adam david gracz
DESCRIPTION
NF2/Merlin Normal Functions and implications in cancer biology. Adam David Gracz. Normal Function. Tumor Suppressor. NF2 = gene Merlin = protein…a.k.a. schwannomin. Tumor suppressive ability comes from partial inhibition of Ras/Rac signaling (phosphorylates in the presence of Ras/Rac). - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/1.jpg)
![Page 2: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/2.jpg)
• NF2 = gene• Merlin = protein…a.k.a.
schwannomin
Tumor Suppressor
• Tumor suppressive ability comes from partial inhibition of Ras/Rac signaling (phosphorylates in the presence of Ras/Rac).
![Page 3: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/3.jpg)
• Similar to ERM family of proteins : membrane-cytoskeleton linking proteins
• Strong evidence of a role in cell motility and proliferation
Sun, et. al. Protein 4.1 tumor suppressors: getting a FERM grip on growth regulation. Journal of Cell Science 2002: 115, 3991-4000
![Page 4: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/4.jpg)
• Is considered functionally active in “closed” state – N- (FERM domain) and COOH-terminals self-associate.
• Serine phosphorylation at COOH-terminus prevents self-association, Merlin is in “open state” and therefore functionally inactive.
Sun, et. al. Protein 4.1 tumor suppressors: getting a FERM grip on growth regulation. Journal of Cell Science 2002: 115, 3991-4000
![Page 5: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/5.jpg)
• Helps regulate signaling in RhoGTPases (Rho, Rac, and Cdc42). These factors control cell motility and matrix adhesions.
Sun, et. al. Protein 4.1 tumor suppressors: getting a FERM grip on growth regulation. Journal of Cell Science 2002: 115, 3991-4000
![Page 6: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/6.jpg)
• Neurofibromatosis type II is autosomal dominant• Follows Knudson’s “two-hit” hypothesis:
– One allelic mutation from parent– One somatic allelic mutation
![Page 7: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/7.jpg)
• Loss of function also observed in sporadic tumors (i.e. non-neurofibromatosis type II patients).
• Proposed model of tumor suppressive function involves phosphorylation of Merlin by kinase cascade initiated through Rho/Rac
![Page 8: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/8.jpg)
Sun, et. al. Protein 4.1 tumor suppressors: getting a FERM grip on growth regulation. Journal of Cell Science 2002: 115, 3991-4000
![Page 9: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/9.jpg)
Increased cell growth/survival/motility!
Sun, et. al. Protein 4.1 tumor suppressors: getting a FERM grip on growth regulation. Journal of Cell Science 2002: 115, 3991-4000
![Page 10: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/10.jpg)
• Mouse models that are doubly deficient of NF2 (genotype NF2-/-) exhibit lethality in the embryonic stage (days 6.5-7: high specificity).
Wild-type Homozygousmutant
McClatchey, et al. The Nf2 tumor suppressor gene product is essential for extraembryonic development immediately prior to gastrulation. Genes & Dev. 1997 11: 1253-1265
![Page 11: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/11.jpg)
• Mouse models with one mutated allele of NF2 (genotype NF2+/-) exhibit extensive, multi-systemic tumors with a high rate of metastasis.
![Page 12: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/12.jpg)
• Tissue deficient of Merlin loses ability to form cadherin mediated cell-cell junctions.
• Cells expressing aberrant Ras exhibit contact inhibition. This property can be “rescued” by the addition of wild-type Merlin.
McClatchey, Merlin and ERM proteins: unappreciated roles in cancer development? Nature Reviews Cancer Nov. 2003 877-883
![Page 13: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/13.jpg)
Bilateral Vestibular Schwannoma(Acoustic neuroma)
Xiao, et. al. Chinese Medical Journal, 2007, Vol. 120 No. 16 : 1456-1459
Classic manifestation = MISME: Multiple Inherited Schwannomas, Meningiomas, Ependymomas
Meningioma
Tuburculum Sella Meningioma: http://www.cornellneurosurgery.org/endoscopicskullbase/Meningiomas.htm
![Page 14: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/14.jpg)
Spinal ependymoma – intradural-extramedullary
Mesothelioma
Ependymoma of the Lumbar Spine:http://www.columbiaspine.org/patients/disease/spinal_tumors.html
History: Cough: http://www.radiology.vlahos.org/45391/47473.html
![Page 15: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/15.jpg)
• Observation if tumors diagnosed early• Cochlear implants to compensate for hearing loss
(vestibular schwannomas) • Surgical resection or decompression• Stereotactic radiosurgery
![Page 16: Adam David Gracz](https://reader031.vdocuments.us/reader031/viewer/2022013012/56814dcd550346895dbb268d/html5/thumbnails/16.jpg)
• Clinical trial for vatalanib in progress at Northwestern University:
-patient must be an adult-works as a protein tyrosine kinase inhibitor
targeting tumor angiogenesis – nonspecific to NF2/Merlin
• Precise pathway and interaction with other signaling cascades remains unclear.