adult neurogenesis in the female mouse hypothalamus ... · pdf filetvx ,pdjhvuhvxowlqjiurph...

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Accepted manuscripts are peer-reviewed but have not been through the copyediting, formatting, or proofreading process. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Copyright © 2016 the authors This Accepted Manuscript has not been copyedited and formatted. The final version may differ from this version. Research Article: New Research | Integrative Systems Adult neurogenesis in the female mouse hypothalamus: Estradiol and high fat diet alter the generation of newborn neurons expressing estrogen receptor # Estradiol and diet alter generation of new ER# neurons in adult female hypothalamus Elizabeth P. Bless 1 , Jane Yang 1 , Kalpana D. Acharya 1 , Sabin A. Nettles 1 , Fair M. Vassoler 2 , Elizabeth M. Byrnes 2 and Marc J. Tetel 1 1 Neuroscience Program, Wellesley College, Wellesley, MA 02481, USA 2 Department of Biomedical Sciences, Cummings School of Veterinary Medicine, Tufts University, Grafton, MA 01536, USA DOI: 10.1523/ENEURO.0027-16.2016 Received: 11 February 2016 Revised: 22 August 2016 Accepted: 23 August 2016 Published: 29 August 2016 Author Contributions: EPB, EMB and MJT designed research; EPB, JY, KDA, SAN and FMV performed research; EPB, KDA, SAN, FMV, EMB and MJT analyzed data; EPB, EMB and MJT wrote the paper Funding: NIDDK R01 DK61935 Funding: NIDDK Re-entry Award Conflict of Interest: Authors report no conflict of interest. Correspondence should be addressed to Marc J. Tetel, Neuroscience Program, Wellesley College, 106 Central Street, Wellesley, MA 02481. E-mail: [email protected] Cite as: eNeuro 2016; 10.1523/ENEURO.0027-16.2016 Alerts: Sign up at eneuro.org/alerts to receive customized email alerts when the fully formatted version of this article is published.

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Page 1: Adult neurogenesis in the female mouse hypothalamus ... · PDF filetvx ,PDJHVUHVXOWLQJIURPH ... the female mouse hypothalamus: Estradiol and high fat diet alter the generation of newborn

Accepted manuscripts are peer-reviewed but have not been through the copyediting, formatting, or proofreadingprocess.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International(http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in anymedium provided that the original work is properly attributed.

Copyright © 2016 the authors

This Accepted Manuscript has not been copyedited and formatted. The final version may differ from this version.

Research Article: New Research | Integrative Systems

Adult neurogenesis in the female mouse hypothalamus: Estradiol and high fat dietalter the generation of newborn neurons expressing estrogen receptor #

Estradiol and diet alter generation of new ER# neurons in adult female hypothalamus

Elizabeth P. Bless1, Jane Yang1, Kalpana D. Acharya1, Sabin A. Nettles1, Fair M. Vassoler2, Elizabeth M. Byrnes2

and Marc J. Tetel1

1Neuroscience Program, Wellesley College, Wellesley, MA 02481, USA2Department of Biomedical Sciences, Cummings School of Veterinary Medicine, Tufts University, Grafton, MA01536, USA

DOI: 10.1523/ENEURO.0027-16.2016

Received: 11 February 2016

Revised: 22 August 2016

Accepted: 23 August 2016

Published: 29 August 2016

Author Contributions: EPB, EMB and MJT designed research; EPB, JY, KDA, SAN and FMV performedresearch; EPB, KDA, SAN, FMV, EMB and MJT analyzed data; EPB, EMB and MJT wrote the paper

Funding: NIDDKR01 DK61935

Funding: NIDDKRe-entry Award

Conflict of Interest: Authors report no conflict of interest.

Correspondence should be addressed to Marc J. Tetel, Neuroscience Program, Wellesley College, 106Central Street, Wellesley, MA 02481. E-mail: [email protected]

Cite as: eNeuro 2016; 10.1523/ENEURO.0027-16.2016

Alerts: Sign up at eneuro.org/alerts to receive customized email alerts when the fully formatted version of thisarticle is published.

Page 2: Adult neurogenesis in the female mouse hypothalamus ... · PDF filetvx ,PDJHVUHVXOWLQJIURPH ... the female mouse hypothalamus: Estradiol and high fat diet alter the generation of newborn

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Data Structure Type of Test p values a Normally distributed ANOVA 0.022 b Normally distributed ANOVA 0.002 c Normally distributed ANOVA 0.001 d Normally distributed ANOVA 0.002 e Normally distributed ANOVA 0.003 f Normally distributed ANOVA 0.003 g Normally distributed Tukey’s HSD post hoc 0.026 h Normally distributed Tukey’s HSD post hoc 0.021 i Normally distributed Tukey’s HSD post hoc 0.001 j Normally distributed ANOVA 0.02 k Normally distributed ANOVA 0.019 l Normally distributed ANOVA 0.044 m Normally distributed Tukey’s HSD post hoc 0.11 n Normally distributed Tukey’s HSD post hoc 0.15 o Normally distributed Tukey’s HSD post hoc 0.15 p Normally distributed Tukey’s HSD post hoc 0.11 q Normally distributed ANOVA 0.023 r Normally distributed ANOVA 0.014 s Normally distributed ANOVA 0.04 t Normally distributed Tukey’s HSD post hoc 0.152 u Normally distributed Tukey’s HSD post hoc 0.14 v Normally distributed Tukey’s HSD post hoc 0.145 w Normally distributed Tukey’s HSD post hoc 0.096 x Normally distributed ANOVA 0.043 y Normally distributed ANOVA 0.054 z Normally distributed ANOVA 0.048 aa Normally distributed ANOVA <0.001 bb Normally distributed Tukey’s HSD post hoc 0.014 cc Normally distributed Tukey’s HSD post hoc 0.002 dd Normally distributed Tukey’s HSD post hoc <0.001 ee Normally distributed Tukey’s HSD post hoc 0.02 ff Normally distributed Tukey’s HSD post hoc <0.001 gg Normally distributed Student’s t-test 0.027

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