.7 - 3.6 P 3.17 0.11 3.4 - 4.6 P 3.97 0.12 13Siemens nonenz rate 46 2.5 - 3.4 P 2.99 0.07 4.6 - 6.2 P 5.36 0.1 0.4 - 1.0 C 0.65 0.06 2.8 - 3.7 P 3.26 0.07 3.5 - 4.7 P 4.11 0.08 14Sterling Diagnostic 47 2.4 - 3.2 P 2.82 0.08 4.3 - 5.8 P 5.08 0.19 0.5 - 1.1 C 0.8 0.16 2.5 - 3.4 P 2.99 0.18 3.3 - 4.5 P 3.89 0.17 9Synermed 48 2.4 - 3.3 P 2.83 0.2 4.1 - 5.6 P 4.87 0.45 0.4 - 1.0 C 0.72 0.13 2.6 - 3.5 P 3.03 0.3 3.2 - 4.4 P 3.82 0.24 6Vital Diagnostic 49 2.4 - 3.2 P 2.77 0.2 4.2 - 5.7 P 4.93 0.17 0.3 - 0.9 C 0.6 0.12 2.6 - 3.5 P 3.03 0.14 3.3 - 4.5 P 3.87 0.19 6Vital Diagnostics Envoy 50 2.4 - 3.3 P 2.86 0.07 4.3 - 5.8 P 5.04 0.11 0.3 - 0.9 C 0.64 0.05 2.6 - 3.6 P 3.1 0.08 3.3 - 4.5 P 3.91 0.09 9Initial Grouping by Sensitivity or PrincipleEnzymatic methods 52 2.5 - 3.4 P 2.96 0.11 4.5 - 6.2 P 5.35 0.54 0.3 - 0.9 C 0.63 0.09 2.7 - 3.7 P 3.21 0.14 3.5 - 4.7 P 4.09 0.14 121IDMS traceable 53 2.5 - 3.3 P 2.89 0.09 4.5 - 6.1 P 5.28 0.35 0.4 - 1.0 C 0.66 0.11 2.6 - 3.5 P 3.06 0.15 3.4 - 4.6 P 3.99 0.14 66Nonenzymatic endpoint 55 2.2 - 3.0 P 2.62 0.09 3.8 - 5.1 P 4.47 0.08 0.4 - 1.0 C 0.72 0.08 2.4 - 3.2 P 2.78 0.06 3.0 - 4.0 P 3.5 0.34 13Nonenzymatic rate 51 2.5 - 3.3 P 2.91 0.17 4.4 - 5.9 P 5.17 0.27 0.4 - 1.0 C 0.69 0.12 2.7 - 3.6 P 3.15 0.19 3.4 - 4.6 P 3.96 0.24 290Other 54 2.4 - 3.2 P 2.82 0.13 4.4 - 6.0 P 5.19 0.36 0.4 - 1.0 C 0.7 0.1 2.5 - 3.4 P 2.93 0.16 3.3 - 4.5 P 3.92 0.21 13Total PopulationWhole Population 56 2.5 - 3.3 P 2.91 0.16 4.4 - 6.0 P 5.21 0.4 0.4 - 1.0 C 0.68 0.28 2.7 - 3.6 P 3.14 0.2 3.4 - 4.6 P 3.98 0.26 506

GlucoseInitial Grouping by Reagent and InstrumentAbbott & Abbott Architect c, ci, i 1 107 - 131 P 118.7 1.1 180 - 220 P 200.0 2.7 86 - 105 P 95.8 1.1 97 - 118 P 107.6 1.0 158 - 194 P 176.1 2.1 27Alfa Wassermann & Alfa Wasser Axcel/Alera 2 110 - 135 P 122.4 2.8 183 - 224 P 203.2 4.5 89 - 109 P 99.1 2.3 99 - 121 P 110.4 2.5 163 - 200 P 181.6 3.9 30Beckman GO & Beck Coult Unicel DXC 3 105 - 128 P 116.4 3.3 178 - 218 P 198.0 4.3 85 - 103 P 94.0 2.3 94 - 115 P 104.4 2.9 158 - 193 P 175.8 4.3 5Beckman HK & Beck Coult Unicel DXC 4 105 - 129 P 117.1 1.7 178 - 218 P 198.2 2.6 85 - 104 P 94.9 1.5 95 - 116 P 105.8 2.2 159 - 194 P 176.5 2.3 13Beckman O2 electrode & Beck Coult Unicel DXC 5 104 - 127 P 115.7 1.4 178 - 217 P 197.4 1.8 84 - 103 P 93.4 1.4 94 - 114 P 103.9 1.6 157 - 192 P 174.4 1.8 7Beckman Olympus & Beck Olym AU 400/600/5400 6 107 - 130 P 118.4 3.4 180 - 221 P 200.5 5.7 85 - 104 P 94.9 2.9 95 - 116 P 105.9 2.9 160 - 195 P 177.7 5.9 38Beckman Olympus & Beck Olympus AU 2700 7 105 - 128 P 116.5 1.7 177 - 216 P 196.8 2.9 84 - 103 P 93.5 1.1 93 - 114 P 103.8 2.3 158 - 193 P 175.8 2.9 4Beckman Olympus & Beckman AU 480 8 108 - 131 P 119.5 2.6 181 - 222 P 201.4 3.9 86 - 105 P 95.6 2.6 96 - 117 P 106.4 3.6 161 - 197 P 178.7 5.1 15Carolina HK & Beckman Synchron CX3/7/9/L 9 108 - 133 P 120.5 4.7 180 - 220 P 200.3 5.9 89 - 109 P 99.0 4.1 99 - 121 P 110.3 3.5 161 - 197 P 179.0 1.2 4Horiba ABX & Horiba ABX 400 10 108 - 132 P 120.2 7.3 186 - 228 P 207.0 14.7 87 - 106 P 96.3 6.4 97 - 119 P 108.2 7.7 162 - 198 P 179.9 11.4 9Medica & Medica Easy RA 11 112 - 137 P 124.9 2.2 188 - 230 P 209.0 4.1 91 - 111 P 100.6 1.8 100 - 122 P 111.1 2.4 169 - 206 P 187.6 3.4 7Ortho Vitros & Ortho Vitros 3600, 5600 12 103 - 126 P 114.9 1.7 177 - 216 P 196.6 3.3 82 - 101 P 91.4 1.8 96 - 117 P 106.8 2.0 153 - 187 P 170.1 3.1 9

Ortho Vitros & Ortho Vitros 5,1 FS 13 105 - 128 P 116.7 2.7 176 - 215 P 195.7 3.1 83 - 101 P 91.8 2.1 96 - 118 P 107.2 2.4 152 - 186 P 169.2 3.3 6Ortho Vitros & Ortho Vitros not DT or ECi 14 105 - 128 P 116.5 2.3 176 - 215 P 195.7 3.6 83 - 102 P 92.6 1.9 97 - 118 P 107.7 1.8 152 - 186 P 169.0 3.1 33Roche Cobas & Roche Cobas Integra 15 106 - 130 P 118.0 2.7 181 - 221 P 201.1 4.6 86 - 105 P 95.3 2.2 96 - 117 P 106.4 2.7 161 - 197 P 179.0 3.5 14Roche Cobas & Roche e/c, 1XX, X000, Elec series 16 107 - 131 P 118.7 2.7 181 - 221 P 201.3 3.8 86 - 105 P 95.4 2.1 96 - 117 P 106.8 2.0 160 - 196 P 177.9 4.5 29SDI Biomed & SDI CA-240, 480 17 115 - 141 P 128.3 4.5 193 - 236 P 214.8 7.5 94 - 114 P 104.0 4.7 105 - 128 P 116.5 4.6 171 - 209 P 190.2 9.2 10Sekisui & Beck Olym AU 400/600/5400 18 109 - 133 P 121.0 6.0 183 - 223 P 202.8 7.7 89 - 109 P 98.7 4.0 100 - 122 P 111.2 5.4 162 - 198 P 180.2 6.9 6Sekisui & Roche Cobas Mira series 19 114 - 140 P 127.0 6.2 193 - 236 P 214.5 9.4 93 - 113 P 103.0 5.2 101 - 123 P 112.2 4.7 171 - 209 P 190.2 8.9 6Siemens HK & Siemens Advia 20 108 - 132 P 120.4 3.4 182 - 223 P 202.6 5.4 87 - 107 P 97.0 3.2 98 - 119 P 108.6 3.7 153 - 187 P 170.1 27.5 8Siemens HK & Siemens Dim RxL, RxL Max 21 110 - 134 P 121.8 1.0 182 - 223 P 202.4 1.5 87 - 107 P 97.2 1.2 101 - 123 P 111.8 1.0 160 - 196 P 177.8 2.2 5Siemens HK & Siemens Dimension 22 109 - 134 P 121.6 5.7 181 - 221 P 200.8 6.6 86 - 105 P 95.8 4.0 100 - 122 P 110.8 4.7 158 - 193 P 175.8 7.1 5Siemens HK & Siemens Dimension EXL 23 110 - 134 P 122.1 2.1 183 - 224 P 203.2 3.2 87 - 107 P 97.2 1.9 100 - 123 P 111.6 2.2 161 - 197 P 178.9 3.5 34Siemens HK & Siemens Dimension Xpand 24 109 - 134 P 121.6 1.9 183 - 223 P 203.1 3.0 87 - 107 P 97.0 1.7 100 - 123 P 111.5 2.4 160 - 195 P 177.7 2.4 20Siemens HK, GLUC & Siemens Dim RxL, RxL Max 25 112 - 137 P 124.6 2.1 186 - 227 P 206.4 2.7 90 - 110 P 99.6 1.6 103 - 126 P 114.4 1.2 163 - 200 P 181.4 2.2 5Siemens HK, GLUC & Siemens Dimension EXL 26 110 - 134 P 122.0 2.9 182 - 223 P 202.6 3.9 87 - 107 P 97.2 2.1 100 - 123 P 111.6 2.4 160 - 196 P 178.3 3.3 26Siemens HK, GLUC & Siemens Dimension Xpand 27 110 - 135 P 122.3 3.1 183 - 224 P 203.3 5.2 87 - 107 P 97.2 2.6 101 - 124 P 112.3 3.3 161 - 196 P 178.5 4.7 21Sterling Diagnostic & Other spectrophotometers 28 105 - 128 P 116.4 2.9 175 - 214 P 194.5 5.3 86 - 105 P 95.3 2.7 95 - 116 P 105.1 3.9 158 - 193 P 175.0 3.9 8Sterling Diagnostic & Pointe Sci 180/Sterl 2000 29 103 - 125 P 114.0 3.2 171 - 209 P 189.8 9.1 84 - 103 P 93.4 3.0 93 - 113 P 102.8 2.9 154 - 188 P 171.2 2.8 5Synermed & Synermed IR500 30 109 - 133 P 121.3 1.1 183 - 224 P 203.3 1.9 86 - 105 P 95.5 1.5 98 - 120 P 109.3 1.5 162 - 197 P 179.5 2.2 4Vital Diagnostic & Vital Diagnostic Eon 31 116 - 142 P 129.0 3.3 190 - 232 P 210.8 7.7 94 - 115 P 104.8 1.9 105 - 128 P 116.8 1.8 170 - 207 P 188.5 1.5 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 32 113 - 139 P 126.0 2.8 189 - 231 P 210.4 4.9 93 - 114 P 103.8 2.3 102 - 125 P 113.6 2.3 150 - 183 P 166.2 58.9 9Initial Grouping by ReagentAbbott 33 107 - 131 P 118.7 1.1 180 - 220 P 200.0 2.7 86 - 105 P 95.8 1.1 97 - 118 P 107.6 1.0 158 - 194 P 176.1 2.1 27Alfa Wassermann 34 110 - 134 P 121.9 3.3 183 - 223 P 203.0 4.5 89 - 109 P 98.8 2.3 99 - 121 P 110.2 2.5 163 - 199 P 181.2 3.9 34Beckman GO 35 105 - 128 P 116.4 3.3 178 - 218 P 198.0 4.3 85 - 103 P 94.0 2.3 94 - 115 P 104.4 2.9 158 - 193 P 175.8 4.3 5Beckman HK 36 106 - 130 P 117.9 2.4 180 - 220 P 200.2 4.9 86 - 105 P 95.3 1.7 96 - 117 P 106.6 2.4 160 - 196 P 178.2 4.0 19Beckman O2 electrode 37 104 - 127 P 115.9 1.4 178 - 218 P 197.8 1.9 84 - 103 P 93.8 1.6 94 - 115 P 104.3 1.8 157 - 192 P 174.5 1.7 8Beckman Olympus 38 107 - 131 P 118.7 3.2 181 - 221 P 200.6 5.2 86 - 105 P 95.1 2.8 95 - 117 P 106.0 3.1 160 - 196 P 178.0 5.5 59Carolina HK 39 109 - 133 P 121.3 6.1 183 - 224 P 203.2 8.4 89 - 109 P 98.9 4.3 100 - 122 P 110.7 4.9 163 - 199 P 181.3 6.2 15Horiba ABX 40 108 - 132 P 120.2 7.3 186 - 228 P 207.0 14.7 87 - 106 P 96.3 6.4 97 - 119 P 108.2 7.7 162 - 198 P 179.9 11.4 9Medica 41 112 - 137 P 124.9 2.2 188 - 230 P 209.0 4.1 91 - 111 P 100.6 1.8 100 - 122 P 111.1 2.4 169 - 206 P 187.6 3.4 7Ortho Vitros 42 105 - 128 P 116.4 2.3 176 - 216 P 196.1 3.4 83 - 102 P 92.3 1.9 97 - 118 P 107.6 2.0 152 - 186 P 169.4 3.1 53Pointe Sci HK 43 108 - 132 P 119.6 2.5 182 - 222 P 201.7 5.6 89 - 109 P 98.9 3.6 98 - 120 P 108.9 3.2 160 - 195 P 177.6 7.5 14Roche Cobas 44 107 - 130 P 118.6 2.7 181 - 222 P 201.4 4.0 86 - 105 P 95.4 2.1 96 - 117 P 106.7 2.2 160 - 196 P 178.3 4.2 47SDI Biomed 45 116 - 142 P 128.9 4.7 192 - 234 P 212.9 9.1 93 - 114 P 103.3 4.8 104 - 127 P 115.7 4.9 170 - 208 P 188.8 10.3 12Sekisui 46 111 - 136 P 123.6 6.4 187 - 228 P 207.7 9.9 90 - 111 P 100.5 4.8 100 - 123 P 111.4 4.8 166 - 203 P 184.2 9.0 14Siemens HK 47 110 - 134 P 121.7 2.6 183 - 223 P 202.9 3.7 87 - 107 P 97.0 2.2 100 - 122 P 111.2 2.8 160 - 195 P 177.3 10.2 72Siemens HK, GLUC 48 110 - 135 P 122.3 3.0 183 - 224 P 203.3 4.4 88 - 107 P 97.3 2.4 101 - 123 P 112.2 2.8 161 - 197 P 178.7 3.9 55Sterling Diagnostic 49 104 - 127 P 115.9 3.4 174 - 212 P 192.8 7.1 85 - 104 P 94.6 2.9 94 - 115 P 104.2 3.6 157 - 192 P 174.1 4.4 14Synermed 50 109 - 133 P 120.8 1.3 183 - 224 P 203.4 1.7 86 - 105 P 95.8 1.5 98 - 120 P 109.4 1.4 162 - 198 P 179.8 2.0 5Vital Diagnostic 51 113 - 138 P 125.3 5.8 187 - 228 P 207.3 8.0 92 - 113 P 102.3 3.8 102 - 125 P 113.5 4.8 165 - 202 P 183.7 7.0 6Vital Diagnostics Envoy 52 113 - 139 P 126.0 2.8 189 - 231 P 210.4 4.9 93 - 114 P 103.8 2.3 102 - 125 P 113.6 2.3 168 - 206 P 187.0 4.5 9Initial Grouping by Sensitivity or PrincipleGlucose Oxidase (GO) 55 106 - 129 P 117.6 3.8 178 - 217 P 197.7 6.4 85 - 104 P 94.1 3.5 97 - 118 P 107.4 3.2 156 - 191 P 173.3 6.7 91HexoKinase (HK) high recov 54 110 - 135 P 122.5 4.1 184 - 224 P 204.0 6.2 90 - 110 P 99.8 3.4 100 - 122 P 110.8 3.5 163 - 200 P 181.4 6.0 67HexoKinase (HK) low recov 53 109 - 133 P 120.6 4.2 182 - 223 P 202.6 6.3 87 - 106 P 96.7 3.4 98 - 120 P 109.3 4.3 161 - 197 P 178.7 7.3 333Oxygen electrode 56 104 - 127 P 115.9 1.4 178 - 218 P 197.8 1.9 84 - 103 P 93.8 1.6 94 - 115 P 104.3 1.8 157 - 192 P 174.5 1.7 8Other 57 107 - 130 P 118.5 5.0 179 - 219 P 199.4 7.9 86 - 105 P 95.1 4.9 96 - 118 P 107.1 5.9 158 - 193 P 175.7 9.0 11Total PopulationWhole Population 58 108 - 132 P 120.0 4.9 181 - 222 P 201.5 7.2 87 - 106 P 96.5 4.0 98 - 120 P 109.0 4.4 160 - 195 P 177.7 8.5 510

PhosphorusInitial Grouping by Reagent and InstrumentAbbott Architect & Abbott Architect c, ci, i 1 3.3 - 4.4 P 3.84 0.09 1.9 - 2.7 C 2.34 0.11 7.9 - 10.7 P 9.29 0.29 3.0 - 4.1 P 3.54 0.13 2.8 - 3.8 P 3.34 0.07 22Alfa Wassermann & Alfa Wasser Axcel/Alera 2 3.4 - 4.6 P 4.02 0.11 1.9 - 2.7 C 2.28 0.12 8.2 - 11.1 P 9.63 0.23 3.0 - 4.1 P 3.58 0.12 2.9 - 3.9 P 3.39 0.08 8Beckman Olympus blanked & Beck Olym AU400/600/5400 3 3.2 - 4.3 P 3.75 0.1 1.9 - 2.7 C 2.28 0.14 7.8 - 10.5 P 9.12 0.46 2.9 - 4.0 P 3.46 0.13 2.8 - 3.7 P 3.25 0.1 13

Beckman Olympus blanked & Beckman AU 480 4 3.2 - 4.3 P 3.78 0.16 1.9 - 2.7 C 2.3 0.11 7.5 - 10.2 P 8.86 0.29 3.0 - 4.0 P 3.48 0.19 2.8 - 3.7 P 3.24 0.14 5Beckman Olympus no blank & Beck Olym AU400/600/5400 5 3.2 - 4.4 P 3.79 0.19 2.0 - 2.8 C 2.35 0.19 7.6 - 10.3 P 8.95 0.36 3.1 - 4.1 P 3.59 0.34 2.8 - 3.7 P 3.26 0.15 17

Beckman Olympus no blank & Beckman AU 480 6 3.2 - 4.3 P 3.78 0.08 1.9 - 2.7 C 2.31 0.1 7.7 - 10.4 P 9.06 0.14 3.0 - 4.0 P 3.51 0.11 2.8 - 3.8 P 3.3 0.11 9Beckman Synchron PHS & Beck Coult Unicel DXC 7 3.4 - 4.5 P 3.95 0.11 2.1 - 2.9 C 2.5 0.11 8.0 - 10.9 P 9.46 0.17 3.1 - 4.2 P 3.69 0.11 2.9 - 4.0 P 3.46 0.13 15Beckman UV/rate & Beck Coult Unicel DXC 8 3.5 - 4.7 P 4.07 0.15 2.2 - 3.0 C 2.63 0.11 8.0 - 10.8 P 9.38 0.16 3.3 - 4.5 P 3.88 0.15 3.0 - 4.1 P 3.58 0.13 4

Carolina & Beck Olym AU 400/600/5400 9 3.5 - 4.7 P 4.1 0.12 2.0 - 2.8 C 2.42 0.08 8.3 - 11.2 P 9.78 0.52 3.4 - 4.5 P 3.95 0.09 3.1 - 4.2 P 3.63 0.08 4Carolina & Beckman Synchron CX3/7/9/L 10 3.6 - 4.9 P 4.28 0.31 2.3 - 3.1 C 2.65 0.24 7.8 - 10.5 P 9.15 0.38 3.4 - 4.6 P 4.03 0.27 3.1 - 4.3 P 3.7 0.24 6Horiba ABX & Horiba ABX 400 11 3.5 - 4.8 P 4.17 0.26 2.4 - 3.3 P 2.83 0.21 7.9 - 10.7 P 9.28 0.48 3.4 - 4.6 P 3.97 0.26 3.1 - 4.2 P 3.65 0.25 6Ortho Vitros & Ortho Vitros 3600, 5600 12 3.5 - 4.7 P 4.09 0.11 2.4 - 3.3 P 2.87 0.11 7.5 - 10.2 P 8.86 0.34 3.4 - 4.6 P 4.01 0.13 3.2 - 4.3 P 3.72 0.11 9Ortho Vitros & Ortho Vitros 5,1 FS 13 3.5 - 4.8 P 4.15 0.05 2.4 - 3.3 P 2.87 0.16 7.4 - 9.9 P 8.65 0.1 3.4 - 4.5 P 3.95 0.15 3.1 - 4.2 P 3.67 0.09 6Ortho Vitros & Ortho Vitros not DT or ECi 14 3.5 - 4.8 P 4.16 0.16 2.5 - 3.3 P 2.89 0.14 7.5 - 10.2 P 8.86 0.31 3.4 - 4.6 P 3.99 0.16 3.2 - 4.3 P 3.76 0.13 23Roche Cobas & Roche e/c, 1XX, X000, Elec series 15 3.3 - 4.5 P 3.88 0.1 2.0 - 2.8 C 2.38 0.11 8.0 - 10.8 P 9.41 0.26 3.1 - 4.2 P 3.63 0.14 2.9 - 3.9 P 3.38 0.12 19Roche Cobas Integra & Roche Cobas Integra 16 3.3 - 4.5 P 3.93 0.04 2.0 - 2.8 C 2.4 0.1 8.1 - 11.0 P 9.54 0.23 3.1 - 4.2 P 3.64 0.13 2.9 - 3.9 P 3.41 0.09 8SDI Biomed & SDI CA-240, 480 17 3.5 - 4.7 P 4.12 0.07 2.3 - 3.1 P 2.73 0.17 7.7 - 10.4 P 9.05 0.14 3.3 - 4.5 P 3.88 0.13 3.1 - 4.2 P 3.63 0.11 6Sekisui & Beck Olym AU 400/600/5400 18 3.3 - 4.5 P 3.92 0.2 2.0 - 2.8 C 2.43 0.18 7.9 - 10.7 P 9.32 0.52 3.1 - 4.1 P 3.6 0.19 2.9 - 3.9 P 3.4 0.17 6Siemens Advia & Siemens Advia 19 3.3 - 4.5 P 3.93 0.1 2.0 - 2.8 C 2.44 0.09 8.0 - 10.8 P 9.43 0.09 3.1 - 4.3 P 3.7 0.11 2.9 - 3.9 P 3.41 0.1 7Siemens Dimension & Siemens Dim RxL, RxL Max 20 3.3 - 4.4 P 3.84 0.17 1.9 - 2.7 C 2.26 0.17 7.8 - 10.6 P 9.23 0.15 3.0 - 4.0 P 3.51 0.18 2.8 - 3.8 P 3.28 0.15 8Siemens Dimension & Siemens Dimension 21 3.4 - 4.6 P 4.0 0.18 2.1 - 2.9 C 2.52 0.2 8.1 - 10.9 P 9.48 0.15 3.2 - 4.3 P 3.74 0.25 2.9 - 3.9 P 3.42 0.23 5Siemens Dimension & Siemens Dimension EXL 22 3.3 - 4.4 P 3.87 0.17 2.0 - 2.8 C 2.36 0.17 7.9 - 10.6 P 9.26 0.27 3.0 - 4.1 P 3.56 0.18 2.8 - 3.8 P 3.33 0.16 51Siemens Dimension & Siemens Dimension Xpand 23 3.3 - 4.5 P 3.88 0.2 2.0 - 2.8 C 2.37 0.18 7.8 - 10.6 P 9.19 0.38 3.0 - 4.1 P 3.56 0.21 2.8 - 3.8 P 3.33 0.18 26Initial Grouping by ReagentAbbott Architect 24 3.3 - 4.4 P 3.84 0.09 1.9 - 2.7 C 2.34 0.11 7.9 - 10.7 P 9.29 0.29 3.0 - 4.1 P 3.54 0.13 2.8 - 3.8 P 3.34 0.07 22Alfa Wassermann 25 3.4 - 4.6 P 4.02 0.11 1.9 - 2.7 C 2.28 0.12 8.2 - 11.1 P 9.63 0.23 3.0 - 4.1 P 3.58 0.12 2.9 - 3.9 P 3.39 0.08 8Beckman Olympus blanked 26 3.2 - 4.3 P 3.76 0.12 1.9 - 2.7 C 2.29 0.12 7.7 - 10.4 P 9.03 0.42 2.9 - 4.0 P 3.46 0.14 2.8 - 3.7 P 3.25 0.11 21Beckman Olympus no blank 27 3.2 - 4.4 P 3.79 0.16 1.9 - 2.7 C 2.33 0.17 7.6 - 10.3 P 8.99 0.3 3.0 - 4.1 P 3.56 0.27 2.8 - 3.8 P 3.28 0.14 29Beckman Synchron PHS 28 3.3 - 4.5 P 3.94 0.12 2.1 - 2.9 C 2.49 0.12 8.1 - 10.9 P 9.47 0.19 3.1 - 4.2 P 3.69 0.15 2.9 - 4.0 P 3.46 0.14 18Beckman UV/rate 29 3.4 - 4.6 P 4.02 0.17 2.2 - 3.0 C 2.62 0.1 7.8 - 10.5 P 9.14 0.49 3.3 - 4.4 P 3.86 0.14 3.0 - 4.0 P 3.5 0.19 5Carolina 30 3.5 - 4.8 P 4.14 0.29 2.1 - 2.9 C 2.53 0.32 7.8 - 10.5 P 9.13 1.08 3.3 - 4.5 P 3.92 0.31 3.0 - 4.1 P 3.58 0.26 17Horiba ABX 31 3.5 - 4.8 P 4.17 0.26 2.4 - 3.3 P 2.83 0.21 7.9 - 10.7 P 9.28 0.48 3.4 - 4.6 P 3.97 0.26 3.1 - 4.2 P 3.65 0.25 6Ortho Vitros 32 3.5 - 4.8 P 4.14 0.14 2.4 - 3.3 P 2.88 0.13 7.5 - 10.1 P 8.82 0.31 3.4 - 4.6 P 3.99 0.15 3.2 - 4.3 P 3.73 0.13 39Pointe Scientific 33 3.3 - 4.5 P 3.92 0.04 2.2 - 3.0 C 2.56 0.15 7.9 - 10.7 P 9.34 0.36 3.1 - 4.2 P 3.68 0.13 2.9 - 4.0 P 3.46 0.08 5Roche Cobas 34 3.3 - 4.5 P 3.89 0.11 2.0 - 2.8 C 2.39 0.1 8.0 - 10.8 P 9.37 0.25 3.1 - 4.2 P 3.64 0.13 2.9 - 3.9 P 3.38 0.11 25Roche Cobas Integra 35 3.3 - 4.5 P 3.9 0.09 2.0 - 2.8 C 2.37 0.11 8.0 - 10.9 P 9.46 0.24 3.0 - 4.1 P 3.58 0.16 2.9 - 3.9 P 3.37 0.11 11SDI Biomed 36 3.5 - 4.7 P 4.13 0.07 2.3 - 3.2 P 2.74 0.16 7.7 - 10.4 P 9.04 0.13 3.3 - 4.5 P 3.87 0.13 3.1 - 4.2 P 3.61 0.11 7Sekisui 37 3.4 - 4.6 P 3.97 0.23 2.1 - 2.9 C 2.48 0.2 8.0 - 10.8 P 9.42 0.54 3.1 - 4.2 P 3.66 0.3 2.9 - 4.0 P 3.44 0.28 9Siemens Advia 38 3.3 - 4.5 P 3.93 0.1 2.0 - 2.8 C 2.44 0.09 8.0 - 10.8 P 9.43 0.09 3.1 - 4.3 P 3.7 0.11 2.9 - 3.9 P 3.41 0.1 7Siemens Dimension 39 3.3 - 4.5 P 3.87 0.18 2.0 - 2.8 C 2.37 0.18 7.9 - 10.6 P 9.25 0.3 3.0 - 4.1 P 3.57 0.19 2.8 - 3.8 P 3.33 0.17 90Initial Grouping by Sensitivity or PrincipleOther 45 3.3 - 4.5 P 3.93 0.24 2.0 - 2.8 C 2.37 0.14 8.0 - 10.8 P 9.43 0.84 3.1 - 4.1 P 3.6 0.28 2.8 - 3.8 P 3.34 0.21 7UV-bichromatic-no sam blks 40 3.3 - 4.5 P 3.9 0.21 2.0 - 2.8 C 2.42 0.24 7.7 - 10.5 P 9.09 0.38 3.1 - 4.2 P 3.65 0.28 2.9 - 3.9 P 3.37 0.18 49UV-bichromatic-sam blanked 41 3.3 - 4.5 P 3.89 0.2 2.0 - 2.8 C 2.37 0.19 7.9 - 10.6 P 9.24 0.45 3.1 - 4.1 P 3.6 0.22 2.8 - 3.9 P 3.35 0.18 176UV-just sample blanked 42 3.3 - 4.5 P 3.94 0.12 2.1 - 2.9 C 2.48 0.12 8.0 - 10.9 P 9.46 0.2 3.1 - 4.2 P 3.68 0.15 2.9 - 4.0 P 3.45 0.14 20UV-rate 43 3.4 - 4.6 P 4.01 0.2 2.2 - 3.0 C 2.56 0.24 8.0 - 10.8 P 9.37 0.41 3.2 - 4.3 P 3.76 0.25 3.0 - 4.0 P 3.48 0.21 23UV-uncorrected 44 3.3 - 4.5 P 3.92 0.23 2.0 - 2.8 C 2.42 0.26 8.0 - 10.8 P 9.37 0.46 3.1 - 4.2 P 3.64 0.34 2.9 - 3.9 P 3.38 0.31 28Visible 46 3.5 - 4.8 P 4.14 0.14 2.4 - 3.3 P 2.87 0.14 7.5 - 10.2 P 8.84 0.34 3.4 - 4.6 P 3.98 0.16 3.2 - 4.3 P 3.73 0.14 41Total PopulationWhole Population 47 3.4 - 4.6 P 3.96 0.62 2.1 - 2.9 C 2.51 0.89 7.8 - 10.6 P 9.18 0.71 3.1 - 4.2 P 3.69 0.49 2.9 - 4.0 P 3.44 0.46 344

PotassiumInitial Grouping by Reagent and InstrumentAVL & Roche/AVL 9100 1 4.1 - 5.1 C 4.58 0.08 2.9 - 3.9 C 3.4 0.23 3.9 - 4.9 C 4.35 0.05 4.9 - 5.9 C 5.4 0.0 2.6 - 3.6 C 3.1 0.07 4Abbott Architect & Abbott Architect c, ci, i 2 4.1 - 5.1 C 4.55 0.07 2.8 - 3.8 C 3.32 0.05 4.1 - 5.1 C 4.55 0.06 4.7 - 5.7 C 5.24 0.06 2.7 - 3.7 C 3.16 0.06 26Alfa Wassermann & Alfa Wasser Axcel/Alera 3 4.1 - 5.1 C 4.64 0.06 2.9 - 3.9 C 3.36 0.07 4.0 - 5.0 C 4.45 0.11 4.9 - 5.9 C 5.42 0.07 2.7 - 3.7 C 3.15 0.07 26Beckman Coulter & Beck Coult Unicel DXC 4 4.0 - 5.0 C 4.45 0.06 2.7 - 3.7 C 3.2 0.06 3.9 - 4.9 C 4.44 0.07 4.7 - 5.7 C 5.2 0.04 2.6 - 3.6 C 3.05 0.05 26Beckman Coulter & Beck Olym AU 400/600/5400 5 4.0 - 5.0 C 4.5 0.12 2.8 - 3.8 C 3.25 0.05 4.0 - 5.0 C 4.53 0.04 4.6 - 5.6 C 5.13 0.08 2.6 - 3.6 C 3.08 0.04 4Beckman Coulter & Beckman AU 480 6 4.0 - 5.0 C 4.48 0.08 2.8 - 3.8 C 3.28 0.08 4.1 - 5.1 C 4.55 0.05 4.7 - 5.7 C 5.15 0.05 2.7 - 3.7 C 3.18 0.04 4Beckman Olympus & Beck Olym AU 400/600/5400 7 4.0 - 5.0 C 4.45 0.06 2.8 - 3.8 C 3.25 0.05 4.0 - 5.0 C 4.5 0.1 4.6 - 5.6 C 5.12 0.07 2.7 - 3.7 C 3.15 0.06 42Beckman Olympus & Beck Olympus AU 2700 8 4.0 - 5.0 C 4.53 0.04 2.8 - 3.8 C 3.28 0.04 4.1 - 5.1 C 4.58 0.04 4.7 - 5.7 C 5.18 0.08 2.7 - 3.7 C 3.15 0.05 4Beckman Olympus & Beckman AU 480 9 3.9 - 4.9 C 4.44 0.06 2.8 - 3.8 C 3.25 0.05 4.0 - 5.0 C 4.46 0.06 4.6 - 5.6 C 5.11 0.06 2.6 - 3.6 C 3.09 0.02 15Carolina & Beckman Synchron CX3/7/9/L 10 4.1 - 5.1 C 4.59 0.07 2.9 - 3.9 C 3.37 0.12 4.1 - 5.1 C 4.6 0.09 4.8 - 5.8 C 5.29 0.1 2.7 - 3.7 C 3.22 0.12 9Horiba ABX & Horiba ABX 400 11 4.0 - 5.0 C 4.52 0.06 2.9 - 3.9 C 3.4 0.07 3.8 - 4.8 C 4.28 0.08 4.8 - 5.8 C 5.26 0.08 2.7 - 3.7 C 3.2 0.07 9Medica Easy RA & Medica Easy RA 12 3.9 - 4.9 C 4.43 0.18 2.8 - 3.8 C 3.3 0.0 3.7 - 4.7 C 4.22 0.04 4.8 - 5.8 C 5.26 0.07 2.6 - 3.6 C 3.09 0.03 8Medica EasyLyte & Medica EasyLyte series 13 4.0 - 5.0 C 4.48 0.04 3.0 - 4.0 C 3.52 0.44 3.9 - 4.9 C 4.44 0.48 4.8 - 5.8 C 5.28 0.04 2.6 - 3.6 C 3.1 0.0 5Ortho Vitros & Ortho Vitros 3600, 5600 14 4.2 - 5.2 C 4.69 0.06 3.0 - 4.0 C 3.46 0.05 3.9 - 4.9 C 4.44 0.09 5.0 - 6.0 C 5.53 0.11 2.7 - 3.7 C 3.24 0.09 8Ortho Vitros & Ortho Vitros 5,1 FS 15 4.2 - 5.2 C 4.65 0.08 3.0 - 4.0 C 3.45 0.05 3.9 - 4.9 C 4.43 0.09 5.0 - 6.0 C 5.47 0.09 2.7 - 3.7 C 3.18 0.07 6Ortho Vitros & Ortho Vitros not DT or ECi 16 4.2 - 5.2 C 4.68 0.07 3.0 - 4.0 C 3.46 0.05 3.9 - 4.9 C 4.42 0.06 5.0 - 6.0 C 5.49 0.07 2.7 - 3.7 C 3.22 0.05 33Roche Cobas dil ISE & Roche Cobas Integra 17 4.0 - 5.0 C 4.54 0.05 2.8 - 3.8 C 3.3 0.0 4.0 - 5.0 C 4.52 0.09 4.7 - 5.7 C 5.23 0.05 2.6 - 3.6 C 3.14 0.05 13

Roche Cobas dil ISE & Roche e/c, 1XX, X000, Elecseries 18 4.1 - 5.1 C 4.56 0.1 2.8 - 3.8 C 3.34 0.11 4.1 - 5.1 C 4.58 0.1 4.8 - 5.8 C 5.31 0.11 2.7 - 3.7 C 3.17 0.1 22

Roche Cobas und ISE & Roche e/c, 1XX, X000, Elecseries 19 4.1 - 5.1 C 4.57 0.09 2.8 - 3.8 C 3.32 0.12 4.1 - 5.1 C 4.55 0.16 4.8 - 5.8 C 5.27 0.14 2.7 - 3.7 C 3.2 0.14 6

SDI Biomed & SDI CA-240, 480 20 3.9 - 4.9 C 4.43 0.08 2.8 - 3.8 C 3.31 0.08 3.8 - 4.8 C 4.31 0.08 4.7 - 5.7 C 5.19 0.18 2.7 - 3.7 C 3.16 0.07 10Siemens QuickLYTE IMT & Siemens Dim RxL, RxLMax 21 4.0 - 5.0 C 4.47 0.08 2.7 - 3.7 C 3.22 0.06 3.9 - 4.9 C 4.44 0.07 4.6 - 5.6 C 5.07 0.2 2.6 - 3.6 C 3.12 0.07 10

Siemens QuickLYTE IMT & Siemens Dimension 22 4.0 - 5.0 C 4.54 0.05 2.8 - 3.8 C 3.26 0.05 4.0 - 5.0 C 4.51 0.08 4.7 - 5.7 C 5.24 0.05 2.6 - 3.6 C 3.1 0.0 8Siemens QuickLYTE IMT & Siemens Dimension EXL 23 4.0 - 5.0 C 4.52 0.05 2.8 - 3.8 C 3.25 0.05 4.0 - 5.0 C 4.47 0.06 4.7 - 5.7 C 5.21 0.04 2.6 - 3.6 C 3.09 0.03 60Siemens QuickLYTE IMT & Siemens DimensionXpand 24 4.0 - 5.0 C 4.54 0.06 2.8 - 3.8 C 3.25 0.06 4.0 - 5.0 C 4.5 0.06 4.7 - 5.7 C 5.24 0.06 2.6 - 3.6 C 3.1 0.04 41

Siemens dil ISE & Siemens Advia 25 4.1 - 5.1 C 4.59 0.06 2.8 - 3.8 C 3.33 0.05 4.1 - 5.1 C 4.59 0.14 4.8 - 5.8 C 5.34 0.09 2.7 - 3.7 C 3.23 0.05 7Vital Diagnostic Eon & Vital Diagnostic Eon 26 3.8 - 4.8 C 4.3 0.07 2.9 - 3.9 C 3.4 0.41 3.6 - 4.6 C 4.08 0.04 4.5 - 5.5 C 5.0 0.12 2.5 - 3.5 C 2.95 0.05 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 27 3.8 - 4.8 C 4.34 0.11 2.7 - 3.7 C 3.16 0.1 3.7 - 4.7 C 4.2 0.11 4.5 - 5.5 C 5.0 0.15 2.5 - 3.5 C 3.0 0.08 10Initial Grouping by ReagentAVL 28 4.1 - 5.1 C 4.58 0.07 2.9 - 3.9 C 3.38 0.21 3.9 - 4.9 C 4.36 0.05 4.9 - 5.9 C 5.4 0.0 2.6 - 3.6 C 3.08 0.07 5Abbott Architect 29 4.1 - 5.1 C 4.55 0.07 2.8 - 3.8 C 3.32 0.05 4.1 - 5.1 C 4.55 0.06 4.7 - 5.7 C 5.24 0.06 2.7 - 3.7 C 3.16 0.06 26Alfa Wassermann 30 4.1 - 5.1 C 4.64 0.06 2.9 - 3.9 C 3.36 0.08 3.9 - 4.9 C 4.44 0.11 4.9 - 5.9 C 5.42 0.07 2.6 - 3.6 C 3.14 0.08 29Beckman Coulter 31 4.0 - 5.0 C 4.46 0.08 2.7 - 3.7 C 3.22 0.06 4.0 - 5.0 C 4.46 0.08 4.7 - 5.7 C 5.18 0.06 2.6 - 3.6 C 3.07 0.06 36Beckman Olympus 32 4.0 - 5.0 C 4.45 0.06 2.8 - 3.8 C 3.25 0.05 4.0 - 5.0 C 4.49 0.09 4.6 - 5.6 C 5.12 0.07 2.6 - 3.6 C 3.13 0.06 63Carolina 33 4.0 - 5.0 C 4.51 0.16 2.8 - 3.8 C 3.32 0.12 4.0 - 5.0 C 4.47 0.2 4.7 - 5.7 C 5.21 0.14 2.7 - 3.7 C 3.17 0.13 15Diamond Diagnostics 34 3.9 - 4.9 C 4.44 0.1 2.7 - 3.7 C 3.22 0.07 3.9 - 4.9 C 4.44 0.08 4.6 - 5.6 C 5.1 0.06 2.6 - 3.6 C 3.1 0.09 5Horiba ABX 35 4.0 - 5.0 C 4.52 0.06 2.9 - 3.9 C 3.4 0.07 3.8 - 4.8 C 4.28 0.08 4.8 - 5.8 C 5.26 0.08 2.7 - 3.7 C 3.2 0.07 9Medica Easy RA 36 3.9 - 4.9 C 4.43 0.18 2.8 - 3.8 C 3.3 0.0 3.7 - 4.7 C 4.22 0.04 4.8 - 5.8 C 5.26 0.07 2.6 - 3.6 C 3.09 0.03 8Medica EasyLyte 37 4.0 - 5.0 C 4.5 0.09 3.0 - 4.0 C 3.46 0.34 3.9 - 4.9 C 4.38 0.37 4.8 - 5.8 C 5.26 0.12 2.6 - 3.6 C 3.14 0.07 9Ortho Vitros 38 4.2 - 5.2 C 4.67 0.08 3.0 - 4.0 C 3.46 0.05 3.9 - 4.9 C 4.42 0.07 5.0 - 6.0 C 5.5 0.08 2.7 - 3.7 C 3.22 0.06 52Pointe Scientific 39 3.8 - 4.8 C 4.34 0.17 2.8 - 3.8 C 3.28 0.17 3.9 - 4.9 C 4.4 0.24 4.5 - 5.5 C 5.04 0.2 2.6 - 3.6 C 3.06 0.05 5Roche Cobas dil ISE 40 4.1 - 5.1 C 4.56 0.09 2.8 - 3.8 C 3.33 0.09 4.1 - 5.1 C 4.56 0.11 4.8 - 5.8 C 5.29 0.1 2.7 - 3.7 C 3.16 0.08 38Roche Cobas und ISE 41 4.1 - 5.1 C 4.55 0.08 2.8 - 3.8 C 3.32 0.1 4.0 - 5.0 C 4.51 0.16 4.8 - 5.8 C 5.25 0.12 2.7 - 3.7 C 3.17 0.12 11SDI Biomed 42 3.9 - 4.9 C 4.43 0.07 2.8 - 3.8 C 3.31 0.08 3.8 - 4.8 C 4.29 0.1 4.7 - 5.7 C 5.18 0.17 2.7 - 3.7 C 3.17 0.07 12Siemens QuickLYTE IMT 43 4.0 - 5.0 C 4.52 0.06 2.8 - 3.8 C 3.25 0.05 4.0 - 5.0 C 4.48 0.07 4.7 - 5.7 C 5.21 0.09 2.6 - 3.6 C 3.1 0.04 120Siemens dil ISE 44 4.1 - 5.1 C 4.59 0.06 2.8 - 3.8 C 3.33 0.05 4.1 - 5.1 C 4.59 0.14 4.8 - 5.8 C 5.34 0.09 2.7 - 3.7 C 3.23 0.05 7Vital Diagnostic Eon 45 3.8 - 4.8 C 4.3 0.07 2.9 - 3.9 C 3.4 0.41 3.6 - 4.6 C 4.08 0.04 4.5 - 5.5 C 5.0 0.12 2.5 - 3.5 C 2.95 0.05 4Vital Diagnostics Envoy 46 3.8 - 4.8 C 4.34 0.11 2.7 - 3.7 C 3.16 0.1 3.7 - 4.7 C 4.2 0.11 4.5 - 5.5 C 5.0 0.15 2.5 - 3.5 C 3.0 0.08 10Initial Grouping by Sensitivity or PrincipleColorimetric/turbidimetric 49 4.0 - 5.0 C 4.51 0.31 2.9 - 3.9 C 3.35 0.32 4.5 - 5.5 C 4.96 0.97 4.7 - 5.7 C 5.15 0.3 2.7 - 3.7 C 3.16 0.4 8Diluted ISE results 47 4.0 - 5.0 C 4.49 0.11 2.8 - 3.8 C 3.28 0.11 4.0 - 5.0 C 4.49 0.15 4.7 - 5.7 C 5.19 0.12 2.6 - 3.6 C 3.13 0.09 210Other 50 4.1 - 5.1 C 4.58 0.22 2.8 - 3.8 C 3.33 0.24 3.9 - 4.9 C 4.42 0.23 4.8 - 5.8 C 5.27 0.18 2.6 - 3.6 C 3.09 0.12 9Undiluted ISE results 48 4.1 - 5.1 C 4.56 0.11 2.8 - 3.8 C 3.33 0.13 3.9 - 4.9 C 4.44 0.13 4.8 - 5.8 C 5.3 0.15 2.6 - 3.6 C 3.14 0.08 262Total PopulationWhole Population 51 4.0 - 5.0 C 4.53 0.12 2.8 - 3.8 C 3.31 0.13 4.0 - 5.0 C 4.47 0.2 4.8 - 5.8 C 5.25 0.16 2.7 - 3.7 C 3.2 1.28 490

SodiumInitial Grouping by Reagent and InstrumentAVL & Roche/AVL 9100 1 138 - 146 C 141.5 0.5 122 - 130 C 126.3 0.8 151 - 159 C 154.5 0.5 145 - 153 C 149.0 0.7 123 - 131 C 126.5 0.9 4Abbott Architect & Abbott Architect c, ci, i 2 138 - 146 C 141.9 0.8 122 - 130 C 126.0 0.8 156 - 164 C 160.3 1.6 142 - 150 C 146.2 1.2 124 - 132 C 128.2 0.8 26Alfa Wassermann & Alfa Wasser Axcel/Alera 3 140 - 148 C 143.6 1.5 124 - 132 C 127.5 1.1 151 - 159 C 154.8 1.1 146 - 154 C 149.9 0.9 123 - 131 C 127.2 0.6 20Alfa Wassermann & Alfa Wasser Axcel/Alera 4 139 - 147 C 143.3 1.4 124 - 132 C 127.7 0.5 151 - 159 C 155.2 1.9 145 - 153 C 149.0 2.4 123 - 131 C 127.3 0.7 6Beckman Coulter & Beck Coult Unicel DXC 5 136 - 144 C 140.2 1.6 122 - 130 C 125.7 1.6 153 - 161 C 157.0 1.8 141 - 149 C 145.4 1.3 124 - 132 C 128.3 1.3 26Beckman Coulter & Beck Olym AU 400/600/5400 6 136 - 144 C 139.8 2.5 122 - 130 C 125.5 2.7 153 - 161 C 157.3 2.3 140 - 148 C 143.8 2.5 124 - 132 C 127.5 2.1 4Beckman Coulter & Beckman AU 480 7 136 - 144 C 139.8 1.5 121 - 129 C 125.0 2.4 153 - 161 C 157.3 1.5 140 - 148 C 144.0 1.6 124 - 132 C 128.3 1.8 4Beckman Olympus & Beck Olym AU 400/600/5400 8 135 - 143 C 139.0 1.5 121 - 129 C 124.7 1.2 153 - 161 C 157.0 1.8 139 - 147 C 143.1 1.2 124 - 132 C 127.5 1.2 43Beckman Olympus & Beck Olympus AU 2700 9 136 - 144 C 140.0 1.2 121 - 129 C 125.0 1.2 155 - 163 C 158.8 0.8 140 - 148 C 144.3 1.1 124 - 132 C 127.5 0.5 4Beckman Olympus & Beckman AU 480 10 134 - 142 C 138.4 1.4 120 - 128 C 123.8 0.8 152 - 160 C 156.3 1.8 139 - 147 C 142.5 1.3 123 - 131 C 126.8 1.0 15Carolina & Beckman Synchron CX3/7/9/L 11 136 - 144 C 140.0 3.1 122 - 130 C 125.8 1.8 152 - 160 C 156.1 2.6 141 - 149 C 144.8 2.4 125 - 133 C 129.1 1.4 9Horiba ABX & Horiba ABX 400 12 139 - 147 C 142.6 1.5 125 - 133 C 128.7 0.9 150 - 158 C 153.8 2.0 146 - 154 C 149.6 1.7 125 - 133 C 128.8 1.1 9Medica Easy RA & Medica Easy RA 13 139 - 147 C 142.9 5.0 127 - 135 C 130.6 1.2 153 - 161 C 156.9 1.4 148 - 156 C 151.9 1.5 127 - 135 C 130.6 1.3 8Medica EasyLyte & Medica EasyLyte series 14 139 - 147 C 143.4 1.2 127 - 135 C 131.3 0.4 151 - 159 C 154.6 1.9 147 - 155 C 151.2 0.7 126 - 134 C 130.0 0.6 5Ortho Vitros & Ortho Vitros 3600, 5600 15 143 - 151 C 147.3 2.3 129 - 137 C 132.5 1.7 160 - 168 C 164.1 2.8 152 - 160 C 155.9 2.7 130 - 138 C 133.5 2.8 8Ortho Vitros & Ortho Vitros 5,1 FS 16 142 - 150 C 145.5 1.6 127 - 135 C 130.8 1.5 158 - 166 C 162.2 1.7 149 - 157 C 152.8 2.3 127 - 135 C 130.8 1.1 6Ortho Vitros & Ortho Vitros not DT or ECi 17 143 - 151 C 147.4 1.8 129 - 137 C 133.2 2.0 160 - 168 C 164.4 2.8 150 - 158 C 153.6 8.6 129 - 137 C 133.4 2.1 33Roche Cobas dil ISE & Roche Cobas Integra 18 137 - 145 C 140.9 1.3 122 - 130 C 126.1 1.3 154 - 162 C 158.4 1.2 141 - 149 C 145.0 1.0 124 - 132 C 128.2 0.9 13Roche Cobas dil ISE & Roche e/c, 1XX, X000, Elec 19 137 - 145 C 141.3 1.7 122 - 130 C 126.4 1.3 156 - 164 C 159.8 1.6 143 - 151 C 146.7 1.4 126 - 134 C 129.5 1.9 22

series 19 137 - 145 C 141.3 1.7 122 - 130 C 126.4 1.3 156 - 164 C 159.8 1.6 143 - 151 C 146.7 1.4 126 - 134 C 129.5 1.9 22

Roche Cobas und ISE & Roche e/c, 1XX, X000, Elecseries 20 138 - 146 C 141.7 2.3 123 - 131 C 126.5 1.9 156 - 164 C 160.3 2.8 142 - 150 C 146.0 1.7 126 - 134 C 129.5 1.5 6

SDI Biomed & SDI CA-240, 480 21 138 - 146 C 142.3 2.1 125 - 133 C 128.8 1.3 150 - 158 C 154.4 2.8 146 - 154 C 150.0 1.7 125 - 133 C 129.0 1.2 10Siemens QuickLYTE IMT & Siemens Dim RxL, RxLMax 22 137 - 145 C 141.1 2.0 122 - 130 C 126.0 1.9 152 - 160 C 155.8 2.1 140 - 148 C 143.9 1.8 125 - 133 C 129.4 2.9 10

Siemens QuickLYTE IMT & Siemens Dimension 23 139 - 147 C 142.6 1.2 124 - 132 C 127.8 1.1 156 - 164 C 159.6 1.2 143 - 151 C 146.6 1.0 126 - 134 C 130.1 0.6 8Siemens QuickLYTE IMT & Siemens Dimension EXL 24 138 - 146 C 142.4 1.0 124 - 132 C 127.9 0.9 155 - 163 C 159.4 1.0 142 - 150 C 146.0 1.1 126 - 134 C 130.4 1.1 59Siemens QuickLYTE IMT & Siemens DimensionXpand 25 139 - 147 C 142.8 1.6 124 - 132 C 128.0 1.3 156 - 164 C 159.8 1.7 143 - 151 C 146.5 1.6 126 - 134 C 130.4 1.3 42

Siemens dil ISE & Siemens Advia 26 139 - 147 C 142.7 1.2 124 - 132 C 128.0 0.5 156 - 164 C 160.0 1.1 143 - 151 C 147.0 0.9 126 - 134 C 130.1 1.1 7Vital Diagnostic Eon & Vital Diagnostic Eon 27 140 - 148 C 144.0 1.0 127 - 135 C 130.5 2.5 152 - 160 C 156.0 0.8 144 - 152 C 148.0 4.1 124 - 132 C 128.0 2.1 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 28 136 - 144 C 140.2 2.2 122 - 130 C 125.6 1.5 153 - 161 C 156.9 2.6 141 - 149 C 145.3 2.3 125 - 133 C 128.7 2.6 10Initial Grouping by ReagentAVL 29 137 - 145 C 141.4 0.5 122 - 130 C 126.4 0.8 150 - 158 C 154.2 0.7 145 - 153 C 149.0 0.6 122 - 130 C 126.4 0.8 5Abbott Architect 30 138 - 146 C 141.9 0.8 122 - 130 C 126.0 0.8 156 - 164 C 160.3 1.6 142 - 150 C 146.2 1.2 124 - 132 C 128.2 0.8 26Alfa Wassermann 32 139 - 147 C 143.4 1.3 124 - 132 C 127.7 0.5 151 - 159 C 155.0 1.8 145 - 153 C 149.1 2.3 123 - 131 C 127.3 0.7 7Alfa Wassermann 31 140 - 148 C 143.5 1.5 124 - 132 C 127.5 1.1 151 - 159 C 154.8 1.1 146 - 154 C 149.8 0.9 123 - 131 C 127.2 0.6 21Beckman Coulter 33 136 - 144 C 140.1 1.7 122 - 130 C 125.6 1.9 153 - 161 C 157.1 1.8 141 - 149 C 145.1 1.6 124 - 132 C 128.2 1.5 36Beckman Olympus 34 135 - 143 C 138.9 1.5 121 - 129 C 124.5 1.2 153 - 161 C 156.9 1.9 139 - 147 C 143.0 1.3 123 - 131 C 127.3 1.2 64Carolina 35 136 - 144 C 140.1 2.7 122 - 130 C 126.3 2.1 152 - 160 C 156.3 4.2 142 - 150 C 145.5 2.8 125 - 133 C 128.7 1.5 15Diamond Diagnostics 36 136 - 144 C 139.8 2.0 122 - 130 C 125.6 1.4 153 - 161 C 157.0 2.7 140 - 148 C 144.2 2.6 124 - 132 C 127.6 0.8 5Horiba ABX 37 139 - 147 C 142.6 1.5 125 - 133 C 128.7 0.9 150 - 158 C 153.8 2.0 146 - 154 C 149.6 1.7 125 - 133 C 128.8 1.1 9Medica Easy RA 38 139 - 147 C 142.9 5.0 127 - 135 C 130.6 1.2 153 - 161 C 156.9 1.4 148 - 156 C 151.9 1.5 127 - 135 C 130.6 1.3 8Medica EasyLyte 39 139 - 147 C 143.4 1.8 127 - 135 C 130.8 1.5 151 - 159 C 155.1 2.6 147 - 155 C 150.7 1.3 126 - 134 C 130.2 1.9 9Ortho Vitros 40 143 - 151 C 147.2 1.9 129 - 137 C 132.9 2.0 160 - 168 C 164.1 2.8 150 - 158 C 154.1 7.1 129 - 137 C 133.1 2.3 51Pointe Scientific 41 136 - 144 C 140.2 5.1 122 - 130 C 125.8 4.4 151 - 159 C 154.6 4.9 140 - 148 C 144.2 6.3 122 - 130 C 126.4 3.4 5Roche Cobas dil ISE 42 137 - 145 C 141.3 1.6 122 - 130 C 126.3 1.3 155 - 163 C 159.4 1.6 142 - 150 C 146.2 1.5 125 - 133 C 129.0 1.6 38Roche Cobas und ISE 43 138 - 146 C 142.3 2.0 123 - 131 C 127.0 2.0 155 - 163 C 158.6 3.2 143 - 151 C 146.6 2.0 125 - 133 C 129.0 1.7 11SDI Biomed 44 138 - 146 C 142.4 1.9 125 - 133 C 128.8 1.5 151 - 159 C 154.8 2.7 146 - 154 C 150.2 1.6 125 - 133 C 129.1 1.3 12Siemens QuickLYTE IMT 45 139 - 147 C 142.5 1.4 124 - 132 C 127.8 1.3 155 - 163 C 159.2 1.7 142 - 150 C 146.1 1.5 126 - 134 C 130.3 1.4 120Siemens dil ISE 46 139 - 147 C 142.7 1.2 124 - 132 C 128.0 0.5 156 - 164 C 160.0 1.1 143 - 151 C 147.0 0.9 126 - 134 C 130.1 1.1 7Vital Diagnostic Eon 47 140 - 148 C 144.0 1.0 127 - 135 C 130.5 2.5 152 - 160 C 156.0 0.8 144 - 152 C 148.0 4.1 124 - 132 C 128.0 2.1 4Vital Diagnostics Envoy 48 136 - 144 C 140.2 2.2 122 - 130 C 125.6 1.5 153 - 161 C 156.9 2.6 141 - 149 C 145.3 2.3 125 - 133 C 128.7 2.6 10Initial Grouping by Sensitivity or PrincipleColorimetric 51 138 - 146 C 141.9 5.1 123 - 131 C 126.9 4.7 153 - 161 C 156.9 5.0 141 - 149 C 145.3 5.5 124 - 132 C 128.1 5.2 8Diluted ISE 49 136 - 144 C 140.4 2.1 122 - 130 C 125.8 1.9 154 - 162 C 158.0 2.5 141 - 149 C 145.0 2.4 124 - 132 C 128.3 1.6 209Other 52 139 - 147 C 143.2 6.0 126 - 134 C 129.5 10.2 152 - 160 C 155.6 5.2 145 - 153 C 148.6 8.2 125 - 133 C 128.6 5.1 10Undiluted ISE 50 140 - 148 C 143.5 2.7 125 - 133 C 128.9 2.6 155 - 163 C 159.0 3.8 145 - 153 C 148.8 4.7 126 - 134 C 130.3 2.4 260Total PopulationWhole Population 53 138 - 146 C 142.1 3.2 124 - 132 C 127.6 3.5 154 - 162 C 158.4 4.1 143 - 151 C 147.1 4.4 125 - 133 C 129.4 2.6 488

Protein, TotalInitial Grouping by Reagent and InstrumentAbbott & Abbott Architect c, ci, i 1 5.5 - 6.7 P 6.11 0.08 5.7 - 6.9 P 6.28 0.11 5.0 - 6.1 P 5.55 0.12 6.9 - 8.4 P 7.64 0.12 4.2 - 5.2 P 4.7 0.08 28Alfa Wassermann & Alfa Wasser Axcel/Alera 2 5.5 - 6.8 P 6.14 0.17 5.7 - 6.9 P 6.31 0.16 5.0 - 6.2 P 5.61 0.17 6.9 - 8.4 P 7.63 0.19 4.3 - 5.3 P 4.79 0.13 25All refractometers & All other refractometers 3 6.8 - 8.4 P 7.61 0.18 6.9 - 8.4 P 7.68 0.12 6.0 - 7.3 P 6.65 0.11 8.4 - 10.3 P 9.36 0.14 5.3 - 6.5 P 5.94 0.13 310All refractometers & Reichert TS Meter-DSP 4 6.8 - 8.3 P 7.58 0.11 6.9 - 8.5 P 7.69 0.13 6.0 - 7.3 P 6.67 0.1 8.4 - 10.3 P 9.37 0.12 5.4 - 6.5 P 5.95 0.1 43Beckman Olympus & Beck Olym AU 400/600/5400 5 5.4 - 6.6 P 6.04 0.14 5.6 - 6.8 P 6.21 0.14 5.0 - 6.2 P 5.61 0.15 6.8 - 8.3 P 7.52 0.18 4.2 - 5.2 P 4.69 0.12 38Beckman Olympus & Beck Olympus AU 2700 6 5.5 - 6.7 P 6.1 0.07 5.5 - 6.8 P 6.15 0.05 5.1 - 6.2 P 5.65 0.05 6.8 - 8.3 P 7.5 0.12 4.2 - 5.1 P 4.68 0.08 4Beckman Olympus & Beckman AU 480 7 5.5 - 6.7 P 6.07 0.17 5.6 - 6.8 P 6.22 0.17 5.1 - 6.2 P 5.62 0.16 6.8 - 8.3 P 7.54 0.23 4.2 - 5.2 P 4.71 0.13 17Beckman rate & Beck Coult Unicel DXC 8 5.5 - 6.7 P 6.12 0.2 5.6 - 6.9 P 6.23 0.23 5.3 - 6.4 P 5.85 0.14 6.9 - 8.4 P 7.65 0.21 4.3 - 5.2 P 4.73 0.11 6Beckman unc & Beck Coult Unicel DXC 9 5.4 - 6.6 P 6.04 0.11 5.6 - 6.8 P 6.21 0.1 5.2 - 6.4 P 5.82 0.08 6.8 - 8.3 P 7.56 0.12 4.2 - 5.1 P 4.68 0.09 19Carolina & Beckman Synchron CX3/7/9/L 10 5.3 - 6.4 P 5.84 0.28 5.4 - 6.6 P 5.99 0.29 5.0 - 6.2 P 5.61 0.25 6.6 - 8.1 P 7.37 0.34 4.0 - 4.9 P 4.49 0.2 8Horiba ABX & Horiba ABX 400 11 5.4 - 6.6 P 6.02 0.11 5.6 - 6.9 P 6.23 0.22 4.9 - 6.0 P 5.48 0.11 6.8 - 8.3 P 7.52 0.08 4.2 - 5.2 P 4.69 0.11 9Ortho Vitros & Ortho Vitros 3600, 5600 12 5.4 - 6.6 P 5.99 0.21 5.7 - 6.9 P 6.29 0.13 5.0 - 6.1 P 5.5 0.11 6.7 - 8.2 P 7.41 0.23 4.3 - 5.3 P 4.83 0.12 9Ortho Vitros & Ortho Vitros 5,1 FS 13 5.3 - 6.5 P 5.9 0.15 5.6 - 6.8 P 6.18 0.2 4.8 - 5.9 P 5.38 0.21 6.5 - 7.9 P 7.18 0.21 4.2 - 5.2 P 4.72 0.12 6Ortho Vitros & Ortho Vitros not DT or ECi 14 5.3 - 6.5 P 5.93 0.1 5.6 - 6.8 P 6.21 0.11 4.9 - 6.0 P 5.43 0.09 6.6 - 8.0 P 7.3 0.12 4.3 - 5.3 P 4.78 0.08 32Reichert TS Meter-DSP & Reichert TS Meter-DSP 15 6.9 - 8.4 P 7.62 0.13 6.9 - 8.5 P 7.69 0.1 6.0 - 7.3 P 6.68 0.14 8.4 - 10.3 P 9.38 0.12 5.4 - 6.5 P 5.95 0.1 104Roche Cobas & Roche Cobas Integra 16 5.4 - 6.6 P 6.03 0.17 5.6 - 6.9 P 6.24 0.14 5.1 - 6.2 P 5.64 0.12 6.8 - 8.3 P 7.5 0.16 4.3 - 5.2 P 4.73 0.08 14Roche Cobas & Roche e/c, 1XX, X000, Elec series 17 5.5 - 6.8 P 6.16 0.12 5.7 - 7.0 P 6.32 0.1 5.2 - 6.3 P 5.74 0.1 6.8 - 8.4 P 7.61 0.16 4.3 - 5.3 P 4.81 0.1 28SDI Biomed & SDI CA-240, 480 18 5.7 - 7.0 P 6.36 0.14 5.9 - 7.3 P 6.61 0.24 5.1 - 6.2 P 5.62 0.17 7.0 - 8.6 P 7.78 0.16 4.4 - 5.4 P 4.87 0.1 11Sekisui & Beck Olym AU 400/600/5400 19 5.5 - 6.7 P 6.06 0.17 5.6 - 6.8 P 6.2 0.23 5.0 - 6.2 P 5.6 0.23 6.8 - 8.3 P 7.52 0.24 4.3 - 5.3 P 4.78 0.26 5

Siemens Dimension & Siemens Dim RxL, RxL Max 20 5.7 - 6.9 P 6.31 0.11 5.8 - 7.1 P 6.42 0.11 5.3 - 6.4 P 5.84 0.08 7.0 - 8.6 P 7.83 0.16 4.4 - 5.4 P 4.9 0.07 9Siemens Dimension & Siemens Dimension 21 5.6 - 6.8 P 6.17 0.28 5.7 - 7.0 P 6.36 0.33 5.2 - 6.4 P 5.79 0.28 7.0 - 8.5 P 7.73 0.41 4.3 - 5.3 P 4.81 0.24 7Siemens Dimension & Siemens Dimension EXL 22 5.7 - 7.0 P 6.32 0.13 5.8 - 7.1 P 6.49 0.14 5.3 - 6.5 P 5.89 0.12 7.1 - 8.6 P 7.86 0.17 4.4 - 5.4 P 4.93 0.13 54Siemens Dimension & Siemens Dimension Xpand 23 5.7 - 6.9 P 6.28 0.11 5.8 - 7.1 P 6.45 0.12 5.3 - 6.4 P 5.85 0.1 7.0 - 8.6 P 7.83 0.15 4.4 - 5.4 P 4.91 0.08 37Siemens biuret based & Siemens Advia 24 5.6 - 6.8 P 6.21 0.15 5.8 - 7.0 P 6.4 0.13 5.2 - 6.3 P 5.75 0.12 6.9 - 8.5 P 7.71 0.18 4.1 - 5.0 P 4.56 0.71 8Siemens biuret based & Siemens Dimension EXL 25 5.7 - 7.0 P 6.35 0.08 5.9 - 7.2 P 6.5 0.08 5.3 - 6.5 P 5.88 0.11 7.1 - 8.7 P 7.87 0.14 4.4 - 5.4 P 4.92 0.07 6Siemens biuret based & Siemens Dimension Xpand 26 5.8 - 7.1 P 6.46 0.05 5.9 - 7.2 P 6.54 0.05 5.4 - 6.6 P 5.96 0.05 7.2 - 8.8 P 7.98 0.07 4.5 - 5.5 P 4.98 0.04 5Synermed & Synermed IR500 27 5.7 - 7.0 P 6.32 0.34 5.9 - 7.2 P 6.56 0.49 5.2 - 6.4 P 5.8 0.34 7.0 - 8.6 P 7.78 0.42 4.4 - 5.4 P 4.88 0.35 5Vital Diagnostic & Vital Diagnostic Eon 28 5.6 - 6.8 P 6.2 0.29 5.8 - 7.0 P 6.4 0.27 5.2 - 6.3 P 5.75 0.34 6.8 - 8.4 P 7.6 0.59 4.4 - 5.4 P 4.88 0.18 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 29 5.7 - 7.0 P 6.34 0.27 5.8 - 7.1 P 6.46 0.24 5.2 - 6.3 P 5.76 0.26 7.1 - 8.7 P 7.93 0.32 4.5 - 5.5 P 4.96 0.19 8Initial Grouping by ReagentAbbott 30 5.5 - 6.7 P 6.11 0.08 5.7 - 6.9 P 6.28 0.11 5.0 - 6.1 P 5.55 0.12 6.9 - 8.4 P 7.64 0.12 4.2 - 5.2 P 4.7 0.08 30Alfa Wassermann 31 5.5 - 6.8 P 6.14 0.17 5.7 - 6.9 P 6.3 0.16 5.0 - 6.2 P 5.61 0.17 6.9 - 8.4 P 7.63 0.19 4.3 - 5.3 P 4.79 0.12 27All refractometers 32 6.8 - 8.4 P 7.61 0.17 6.9 - 8.4 P 7.68 0.12 6.0 - 7.3 P 6.65 0.11 8.4 - 10.3 P 9.36 0.14 5.3 - 6.5 P 5.94 0.13 356Beckman Olympus 33 5.5 - 6.7 P 6.06 0.14 5.6 - 6.8 P 6.22 0.15 5.1 - 6.2 P 5.62 0.15 6.8 - 8.3 P 7.53 0.19 4.2 - 5.2 P 4.7 0.12 61Beckman rate 34 5.5 - 6.7 P 6.13 0.17 5.6 - 6.9 P 6.24 0.2 5.3 - 6.4 P 5.85 0.12 6.9 - 8.4 P 7.64 0.19 4.3 - 5.2 P 4.73 0.1 8Beckman unc 35 5.4 - 6.6 P 6.04 0.11 5.6 - 6.8 P 6.2 0.1 5.2 - 6.4 P 5.8 0.11 6.8 - 8.3 P 7.56 0.12 4.2 - 5.1 P 4.68 0.09 21Carolina 36 5.3 - 6.5 P 5.92 0.22 5.5 - 6.7 P 6.06 0.25 5.0 - 6.2 P 5.61 0.19 6.7 - 8.2 P 7.44 0.28 4.1 - 5.0 P 4.59 0.19 19Horiba ABX 37 5.4 - 6.6 P 6.02 0.11 5.6 - 6.9 P 6.23 0.22 4.9 - 6.0 P 5.48 0.11 6.8 - 8.3 P 7.52 0.08 4.2 - 5.2 P 4.69 0.11 9Ortho Vitros 38 5.3 - 6.5 P 5.94 0.14 5.6 - 6.9 P 6.23 0.14 4.9 - 6.0 P 5.45 0.12 6.6 - 8.0 P 7.31 0.17 4.3 - 5.3 P 4.78 0.1 51Pointe Scientific 39 5.6 - 6.9 P 6.27 0.29 5.8 - 7.1 P 6.49 0.22 5.3 - 6.4 P 5.85 0.25 7.1 - 8.7 P 7.87 0.3 4.4 - 5.4 P 4.89 0.2 10Reichert TS Meter-DSP 40 6.9 - 8.4 P 7.62 0.13 6.9 - 8.5 P 7.69 0.1 6.0 - 7.3 P 6.68 0.14 8.4 - 10.3 P 9.38 0.12 5.4 - 6.5 P 5.95 0.1 104Roche Cobas 41 5.5 - 6.7 P 6.12 0.16 5.7 - 6.9 P 6.29 0.13 5.1 - 6.3 P 5.71 0.12 6.8 - 8.3 P 7.58 0.17 4.3 - 5.3 P 4.78 0.11 46SDI Biomed 42 5.7 - 7.0 P 6.34 0.16 5.9 - 7.2 P 6.56 0.26 5.0 - 6.1 P 5.59 0.18 7.0 - 8.5 P 7.75 0.16 4.3 - 5.3 P 4.83 0.12 13Sekisui 43 5.5 - 6.7 P 6.06 0.17 5.6 - 6.8 P 6.22 0.19 5.0 - 6.1 P 5.54 0.25 6.8 - 8.3 P 7.51 0.21 4.3 - 5.2 P 4.73 0.2 10Siemens Dimension 44 5.7 - 6.9 P 6.3 0.14 5.8 - 7.1 P 6.46 0.16 5.3 - 6.5 P 5.87 0.13 7.1 - 8.6 P 7.84 0.19 4.4 - 5.4 P 4.91 0.13 107Siemens biuret based 45 5.7 - 6.9 P 6.31 0.15 5.8 - 7.1 P 6.47 0.11 5.3 - 6.4 P 5.85 0.13 7.1 - 8.6 P 7.84 0.17 4.3 - 5.3 P 4.8 0.48 21Synermed 46 5.7 - 6.9 P 6.3 0.32 5.9 - 7.2 P 6.55 0.45 5.2 - 6.4 P 5.82 0.31 7.0 - 8.6 P 7.8 0.38 4.4 - 5.4 P 4.88 0.32 6Vital Diagnostic 47 5.7 - 6.9 P 6.3 0.33 5.9 - 7.2 P 6.5 0.32 5.2 - 6.4 P 5.8 0.32 7.0 - 8.5 P 7.76 0.62 4.4 - 5.4 P 4.92 0.18 5Vital Diagnostics Envoy 48 5.7 - 7.0 P 6.34 0.27 5.8 - 7.1 P 6.46 0.24 5.2 - 6.3 P 5.76 0.26 7.1 - 8.7 P 7.93 0.32 4.5 - 5.5 P 4.96 0.19 8Initial Grouping by Sensitivity or PrincipleAll refractometer users 50 6.8 - 8.4 P 7.61 0.17 6.9 - 8.4 P 7.68 0.12 6.0 - 7.3 P 6.65 0.11 8.4 - 10.3 P 9.36 0.14 5.3 - 6.5 P 5.94 0.13 356Bichromatic-no sam blanks 49 5.5 - 6.8 P 6.14 0.2 5.7 - 6.9 P 6.31 0.21 5.1 - 6.2 P 5.65 0.2 6.9 - 8.4 P 7.64 0.25 4.3 - 5.2 P 4.75 0.23 173Bichromatic-sample blanked 54 5.7 - 6.9 P 6.3 0.14 5.8 - 7.1 P 6.46 0.16 5.3 - 6.5 P 5.87 0.13 7.1 - 8.6 P 7.84 0.19 4.4 - 5.4 P 4.91 0.13 108Just sample blanked 55 5.5 - 6.7 P 6.12 0.16 5.7 - 6.9 P 6.29 0.13 5.1 - 6.3 P 5.71 0.12 6.8 - 8.3 P 7.58 0.17 4.3 - 5.3 P 4.78 0.11 46Rate 51 5.4 - 6.6 P 5.98 0.23 5.5 - 6.7 P 6.11 0.25 5.1 - 6.2 P 5.68 0.21 6.8 - 8.3 P 7.5 0.27 4.2 - 5.1 P 4.63 0.18 27Other 53 6.4 - 7.9 P 7.14 0.81 6.5 - 7.9 P 7.19 0.71 5.8 - 7.0 P 6.39 0.61 7.9 - 9.7 P 8.79 0.93 5.0 - 6.1 P 5.56 0.62 33Uncorrected 52 5.4 - 6.7 P 6.05 0.22 5.7 - 6.9 P 6.29 0.22 5.0 - 6.1 P 5.58 0.23 6.7 - 8.2 P 7.49 0.28 4.3 - 5.2 P 4.77 0.15 111Total PopulationWhole Population 56 6.2 - 7.6 P 6.9 0.88 6.3 - 7.7 P 7.03 0.98 5.6 - 6.8 P 6.21 1.12 7.7 - 9.4 P 8.55 1.6 4.9 - 6.0 P 5.44 1.76 960

TriglyceridesInitial Grouping by Reagent and InstrumentAbbott & Abbott Architect c, ci, i 1 102 - 169 P 135.5 2.9 97 - 162 P 129.5 2.9 20 - 33 P 26.5 1.3 132 - 221 P 176.6 3.3 68 - 114 P 91.3 2.2 26Alfa Wassermann & Alfa Wasser Axcel/Alera 2 104 - 174 P 139.3 3.8 98 - 164 P 131.0 3.4 24 - 40 P 32.3 2.0 134 - 223 P 178.4 6.6 72 - 120 P 96.0 2.7 25Beckman GPO glycerol-unc & Beck Coult UnicelDXC 3 111 - 184 P 147.5 3.8 106 - 176 P 140.8 4.8 24 - 40 P 32.2 2.8 145 - 242 P 193.3 5.3 74 - 124 P 99.0 3.4 21

Beckman Olympus & Beck Olym AU 400/600/5400 4 105 - 175 P 140.1 4.6 100 - 166 P 132.7 4.9 20 - 33 P 26.8 1.3 137 - 229 P 183.1 7.1 70 - 116 P 92.9 3.3 33Beckman Olympus & Beck Olympus AU 2700 5 105 - 174 P 139.5 2.3 98 - 163 P 130.0 1.4 20 - 33 P 26.3 0.8 133 - 222 P 177.5 2.3 68 - 114 P 91.0 1.2 4Beckman Olympus & Beckman AU 480 6 104 - 174 P 138.9 5.8 98 - 163 P 130.4 5.7 20 - 33 P 26.8 1.4 134 - 224 P 179.3 8.5 70 - 116 P 92.7 3.3 14Carolina & Beckman Synchron CX3/7/9/L 7 113 - 188 P 150.5 14.4 103 - 172 P 137.5 9.0 22 - 37 P 29.9 3.3 142 - 237 P 189.3 12.3 71 - 118 P 94.1 8.1 8Horiba ABX & Horiba ABX 400 8 106 - 176 P 140.8 5.1 98 - 164 P 131.2 7.7 21 - 35 P 28.0 1.9 136 - 226 P 180.7 6.1 71 - 118 P 94.3 4.2 6Ortho Vitros & Ortho Vitros 3600, 5600 9 108 - 180 P 144.1 5.4 104 - 174 P 139.1 5.7 21 - 34 P 27.5 1.8 149 - 248 P 198.3 5.3 71 - 119 P 95.1 4.0 8Ortho Vitros & Ortho Vitros 5,1 FS 10 110 - 183 P 146.5 5.4 103 - 172 P 137.8 5.4 21 - 35 P 27.7 1.7 147 - 246 P 196.5 7.0 70 - 117 P 93.8 4.1 6Ortho Vitros & Ortho Vitros not DT or ECi 11 111 - 186 P 148.5 7.6 106 - 176 P 140.9 5.3 21 - 35 P 28.0 1.3 148 - 247 P 197.3 8.5 72 - 120 P 96.3 4.3 28Pointe Scientific & Other spectrophotometers 12 97 - 161 P 128.7 6.7 87 - 146 P 116.5 6.0 18 - 30 P 24.0 1.5 126 - 210 P 167.7 7.8 63 - 106 P 84.5 5.1 6Pointe Scientific & Pointe Sci 180/Sterl 2000 13 85 - 142 P 113.8 10.8 74 - 123 P 98.2 13.8 16 - 27 P 21.9 3.9 112 - 187 P 149.6 13.8 52 - 87 P 69.8 9.6 9Roche Cobas & Roche Cobas Integra 14 104 - 173 P 138.3 2.3 97 - 161 P 129.1 2.1 22 - 36 P 28.9 0.9 134 - 224 P 178.9 3.7 70 - 117 P 93.5 1.3 12Roche Cobas & Roche e/c, 1XX, X000, Elec series 15 106 - 177 P 141.3 3.4 100 - 167 P 133.4 3.3 22 - 37 P 29.3 1.2 136 - 227 P 181.7 6.0 72 - 120 P 96.2 2.8 29SDI Biomed & SDI CA-240, 480 16 112 - 187 P 149.3 7.2 98 - 163 P 130.3 7.5 21 - 35 P 27.6 1.0 145 - 242 P 193.4 5.9 72 - 120 P 96.1 4.2 8Sekisui & Beck Olym AU 400/600/5400 17 103 - 172 P 137.6 5.2 94 - 156 P 124.8 6.0 21 - 35 P 27.8 1.7 132 - 220 P 176.2 6.2 68 - 113 P 90.0 3.9 5Sekisui & Roche Cobas Mira series 18 107 - 179 P 143.2 5.2 93 - 155 P 123.8 4.7 19 - 32 P 25.8 2.2 138 - 230 P 183.8 7.1 69 - 116 P 92.6 4.3 5

Siemens Advia & Siemens Advia 19 107 - 178 P 142.2 2.2 101 - 169 P 135.0 1.7 21 - 36 P 28.4 1.0 138 - 229 P 183.4 2.4 72 - 119 P 95.4 1.0 5Siemens Dimension & Siemens Dim RxL, RxL Max 20 101 - 169 P 135.3 6.9 97 - 161 P 129.0 6.6 15 - 25 P 20.2 1.4 134 - 223 P 178.5 7.8 67 - 112 P 89.5 3.6 10Siemens Dimension & Siemens Dimension 21 103 - 171 P 137.0 3.4 98 - 163 P 130.8 2.5 16 - 27 P 21.8 1.1 134 - 224 P 179.0 1.6 67 - 112 P 89.5 1.1 4Siemens Dimension & Siemens Dimension EXL 22 103 - 171 P 136.7 3.7 98 - 163 P 130.5 3.7 15 - 26 P 20.4 1.7 135 - 226 P 180.5 3.7 67 - 112 P 89.9 3.3 51Siemens Dimension & Siemens Dimension Xpand 23 103 - 172 P 137.3 2.6 98 - 163 P 130.6 2.8 16 - 26 P 20.9 1.5 135 - 226 P 180.5 3.7 68 - 113 P 90.6 2.5 37Siemens Express & Siemens Dimension EXL 24 102 - 170 P 135.9 2.8 98 - 164 P 131.0 5.7 16 - 26 P 20.9 1.7 134 - 223 P 178.5 3.4 68 - 113 P 90.0 3.4 8Siemens Express & Siemens Dimension Xpand 25 100 - 167 P 133.8 2.5 97 - 161 P 128.8 3.8 17 - 29 P 23.0 4.1 134 - 223 P 178.3 1.6 66 - 110 P 88.3 3.0 4Sterling Diagnostic & Other spectrophotometers 26 109 - 182 P 145.5 4.3 100 - 166 P 132.8 5.6 20 - 33 P 26.7 2.8 144 - 240 P 192.4 9.4 69 - 116 P 92.6 2.6 11Sterling Diagnostic & Pointe Sci 180/Sterl 2000 27 112 - 187 P 149.3 5.8 101 - 168 P 134.2 5.0 20 - 33 P 26.7 4.0 146 - 244 P 195.2 7.6 70 - 117 P 93.3 5.4 6Synermed & Synermed IR500 28 122 - 203 P 162.8 3.8 116 - 194 P 155.0 3.1 26 - 43 P 34.7 1.7 154 - 257 P 205.8 3.6 83 - 138 P 110.5 7.4 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 29 104 - 174 P 139.1 2.8 99 - 165 P 131.7 3.4 22 - 37 P 29.6 2.8 140 - 233 P 186.1 11.2 64 - 106 P 84.9 30.2 9Initial Grouping by ReagentAbbott 30 102 - 169 P 135.5 2.9 97 - 162 P 129.5 2.9 20 - 33 P 26.5 1.3 132 - 221 P 176.6 3.3 68 - 114 P 91.3 2.2 26Alfa Wassermann 31 104 - 174 P 139.1 3.8 98 - 163 P 130.7 3.4 24 - 40 P 32.3 2.0 133 - 223 P 178.0 6.5 72 - 120 P 96.1 2.9 27Beckman GPO glycerol-unc 32 110 - 184 P 147.1 4.5 105 - 175 P 140.1 5.8 24 - 40 P 32.2 2.8 145 - 241 P 193.2 6.9 74 - 123 P 98.7 3.9 26Beckman Olympus 33 105 - 175 P 139.8 4.8 99 - 165 P 131.8 5.0 20 - 33 P 26.8 1.3 136 - 227 P 181.6 7.4 70 - 116 P 92.8 3.2 53Carolina 34 108 - 180 P 143.6 13.8 95 - 158 P 126.3 16.7 21 - 34 P 27.4 5.1 134 - 223 P 178.7 18.6 67 - 111 P 88.9 11.1 19Horiba ABX 35 106 - 176 P 140.8 5.1 98 - 164 P 131.2 7.7 21 - 35 P 28.0 1.9 136 - 226 P 180.7 6.1 71 - 118 P 94.3 4.2 6Ortho Vitros 36 111 - 185 P 147.7 7.9 105 - 175 P 140.4 6.2 21 - 35 P 27.8 1.5 148 - 247 P 197.8 8.4 72 - 120 P 95.9 5.0 45Pointe Scientific 37 94 - 156 P 125.2 14.5 84 - 141 P 112.4 16.3 18 - 30 P 24.0 3.1 122 - 204 P 163.2 18.2 60 - 100 P 80.1 11.4 27Roche Cobas 38 106 - 176 P 140.7 3.6 99 - 165 P 132.3 4.0 22 - 37 P 29.2 1.1 136 - 226 P 181.0 5.8 72 - 119 P 95.5 2.9 45SDI Biomed 39 110 - 183 P 146.6 9.1 98 - 163 P 130.0 6.7 21 - 34 P 27.4 1.2 144 - 240 P 191.8 6.3 71 - 119 P 95.1 4.3 10Sekisui 40 106 - 176 P 140.7 6.8 94 - 157 P 125.3 7.6 20 - 34 P 26.9 2.3 135 - 226 P 180.4 9.2 69 - 114 P 91.4 5.4 12Siemens Advia 41 107 - 178 P 142.2 2.2 101 - 169 P 135.0 1.7 21 - 36 P 28.4 1.0 138 - 229 P 183.4 2.4 72 - 119 P 95.4 1.0 5Siemens Dimension 42 103 - 171 P 137.0 4.0 98 - 163 P 130.5 3.9 15 - 26 P 20.6 1.6 135 - 226 P 180.4 4.4 68 - 113 P 90.1 3.4 104Siemens Express 43 102 - 170 P 135.7 2.9 98 - 163 P 130.5 4.9 16 - 27 P 21.5 2.6 134 - 224 P 178.9 3.2 67 - 112 P 89.5 3.2 15Sterling Diagnostic 44 110 - 184 P 147.0 5.1 100 - 166 P 132.9 5.5 20 - 33 P 26.3 3.6 145 - 242 P 193.6 8.7 70 - 116 P 93.0 3.8 18Synermed 45 120 - 200 P 159.8 6.8 114 - 191 P 152.4 5.9 26 - 43 P 34.3 1.6 153 - 255 P 204.0 4.7 82 - 136 P 108.8 7.5 5Vital Diagnostic 46 102 - 170 P 136.0 5.7 96 - 160 P 128.0 6.8 20 - 34 P 27.2 2.0 134 - 223 P 178.4 6.1 71 - 119 P 94.9 9.9 7Vital Diagnostics Envoy 47 104 - 174 P 139.1 2.8 99 - 165 P 131.7 3.4 22 - 37 P 29.6 2.8 140 - 233 P 186.1 11.2 72 - 119 P 95.5 3.3 9Initial Grouping by Sensitivity or PrincipleGly-cor/visible/GPO-based 50 105 - 175 P 140.3 7.1 99 - 165 P 132.1 9.8 22 - 37 P 29.2 3.5 136 - 227 P 181.2 9.7 71 - 118 P 94.1 5.0 9Gly-unc/340nm/GDH-based 52 102 - 170 P 135.7 2.9 98 - 163 P 130.5 4.9 16 - 27 P 21.5 2.6 134 - 224 P 178.9 3.2 67 - 112 P 89.5 3.2 15Gly-unc/visible/GPO-based 48 105 - 175 P 139.9 9.2 98 - 164 P 131.1 10.0 19 - 33 P 26.0 4.1 137 - 229 P 182.9 12.4 69 - 115 P 92.2 7.0 421Gly-unc/visible/INT-based 49 104 - 173 P 138.8 4.1 98 - 163 P 130.7 3.4 24 - 40 P 32.1 2.3 133 - 222 P 177.7 6.6 72 - 120 P 95.7 3.4 28Other 51 108 - 181 P 144.5 11.0 94 - 156 P 125.1 35.5 22 - 37 P 29.9 3.8 130 - 217 P 173.4 45.9 65 - 109 P 87.0 26.5 8Total PopulationWhole Population 53 107 - 178 P 142.5 60.7 98 - 164 P 130.9 12.2 20 - 33 P 26.7 6.3 137 - 228 P 182.1 15.3 69 - 115 P 92.3 8.9 482

Urea Nitrogen (BUN)Initial Grouping by Reagent and InstrumentAbbott & Abbott Architect c, ci, i 1 26 - 31 P 28.2 0.7 39 - 47 P 43.1 0.9 10 - 14 C 12.0 0.4 28 - 34 P 31.0 0.5 31 - 37 P 34.0 0.8 26Alfa Wassermann & Alfa Wasser Axcel/Alera 2 26 - 31 P 28.5 1.4 39 - 47 P 43.4 1.9 10 - 14 C 12.3 0.9 28 - 34 P 30.9 1.5 31 - 38 P 34.5 1.8 29Beckman GLDH-rate & Beck Coult Unicel DXC 3 26 - 31 P 28.6 0.7 39 - 47 P 43.3 1.0 11 - 15 C 13.0 0.0 29 - 35 P 31.8 0.7 31 - 37 P 34.3 0.6 23Beckman Olympus & Beck Olym AU 400/600/5400 4 26 - 31 P 28.1 1.1 39 - 46 P 42.5 1.4 10 - 14 C 11.9 0.6 28 - 33 P 30.6 1.1 31 - 37 P 33.7 1.2 37Beckman Olympus & Beck Olympus AU 2700 5 25 - 30 P 27.8 0.4 38 - 46 P 41.8 0.4 10 - 14 C 11.8 0.4 27 - 33 P 30.0 0.0 30 - 36 P 32.8 0.4 4Beckman Olympus & Beckman AU 480 6 26 - 31 P 28.2 1.0 39 - 47 P 43.4 1.0 10 - 14 C 12.1 0.3 28 - 34 P 31.1 0.7 31 - 37 P 33.8 1.0 15Carolina & Beckman Synchron CX3/7/9/L 7 26 - 31 P 28.1 1.2 39 - 47 P 43.0 1.6 9 - 13 C 10.7 2.7 28 - 33 P 30.3 1.5 31 - 37 P 34.4 1.0 8Horiba ABX & Horiba ABX 400 8 25 - 30 P 27.8 1.2 38 - 46 P 42.2 1.5 10 - 14 C 11.8 0.6 27 - 33 P 30.1 1.4 30 - 36 P 33.3 1.1 9Medica & Medica Easy RA 9 26 - 32 P 29.1 0.3 39 - 47 P 42.7 0.9 11 - 15 C 13.0 1.1 29 - 34 P 31.6 0.7 32 - 38 P 35.0 0.5 7Ortho Vitros & Ortho Vitros 3600, 5600 10 21 - 25 P 23.3 0.5 33 - 39 P 35.9 0.6 6 - 10 C 7.9 0.3 24 - 28 P 26.1 0.6 25 - 30 P 27.8 0.8 9Ortho Vitros & Ortho Vitros 5,1 FS 11 21 - 25 P 23.3 0.5 32 - 38 P 35.2 1.1 6 - 10 C 7.8 0.4 24 - 28 P 26.0 0.8 25 - 30 P 27.3 1.1 6Ortho Vitros & Ortho Vitros not DT or ECi 12 21 - 26 P 23.5 0.7 33 - 39 P 35.8 0.8 6 - 10 C 8.0 0.0 24 - 29 P 26.2 0.7 25 - 30 P 27.7 0.8 33Roche Cobas & Roche Cobas Integra 13 25 - 30 P 27.6 1.0 38 - 46 P 42.2 1.4 10 - 14 C 11.6 0.6 27 - 33 P 30.1 1.0 30 - 36 P 33.2 1.1 14Roche Cobas & Roche e/c, 1XX, X000, Elec series 14 25 - 30 P 27.6 0.8 38 - 46 P 41.9 1.1 10 - 14 C 11.8 0.4 27 - 33 P 30.1 0.9 30 - 36 P 33.4 1.1 29SDI Biomed & SDI CA-240, 480 15 26 - 31 P 28.8 1.7 40 - 47 P 43.5 0.7 10 - 14 C 12.3 1.0 28 - 34 P 30.9 1.4 32 - 38 P 34.9 1.0 10Sekisui & Beck Olym AU 400/600/5400 16 25 - 30 P 27.8 0.9 39 - 47 P 42.8 1.8 10 - 14 C 12.3 0.7 28 - 34 P 31.0 1.3 31 - 37 P 34.2 1.3 6Sekisui & Roche Cobas Mira series 17 27 - 33 P 30.0 1.0 40 - 49 P 44.5 1.8 12 - 16 C 13.5 0.9 29 - 35 P 32.3 1.3 33 - 39 P 36.0 1.0 4Siemens GLDH-rate & Siemens Advia 18 27 - 32 P 29.2 1.2 40 - 48 P 44.0 1.4 12 - 16 C 13.7 2.5 29 - 35 P 31.8 1.2 29 - 35 P 32.3 6.1 6Siemens GLDH-rate & Siemens Dim RxL, RxL Max 19 26 - 31 P 28.1 0.9 39 - 47 P 43.1 1.3 10 - 14 C 12.1 0.7 28 - 34 P 31.1 1.1 31 - 37 P 34.2 0.6 10Siemens GLDH-rate & Siemens Dimension 20 25 - 31 P 28.0 1.6 39 - 47 P 42.9 2.5 10 - 14 C 12.1 0.8 28 - 33 P 30.4 1.7 30 - 36 P 33.4 1.9 8Siemens GLDH-rate & Siemens Dimension EXL 21 26 - 31 P 28.1 1.0 39 - 47 P 42.9 1.2 10 - 14 C 12.2 1.0 28 - 33 P 30.7 1.0 31 - 37 P 34.0 1.2 59

Siemens GLDH-rate & Siemens Dimension Xpand 22 26 - 31 P 28.1 1.1 39 - 46 P 42.6 1.5 10 - 14 C 12.1 0.8 28 - 33 P 30.6 1.2 31 - 37 P 33.6 1.1 39Sterling Diagnostic & Other spectrophotometers 23 24 - 28 P 26.0 0.8 35 - 42 P 38.7 2.0 12 - 16 C 14.3 0.9 24 - 29 P 26.3 0.7 31 - 37 P 33.7 0.9 6Synermed & Synermed IR500 24 24 - 29 P 26.8 2.2 36 - 43 P 39.8 3.4 9 - 13 C 11.4 0.8 26 - 32 P 29.0 2.3 29 - 35 P 32.4 3.0 5Vital Diagnostic & Vital Diagnostic Eon 25 25 - 29 P 27.0 1.0 38 - 46 P 42.3 1.3 11 - 15 C 12.8 0.4 28 - 33 P 30.3 0.8 31 - 37 P 34.0 0.7 4Vital Diagnostics Envoy & Vital Diagnostic Envoy 26 25 - 31 P 28.0 1.1 39 - 47 P 42.7 1.2 10 - 14 C 12.0 0.7 28 - 33 P 30.6 0.8 31 - 37 P 34.4 1.1 9Initial Grouping by ReagentAbbott 27 26 - 31 P 28.2 0.7 39 - 47 P 43.1 0.9 10 - 14 C 12.0 0.4 28 - 34 P 31.0 0.5 31 - 37 P 34.0 0.8 26Alfa Wassermann 28 26 - 31 P 28.4 1.4 39 - 47 P 43.3 1.9 10 - 14 C 12.3 0.8 28 - 34 P 30.9 1.4 31 - 37 P 34.4 1.8 32Beckman GLDH-rate 29 26 - 31 P 28.5 0.7 39 - 47 P 43.1 1.0 11 - 15 C 13.0 0.2 29 - 35 P 31.7 0.7 31 - 37 P 34.4 0.9 28Beckman Olympus 30 26 - 31 P 28.1 1.1 39 - 46 P 42.6 1.3 10 - 14 C 11.9 0.6 28 - 33 P 30.7 1.0 31 - 37 P 33.7 1.2 58Carolina 31 26 - 31 P 28.4 1.1 39 - 47 P 43.1 1.3 10 - 14 C 11.6 1.9 28 - 33 P 30.6 1.3 31 - 37 P 34.1 1.0 17Horiba ABX 32 25 - 30 P 27.8 1.2 38 - 46 P 42.2 1.5 10 - 14 C 11.8 0.6 27 - 33 P 30.1 1.4 30 - 36 P 33.3 1.1 9Medica 33 26 - 32 P 29.1 0.3 39 - 47 P 42.7 0.9 11 - 15 C 13.0 1.1 29 - 34 P 31.6 0.7 32 - 38 P 35.0 0.5 7Ortho Vitros 34 21 - 26 P 23.5 0.6 33 - 39 P 35.8 0.9 6 - 10 C 8.0 0.2 24 - 29 P 26.2 0.7 25 - 30 P 27.7 0.8 53Pointe Scientific 35 26 - 31 P 28.5 1.5 39 - 47 P 43.3 2.1 10 - 14 C 12.4 1.0 29 - 34 P 31.5 1.6 32 - 38 P 35.1 1.4 11Roche Cobas 36 25 - 30 P 27.6 0.9 38 - 46 P 42.0 1.3 10 - 14 C 11.7 0.5 27 - 33 P 30.2 0.9 30 - 36 P 33.4 1.1 47SDI Biomed 37 26 - 31 P 28.8 1.6 39 - 47 P 43.2 1.1 10 - 14 C 12.2 1.0 28 - 34 P 31.0 1.3 32 - 38 P 34.8 1.0 12Sekisui 38 26 - 31 P 28.7 1.3 40 - 48 P 43.8 2.1 11 - 15 C 13.0 1.0 29 - 34 P 31.6 1.5 31 - 38 P 34.5 1.9 13Siemens GLDH-endpt 39 25 - 31 P 28.0 0.7 40 - 48 P 43.8 1.5 11 - 15 C 12.8 1.9 28 - 33 P 30.3 0.8 31 - 38 P 34.5 1.1 4Siemens GLDH-rate 40 26 - 31 P 28.1 1.1 39 - 47 P 42.9 1.5 10 - 14 C 12.2 1.1 28 - 33 P 30.7 1.2 31 - 37 P 33.7 1.8 122Sterling Diagnostic 41 24 - 29 P 26.9 2.3 36 - 43 P 39.4 2.3 12 - 16 C 14.2 0.9 25 - 29 P 27.0 1.6 31 - 37 P 34.2 1.6 9Synermed 42 25 - 29 P 27.0 2.1 37 - 44 P 40.2 3.2 9 - 13 C 11.3 0.7 27 - 32 P 29.2 2.1 30 - 35 P 32.5 2.8 6Vital Diagnostic 43 25 - 30 P 27.5 1.1 38 - 46 P 42.2 1.2 11 - 15 C 13.2 0.9 28 - 33 P 30.7 0.9 31 - 37 P 33.7 0.9 6Vital Diagnostics Envoy 44 25 - 31 P 28.0 1.1 39 - 47 P 42.7 1.2 10 - 14 C 12.0 0.7 28 - 33 P 30.6 0.8 31 - 37 P 34.4 1.1 9Initial Grouping by Sensitivity or PrincipleAmmonia (NH3) diffusion 48 21 - 26 P 23.5 0.6 33 - 39 P 35.8 0.9 6 - 10 C 8.0 0.2 24 - 29 P 26.2 0.7 25 - 30 P 27.7 0.8 53Glutamate DH-endpoint meths 47 26 - 31 P 28.6 0.8 39 - 47 P 42.8 1.5 11 - 15 C 12.8 1.4 28 - 34 P 30.9 1.1 32 - 38 P 35.0 1.6 14Glutamate DH-rate methods 45 26 - 31 P 28.2 1.8 39 - 47 P 42.9 2.7 10 - 14 C 12.2 1.0 28 - 33 P 30.7 1.2 31 - 37 P 33.9 1.5 408Hypochlorite (ClO)-based 49 24 - 29 P 26.9 2.3 36 - 43 P 39.4 2.3 12 - 16 C 14.2 0.9 25 - 29 P 27.0 1.6 31 - 37 P 34.2 1.6 9Other 46 24 - 29 P 26.4 2.3 37 - 44 P 40.7 3.1 10 - 14 C 11.6 1.0 27 - 32 P 29.3 2.9 29 - 35 P 32.0 3.0 11Total PopulationWhole Population 50 25 - 30 P 27.6 2.4 38 - 46 P 42.0 3.4 10 - 14 C 12.2 8.3 27 - 33 P 30.2 2.5 30 - 36 P 33.3 3.0 498

Uric AcidInitial Grouping by Reagent and InstrumentAbbott Architect & Abbott Architect c, ci, i 1 4.5 - 6.3 P 5.41 0.07 6.9 - 9.7 P 8.29 0.11 0.8 - 1.1 P 0.96 0.16 3.7 - 5.3 P 4.51 0.1 6.5 - 9.2 P 7.88 0.12 21Alfa Wassermann & Alfa Wasser Axcel/Alera 2 4.5 - 6.4 P 5.45 0.24 6.5 - 9.2 P 7.88 0.22 1.2 - 1.7 P 1.42 0.17 3.9 - 5.5 P 4.66 0.27 6.3 - 8.9 P 7.63 0.23 16Beckman Coulter & Beck Coult Unicel DXC 3 3.9 - 5.5 P 4.67 0.06 5.9 - 8.3 P 7.12 0.09 0.5 - 0.7 C 0.57 0.37 3.1 - 4.3 P 3.68 0.06 5.9 - 8.3 P 7.07 0.11 25Beckman Coulter & Beck Olym AU 400/600/5400 4 4.1 - 5.8 P 4.97 0.11 6.6 - 9.3 P 7.95 0.34 0.0 - 0.2 C 0.1 0.0 3.3 - 4.6 P 3.93 0.08 6.3 - 9.0 P 7.65 0.11 4Beckman Olympus & Beck Olym AU 400/600/5400 5 4.1 - 5.8 P 4.95 0.15 6.5 - 9.1 P 7.79 0.17 0.0 - 0.2 C 0.11 0.05 3.2 - 4.5 P 3.88 0.12 6.3 - 8.9 P 7.61 0.19 30Beckman Olympus & Beck Olympus AU 2700 6 4.1 - 5.7 P 4.9 0.07 6.3 - 9.0 P 7.65 0.15 0.0 - 0.2 C 0.1 0.0 3.2 - 4.5 P 3.88 0.08 6.2 - 8.7 P 7.45 0.11 4Beckman Olympus & Beckman AU 480 7 4.2 - 5.9 P 5.01 0.13 6.5 - 9.2 P 7.84 0.11 -0.0 - 0.2 C 0.08 0.04 3.3 - 4.6 P 3.94 0.09 6.3 - 9.0 P 7.65 0.11 8Carolina & Beck Olym AU 400/600/5400 8 3.3 - 4.6 P 3.94 1.98 6.3 - 8.8 P 7.56 0.27 0.0 - 0.2 C 0.12 0.04 3.2 - 4.5 P 3.84 0.14 6.1 - 8.6 P 7.36 0.27 5Carolina & Beckman Synchron CX3/7/9/L 9 3.8 - 5.4 P 4.61 0.1 5.9 - 8.3 P 7.1 0.11 0.2 - 0.4 C 0.33 0.11 3.0 - 4.2 P 3.57 0.09 5.9 - 8.3 P 7.13 0.17 7Horiba ABX & Horiba ABX 400 10 4.0 - 5.6 P 4.76 0.13 6.3 - 8.8 P 7.56 0.18 0.0 - 0.2 C 0.14 0.13 3.1 - 4.4 P 3.74 0.07 6.1 - 8.6 P 7.33 0.16 7Ortho Vitros & Ortho Vitros 3600, 5600 11 4.1 - 5.8 P 4.94 0.08 6.4 - 9.0 P 7.73 0.14 0.4 - 0.6 C 0.5 0.0 3.3 - 4.7 P 3.99 0.1 6.2 - 8.7 P 7.41 0.13 10Ortho Vitros & Ortho Vitros 5,1 FS 12 4.1 - 5.8 P 4.95 0.13 6.3 - 9.0 P 7.65 0.17 0.4 - 0.6 C 0.5 0.0 3.3 - 4.6 P 3.97 0.11 6.1 - 8.6 P 7.32 0.19 6Ortho Vitros & Ortho Vitros not DT or ECi 13 4.1 - 5.8 P 4.94 0.14 6.4 - 9.0 P 7.73 0.34 0.2 - 0.4 C 0.3 0.15 3.3 - 4.7 P 3.98 0.11 6.1 - 8.6 P 7.31 0.2 23Roche Cobas & Roche Cobas Integra 14 4.0 - 5.7 P 4.84 0.1 6.3 - 8.9 P 7.61 0.17 0 - 0.1 C 0.04 0.07 3.1 - 4.4 P 3.77 0.1 6.2 - 8.7 P 7.45 0.12 14Roche Cobas & Roche e/c, 1XX, X000, Elec series 15 4.1 - 5.8 P 4.94 0.11 6.4 - 9.1 P 7.74 0.19 0.0 - 0.2 C 0.14 0.09 3.2 - 4.5 P 3.86 0.11 6.3 - 8.8 P 7.55 0.13 28SDI Biomed & SDI CA-240, 480 16 4.6 - 6.5 P 5.55 0.21 7.2 - 10.1 P 8.64 0.17 0.0 - 0.2 C 0.14 0.08 3.6 - 5.1 P 4.35 0.12 7.1 - 10.1 P 8.61 0.29 11Sekisui & Beck Olym AU 400/600/5400 17 4.4 - 6.2 P 5.28 0.3 6.6 - 9.3 P 7.98 0.33 0.2 - 0.5 C 0.35 0.09 3.6 - 5.0 P 4.3 0.27 6.5 - 9.2 P 7.82 0.43 4Siemens Advia & Siemens Advia 18 4.1 - 5.8 P 4.93 0.16 6.5 - 9.1 P 7.78 0.25 -0.0 - 0.2 C 0.09 0.17 3.2 - 4.6 P 3.91 0.14 5.9 - 8.3 P 7.11 1.13 8Siemens Dimension & Siemens Dim RxL, RxL Max 19 3.9 - 5.5 P 4.73 0.1 6.2 - 8.8 P 7.5 0.15 -0.0 - 0.2 C 0.08 0.04 3.2 - 4.4 P 3.8 0.14 6.1 - 8.5 P 7.3 0.19 10Siemens Dimension & Siemens Dimension 20 4.0 - 5.6 P 4.78 0.25 6.2 - 8.8 P 7.48 0.31 0.0 - 0.2 C 0.12 0.04 3.1 - 4.3 P 3.7 0.17 5.9 - 8.4 P 7.16 0.3 5Siemens Dimension & Siemens Dimension EXL 21 4.0 - 5.6 P 4.77 0.14 6.2 - 8.8 P 7.52 0.16 0.0 - 0.2 C 0.12 0.08 3.2 - 4.4 P 3.8 0.14 6.1 - 8.5 P 7.3 0.15 53Siemens Dimension & Siemens Dimension Xpand 22 4.0 - 5.6 P 4.76 0.14 6.3 - 8.8 P 7.54 0.17 0.0 - 0.2 C 0.11 0.12 3.1 - 4.4 P 3.76 0.16 6.1 - 8.5 P 7.29 0.12 38Vital Diagnostics Envoy & Vital Diagnostic Envoy 23 4.1 - 5.8 P 4.97 0.04 6.6 - 9.3 P 7.95 0.09 0 - 0.1 C 0.0 0.0 3.2 - 4.5 P 3.85 0.11 6.4 - 9.0 P 7.73 0.12 4Initial Grouping by ReagentAbbott Architect 24 4.5 - 6.3 P 5.41 0.07 6.9 - 9.7 P 8.29 0.11 0.8 - 1.1 P 0.96 0.16 3.7 - 5.3 P 4.51 0.1 6.5 - 9.2 P 7.88 0.12 21Alfa Wassermann 25 4.5 - 6.4 P 5.45 0.23 6.5 - 9.2 P 7.86 0.21 1.2 - 1.6 P 1.4 0.17 3.8 - 5.4 P 4.63 0.27 6.3 - 8.9 P 7.64 0.22 18Beckman Coulter 26 3.9 - 5.5 P 4.72 0.13 6.0 - 8.5 P 7.25 0.34 0.5 - 0.7 C 0.55 0.39 3.1 - 4.4 P 3.72 0.12 5.9 - 8.4 P 7.16 0.25 34

Beckman Olympus 27 4.1 - 5.8 P 4.96 0.14 6.5 - 9.1 P 7.78 0.17 0.0 - 0.2 C 0.1 0.04 3.2 - 4.6 P 3.89 0.11 6.3 - 8.9 P 7.6 0.18 43Carolina 28 3.7 - 5.2 P 4.45 1.04 6.0 - 8.4 P 7.22 0.28 0.1 - 0.3 C 0.22 0.11 3.1 - 4.3 P 3.69 0.19 5.9 - 8.4 P 7.14 0.23 20Horiba ABX 29 4.0 - 5.6 P 4.76 0.13 6.3 - 8.8 P 7.56 0.18 0.0 - 0.2 C 0.14 0.13 3.1 - 4.4 P 3.74 0.07 6.1 - 8.6 P 7.33 0.16 7Ortho Vitros 30 4.1 - 5.8 P 4.95 0.13 6.4 - 9.0 P 7.73 0.29 0.3 - 0.5 C 0.38 0.15 3.3 - 4.7 P 3.98 0.11 6.1 - 8.6 P 7.35 0.2 40Pointe Scientific 31 4.2 - 5.9 P 5.01 0.27 6.3 - 8.9 P 7.6 0.44 0.0 - 0.2 C 0.1 0.1 3.6 - 5.1 P 4.36 1.22 6.4 - 8.9 P 7.65 0.44 11Roche Cobas 32 4.1 - 5.8 P 4.92 0.12 6.4 - 9.0 P 7.72 0.2 -0.0 - 0.2 C 0.09 0.1 3.2 - 4.5 P 3.85 0.12 6.3 - 8.8 P 7.54 0.15 46SDI Biomed 33 4.6 - 6.5 P 5.55 0.19 7.2 - 10.1 P 8.64 0.15 0.0 - 0.2 C 0.12 0.08 3.6 - 5.1 P 4.35 0.12 7.1 - 10.0 P 8.58 0.29 13Sekisui 34 4.4 - 6.3 P 5.36 0.35 6.9 - 9.7 P 8.31 0.42 0.2 - 0.4 C 0.28 0.11 3.7 - 5.2 P 4.44 0.39 6.8 - 9.6 P 8.22 0.52 10Siemens Advia 35 4.1 - 5.8 P 4.93 0.16 6.5 - 9.1 P 7.78 0.25 -0.0 - 0.2 C 0.09 0.17 3.2 - 4.6 P 3.91 0.14 5.9 - 8.3 P 7.11 1.13 8Siemens Dimension 36 4.0 - 5.6 P 4.76 0.14 6.2 - 8.8 P 7.52 0.17 0.0 - 0.2 C 0.11 0.09 3.1 - 4.4 P 3.78 0.15 6.1 - 8.5 P 7.29 0.16 106Sterling Diagnostic 37 4.1 - 5.8 P 4.98 0.34 6.5 - 9.1 P 7.82 0.48 0.0 - 0.2 C 0.1 0.0 3.6 - 5.0 P 4.28 0.16 6.2 - 8.8 P 7.48 0.6 5Vital Diagnostics Envoy 38 4.1 - 5.8 P 4.97 0.04 6.6 - 9.3 P 7.95 0.09 0 - 0.1 C 0.0 0.0 3.2 - 4.5 P 3.85 0.11 6.4 - 9.0 P 7.73 0.12 4Initial Grouping by Sensitivity or PrincipleEndpoint-uncorrected 39 4.3 - 6.1 P 5.24 0.42 6.8 - 9.5 P 8.15 0.75 0.1 - 0.3 C 0.16 0.14 3.6 - 5.0 P 4.31 0.63 6.6 - 9.2 P 7.9 0.79 54Endpt-corrected(bic or SB) 40 4.2 - 5.9 P 5.08 0.27 6.5 - 9.2 P 7.85 0.31 0.4 - 0.6 C 0.51 0.48 3.4 - 4.8 P 4.1 0.35 6.3 - 8.8 P 7.56 0.27 139Rate 41 4.0 - 5.6 P 4.76 0.37 6.2 - 8.8 P 7.49 0.29 0.1 - 0.3 C 0.19 0.24 3.1 - 4.4 P 3.78 0.15 6.1 - 8.6 P 7.31 0.23 207Other 42 4.2 - 5.9 P 5.03 0.26 6.5 - 9.1 P 7.78 0.34 0.4 - 0.6 C 0.47 0.51 3.4 - 4.7 P 4.04 0.33 6.2 - 8.8 P 7.51 0.34 8Total PopulationWhole Population 43 4.1 - 5.8 P 4.93 0.4 6.4 - 9.0 P 7.71 0.5 0.4 - 0.6 C 0.46 0.95 3.3 - 4.6 P 3.96 0.39 6.2 - 8.8 P 7.5 0.55 408