advantages of dictyostelium
TRANSCRIPT
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Advantages of Dictyostelium
• Simple organism • Rapid doubling time • Contains many signaling networks found in
larger eukaryotes • Genetically pliable • Genome is sequenced • Differentiates and develops when starved
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Dictyostelium life cycle is complete in 24 hours
• Unicellular eukaryote, social amoeba • Vegetative condition: individual cell • Starved condition: cells aggregate, fruiting body
Dictybase.org
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Phosphorylation of Actin-related Protein (Arp2) Is Required for
Normal Development and cAMP Chemotaxis in Dictyostelium
Choi, Thomason, Zaki, Insall and Barber
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Making Phosphorylation Mutants of Arp2
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Chemotaxis Assay
WT Phosphorylation mutant
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• Speed: rate of distance travelled over time; measured in µm/min
• Directionality: ability to stay on path; net distance traveled /
total distance
Value = 1 0 >Value > 1
Chemotaxis Assay
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Arp2 Phosphorylation Regulates Chemotaxis
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Arp2 Phosphorylation Regulates Actin Polymerization
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Chemotaxis Assay
WT Phosphorylation mutant
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Arp2 Phosphorylation is Required for Proper Development
Subm
erge
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lture
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Arp2 Tyrosine Phosphorylation Increases During Development
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Prolonged Development Rescues Chemotaxis Defect
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Prolonged Development does not Rescue Actin Polymerization Defect
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Arp2 Phosphorylation Controls Pseudopod Behavior
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Arp2 Phosphorylation Plays Minor Role in Folate Chemotaxis
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Arp2 Phosphorylation is not Important for Endocytosis
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Arr2 Phosphorylation Regulates Chemotaxis Speed