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Agri Biotech Foundation PJTASU Campus, Rajendranagar-500030
Email: [email protected], www.abfindia.org
REPORT
On
Report on “Summer Internship in Biotechnology for Undergraduates”
1st -31st May, 2015
Report on “Summer Internship in Biotechnology for Undergraduates”
1st -31st May, 2015
As part of its efforts to develop human resources in the field of agribiotechnologies, Agri
Biotech Foundation organizes one month summer internship for undergraduate students every
year. This year also the foundation has conducted “Summer Internship in Biotechnology for
Undergraduates” during 1st – 31st May, 2015. A total of 12 students participated from three
different colleges Viz; Aurora Degree College, Hyderabad, Reddy Women’s College,
Hyderabad, St.Ann’s Degree College, Hyderabad (Annexure 1).
The “Summer Internship in Biotechnology for Undergraduates” inaugurated on 1st May,
2015 by Prof. G.Pakki Reddy, Executive Director, ABF, Dr. J.S. Bentur, Subject Matter
Specialist, ABF, Dr. Udaykiran Avasati, Young Scientist, ABF and Mr. J.Vijaya Naresh,
Research Scholar, ABF besides the other senior faculty members Dr. G. Mallikarjuna, Assistant
Professor, Dr. S.K.Z. Ali, Assitant Professor, Dr. V. Sandhya, Assitant Professor and Mr. M.
Gopala Krishna, Research Associate. Apart from the senior faculty members, Research students
Mrs. Bindu Prathyusha, Mrs. Chandana, Mrs. Mounika, Mrs. Padmaja, Mrs. Suma, Mr. Chandra
Teja, Mr. Kishore, Mr. Nihar, Mr. Prasanna, Mr. Shiva, Mr. Santosh and also participated.
Inaguaral address by Prof. G. Pakki Reddy, ED, ABF Dr. J.S. Bentur, SMS, ABF addressing the gathering
The main objective of this programme is to motivate the youth to take up life sciences as
their career and to impart the knowledge and skill in the field of biotechnology particularly in
agricultural biotechnology. To meet these objectives, the programme has divided into four
components viz; (1) Plant tissue culture (2) Plant transformation and Genetic engineering (3)
Microbiology and (4) Molecular marker technology. For each component one week time has
allocated, within that, each day of the respective component started with knowledge based
theoretical sessions by expert faculty members of the foundation followed by individual hands
on training on project allocated to each candidate based on their interested areas. Further, each
student mentored by individual mentor to enhance one to one learning.
Lecture by Dr. J.S. Bentur, SMS, ABF Lecture by Ms. Divya, SRF, IIRR
Lecture by Dr. V. Sandhya, Assitant Professor, ABF Lecture by Mr. Chandra Teja, SRF, ABF
In the first week of training programme knowledge based theoretical sessions followed
by hands on training has given in the area of plant tissue culture aspects by Mr. M. Gopala
krishna and Mrs. Padmaja Rani. During this session students have trained in Media preparations,
stocks preparation, maintaining of stocks, sterilizing media, explant preparation, bana and teak
micropropagation. Apart from these, students have also trained in green house activities like soil
mixing, pot filling, transplantation of invitro rooted saplings into soil and application of
fertilizers.
Students in greenhouse activities
In the second week of the session students have focused on plant transformation and
genetic engineering techniques. Dr. G. Mallikarjuna, Dr. Udaykiran Avasathi, Mr. J. Vijaya
Naresh, and Mr. Santosh have given insights into various aspects of plant transformation and
genetic engineering techniques. Majorly students have trained on genomic DNA and plasmid
DNA isolations. Further, students have also trained in cloning strategies and performed
restriction digestion and ligation of plasmids and transferred them into E.Coli, Agro
transformations through heat shock and electroporation methods respectively. Genetic
transformation of ground and rice has also demonstrated. Apart from these, students have also
performed chlorophyll estimations from leaves of different crop plants.
Hands on training on Molecular Biology experiments by interns
In the microbiology session, Dr. S.K.Z. Ali, Dr. V. Sndhya, Mr. Chandra Teja, Mrs.
Mounica, Mr. Prasad and Mr. Kishore have given knowledge based insights about portray
technology, bio fertilizers, biopesticides production and their benefits. Besides, students have
also performed antibiotic sensitivity test, motility test and isolated of genomic DNA from Gram
–ve and +ve bacteria. They also performed molecular tests like PCR and biochemical tests like
IMViC reactions.
Interns Undergoing training in microbiology related experiments
In the molecular markers session, Dr. J.S. Bentur, Mrs. Prathyusha and Mr. Nihar Sahoo
has explained basics in molecular markers and their use in crop improvement, Mapping
population and linkage analysis and Development of new markers for black gram respectively.
Apart from these Mrs. Suma Latha spoke about use of molecular markers in transgenics
identification and Ms. Divya from Indian Institute of Rice Research gave insights in
identification of candidate genes and gene specific markers. Further, students have trained in
setting up of Gradient PCR, Bulk Segregant Analysis, screening for plant hopper resistance,
maintaining/rearing insects especially WBPH and BPH.
Students in insect rearing facility Hands on skill on molecular markers
Besides knowledge based theoretical sessions, skill based capacity building was given to
the interns by giving individual projects to each student based upon their area of interest
(Annexure 2). On completion of the project students have also gave power point presentations on
their respective projects.
Ms. Sindhura from St. Ann’s college has given a project entitled “Micropropagation of Bana
(Musa paradisiacal)” in the area of plant tissue culture. As part of her project she has learnt
sucker preparation, surface sterilization and initiation of suckers and regeneration and
multiplication of banana saplings.
In the area of plant transformation/transgenics Ms. Lahari, Ms. Sushmitha, Ms. Kavitha
and Mr. Yeshwanth Reddy were given a projects on Rice, Green gram and groundnut
transformations against abiotic stress tolerance respectively. Ms. Lahari has trained in dehusking
of rice grains, surface sterilization, media preparation for callus induction, Agro culture
preparation, infection of calli with agrobacterium, co-cultivation and sub-culturing of infected
calli on resting medium. Similarly, Ms. Sushmitha has trained in explant preparation from green
gram, infection of half cotyledonary node explants with agrobacterium culture, co-cultivation
followed by selection of co-cultivated explants on kanamycin containing medium. Ms. Kavitha
and Mr. Yeshwanth Reddy did their project on “Development of transgenic groundnut for
improving abiotic stress tolerance”. They have used de-embryonated cotyledons from mature
seeds of ground nut as explants for infection. Infected explants undergone for co-cultivation
followed by selection under hygromycin containing media.
Ms. Niharika, Ms. Rashmitha, Mr. Aman Jayaker and Mr. Nihar were given project work
in the field of microbiology. Ms. Niharika and Mr. Nihar has worked on “Isolation and
characterization of Aztobacter Spp.” and “Isolation and Characterization of Psedomonas
fluroscence Spp” respectively. As part of the project they have collected rhizospear soil samples
for isolation of Aztobacter/Pseudomonas. Selected isolates screened for PGPR traits like
ammonia, HCN and IAA production besides Phosphate solubilization activity. Apart from these
they have also trained in molecular techniques like genomic DNA isolations and PCR. Ms.
Rashmitha worked on “Isolation and Characterization of Endophytic bacteria”. She has isolated
endophytes from different parts of plant samples and tested them for various PGPR activities.
Mr. Aman Jayakar has given the project entitled “Isolation and Characterization of metarizium
and Beaverium Spp.
In the area of Molecular Markers under individual mentorship Ms. Asra has given the
project entitled “Identification of resistant varieties against WBPH in rice”. As part of her project
she used both phenotyping and genotyping methods for identifying WBPH resistant rice
varieties. In phenotyping she has done standand seedbox screening test for damage score for
WBPH and honey dew test to check the feeding rate in different genotypes. In genotyping she
has screened different genotypes with rice SSR markers to check polymorphism between them.
Similarly, Mr. Surya has also given the project work entitled “Identification of resistant varieties
against BPH in rice”. For this, he also did both phenotyping and genotyping methods. In
phenotyping he has done standand seedbox screening test for damage score for BPH and honey
dew test to check the feeding rate in different genotypes. In genotyping he has done DNA
isolation from rice leaves and screened different genotypes with rice SSR markers to check
polymorphism between them. Mr. Vijay has given the project “Resistance to Mungbean Yellow
Mosaic (MYMV) in blackgram varieties”. In his project he has took DUS characters of two
blackgram genotypes. He also collected MYMV infected sample from the field and isolated
DNA from the infected plant leaf samples for screening the samples with virus specific markers.
He also used SSR markers for screening genotypes for checking parental polymorphism.
In addition to these knowledge based theoretical insights and hands on skill development
through individual projects in the afore mentioned four major areas, Mr. Santosh has given
insights in Bio informatics tools like NCBI, BLAST, CLUSTAL W and Primer 3 software’s.
Similarly, Mrs. Prathyusha has given a lecture “Use of Biostatistics in Agriculture Sciences”
Students have taught about the use of Bio Statistics concepts and tools like Mean, Median,
Avarage, Annova, Chi square, correlation and regression in biological data analysis. Apart from
these, students have also made aware of Microsoft Office tools like micro soft excel, power point
slide preparations. Mr. Prasanna Kumar, SRF of the Foundation has demonstrated
vermicomposting technology and its benefits. Further, Dr. G. Mallikarjuna has given career
guidance for their future.
Hands on skill on portray technology and vermicomposting technology
Students have participated in science fair and presented their ideas in the form of posters
on topics like Genetically modified crops for pollution control and therapeutics, DNA finger
printing, Somatic hybridization, anti ageing components in Rosa indica, Food adulterations,
benefits of top soil and light, Decomposition of dead body, Rising of vegetable seedlings using
pro-tray technologies and Organic farming. Further, students have also undergone for IQ test.
Poster presentations by interns
At the end of the summer internship programme each student presented their project
emphasizing on the technical skills acquired during the project.
Students presenting their project work
The summer internship programme ended on 30th May, 2015 by valedictory function. Dr.
J.S. Bentur, SMS, ABF and Dr. G. Mallikarjuna, Assistant Professor, ABF headed these
programme. Mr. J. Vijaya Naresh summarized the entire course activities. Following these Dr.
J.S. Bentur distributed certificates to each student along with a book of Swami Vivekanada and
prizes to the students who performed well in during the entire course based on the different
criteria like individual student’s poster presentation, power point presentation of their project,
attendance, IQ test and their respective mentor evaluations.
Group Photo of the interns along with the faculty Prof. G. Pakki Reddy, ED, ABF, interacting with the interns members of the foundation
Mr. J. Vijaya Naresh summarizing the course details in Students sharing their experiences of the course in the valedictory the valedictory session
Dr. J.S. Bentur, Distributing the certificates and prizes to the interns
Cultural activities by the students at ABF
Key points of the Summer Internship in Biotechnology for Undergraduates - 2015
All in all total 25hr has spent for knowledge based theoretical sessions and 102hr for skill
based hands on training activities, which itself represented we have given more emphasis
for skill based hands on training.
Based on the pre and post evaluations by questionnaires on an average students rated
themselves 51% more after the attending the programme for their knowledge.
Students on an average gained 147% more practical and theoretical knowledge.
All students expressed their satisfaction in the valedictory session as well as in the
interactive session with the Executive Director, ABF. However, four students suggested
for increasing the course duration from one month to two months. Some of the students
have suggested for winter short term winter school like the summer internship
programme on techniques.
Annexure 1
List of participants:
S.No Name and college
1 M. Vijay Krishna, Aurora’s Degree College
2 C. Sindhura, St. Ann’s Degree College for Women
3 E. Lahari, St. Ann’s Degree College for Women
4 Syeda Asra Banu, Aurora’s Degree College
5 Susmitha Billapati, R.B.V.R.R Womens College
6 K. Surya Chander Rao, Aurora’s Degree College
7 P. Aman Jayaker. Aurora’s Degree College
8 T. Yashwanth Reddy, Aurora’s Degree College
9 C. Sarath Chand Nihar, Aurora’s Degree College
10 A. Kavitha, Aurora’s Degree College
11 R. Niharika, R.B.V.R.R Womens College
12 K. Rashmitha, R.B.V.R.R Womens College
Annexure: 2
List of projects entitle to the students:
Name of the Mentee Name of the Mentor Project Title
M. Vijay Krishna, Aurora’s Degree College
Mrs. Prathyusha “Resistance to Mungbean Yellow Mosaic
(MYMV) in blackgram varieties”
C. Sindhura, St. Ann’s Degree College for Women
Mrs. Padmaja “ Micropropagation of Banana”
E. Lahari, St. Ann’s Degree College for Women
Mr. Vijaya Naresh “ Transgenics for crop improvement”
Syeda Asra Banu, Aurora’s Degree College
Dr. J.S. Bentur “Identification of resistant varieties against
WBPH in rice”.
Susmitha Billapati, R.B.V.R.R Womens College
Mr. Gopala Krishna “Genetic transformation of Green gram for
herbicide resistance”
K. Surya Chander Rao, Aurora’s Degree College
Mr. Nihar Sahoo “Identification of resistant varieties against
BPH in rice”
P. Aman Jayaker. Aurora’s Degree College
Mr. Chandra Teja “Isolation and Characterization of metarizium
and Beaverium Spp”
T. Yashwanth Reddy, Aurora’s Degree College
Dr. G. Mallikarjuna “Genetic transformation of Ground nut for
abiotic stress tolerance”
C. Sarath Chand Nihar, Aurora’s Degree College
Mr. Sai Prasad “Isolation and Characterization of Psedomonas
fluroscence Spp”
A. Kavitha, Aurora’s Degree College
Mr. Santosh “Genetic transformation of Ground nut for
abiotic stress tolerance”
R. Niharika, R.B.V.R.R Womens College
Mrs. Mounika “Isolation and characterization of Aztobacter
Spp.”
K. Rashmitha, R.B.V.R.R Womens College
Mr. Kishore “Isolation and Characterization of Endophytic
bacteria”.